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Citations for Addgene :
151 - 200 of 1312 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and AAV2-CAG-TdTomato (100 µL, 5.3 x 1012 GC/ml, Addgene 59462-AAV2) was injected intravitreally 4 mm posterior to the nasal aspect of the limbus ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV5-hSyn-dLigh1.2 (titer ≥ 4×1012 vg/ml) was a gift from Lin Tian (Addgene viral prep #111068-AAV5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.5 μg of SARS-CoV-2 Spike plasmid (a gift from Fang Li, Addgene plasmid #145032) using Lipofectamine 3000 transfection reagent (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-SCoV-2S (Spike envelope plasmid; Sinobiologicals – Cat #: VG40589-UT) and pLJM1-EGFP (reporter; Addgene plasmid #19319) in a ratio of 1:1:2 respectively ...
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bioRxiv - Genetics 2020Quote: ... we co-transfected pcDNA3.1-SARS2-Spike or pcDNA3.1-SARS2-SpikeD614G (see above) with pcDNA3.1-ACE2 (Addgene 1786) at a 1:1 ratio (Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032);34 this gene was cloned into a modified pcDNA 3.1 backbone (Clontech-Takara ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032).
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV S was cloned into pcDNA3.3 vector from pcDNA3.1-SARS-Spike (Addgene plasmid#145031). SARS-CoV-2 N was cloned into a pSGC-his vector as described in our previous paper [17] ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids encoding the spike glycoprotein of vesicular stomatitis virus (VSV-G) and Cas9 were obtained from Addgene (cat ...
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bioRxiv - Synthetic Biology 2020Quote: ... GEM effector expression cassette together with the LEU2 marker was PCR amplified from pHES83917 (Addgene # 87941) using primers OZL348 and OZL349 and integrated downstream of the YFL033C locus of SZL149 and SZL281 (SZL149+msh2Δ) ...
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bioRxiv - Cancer Biology 2023Quote: ... Each mutant was then inserted into a lentiviral vector with a puromycin resistance marker (Addgene #17448).
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bioRxiv - Developmental Biology 2023Quote: ... 100K-200K HEK 293 cells were reverse transfected with the M50 Super 8x TOPFlash plasmid (100 ng; Addgene #12456; gift from Randall Moon) and a construct constitutively expressing Renilla luciferase (5 ng ...
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bioRxiv - Bioengineering 2021Quote: ... with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431) and TALEN-R (Addgene #35432 ...
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bioRxiv - Genomics 2022Quote: ... The SpCas9-NG ORF was subcloned from pX330-SpCas9-NG obtained from Addgene (#117919), and assembled with TEF1 promoter and CYC1 terminator (Zhang et al. ...
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bioRxiv - Genomics 2020Quote: ... and NG-ABEmax (Addgene #124163) were gifts from David Liu ...
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bioRxiv - Microbiology 2022Quote: ... 400 ng psPAX2 (Addgene 12260), and 500 ng plentiguide puro expression vector (Addgene cat # 52963) ...
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bioRxiv - Cell Biology 2021Quote: ... psPAX2 (Addgene, 12260, 1,250 ng), and the sgRNA containing vector (e.g. ...
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bioRxiv - Genomics 2021Quote: ... psPAX2 (Addgene 12260, 1250 ng)62 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2250 ng psPAX2 (Addgene 12260) and 225 ng VSV-G (Addgene 8454 ...
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bioRxiv - Biochemistry 2021Quote: ... 750 ng psPAX2 (Addgene #12260), and 250 ng of pMD2.G (Addgene #12259 ...
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bioRxiv - Genetics 2020Quote: ... psPAX2 (Addgene 12260, 1250 ng), and the transfer vector (e.g. ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng psPAX2 (Addgene #12260), 50 ng pMD2.G (Addgene #12259 ...
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bioRxiv - Genomics 2023Quote: ... psPAX2 (Addgene 12260, 1250 ng), and the transfer vector (e.g. ...
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bioRxiv - Biochemistry 2023Quote: ... 900 ng psPAX (Addgene, #12260), and 100 ng pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... 600 ng psPAX2 (Addgene, 12260), and 300 ng pMD2 (Addgene ...
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bioRxiv - Genomics 2023Quote: ... psPAX2 (Addgene 12260, 1250 ng)63 ...
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bioRxiv - Genomics 2024Quote: ... 1,750 ng psPax2 (Addgene #12260), and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega ...
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bioRxiv - Genomics 2024Quote: ... 600 ng psPAX2 (Addgene #12260) and 500 ng of the transfer vector ...
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bioRxiv - Neuroscience 2021Quote: AAV5.CaMKII.hChR2 (H134R)-mCherry (100 μL at titer ≥ 1 × 10 13 vg/mL) from Addgene was injected in 4-week-old rats intracerebrally in the right somatosensory forepaw region ...
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bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032) 39 ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenised and barcoded spike sequence templates were then added into MluI and XbaI digested lentivirus backbone (Addgene #204579) using HiFi reaction (NEB E2621L) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... donor vector (400 ng µl-1) and pHsp70-Cas9 (400 ng µl-1) (Addgene #45945) [69] ...
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bioRxiv - Neuroscience 2019Quote: ... donor vector (400 ng µl−1) and pHsp70-Cas9 (400 ng µl−1) (Addgene #45945)68 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified amplicons were mixed (30 ng each) with 50 ng pJET1.2-STOP-dsRed (51) (Addgene plasmid # 60944 ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Plant Biology 2019Quote: ... The pB_35S/mEGFP plasmid was prepared by cloning the mEGFP coding sequence directly into the pB2GW7 backbone, replacing the ccdB counterselectable marker (www.addgene.org, plasmid #135320 (RRID:Addgene_135320)) ...
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bioRxiv - Genetics 2020Quote: gRNA-marker vectors were constructed similarly to pAC-U63-tgRNA-Rev (Addgene 112811, Poe et al., 2019) but have either a ubi-nBFP (in pAC-U63-tgRNA-nlsBFP ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Selectable markers (conferring kanamycin resistance and streptomycin sensitivity) were cloned from pSK+KanaRpsL [68] (AddGene plasmid #20871) and flanked with upstream GAL4 and downstream HSP70-UTR arms cloned from pBPGUw [69] ...
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bioRxiv - Cell Biology 2020Quote: ... pCFJ90 and pCFJ104 served as co-injection markers and were gifts from Erik Jorgensen (Addgene plasmid # 19359; http://n2t.net/addgene:19359; RRID:Addgene_19359, Addgene plasmid # 19327 ...
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bioRxiv - Plant Biology 2022Quote: ... The plasma-membrane marker construct MpEF1a:myr-mScarlet was generated by LR recombination into pMpGWB303 (Addgene no. 68631)68 ...
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bioRxiv - Cancer Biology 2024Quote: HEK-293T cell line was transduced with a Cas9 lentiviral vector with blasticidin selection marker (Addgene# 52962) and selected with blasticidin (8 ug/ml) ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... followed by a GSG linker and mouse IgG2a Fc fusion (amino acid 99-330; UniProtKB/Swiss-Prot P01863) were synthesized and then cloned into pENTR (Addgene Plasmid #17398) using InFusion cloning according to manufacturer’s instructions (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA encoding CGI-99 (Uniprot Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (Uniprot Q52LJ0 ...
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bioRxiv - Biochemistry 2021Quote: DNA encoding amino acids Phe2-Asp101 of human CGI-99 was cloned into the UC Berkeley MacroLab 1B vector (Addgene plasmid # 29653). The fusion protein containing an N-terminal His6 tag and a TEV protease cleavage site was expressed in BL21 (DE3 ...
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bioRxiv - Genetics 2021Quote: ... 2 ml (1.5× 106/ml) cells plated in a well of a 6-well dish were transfected with 300 ng of Act::Cas9 (Addgene #62209) and the respective gRNA and repair templates for the 25C6 (75 ng pU6-3-25C6-gRNA1 (DGRC Cat# 1547) ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 ng reference reporter (pcDNA3.1-Nanoluc-3xFLAG-V5) and 50 ng 3xFLAG tagged constructs (Addgene #87063). Transfection was carried out using Lipofectamine 3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... 10 ng/μl of the appropriate landing pad plasmid and 2.5 ng/μl pCFJ421 (Addgene #34876) (Frøkjær-Jensen et al ...
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bioRxiv - Neuroscience 2023Quote: Viral vectors encoding the fluorescent dopamine indicator dLight1.2 (AAV5-hSyn-dLight1.2) (Addgene, titer ≥ 4×10¹² vg/mL) and calcium indicator jRCaMP1b (AAV1.Syn.NES-jRCaMP1b.WPRE.SV40 ...