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Citations for Addgene :
451 - 500 of 770 citations for Rat Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... pET-3d plasmid containing Chicken Capping Protein α1 and β1 subunits was a gift from John Cooper (Addgene plasmid #13451) The N-terminal domain of mouse CAP1 in pSUMOck4 was described earlier66.
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bioRxiv - Microbiology 2019Quote: ... 2.18 μg of env-defective HIV-1 provirus containing GFP reporter was cotransfected with 0.31 μg pMD2.G VSV G plasmid (Addgene #12259). Simian immunodeficiency virus (SIV)−VLPs containing Vpx were produced by the transfection of 2.18 μg pSIV−Δpsi/Δenv/ΔVif/ΔVpr (Addgene #132928 ...
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bioRxiv - Microbiology 2019Quote: ... Simian immunodeficiency virus (SIV)−VLPs containing Vpx were produced by the transfection of 2.18 μg pSIV−Δpsi/Δenv/ΔVif/ΔVpr (Addgene #132928) and 0.31 μg pMD2.G plasmid ...
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bioRxiv - Evolutionary Biology 2020Quote: ... five nanoliters of a solution containing both sgRNAs at a concentration of 40 ng/μL and Cas9 protein (Addgene #47327) at a concentration of 250 ng/μL32 was injected into one-cell-stage medaka embryos ...
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bioRxiv - Cell Biology 2022Quote: - AoSMC Flag/Flag-progerin: Retroviral particles containing pLPC-Flag and PLPC-Flag-progerin which were s a gift from Gerardo Ferbeyre (Addgene plasmid # 69059 ...
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bioRxiv - Cell Biology 2022Quote: - AoSMC Inducible progerin-expressing cells: Lentiviral particles containing pLenti-CMV-TRE3GNeo-GFP progerin (gift from Tom Misteli (Addgene plasmid # 118710), pLentiCMV-TRE3G-GFP-laminA (gift from Tom Misteli (Addgene plasmid # 118709)) ...
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bioRxiv - Cell Biology 2022Quote: ... a fragment containing the GIGYF2 sequence was obtained by PCR from the pcDNA4/TO/GFP-GIGYF2 vector (Addgene plasmid 141189) (34 ...
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bioRxiv - Cell Biology 2022Quote: ... was generated by synthesizing (Genewiz) a sequence containing three copies of mCherry (based on the sequence from pHAGE-EFS-N22p-3XRFPnls; plasmid #75387; Addgene); the sequences encoding the mAID and SMASh degrons (from38) ...
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bioRxiv - Synthetic Biology 2019Quote: We first obtained the yeast strain containing the reporter gene by integrating (lexA-box)x-PminCYC1-Citrine-TCYC1 plasmids (x = 4, 2 or 1) (Addgene plasmids #58434 ...
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bioRxiv - Neuroscience 2019Quote: A lentivirus that was developed for previous work in zebra finches (containing the vector pHAGE-RSV-GCaMP6f; Addgene plasmid #80315) was also used in canaries49 ...
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bioRxiv - Molecular Biology 2019Quote: ... gRNAs were obtained by annealing and cloning complementary primers into vector pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) containing humanized S.pyogenes Cas9n(D10A) nickase (Addgene, cat. # 42335). Primers were designed to target the exon 2 of C6orf203 gene using E-CRISP double nickase platform (http://www.e-crisp.org/E-CRISP/ ...
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bioRxiv - Biochemistry 2021Quote: ... the lentiCRISPRv2 plasmid containing the gRNA was combined with packaging vectors psPAX2 (a gift from Didier Trono, Addgene plasmid # 12260) and pMD2.G (a gift from Didier Trono Addgene plasmid # 12259 ...
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bioRxiv - Neuroscience 2020Quote: ... The constructs were subcloned using restriction digestion into the pHL-sec vector containing a C-terminal 6xHis-tag (Addgene # 99845). Restriction sites for subcloning were introduced by PCR at the 5’ and 3’ ends of scFv-Clasp heavy and light chains (light chain ...
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bioRxiv - Cancer Biology 2020Quote: HEK-293T cells were transfected with the human PSD4 containing pLV lentivirus (VB160428-1095xdp – Vector Builder) together with the 3rd generation lentiviral packaging plasmid (Addgene): pMDLG/pRRE (Gag and Pol) ...
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bioRxiv - Cell Biology 2021Quote: ... and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp) (#17553, Addgene). Forty-eight hours after transfection ...
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bioRxiv - Synthetic Biology 2021Quote: ... S2060 bacteria were transformed with a luciferase reporter containing a quadruplet codon at residue 357 (for example, https://benchling.com/s/seq-7CsWcP8Ez4JNjNM9W23N, Addgene #134787) and an inducible qtRNA expression plasmid (for example ...
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bioRxiv - Biochemistry 2021Quote: ... bacterial cells were co-transformed with the SDC-containing plasmid and pULTRA-CNF (Addgene # 48215, a gift from Peter Schultz) [14] ...
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bioRxiv - Cell Biology 2021Quote: ... Oligos were ordered and BsmBI batch cloned into the gRNA expression cassette of a LentiCRISPR_v2 plasmid containing Cas9 (Addgene 52961). The library was amplified in Stbl3 (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the fragments of 5’ and 3’ homology arms to target AAVS1 locus were subcloned into hOCT4 promoter containing phOCT4-EGFP plasmid (#38776, Addgene) using AAVS1 SA-2A-puro-pA donor plasmid (#22075 ...
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bioRxiv - Cancer Biology 2020Quote: Plasmid pHAGE NFkB-TA-LUC-UBC-GFP-W containing the luciferase gene under the minimal NF-κB promoter was a gift from Darrell Kotton (Addgene plasmid #49343 ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 cyclinB1-Venus/tubulin-mRFP cell line was generated in our lab by transduction with lentiviral vectors containing tubulin-mRFP (Addgene). RPE-1 TUB-mRFP cells were generated in our lab by transduction with lentiviral vectors containing tubulin m-RFP (Addgene) ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 TUB-mRFP cells were generated in our lab by transduction with lentiviral vectors containing tubulin m-RFP (Addgene). HEK293T cells at a 50-70% confluence were co-transfected with lentiviral packaging vectors (16.6 μg of Pax2 ...
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bioRxiv - Molecular Biology 2022Quote: ... hDfs were transduced using lentiviral particles containing the pABpuro-BluF vector (pABpuro-BluF was a kind gift from Steven Brown (Addgene plasmid #46824 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were infected at low viral titer with the pooled mouse CRISPR lentiviral library containing 78,637 gRNAs targeting 19,674 genes (Addgene #73633-LV). Infected cells were selected with puromycin (1 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: The pET28a plasmid vector containing DNA sequences encoding the PANK3 protein (residues pro12 to Asn368) was purchased from Addgene (25518) and transformed into both E ...
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bioRxiv - Synthetic Biology 2022Quote: ... Saturated cultures were centrifuged and resuspended in infiltration medium as described above except that cultures were diluted to 0.3 OD600nm and Agrobacterium strains were mixed in equal ratio with a strain containing pTRV1 (Addgene #148968). Seed of transgenic N ...
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bioRxiv - Cell Biology 2022Quote: ... with plasmids containing Cas9 and the sgRNAs (the backbone used was plasmid PX459, a gift from Feng Zhang (Addgene #62988)) ...
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bioRxiv - Neuroscience 2021Quote: ... We then inserted NFIA and SOX9 cDNA joined by a T2A sequence into an AAVS1 safe-harbor plasmid containing a dox-inducible cassette (Addgene plasmid no ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified fragments were inserted using KpnI and MluI sites into the pCAG vector backbone containing a zinc-finger binding site upstream of a CAG promoter prepared from pCAG-GPHN.FingR-EGFP-CCR5TC (Addgene #46296)17.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A virus lacking the hM4Di DREADD gene and only containing the green fluorescent tag eGFP (AAV8-CaMKIIa-EGFP, packaged by Addgene) was also infused into OFC in separate groups of animals as a null virus control ...
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bioRxiv - Systems Biology 2019Quote: ... pCW-Cas9 Dox-inducible lentiviral vector and pLX-sgRNA lentiviral vector containing AAVS1-targeting sgRNA were all obtained from Addgene.
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bioRxiv - Genetics 2020Quote: ... The plasmid containing 3XFLAG-dCas9/pTEF1p-tCYC1 was a gift from Hodaka Fujii and obtained via Addgene.org (Addgene plasmid #62190) (Fujita et al. ...
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bioRxiv - Genetics 2019Quote: ... The plasmid containing 3XFLAG-dCas9/pTEF1p-tCYC1 was a gift from Hodaka Fujii and obtained via Addgene.org (Addgene plasmid #62190) [31].
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bioRxiv - Systems Biology 2019Quote: ... the cells were transfected with a mix of 8 µg lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library (Addgene #90294) (Hart et al ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were co-transfected with pLX304 plasmids containing constructs of interest and the packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Genetics 2020Quote: ... The appropriate sgRNA oligonucleotides were individually annealed and cloned in a Cas9 or Cas9Nick containing vector: pSpCas9(BB)-2A-Puro (PX459) (plasmid#48139, Addgene) and pSpCas9n(BB)-2A-Puro (PX461 ...
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bioRxiv - Cell Biology 2021Quote: ... preadipocytes isolated from BAT of Slc25a44ĩiox/ĩiox mice were immortalized by using the SV40 Large T antigen as described previously (Yoneshiro et al., 2019) and subsequently infected with a retrovirus containing either empty vector or Cre (#34565, Addgene), followed by hygromycin selection at a dose of 200 μg/ml.
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bioRxiv - Neuroscience 2021Quote: Recombinant adeno-associated virus (AAV) vector containing a CCL2 expression cassette w as generated by replacing GFP cassette of pAAV-CAG-GFP (Addgene) with the full-length c DNA for CCL2 (OriGene) ...
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bioRxiv - Cancer Biology 2020Quote: ... in a lentiviral backbone containing a puromycin resistance sequence (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro, a gift from Charles Gersbach, Addgene plasmid # 71236 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 x 108 Huh7 cells for each of two replicates were transduced at a MOI of ~0.3 with lentivirus containing the Brunello genome-wide library in lentiCRISPRv2 (Addgene 73179), which contains 77.441 sgRNAs targeting 19.114 genes ...
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bioRxiv - Cell Biology 2021Quote: ... Oligos were ordered and BsmBI batch-cloned into the gRNA expression core of a LentiCRISPR_v2 plasmid containing Cas9 (Addgene 52961). The library was amplified in Stbl3 (Thermo ...
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bioRxiv - Genomics 2021Quote: Toronto human knockout pooled library (TKOv3) containing 71,090 based on a lentiCRISPRv2 backbone was a gift from Jason Moffat (Addgene #90294). The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen ...
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bioRxiv - Molecular Biology 2021Quote: ... 180 bp upstream of the stop codon and 500 bp after the FOXO1 top codon were cloned into pUC19 containing FKBP12F36V-2xHA-P2A-mCherry derived from Addgene #104370 (pAW6214 ...
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bioRxiv - Cancer Biology 2022Quote: ... a central region containing a triple FLAG epitope and P2A-neomycin resistance cassette acquired via restriction endonuclease digestion of pFETCH_Donor (Addgene: 63934); and 3 ...
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bioRxiv - Biochemistry 2022Quote: ... All expression constructs were in the form containing an N-terminal His6 and maltose-binding protein (MBP) tag (Addgene #11517).
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bioRxiv - Immunology 2022Quote: ... 3×106 cells were harvested and added to 100 µl supplemented Nucleofector Solution V containing 0.7 µg CIBN-CAAX (Addgene #79574), 1.1 µg CRY2PHRmcherry2-RASAL3 ...
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bioRxiv - Neuroscience 2023Quote: ... E15 wild-type embryos were unipolar injected into the ventricle using a pulled glass micropipette containing a DNA solution (1 μg/μl CAG-GFP plasmid (11150, Addgene) with 0.03% Fast Green in PBS) ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana plants constitutively expressing Cas9 (Cas9 Benthe 193.22 T5 Homozygous; gratefully received from Dan Voytas) together with a strain containing pTRV1 (Addgene #148968) as previously described (Ellison et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The pGEX2TK-Pak1 (70-117) containing the p21-binding domain (PBD) was a gift from Jonathan Chernoff (Addgene plasmid# 12217). For the purification of ARP3 (ACTR3) ...
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bioRxiv - Biochemistry 2023Quote: Phosphorylated tauC3 was expressed in Sf9 insect cells from a 438B pFastBac vector containing an engineered 6xHis tag and a TEV protease cleavage site (Addgene). Following two rounds of virus amplification ...