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Citations for Addgene :
301 - 350 of 1312 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Separately 187.5 ng of pMD2.G (Addgene #12259), 750 ng of pCMV delta R8.2 (Addgene #12263 ...
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bioRxiv - Microbiology 2021Quote: ... mixed with 600 ng of PsPax2 (Addgene # 12260), 600 ng of the lentiviral transfer vector pLenti_CMV-EGFP-2A-mNeonGreen (Addgene # 171599) ...
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bioRxiv - Biochemistry 2021Quote: ... and 250 ng of pMD2.G (Addgene #12259) using FuGENE 6 per manufacturers protocols ...
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bioRxiv - Cell Biology 2020Quote: ... 500 ng Cas9 expression plasmid (Addgene plasmid #43945), and 200 ng of pMaxGFP via nucleofection (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 ng of pcDNA1-TatHIV (Addgene plasmid #138478) and 500 ng of pMD2.G (VSV-G ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of psPAX2 packing vector (Addgene #12260), 333 ng of the pMD2.G VSV-G expression vector (Addgene #12259) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng of pCMV-Tol2 (Addgene no. 31823) and 50 ng CMV-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... and 500 ng of pMD2.G (Addgene #12259) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... 200 ng pDisplay-gGRAB5HT2m-IRES-mCherryCAAX (Addgene, #208710), 200 ng pcDNA3-5-HT2A-P2A-GCaMP8m ...
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bioRxiv - Synthetic Biology 2024Quote: ... 375 ng of PE4max enzyme plasmid (Addgene #174828), 62.5 ng of crRNA plasmid ...
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bioRxiv - Genomics 2019Quote: ... or a human FIRRE cDNA plasmid (htransgene; Dharmacon BC03858) were each transfected together with the selectable marker pPGK-Puro plasmid (gift from R. Jaenisch; Addgene 11349) into ΔFirreXa cells using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Injection mix consisted of 0.5pmol/ul in vitro transcribed RNA and 2.5ng/ul co-injection marker (pmyo-2::mCherry::unc-54 3’UTR) plasmid pCFJ90 (Addgene, plasmid #19327), dissolved in water ...
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bioRxiv - Microbiology 2020Quote: ... was constructed by cutting out the segment containing the hU6 sgRNA expression cassette and the Puro2aBFP selection marker from pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (gift from Kosuke Yusa, Addgene # 67974) and cloning it into a PiggyBac backbone ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg plasmid/transformation for the gene of interest and 0.5 μg plasmid/transformation of transformation marker (pUbi_GUSplus, Addgene, encoding ß-Glucuronidase) was applied.
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bioRxiv - Immunology 2020Quote: ... and upstream of VH81-X (downstream of VH2-2) (Cut2) were cloned by annealing pairs of oligos into pX459 with a puromycin selection marker (Addgene, #62988) following the standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNA against Thap1(see Table S3 for sgRNA sequence) was cloned into LentiCRISPRv2 with a Neo selection marker (Addgene #98292). Brca2Y3308X mES cells 67 were grown on 0.1% gelatin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: U118-MG astrocytoma cells were transfected with the F-actin marker mCherry-LifeAct-7 plasmid (gift from Michael Davidson, Addgene #54491) using a calcium phosphate precipitation protocol.24 LifeAct-7 is a 17 amino acid peptide that binds to the actin cytoskeleton ...
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bioRxiv - Genetics 2019Quote: ... Plasmid phU6-gRNA-CBh-hCas9 was obtained from Rupesh Amin and Mark Groudine and first modified by insertion of the PGK-puro-pA selectable marker from pPGKpuro (Addgene #11349) 74 to obtain pPuro2-hU6-gRNA-CBh-Cas9 ...
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bioRxiv - Genomics 2020Quote: ... The VP64 cassette was replaced by CTCF sequences to generate dCas9-CTCF and neomycin resistant marker that was taken from pAC95-pmax-dCas9VP160-2A-neo (a gift from Rudolf Jaenisch, Addgene 48227) was inserted ...
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bioRxiv - Immunology 2020Quote: ... diacylglycerol probes (diacylglycerol-binding domain of PKCβll tagged with YFP, DBD-YFP), and PM markers (C1AC1A-YFP, CAAX-mCherry, and Memcerulean) were from Addgene (Cambridge, MA). F-actin sensor F-tractin-GFP was obtained from John Hammer (Yi et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection marker was excised by transient in vitro transfection with pCAG-Cre (a gift from Dr. Connie Cepko – Addgene plasmid 1377556) encoding a Cre recombinase and ES cells were then genotyped by PCR using primers F1 ...
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bioRxiv - Genomics 2023Quote: ... a Neomycin resistance gene and a negative selection marker expressing tagBFP and was derived from pDONOR-tagBFP-PSM-EGFP (Addgene 100603). Flanking the positive selection cassette ...
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bioRxiv - Molecular Biology 2023Quote: ... The bleomycin resistance marker and T2A-mCherry were subcloned into the NheI/EcoRI sites pMCB320 sgRNA lentiviral delivery plasmid (Addgene 89359) using InFusion cloning to generate pDM002-sgRNA-lenti-ZeoR ...
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bioRxiv - Cell Biology 2023Quote: ... and pMRXIB (harboring a blasticidin-resistant marker) (Morita et al., 2018) together with enhanced GFP or mRuby3 (codon-optimized, Addgene #74252). STX17 fragments and their point mutations were generated by a standard PCR method or PCR-mediated site-directed mutagenesis.
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bioRxiv - Synthetic Biology 2020Quote: ... The integration of the reporter was performed by co-transfecting 1000 ng TagRFP reporter (5xTetO-pEF-TagRFP-3xNLS) donor plasmid and 500 ng of each TALEN arm (AAVS1-TALEN-L (Addgene #35431) targeting TGTCCCCTCCACCCCACA and AAVS1-TALEN-R (Addgene #35432 ...
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bioRxiv - Systems Biology 2022Quote: ... into the AAVS1 locus by electroporation of K562 cells with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Synthetic Biology 2022Quote: ... into the AAVS1 locus by electroporation of K562 cells with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfer lentiviral plasmids (2500 ng) were diluted in 200 µl serum-free medium together with 225 ng pCMV-VSV-G (Addgene #8454) and 2250 ng psPAX2 (Addgene #12260 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The well was co-transfected with 250 ng of a level 3 vector containing a construct of interest and 125 ng of BxB1 expression plasmid (Addgene #51271) using JetPrime (VWR) ...
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bioRxiv - Molecular Biology 2024Quote: ... reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate enhancer reporter donor constructs into the AAVS1 locus by electroporation of 1 × 106 K562 cells (or K562 cells selected to have the desired synthetic transcription factor) with 1 ng of reporter donor plasmid and 0.5 ng of each TALEN-L (Addgene no. 35431) and TALEN-R (Addgene no ...
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bioRxiv - Cell Biology 2019Quote: Wild-type or SURF4-deficient HEK293T cells that stably express EPO-eGFP and A1AT-mCherry were transfected with a plasmid expressing ERoxBFP (Addgene: 68126, a gift from Erik Snapp (99)) ...
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bioRxiv - Systems Biology 2022Quote: ... and added undiluted to K562 cells for a final cell concentration of 3-4 x 105 cells/mL for pJT126-based effector recruitment vectors (Addgene #161926) or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... or AAV8-hSyn-DIO-GFP (Addgene, n=8, 4 male, 4 female) were injected bilaterally into the BNST (5 × 1012 titer ...
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bioRxiv - Plant Biology 2021Quote: ... was cloned together with pICH47802-p35S::ER:tdTOM::tNOS selection marker (position 1) in the final plant expression vector pAGM4673 (Addgene, catalog number: 48014). For simultaneous live imaging ...
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bioRxiv - Cell Biology 2019Quote: The cell line expressing a DELE1-mClover transgene line from the AAVS1 locus was generated by transfecting a cXG289 population in which ∼ 50% of cells expressed a DELE1-sgRNA and a BFP marker with pXG289 and TALENS targeting the human AAVS1 locus (AAVS1-TALEN_L/R, Addgene #59025 and #59026). Through FACS sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... a 24-well dish was transfected with 250ng of px33043 containing the RBBP6-targeting guide and 250ng of PCR product containing the dTAG degron preceded by a blasticidin or puromycin selection marker (derived from Addgene plasmid 9179224). Jetprime (Polyplus ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression construct containing the target sequence 5’-GGTTTGGTTGATTTCTGCAC-3’ was obtained using a PCR-fusion technique and the PCR product was injected at 15 ng/μL together with 50 ng/μL pDD162 (eft-3prom::Cas9, a gift from Bob Goldstein, Addgene plasmid #47549). After injection ...
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bioRxiv - Bioengineering 2020Quote: ... 600 ng of BxbI Integrase Expression Vector (Addgene #51271) and 600 ng of each landing pad donor plasmid were co-transfected ...
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bioRxiv - Synthetic Biology 2021Quote: ... The evoAPOBEC1-BE4max-NG DNA from pBT375 (Addgene# 125616) were cloned between the NotI and PmeI sites of the PB-TRE-Cas9 with NotI restriction enzyme site insertion ...
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bioRxiv - Biophysics 2020Quote: ... 6750 ng of psPAX2 packaging plasmid (Addgene plasmid # 12260), and 750 ng of Spike-18aa truncated (Addgene plasmid # 149541 ...
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bioRxiv - Molecular Biology 2022Quote: ... 400 ng of dCas9-KRAB expression vector (Addgene #60954), 170 ng of lentiGuide puro (Addgene #52963 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 2.5 ng/µl Pmyo-2::mCherry (Addgene #19327). Three injected animals were pooled and incubated for 3 days at 20 °C before adding 250 ng/µl of hygromycin per plate ...
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bioRxiv - Molecular Biology 2021Quote: ... 6750 ng of psPAX2 packaging plasmid (Addgene plasmid # 12260), and either 750 ng of Spike-18aa truncated (Addgene plasmid # 149541) ...
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bioRxiv - Systems Biology 2019Quote: ... and 300 ng of pCAG-mTagBFP2 (Addgene, Plasmid #122373) plasmids ...
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bioRxiv - Genetics 2020Quote: ... and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Systems Biology 2021Quote: ... 70 ng of VP12 humanSpCas9-Hf1 plasmid (Addgene 72247), and 175 ng of donor reporter plasmid ...
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bioRxiv - Genomics 2020Quote: ... 200 ng of CRISPR-KRAB-MeCP2 vector (Addgene 110821) and 50 ng of gRNA gblock (IDT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and pCFD5-frame_selector_0,1,2 (Addgene 131152; 400 ng/µl each) into nos-Cas9 (attp40) ...
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bioRxiv - Cell Biology 2023Quote: ... 32 ng total/embryo of pCSKA-xwnt8 (Addgene # 16866) plasmid DNA was injected into the 2 dorsal blastomeres of 4-cell stage embryos ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...