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Citations for Millipore Sigma :
351 - 400 of 1008 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... pH ~7.4) and permeabilized for 10 min in PEM + 0.1% NP-40 Alternative (Millipore, 492016) + 6.6 nM Alexa Fluor™ 488 Phalloidin (and 0.2x Tubulin Tracker Deep Red (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... the pellet was carefully lysed in 50 µl resuspension buffer supplemented with NP-40 (Sigma), Tween-20 and Digitonin (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Physiology 2022Quote: Protein samples were solubilized in 1% NP-40 lysis buffer with 4% protease inhibitors (Sigma) and centrifuged for 14,000 g for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... containing 2 µL of lysis buffer (0.2% Triton-X 100, 0.2% NP-40 (Sigma Aldrich), 1 U/µL RNaseOUT (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: Washed pellets were resuspended in 200 μL of Buffer B (1% NP-40 (Sigma-Aldrich), 20 mM HEPES ...
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bioRxiv - Neuroscience 2019Quote: ... 1% NP-40 supplemented with protease inhibitor cocktail set V EDTA free (Millipore, Billerica, MA) and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, Protease inhibitor cocktail (Sigma), 0.1% SDS and 50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... or 400µg NP-PCC (4-hydroxy-3-nitrophenylacetyl (Biosearch) conjugated to pigeon cytochrome C (Sigma)) mixed with adjuvant based on Monophosphoryl Lipid A ...
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bioRxiv - Cell Biology 2020Quote: ... 2mM EDTA and 0,5% NP-40 supplemented with a cocktail of protease inhibitors (Sigma-Aldrich). Cells were lysed during 15min at 4°C on a rotating-wheel and centrifugated at 13000rpm during 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... NPs were purified by ultracentrifugation over a filter (MWCO 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg ml− 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% NP-40 and 5 mM -mercaptoethanol) supplemented with 0.4 μM AEBSF (Sigma, Cat# A8456), 0.4 μM Benzamidine (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... the NPs were purified by ultracentrifugation over a filter (molecular weight cutoff 10 kDa; Millipore Amicon Ultra-15 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% (v/v) NP-40) containing 1x SIGMAFAST™ protease inhibitor cocktail (Sigma-Aldrich, S8830). For each well/condition ...
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bioRxiv - Cell Biology 2023Quote: ... washed 3 times for 5 min in PBS and permeabilized with 0.5% NP-40 (Sigma) in PBS for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% NP-40) supplemented with 100 mM PMSF and 100 mM DTT (Dithiothreitol, Sigma Aldrich). Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... NP-40 cell lysates were incubated with 10 U/μL of benzonase (Millipore, 71205-3) for 4 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40) was supplemented with EDTA-free Halt protease and phosphatase inhibitor cocktail (Sigma) and applied directly to PBS-rinsed Toxoplasma-infected monolayers ...
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bioRxiv - Plant Biology 2024Quote: ... 0.25% NP-40) containing 1mM PMSF and 1X cOmplete® EDTA-free protease inhibitor (Sigma), followed by centrifugation for 10 min at 15,800 x g at 4°C ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2019Quote: ... virus dilutions were overlaid with 1 ml of Opti-MEM [1% Carboxymethylcellulose (Sigma), 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... individuals performed a nasopharyngeal swab into virus transport medium (VTM; MWE Sigma-Virucult), gave details on any recent infection episodes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 0,5% NP-40 supplemented with 1:300 mammalian protease inhibitor cocktail (Sigma). FCS measurements were performed using a Zeiss LSM780 confocal microscope using a C-Apochromat 40x/1.2 W Corr M27 objective and using a water-phase immersion oil Immersol W 2010 (Zeiss) ...