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Citations for Addgene :
551 - 600 of 1312 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: The two sgRNAs were co-injected at 120–150 ng/μl together with homemade 6.5 μM Cas9 protein-produced from the pCS2-nCas9n plasmid (Addgene #47929) as described40 in NEB Cas9 buffer (NEB #B0386A ...
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bioRxiv - Neuroscience 2019Quote: ... Constructs were injected into N2 worms at a concentration of 20-50 ng/μl along with Punc-121::RFP (AddGene) at a concentration of 100 ng/μl as a transgenesis marker to bring the final concentration up to 150 ng/μl ...
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bioRxiv - Microbiology 2023Quote: A549-AT cells were seeded in a 96-well format and were transfected with 0.1 ng of pRL-SV40 (Addgene; #27163) together with either 0.1 μg M50 Super 8x TOPFlash (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing, Addgene plasmid # 50005; RRID: Addgene_50005) and 1 ng egfr fragment to generate crude circular DNA mixture.
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bioRxiv - Developmental Biology 2023Quote: ... expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA) (Addgene) using standard methods (42) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... along with left and right TALENs (pZT-C13-L1: Addgene #62196; pZT-C13-R1: Addgene #62197; 375 ng/well each) targeting the human Citrate Lyase Beta-Like (CLYBL ...
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bioRxiv - Microbiology 2024Quote: ... wells were transfected with 500 ng of RSV Gag-GFP (11) and 125 ng of FLAG-tagged Med26 (a gift from Joan Conaway and Ronald Conaway [Addgene plasmid #15367 ...
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bioRxiv - Cell Biology 2020Quote: ... 1.35 µg/ml pPAX2 (Addgene: 12260) and 0.5/ml µg pMD2.G (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1012 GC/ml (Addgene viral prep # 26969-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1012 GC/ml (Addgene viral prep # 105622-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1012 GC/ml (Addgene viral prep # 20298-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 1.9 × 1012 GC/ml (RRID:Addgene_45580 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1012 GC/ml (Addgene viral prep # 162382-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5 × 1012 GC/ml (Addgene viral prep # 18917-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1012 GC/ml (Addgene viral prep # 27056-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... titer: 5.0 × 1013 GC/ml (Addgene viral prep # 104487-AAV1 ...
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bioRxiv - Neuroscience 2021Quote: ... a single 100 nL injection of AAV2-retro-GFP (Addgene #37825-AAVrg) was administered to agranular RSC in the left hemisphere (AP ...
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bioRxiv - Neuroscience 2023Quote: ... a kind gift from Don Arnold) and pENN.AAV.CamKII 0.4.Cre.SV40 (viral titer/ml: 2.3 x 10^13 GC/ml (Addgene 105558-AAV9) in the ratio 2:1:1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding phiC31 integrase under control of a heat shock promoter (Addgene #26290) [73]) ...
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bioRxiv - Molecular Biology 2020Quote: ... Casper strains were available and 20 ng/μL DNA plasmids encoding mNG-GECO1 under the control of nuclear-localized elavl3/HuC promoter (Addgene: 59530) were injected into two-cell stage embryos of Casper mutant zebrafish33 with 40 ng/μl Tol2 transposase mRNA (26 ...
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bioRxiv - Systems Biology 2022Quote: ... lentiviral transduction using a pHAGE-ERBB2 construct (a gift from Gordon Mills & Kenneth Scott, Addgene plasmid #116734, (Ng et al. 2018)) was performed in the presence of 8 μg/ml polybrene (TR1003 ...
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bioRxiv - Immunology 2019Quote: ... These were packaged into a VSV-G pseudotyped lentiviral vector using HEK 293T cells expressing pMD2.G (2 ng, Addgene #12259), pCMV-DR8.2 (5 ng ...
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bioRxiv - Cancer Biology 2020Quote: Cells were plated in 24-well plates and transfected with YAP/TAZ luciferase reporter 8XGTIIC-lux plasmid (50 ng/cm2) (Addgene 34615) together with CMV-lacZ (75 ng/cm2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9-mRNA was in vitro transcribed overnight at 20°C from 400-500 ng XbaI-linearized pT3TS-nCas9n (Addgene, plasmid #46757) using the mMessage mMachine T3 Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding ΦC31 integrase under control of a heat shock promoter (Addgene #26290)60) ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 250 ng of plasmids encoding the SARS-CoV-2 S genes delivered by pTwist-SARS-CoV-2 Δ18 D614G (Addgene, 164437) or pTwist-SARS-CoV-2 Δ18 B.1.1.529 (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... and pRN-Luc (50 ng) reporter plasmids in combination with S protein expressing plasmids pTwist-SARS-CoV-2 Δ18 D614G (Addgene, 164437) or pTwist-SARS-CoV-2 Δ18 B.1.1.529 (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 10% FBS and 1% penicillin-streptomycin, and equal amounts of plasmids (250 ng of each: luciferase reporter, actin-GAL4 (Addgene #24344), one dCas9-Rb constructs ...
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bioRxiv - Genetics 2023Quote: We plated 550,000 HEK293T cells on 6-well plates and 24 hours later we transfected the cells with 900 ng psPAX2 packaging vector (Addgene #12260), 360 ng pMD2.g VSV-G envelope vector (Addgene #12259) ...
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bioRxiv - Neuroscience 2021Quote: ... and pCXLE-hOCT3/4-shp53-F (Addgene plasmid #27077; http://n2t.net/addgene:27077;RRID:Addgene_27077) [pCXLE-hUL ...
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bioRxiv - Genetics 2021Quote: We digested the human STARR-seq screening vector (hSTARR-seq_SCP1 vector_blocking 4, Addgene #99319) with both Thermo SgrDI and BshTI (AgeI ...
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bioRxiv - Cell Biology 2020Quote: HA-NFAT1(4-460)-GFP was a gift from Anjana Rao (Addgene plasmid # 11107). Patterned cells expressing NFAT-GFP was imaged using Zeiss LSM 880 ...
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bioRxiv - Plant Biology 2024Quote: ... for Level 1 and 3 and pEven1-4 (pCsA-E, Addgene plasmids # 136067-136070) for Level 2 ...
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bioRxiv - Neuroscience 2022Quote: ... Titer: 1.8×1013 GC/mL (Addgene – diluted ½) or 4.5×1012 GC/mL (VVF) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1 × 1013 copies/mL) (Addgene, Watertown, MA) were injected into the PeF (250 nL/side ...
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bioRxiv - Neuroscience 2022Quote: ... +0.4 ML with diluted (1:3; Addgene) 4 × 0.15 μL of AAV9-Synapsin-jGCaMP7f-WPRE (Addgene) ...
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bioRxiv - Neuroscience 2022Quote: We injected a single 100 nL of AAV2-retro-GFP (Addgene #37825-AAVrg) in the left agranular RSC (AP ...
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bioRxiv - Neuroscience 2023Quote: ... We injected 50-100 nL of AAV (AAV2/1-Syn-jGCaMP8m-WPRE, Addgene #162375 or AAV2/1-Syn--GCaMP6s-WPRE-SV40 ...
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bioRxiv - Neuroscience 2022Quote: ... wildtype mice were injected with 100-200 nl AAV1-Syn-Cre (AAV.hSyn.Cre.WPRE.hGH, Addgene) in the posterior cerebellum (AP -7.2 ...
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bioRxiv - Developmental Biology 2020Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with 475 ng of a respective CRISPRi plasmid together with 25 ng of Sleeping Beauty transposase-encoding vector pCMV(CAT)T7-SB100 (a gift from Zsuzsanna Izsvak; Addgene plasmid #34879) (Mates et al. ...
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bioRxiv - Genetics 2019Quote: ... The pSI-Target-AID-NG plasmid used for the remaining cytosine base editing experiments was a gift from Osamu Nureki (Addgene plasmid # 119861). The pCMV_ABEmax_P2A_GFP ...
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bioRxiv - Biochemistry 2021Quote: ... and 2000 ng total plasmid DNA per dish including the 50-1000 ng FP-tagged encoding plasmids supplemented with an empty plasmid vector (pCAG-FALSE, Addgene plasmid #89689) depending on the aimed fluorescence level [35] ...
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bioRxiv - Microbiology 2021Quote: ... 3 μg of the resulting covalently closed circular DNA was directly co-transfected with 330 ng of pHEF-VSV-G (Addgene Plasmid #22501) into 70% confluent monolayer of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: The ISRE-Luc reporter plasmid (32) (25 ng) was either transfected alone or co-transfected with a plasmid expressing SARS-CoV-2 genes NSP13 (Addgene, cat. 141379) (25 ng) ...
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bioRxiv - Cancer Biology 2023Quote: ... For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing, Addgene plasmid # 50005; RRID: Addgene_50005) and 1 ng egfr fragment to generate crude circular DNA mixture.
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of either SF3B1 sgRNA or Rosa26 sgRNA in pLKO.5 Puro-2A-GFP backbone was co-transfected with either 1ug of NG-ABE8e (Addgene plasmid 13849) or NG-ABEmax (Addgene plasmid 124163) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were sparsely labeled by administration of AAVs (CamKII.Cre, 104vg/ml, Addgene # 105558-AAV9 and CAG.Flex.EGFP, 108vg/ml, Addgene #28304-PHPeB) at DIV 9-10 and processed for experiments 10-11 days later.
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bioRxiv - Neuroscience 2021Quote: ... viral titer of 5.86×1013 particules/ml) and the Cre recombinase (AAV9-hSyn-Cre, from Addgene, 3.3×1013p/ml), diluted at a factor 10 and 100 respectively ...