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Citations for Addgene :
1551 - 1600 of 2662 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... IMR90 cells were transfected with 1 µg of pEGFP-Δ50 LMNA (Addgene Plasmid #17653). After expression of the GFP was observed (∼2 days) ...
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bioRxiv - Bioengineering 2020Quote: ... U87-EGFP cells were created by infection with lentivirus made from pLL3.7 (Addgene #11795). The drug screens were performed by the Quellos High Throughput Screening Core (University of Washington ...
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bioRxiv - Neuroscience 2021Quote: ... 1 million cells were nucleofected with iμg pAAV-CAG-GFP plasmid DNA (Addgene 37825) in Ingenio electroporation buffer (Mirus MIR50111 ...
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bioRxiv - Genomics 2019Quote: ... the 70-80% confluent cells were transfected with 780 ng psPAX2 (Addgene plasmid #12260), 510 ng pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus was generated in 293T cells using delta 8.9 and pHCMV-EcoEnv (Addgene 15802) as packaging plasmids and 25kDa linear PEI as a transfection agent ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with lentiviral packaging vectors (3 μg pSPAX2 (Addgene plasmid, 12260) and 1 μg pMD2.G (Addgene plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa cells (ATCCCCL-2) were transiently transfected with SpCas9-expressing PX459 plasmid (Addgene # 62988) using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Parental Cas9 cell lines were generated by viral infection with lentiCas9-Blast (Addgene, # 52962) followed by blasticidin selection for 7 days ...
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bioRxiv - Cell Biology 2022Quote: For transient cell transfections the following plasmids were used: talin-mCherry (Addgene plasmid # 55137), vinculin-mCherry14 ...
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bioRxiv - Cell Biology 2022Quote: ... RAW264.7 cells seeded onto coverslips were transfected with GCpGP-CMV-GCaMP6s (Addgene plasmid 40753) using FuGENE HD (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral transfer vectors were transfected into 293T cells with packaging vector pspax2 (Addgene, 12260) and envelop vector vsvg using linear polyethylenimine ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (∼107) were transiently transfected with either pRK5-MYC-mTOR (Addgene plasmid #1861) or pRK5-MYC-mTOR kinase-dead (Addgene plasmid #8482) ...
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bioRxiv - Biochemistry 2023Quote: ... and TEV protease cleavage site into an insect cell expression vector (Addgene plasmid #55218). The same expression construct was cloned into a bacterial expression vector (Addgene plasmid #29708) ...
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bioRxiv - Cancer Biology 2023Quote: ... KMS11 or JJN3 cells were transduced with the pCW-Cas9-Blast vector (#83481, Addgene) to establish stable Cas9+KMS11 and Cas9+JJN3 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA from RPE1 cells was cloned into pLenti CMVie-IRES-BlastR (Addgene plasmid #119863). PAXIP1 W75R ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 0.24μg EGFP-LC3 plasmid DNA (Addgene #11546; Watertown, MA, USA) using the FUGENE6 transfection reagent (Promega ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with paxillin-pEGFP (a gift from Rick Horwitz; Addgene plasmid #15233) using a microporator (Neon Transfection System ...
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bioRxiv - Cancer Biology 2023Quote: ... and PEI transfected into HEK293T cells along with pUMVC retroviral packaging construct (Addgene # 8449) and VSVG envelope construct (Addgene #8454) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... carbenicillin/ampicillin resistance was introduced into NA22 competent cells using pUC1975 (Addgene, product #50005) to generate strain PXKR1 ...
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: One population of U-87 MG cells were transiently transfected with pLV-mitoDsRed (Addgene #44386 ...
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bioRxiv - Genomics 2023Quote: K562 CRISPRi cells were transduced with the hCRISPRi-v2 top 5 library (Addgene #83969)138 with 840X representation ...
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bioRxiv - Genetics 2023Quote: ... 293T cells were transfected with the plasmids pCMVR8.74 (a gift from Didier Trono (Addgene plasmid #22036 ...
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bioRxiv - Neuroscience 2023Quote: ... At DIV0 cells were infected with either AAV-Cre-GFP-hSyn (Addgene, 105540-AAV9) or AAV-GFP (Addgene ...
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bioRxiv - Cell Biology 2023Quote: The producer cell line HEK 293TN was transfected with the packaging (psPAX2, Addgene 12260), envelope (pMD2.G ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h cells were co-transfected with 1.3 pmol of psPAX2 (Addgene, 12260), 0.72 pmol pCMV-VSV-G (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: MiaPaCa2 cells were transfected with the bicistronic pcDNA3-RLUC-POLIRES-FLUC plasmid (Addgene 45642) (55 ...
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bioRxiv - Cancer Biology 2023Quote: ... GBM cells (G411) were transduced with lentiviral vector pBMN (CMV-copGFP-Luc2-Puro, Addgene plasmid #80389 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with pFlagCMV2-14-3-3sigma (Addgene, Cambridge, MA, Plasmid #12453) (68) ...
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bioRxiv - Cell Biology 2023Quote: ... a stable cell line was created using pLV-Stargazin-mTurquoise2-iLID (Addgene Plasmid #161001) (54 ...
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bioRxiv - Cancer Biology 2023Quote: VUMC-ATRT-01 cells were transduced (lentiviral) with a LentiCRISPR v2 plasmid (#52961, Addgene) that was cloned with a p53-sgRNA (Supplementary table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysosome/autophagosome fusion was estimated in ARPE19 cells overexpressing only GFP-LC3 (24920, Addgene) or both GFP-LC3 and AKT2 constructs ...
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bioRxiv - Cell Biology 2023Quote: CRISPR mediated knock-outs were performed by transducing HEK293 cells with LentiCRISPRV2 (Addgene #52961) lentivirus expressing sgRNAs targeting genes of interest (FASTKD5_sg1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and pLenti-PGK-Neo-PIP-FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) (Addgene # 118616) was used for accurate cell cycle phase indicator [34] ...
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bioRxiv - Genetics 2023Quote: ... K562 cells were stably transduced with the pHR-SFFV-dCas9-BFP-KRAB cassette (Addgene plasmid #46911 ...
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bioRxiv - Microbiology 2023Quote: 293TN cells were transfected with the lentiviral envelope and packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with the lentiviral vector pLenti-PGK-ER-KRASG12V (Addgene, 35635) and the packaging plasmids pCMV-VSV-G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... 5 x 106 HEK293T cells were co-transfected with 5μg psPAX2 (Addgene, Plasmid # 12260), 5μg pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... mitochondria were labelled in cells by transduction with pLV-mitoDsRed virus (Addgene, Plasmid 44386) harvested post expression in 293-FT cells and lentiviral particle production ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then transfected with 1 μg of pHrD-IRES-Fluc (Addgene plasmid #194250) and 1 ng of CMV-Rluc reporter plasmids (78 ...
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bioRxiv - Genomics 2023Quote: HaCat dCas9-VPR cells were produced using Lenti-EF1a-dCas9-VPR-puro (Addgene #99373). HaCat dCas9-KRAB-MeCP2 cells were produced using a custom pLV-CBh-dCas9-KRAB-MeCP2- T2A-Puro synthesised by VectorBuilder.
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bioRxiv - Cell Biology 2024Quote: CRISPR-based knockout cell lines were generated using the lentiCRISPRv2 vector obtained from Addgene, which expresses a single guide RNA ...
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bioRxiv - Cell Biology 2023Quote: ... The GIGYF1-KO A549 cells were generated using Lenti-Cas9-Blast (Addgene, plasmid 52962) and pLenti-CRISPRv2 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... PEX3 KO or PEX19 KO cells were co- transfected with pMD2.G (Addgene, 12259), psPAX2 (Addgene,12260 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected 24h before the imaging with Str-Ii_VSVG-SBP-EGFP (Addgene #65300). Live imaging was performed with a spinning disk microscope for 45 min and one frame was acquired every 30 sec ...
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bioRxiv - Synthetic Biology 2024Quote: Phagemid-containing supernatants were added to 2.5 mL S2060 cells (streptomycin-resistant, Addgene #105064) grown to OD600 = 0.5 and allowed to infect at 37 °C and 250 rpm for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were transiently transfected with 1 μg of each plasmid expressing GCaMP6s (Addgene #277314.1040753) (Chen et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... MutuI cells were transduced with lentiparticles derived from pCW-Cas9-Blast (Addgene Plasmid #83481) and selected for stable integration with 10 μg/mL blasticidin ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were co-transfected with the second-generation packaging plasmid psPAX2 (Addgene #12260), envelope plasmid VSV-G (Addgene #12259 ...