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Citations for Addgene :
2051 - 2100 of 10000+ citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... TbC1 cells were reverse transfected with 400ng of U6>sgRNA plasmids (George Church, Addgene plasmid #41824) according to Table S2 and 3μL Lipofectamin 2000 (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid HIV-dual-GT including the final sequence is available from AddGene (plasmid ID 122696). Individual CRISPR knock-out plasmids were constructed based on lentiCRISPRv2 (#52961 ...
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bioRxiv - Genomics 2020Quote: ... Cells were transfected with 0.5 ug gRNA gblock and 2.5 ug px458 plasmid (Addgene plasmid # 48138) containing spCas9 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... Flag pLJM1 RagB and RagD plasmids were gifts from David Sabatini (Addgene plasmid 19313 and 19316) (Sancak et al. ...
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bioRxiv - Neuroscience 2019Quote: ... and Cas9 mRNA was transcribed in vitro from linearized template plasmid pMLM 3613 (Addgene Plasmid #42251). A mixture containing approximately 300ng/μl Cas9 mRNA and 20ng/μl sgRNA was injected into fertilized eggs at the one-cell stage ...
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bioRxiv - Plant Biology 2019Quote: ... We have also made available a hygromycin-selectable mEGFP plasmid, pH_35S/mEGFP (www.addgene.org, plasmid #135321 (RRID:Addgene_135321)) ...
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bioRxiv - Cancer Biology 2020Quote: ... This plasmid was a gift from Kai Ge (Addgene plasmid #15548; http://n2t.net/addgene: 15548; RRID:Addgene_15548) (70).
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bioRxiv - Cancer Biology 2020Quote: ... This plasmid was a gift from Feng Zhang (Addgene plasmid # 52962; http://n2t.net/addgene:52962; RRID:Addgene_52962) (69).
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bioRxiv - Bioengineering 2021Quote: ... with the following plasmids: lenti-EF1a-dCas9-VPR-Puro plasmid (gift from Kristen Brennand; Addgene, #99373) or the sgRNA expression cassette lentiGuide-Puro (gift from Feng Zhang ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected with transgene plasmid together with lentivirus packaging plasmids psPAX2 (Addgene, Cat. No. 12260) and pMD2.G (AddGene ...
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bioRxiv - Cancer Biology 2021Quote: Lentivirus was generated by transfecting the target plasmid with the packaging plasmids pMD2.G (Addgene: 12259) and psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... List of plasmids used in this work: pCDH-puro-Bcl2 (Cheng et al., Addgene plasmid #46971), Tet-pLKO-puro (Wiederschain et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... the gag/pol plasmid pCMV-dr8.91 and the VSV-g envelope plasmid pMD2.G (Addgene, 12259). The plasmids were diluted in H2O and 2.5 M CaCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain was transformed with the pBAD::mRFP1 plasmid (Addgene plasmid #54667; Campbell et al., 2002) or the pZsGreen vector (cat ...
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bioRxiv - Neuroscience 2022Quote: Ankyrin-G-190-GFP (plasmid #31059) and Ankyrin-B-2XHA (plasmid #31057) were bought from Addgene. The 3XHA-Ankyrin-G domain constructs were gifts from Dr ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid pMK1334 for lentiviral delivery of sgRNA was a gift from Martin Kampmann (Addgene plasmid #127965). Sequences in transfer vectors were incorporated into lentivirus using the lentiviral packaging system (pMD2.G and psPAX2) ...
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bioRxiv - Microbiology 2022Quote: ... We introduced this sgRNA-encoding sequence into the pSAG1::Cas9-U6::sgUPRT plasmid (Addgene plasmid 54467) using Q5 mutagenesis (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid pCDNA-3xHA-hTERT was obtained from (Addgene plasmid # 51637 ; http://n2t.net/addgene:51637 ; RRID:Addgene_51637) [27] ...
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bioRxiv - Neuroscience 2020Quote: ... Lentiviral packaging was performed in HEK 293T cells using commercial plasmids (Addgene plasmids 12259 and 12263) and protocols ...
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bioRxiv - Microbiology 2021Quote: ... psPAX2 and pMD2.G were a gift from Didier Trono (Addgene plasmid # 12260, Addgene plasmid # 12259) and pLenti CMV GFP Neo (657-2 ...
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bioRxiv - Cancer Biology 2020Quote: The pKLV-U6gRNA-PGKpuro2ABFP (Plasmid #50946) and the lentiCas9-Blast (Plasmid #52962) were obtained from Addgene. The HSV1-tk/GFP/firefly luciferase (TGL ...
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bioRxiv - Biochemistry 2021Quote: ... The pcDNA3.1-SC35-cMyc SRSF2 plasmid was a gift from Kathleen Scotto (83) (Addgene plasmid #44721).
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bioRxiv - Genetics 2022Quote: ... Complementary gRNA oligonucleotides were cloned into pSpCas9(BB)-2A-Puro plasmid (pX459; Addgene plasmid ID# 48139) using the BbsI I site ...
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bioRxiv - Systems Biology 2022Quote: ... pSNAPf-Cox8A Control Plasmid was a gift from New England Biolabs & Ana Egana (Addgene plasmid # 101129). The primers used for cloning were synthesized by BioTeZ Berlin-Buch GmbH and are listed in Table S3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... the 4272 bp ORF of Cas9 was amplified from pHsp70 Cas9 plasmid (Addgene plasmid #46294 (32)) using primers BR-666 and BR-667 and cloned into the vector between the zpg promoter and the 3’-UTR for zpg using BbsI for scarless ...
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bioRxiv - Microbiology 2022Quote: The RNA motif plasmid cloning backbone [(pRMB) (Addgene plasmid # 107253; http://n2t.net/addgene:107253; RRID: Addgene_107253)] and the BASU RaPID plasmid (Addgene plasmid # 107250 ...
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bioRxiv - Cancer Biology 2022Quote: Plasmid pDONR221-SLC3A2_STOP was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 161379, RRID:Addgene_161379) [40] ...
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bioRxiv - Cancer Biology 2022Quote: Plasmid pDONR221-SLC3A2_STOP was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 161379, RRID:Addgene_161379) [40] ...
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bioRxiv - Cell Biology 2022Quote: ... LentiCas9-Blast plasmid (a gift of Feng Zhang, Addgene plasmid # 52962; http://n2t.net/addgene:52962; RRID:Addgene_52962) was used to produce virus in 293T cells which was used to infect RPE-1 and RPE-1-21/3 cell lines ...
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bioRxiv - Cancer Biology 2019Quote: ... A plasmid for over expression of FGFR1 was a gift from Dominic Esposito (Addgene plasmid #70367) and cloned into an expression vector (pHIV-IRES-mRFP ...
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bioRxiv - Cancer Biology 2020Quote: ... Blasticidin-resistant cells were subsequently transduced with viral supernatant containing LentiGuide-Puro plasmid (Addgene plasmid #52963) with ligated selected sgRNA ...
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bioRxiv - Pathology 2021Quote: The constitutively activated STAT3 plasmids (STAT3-C Flag pRc/CMV) were purchased from Addgene (Plasmid #8722). C2C12 cells were transiently transfected with STAT3-C plasmids using the Lipofectamine™ 2000 Transfection Reagent according to the manufacturer’s instructions (Life Technology ...
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bioRxiv - Genetics 2019Quote: ... the DNMT3B2 sequence from pcDNA3/Myc-DNMT3B2 plasmid (Addgene plasmid 36942, a gift from A. Riggs)27 was subcloned using XbaI and BamHI into the pCG plasmid (a gift from N ...
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bioRxiv - Microbiology 2020Quote: ... The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li ...
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bioRxiv - Genetics 2020Quote: ... The empty sgRNA plasmid backbone was a kind gift from Keith Joung (BPK1520, Addgene plasmid #65777). The SpCas9 expressing vector was created by using Q5 high fidelity polymerase (NEB ...
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bioRxiv - Cell Biology 2021Quote: Early passage HeLa-M cells were transfected with 1.5 µg of px459 plasmid (Addgene plasmid #62988) containing a single guide RNA (sgRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... or pSRE-Luciferase plasmid from ATCC (# MBA-120) together with packaging plasmids pMD2.G (Addgene, #12259), psPAX ...
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bioRxiv - Synthetic Biology 2021Quote: ... pInt plasmid was a gift from Michele Calos (Addgene plasmid # 18941 ; http://n2t.net/addgene:18941 ; RRID:Addgene_18941) and pTcI-Wasabi was a gift from Mikhail Shapiro (Addgene plasmid # 86101 ...
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bioRxiv - Biophysics 2021Quote: ... The pMal-Abl-PRM5R plasmid was a generous gift from Michael Rosen’s Lab (Addgene plasmid #112088). The sequence encoding 7x repeats of a PRM from lamellipodin (aa 970-981 ...
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bioRxiv - Neuroscience 2021Quote: ... APEX2 was amplified from the pcDNA3 APEX2-NES plasmid (gift from Alice Ting, Addgene plasmid #49386) with three sequential sets of primers to add SV40 NLS to both the N-terminus and C-terminus of APEX2 (primer sets 1-3 ...
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bioRxiv - Cancer Biology 2020Quote: MTR knockout using CRISPR-Cas9 was accomplished using the pLenti-CRISPR v2 plasmid (Addgene Plasmid 49535)58 ...
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bioRxiv - Biophysics 2021Quote: ... BL21 AI cell was co-transformed with MutS-bio and BirA expression plasmid (Addgene plasmid #20857)70 and was grown in LB containing 50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2021Quote: ... two constructs (Fig. 1A) were made using a modified pgRNA-bacteria plasmid (NEB, Addgene plasmid # 44251). This plasmid ...
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bioRxiv - Immunology 2020Quote: ... LeGO-iT2 and LeGO-iT2puro plasmids were gifts from Boris Fehse (Addgene plasmids #25917 and #27343). pcDNA3.1+_C-(K)DYK-ACE2 (NM_021804.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells were transfected with lentiviral plasmid and the second-generation packaging plasmids psPAX2 (Addgene, #12260) and pMD2.G (Addgene ...
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bioRxiv - Genomics 2022Quote: ... Base editor plasmids pCMV_AncBE4max_P2A_GFP and pCMV_ABEmax_P2A_GFP were gifts from David Liu (Addgene plasmid # 112100 and 112101).34 Base editor constructs pCAG-CBE4max-SpG-P2A-EGFP (RTW4552 ...
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bioRxiv - Immunology 2022Quote: ... LV-TCR plasmids were based on the pRRL backbone derived from pRRLSIN.cPPT.PGK-GFP.WPRE (plasmid #12252, Addgene). The DNA sequences of all plasmids were verified by Sanger sequencing performed by GENEWIZ ...
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bioRxiv - Neuroscience 2022Quote: The GFP RNAi construct was plasmid L4417 was a gift from Andrew Fire (Addgene plasmid #1649). Bacteria were isolated and grown overnight at 37°C shaking in Luria broth (LB ...
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bioRxiv - Microbiology 2022Quote: ... which we copied from the EGFP-GalT plasmid (gift from Jennifer Lippincott-Schwartz, Addgene plasmid # 11929) [56] ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmid version of this construct will be available on Addgene (Addgene Plasmid #174007, RRID:Addgene 174007).