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Citations for Addgene :
201 - 250 of 770 citations for Rat Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: A viral cocktail containing AAV2/5.GfaABC1D GCaMP6f (3 × 1012 gc/ml, Addgene) for astrocyte Ca2+ detection or AAV9.hSyn.GCaMP6f (3 × 1012 gc/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 1-5 μg of CS2-plasmid containing the ORF for Cas9 (Addgene #51307) or H2B-RFP was linearized by Not1 endonuclease digestion ...
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bioRxiv - Genetics 2019Quote: ... The plasmid containing Enhanced SpCas9 (version 1.1, Addgene #71814, Slaymaker et al., 2016) was a generous gift of Carine Giovannangeli from the Museum National d’Histoire Naturelle ...
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bioRxiv - Synthetic Biology 2019Quote: ... The fragment containing the LbCpf1 coding sequence was amplified from pY016 hLbCpf1 (Addgene plasmid # 69988 ...
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bioRxiv - Biochemistry 2020Quote: We used the human SREBP-1c cDNA containing vector pQCXIN (Addgene, USA, 631514) as a template to generate 2x Flag tagged ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids containing coding regions for the FPs mKate (mKate-H4-23, Addgene 56061), mRUBY (GCaMP6f-mRUBY ...
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bioRxiv - Immunology 2020Quote: ... a vector containing human IgG3 was purchased from Addgene (pVITRO1-102.1F10-IgG3/λ) and then cloned into a vector for recombinant IgG expression that we previously engineered [59].
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bioRxiv - Neuroscience 2021Quote: ... and donor DNA plasmid containing a neomycin-resistance cassette (adapted from Addgene, PL552). Transfected cells were selected with neomycin for one week ...
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bioRxiv - Cancer Biology 2022Quote: ... PC-3 cells were transduced with lentivirus containing lentiCas9-Blast construct (Addgene #52962) and selected with growth medium containing 4 µg/ml blasticidin for approximately one week ...
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bioRxiv - Neuroscience 2022Quote: The AAV-2 ITR containing plasmids pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (Addgene plasmid #104495 ...
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bioRxiv - Neuroscience 2023Quote: ... and the sgRNA plasmid along with a plasmid containing Cas9 (pLX_311-Cas9, Addgene) were transfected into iPSCs using Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmid containing gRNA targeting AAVSI locus was obtained from Addgene (plasmid #41818). hiPS cells (2 × 106 cells ...
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bioRxiv - Biophysics 2023Quote: DNA was synthesized from a Widom 601 sequence containing plasmid pGEMz_601 (Addgene, 26656) with primers containing a biotin and Cy3 ...
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bioRxiv - Molecular Biology 2023Quote: ... containing the AAVS1-T2 targeting guide and pSH-EFIRES-P-AtAFB2 (#129715, Addgene), which was digested using BglII [#R0144 ...
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bioRxiv - Bioengineering 2023Quote: ... AAV9 vectors containing CMV-driven Cre plasmids were obtained from Addgene (pENN.AAV.CMVs.Pl.Cre.rBG #105537). Vectors were stored in a solution containing PBS and 0.001% Pluronic F-68.
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bioRxiv - Bioengineering 2023Quote: ... a plasmid containing the human codon-optimized Cas12a gene was obtained from Addgene, then was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: A lentiviral plasmid containing a dCAS9/KRAB/MeCP2 cassette was obtained from Addgene. Lenti-X 293T cells were transfected using this plasmid and virus containing the plasmid was generated and appropriate titers were determined ...
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bioRxiv - Genetics 2023Quote: We used the 3XFLAG-VP64-SadCas9-NLS-VP64-containing plasmid (Addgene cat # 135338), in which each sgRNA was annealed and inserted by BsaI directional cloning as described previously31 ...
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bioRxiv - Neuroscience 2023Quote: ... adeno-associated virus containing GCaMP7f (AAV1-hSyn-jGCaMP7f, AddGene viral prep #104488-AAV1) and a second virus encoding the static red indicator mCherry (AAVDJ-hSyn-mCherry ...
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bioRxiv - Cancer Biology 2024Quote: Oligos containing sequences for sgRNAs (Table S5) were cloned into pLentiGuide construct (RRID:Addgene_46911)21 ...
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bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...
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bioRxiv - Neuroscience 2021Quote: ... CRF1:Cre-tdTomato rats were given bilateral microinjections of AAV8-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (50454-AAV8, Addgene, Watertown, MA) or a control virus (AAV5-hSyn-DIO-EGFP ...
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bioRxiv - Neuroscience 2023Quote: ... and female (n=14) rats underwent aseptic stereotaxic surgery for injection of 150nl retrograde-transducing AAV carrying fluorophore mCherry (pAAV-hSyn-mCherry; Addgene; 114472-AAVrg)) into the BLA (AP -3.0 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with plasmids containing the packaging psPAX2 (Addgene, 12260, from Didier Trono) and envelope pCMV-VSV-G (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... The cell line carrying the 5’TOP element-containing SunTag reporter (Addgene plasmid #119946) together with scFv-GFP and NLS-stdMCP-stdHalo was described previously (Wilbertz et al. ...
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bioRxiv - Immunology 2021Quote: ... were subcloned into the lentiviral pLKO.3G vector containing an eGFP cassette (Addgene #14748), by transferring the BamHI-NdeI restriction fragments containing the shRNAs ...
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bioRxiv - Molecular Biology 2022Quote: Separate lentivectors containing spCas9 (lentiCas9-Blast a gift from Feng Zhang (Addgene plasmid # 52962) and sgRNA (lentiGuide-Puro a gift from Feng Zhang (Addgene plasmid # 52963 ...
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bioRxiv - Cancer Biology 2022Quote: ... and lentiviral particles from pLenti6.3/TO/V5 containing IDH1R132H,13 or pLentiCRISPRv2 (Addgene #52961) were produced in 293T cells as previously described18 ...
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bioRxiv - Molecular Biology 2021Quote: ... a DNA fragment containing 5′-AGCCATGGGAAACATCGCAC-3′ was cloned into pL-CRISPR.EFS.tRFP (Addgene#57819) (Heckl et al. ...
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bioRxiv - Genomics 2022Quote: Plasmids containing dCas9-VPR (SP-dCas9-VPR was a gift from George Church (Addgene plasmid # 63798 ...
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bioRxiv - Genomics 2020Quote: SpCas9 gRNAs were cloned into a Tol2-transposon-containing gRNA expression plasmid (Addgene 71485) 59. ...
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bioRxiv - Molecular Biology 2020Quote: ... The Cre-containing plasmid was obtained from Addgene (pLM-CMV-R-Cre, Addgene #27546). A fragment encoding the CMV promoter and mCherry-T2A-Cre-WPRE was excised by NdeI and SacII (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Plasmid containing RelA or p50 was co-transformed with pEVOL-pAzF (Plasmid #31186, Addgene), the plasmid encoding the aaRS/tRNA pair for incorporating the unnatural amino acid p-azidophenylalanine (pAzF ...
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bioRxiv - Microbiology 2021Quote: ... SpyCatcher/Spy-tag and SUMO containing plasmids were purchased from Addgene (#133449 and #111560).
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bioRxiv - Cancer Biology 2021Quote: A549 cells were stably transduced with a lentiviral vector containing H2B-GFP (Addgene #25999) and a lentiviral vector containing DHB-iRFP ...
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bioRxiv - Cell Biology 2022Quote: Microtubules were visualised by expressing a plasmid containing β-tubulin-mCherry (Addgene plasmid #175829).
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bioRxiv - Cell Biology 2022Quote: ... for insertion into a dCas9-EGFP containing backbone (pHAGE-TO-dCas9-3XEGFP (#64107; Addgene)) ...
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bioRxiv - Biochemistry 2019Quote: ... on a 3kb plasmid containing a single 601 sequence (pGEM3Z-601 from Addgene #26656) were performed as previously described (32 ...
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bioRxiv - Neuroscience 2019Quote: ... 600nl of a viral vector containing either pAAV5-hSyn-hM4D(Gi)-mCherry (hM4Di; Addgene viral prep ...
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bioRxiv - Genetics 2020Quote: ... gRNA-containing plasmids were generated in pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene; 62988) (Ran et al ...
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bioRxiv - Biochemistry 2021Quote: ... as a guide RNA (gRNA) and electroporated into HME63 already containing pCas9 (Addgene: 42876). The dnaB gRNA was designed to be centered on the desired mutation site and at the closest adjacent PAM sequence (5’-NGG) ...
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bioRxiv - Immunology 2021Quote: The mammalian expression plasmid containing SARS-CoV-2 HexaPro spike was obtained from Addgene (Addgene plasmid # 154754 ...
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bioRxiv - Immunology 2022Quote: ... a plasmid containing the promoter region of Mus musculus iNos was obtained from Addgene (pGL2-NOS2 Promoter-Luciferase – Plasmid # 19296 from Charles Lowenstein).44 The following primers were designed and used to amplify the promoter for the gene of interest and incorporate XhoI and BamHI sites at the 5’ and 3’ ends ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type MEFs were transduced with lentiviral particles containing the plasmids lentiMPHv2 (Addgene #89308) and lentiSAMv2 (Addgene #75112) ...
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bioRxiv - Cancer Biology 2023Quote: ... Separate lentivectors containing spCas9 (lentiCas9-Blast a gift from Feng Zhang (Addgene plasmid # 52962) and sgRNA (lentiGuide-Puro a gift from Feng Zhang (Addgene plasmid # 52963 ...
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bioRxiv - Immunology 2023Quote: ... pLentiCRISPR-v2 containing guides were transfected wiTHPSPAX2 (Addgene #12260, a gift from Didier Trono) and pMD2.G to generate lentiviral particles ...
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bioRxiv - Bioengineering 2023Quote: ... coli were transformed with plasmid containing the ampicillin resistance gene (pUC19, Addgene Plasmid #50005) and streaked on LB agar plates spiked with 100 µg/mL ampicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... All plasmids containing the CD19 targeting ABD FMC63 are available from Addgene (#200670-#200681).
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bioRxiv - Cell Biology 2023Quote: ... The CYFIP2-mCherry containing plasmid was a gift from Josef Kittler (Addgene plasmid # 122052). The ECFP-betaPIXa containing plasmid was a gift from Rick Horwitz (addgene #15235) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing scFvs targeting TNFRSF8 or TMPRESS11E were cloned into pSLCAR-CD19-BBz (Addgene #135992) using NEB HiFi DNA assembly ...