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Citations for Addgene :
101 - 150 of 1312 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and 83 ng of nicking guide RNA-(ngRNA-) encoding plasmid or 900 ng of NG-ABE-encoding plasmid (NG-ABE8e, Addgene #138491) and 250 ng of sgRNA-encoding plasmid with the following parameters ...
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bioRxiv - Plant Biology 2021Quote: ... It was then assembled with a FAST-Red selectable marker (Addgene: 117499) and the sgRNA level 1 cassette into a level 2 vector pICSL4723 ...
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bioRxiv - Genetics 2020Quote: ... The 3xP3-DsRed marker gene from the pScarlessHD-DsRed plasmid (Addgene #64703) was PCR amplified ...
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bioRxiv - Developmental Biology 2023Quote: ... the 24xMS2 loop cassette and a DsRed marker (from pHD-DsRed, RRID:Addgene_51434) inserted using a ClaI site for mew and AccII site for sog ...
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bioRxiv - Neuroscience 2021Quote: ... 100-200 nL of rAAV2-retro jGCaMP7s (~1e13 gc/mL, Addgene) was injected at a rate of 70 nL·min-1 using a Microinject system (World Precision Instruments) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 100 ng/µl of Cas9 mRNA transcribed from the linearized MLM3613 plasmid (Addgene 42251; Dahlem et al., 2012). GFP-positive individuals were crossed to wildtype ...
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bioRxiv - Microbiology 2021Quote: The Plasmid expressing SARS CoV-2 Spike protein (Wuhan isolate) was procured from Addgene with 19 nineteen amino acids deletion at C- terminal that enables efficient lentiviral packaging ...
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bioRxiv - Immunology 2022Quote: ... The SARS-CoV spike expression vector was a gift from David Nemazee (Addgene #170447).
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bioRxiv - Immunology 2021Quote: The mammalian expression plasmid containing SARS-CoV-2 HexaPro spike was obtained from Addgene (Addgene plasmid # 154754 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Neuroscience 2022Quote: ... The stress granule marker GFP-G3BP1 was also obtained from Addgene (#135997 57), while the LC3-mCherry construct was obtained from Dr ...
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bioRxiv - Synthetic Biology 2020Quote: ... the natNT2 marker was PCR amplified from pUG-natNT2 (Addgene plasmid #110922, (26)) using primers 10297 and 10298 ...
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bioRxiv - Cell Biology 2020Quote: ... and plasmid coinjection markers pGH8 (rab-3P∷mCherry∷unc-54utr; plasmid 19359; Addgene), pCFJ104 (myo-3P∷mCherry∷unc-54utr ...
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bioRxiv - Cell Biology 2022Quote: ... Autophagosome-specific marker mCherry-hLC3B-pcDNA3.1 was a gift from David Rubinsztein (Addgene plasmid # 40827 ...
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bioRxiv - Neuroscience 2022Quote: ... a GFP-kanamycin marker was PCR amplified from a template vector (PL452, Addgene). The primers include homology arms consisting of 50 nucleotides upstream and downstream from the start codon of the vav gene ...
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bioRxiv - Genetics 2023Quote: ... an attB sequence and a mini-white marker (backbone originally from Addgene 140620) These constructs were inserted into the attP40 on the second chromosome using standard integrase-mediated transgenesis method.
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid, 500 ng pCMVR8.74 Addgene plasmid # 22036 ...
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bioRxiv - Physiology 2021Quote: ... 200nL AAV-hM3D(G)q-mCherry (Addgene 44361-AAV8, 4×1012 vg/mL) was injected bilaterally at 50nl/min and mice were allowed 2 weeks recovery prior to testing ...
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bioRxiv - Biochemistry 2021Quote: ... 27μg pTT5LnX-WHCoV-St19 (SARS-CoV2 Spike) and 54 μg pHIV-Luc-ZsGreen (Addgene, US) using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: The plasmid with cDNA encoding SARS-Cov-2 spike HexaPro (S) was obtained from Addgene. To express the S protein ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Cell Biology 2021Quote: ... and pCMV3 nCoV2019 Spike (D614 variant) C-FLAG Hygro was obtained from Addgene (Teddington, UK). Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G, Addgene #12259) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... we used an existing CMV-driven SARS-CoV-2 plasmid (pcDNA3.1-SARS2-Spike, Addgene 145032)(Shang et al. ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G (Addgene #12259)) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified Cas9n-NG DNA from pX330-SpCas9-NG (Addgene# 117919), PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847 ...
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bioRxiv - Molecular Biology 2021Quote: ... EF1a promoter driven hSpCas9 with a hygromycin resistance marker (modified version of Addgene #52961) was stably integrated via lentiviral transduction into the previously described TetR-CBX7 reporter mESC cell line ...
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bioRxiv - Neuroscience 2019Quote: ... or GFP (pAAV-CaMKIIa-EGFP; AAV5) as a fluorescent marker (Addgene, Cambridge Massachusetts, US).
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bioRxiv - Neuroscience 2024Quote: ... to express channelrhodopsin (ChR2) or control virus (n = 4, AAV-Ef1a-fDIO-mCherry, 200 mL, 3 × 1012 GC/mL; Serotype: 9; Addgene) was unilaterally injected over 10 minutes into the MePD using a 2-µL Hamilton microsyringe (Esslab ...
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bioRxiv - Bioengineering 2020Quote: ... 1 pmol of the oligo mix was added to a 20 μl Golden Gate reaction containing 100 ng destination plasmid (pATT-DEST, Addgene #79770), 10 units BsaI (NEB #R3733) ...
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bioRxiv - Neuroscience 2022Quote: ... Experiments with inducible Cre used 2-20 fmol (10-100 ng) pCAG ERT2-Cre-ERT2 (Addgene #3777, Matsuda and Cepko, 2007) per coverslip ...
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bioRxiv - Developmental Biology 2021Quote: ... The injection mix was prepared in MilliQ H2O and contained 60 ng/mL Peft-3::Cas9 (Addgene #46168), 45 ng/mL each sgRNA plasmid ...
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bioRxiv - Genomics 2022Quote: ... Per transfection reaction we used 7.5ul of 1 mg/mL polyethyleneimine (PEI) and 2.325 μg of total plasmid DNA (825 ng psPAX2: Addgene #12260 ...
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bioRxiv - Developmental Biology 2021Quote: ... Injection mixes were prepared in MilliQ H2O and contained 50 ng/ml Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1-phSyn1(S)-FLEX-TdTomato-T2A-Syp-EGFP (Addgene #51509, 4 x 1014GC/mL) was injected (60nL at 20nL/min ...
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bioRxiv - Systems Biology 2019Quote: ... 1000 ng of lentiCRISPRv2_sgMS2_1 and 300 ng pCAG-mTagBFP2 (Addgene, Plasmid #122373) plasmids were transfected using Lipofectamine 3000 into each cell line ...
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bioRxiv - Immunology 2021Quote: ... with pcDNa3.1 vector expressing SARS-CoV-2 spike (BEI repository) and the helper plasmid pSPAX2 (Addgene). The VSV-G and empty lentiviruses were produced by replacing pCDNA3.1-Spike with pcDNA3.1-VSV-G or pCDNA3.1 empty vector ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1-SARS2-Spike (2) and pcDNA3.1-hACE2 (2) were obtained from Addgene (Addgene plasmids #145032; #145033); pCI-SARS2-Spike expressing a codon optimized version of the gene coding for S protein of SARS-CoV-2 was provided by Nicolas Escriou (Institut Pasteur) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 whole spike protein HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754). SARS-CoV-2 HexaPro was expressed in Expi293F cells and purified with Ni-NTA resin followed by size exclusion as described previously [61] ...
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bioRxiv - Immunology 2021Quote: ... NG-ABEmax (35) (Addgene plasmid #112095 ...
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bioRxiv - Immunology 2022Quote: ... 750 ng pSPAX2 (Addgene), 250 ng VSVG ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transfected with intracellular markers for the plasma membrane (LCK-GFP; Addgene plasmid #61099), endoplasmic reticulum (pLV-ER GFP ...
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bioRxiv - Systems Biology 2021Quote: We exchanged the antibiotic selectable marker on the lentiviral expression vector H2B-mRuby2 (Addgene #90236) from hygromycin to puromycin ...
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bioRxiv - Biochemistry 2022Quote: ... Expression constructs for various cytoskeleton or organelle marker proteins were obtained from Addgene (S1 Table). For bacterial expression of His6-tagged CAHS proteins ...
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bioRxiv - Cell Biology 2020Quote: ... pCFJ90 and pCFJ104 served as co-injection markers and were gifts from Erik Jorgensen (Addgene plasmid # 19359 ...
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bioRxiv - Systems Biology 2023Quote: ... upstream of a T2A-mCherry-BSD marker using GoldenGate cloning into backbones pJT126 (Addgene #161926). For validations ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells plated on polylysine-coated coverslips were transfected with cDNA (100 ng/40000 cells) coding for mitochondrial-targeted mCherry (mCherry-Mito-7, a gift from Michael Davidson (Addgene plasmid # 55102), (Olenych et al ...
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bioRxiv - Genetics 2019Quote: ... 600 ng of the mutant SCN5A plasmid and 100 ng of a plasmid expressing Bxb1 recombinase (a gift from Pawel Pelczar,21 Addgene plasmid #51271). On day 1 ...
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bioRxiv - Genomics 2022Quote: sgRNA targeting the genomic region of integration was cloned in BbsI linearized pX335-U6-Chimeric_BB-CBh-hSpCas9n (99) (D10A) plasmid (Addgene #42335) using NEBuilder HiFi DNA Assembly Master Mix (1:3 molar ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV2-CAG-TdTomato (100 µL, 5.3 x 1012 GC/ml, Addgene 59462-AAV2) was injected intravitreally 4 mm posterior to the nasal aspect of the limbus ...