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Citations for Addgene :
1151 - 1200 of 1569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... a ChrimsonR-tdTomato fusion cDNA was isolated from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (Addgene #62723) and cloned into pAAV-Ef1a-fDIO EYFP digested as above ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment encoding human 4R1N full length tau were amplified by PCR using tau cDNA (Addgene). And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cyclin D3 cDNA was cloned out of Rc/CMV cyclin D3 (CMV promoter; #10912 from Addgene) with PCR using the primers listed in Table 1 and cloned into pmCherry-C1 (CMV promoter ...
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bioRxiv - Cell Biology 2020Quote: ... VASP cDNA was additionally inserted into the BglII and SalI sites of pmCherry (Addgene ID: 632524). For generation of stably transfected cell lines ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment encoding human 4R1N full length tau were amplified by PCR using tau cDNA (Addgene). And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: The cDNA of the nanobody abGFP4 recognizing GFP (a gift from Brett Collins; Addgene plasmid 49172) [45] was subcloned into a derivative of pET22b vector that lacks the pelB sequence and codes for a C-terminal poly-His tag followed by a cysteine ...
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bioRxiv - Developmental Biology 2022Quote: ... A plasmid containing the full-length human FOS cDNA (NM_005252.4) was purchased from Addgene (Plasmid #59140). The full-length FOS cDNA was cloned into the pCS2 vector and FOS mRNA was generated using the mMessage mMachine Sp6 kit (Ambion ...
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bioRxiv - Cell Biology 2023Quote: ... from human cDNA and cloned into the LeGO-iG2 (kind gift from Boris Fehse, Addgene #27341). YIPF5 ...
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bioRxiv - Cell Biology 2023Quote: ... human ATAD1 was amplified from HEK293T cDNA and cloned into the pKH3 vector (Addgene plasmid #12555). The ATAD1 Walker B mutation (ATAD1E193Q ...
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bioRxiv - Biophysics 2022Quote: The cDNA sequence of the aminoacyl-tRNA synthetase was obtained from the plasmid pANAP (Addgene #48696) (Chatterjee and Guo ...
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bioRxiv - Immunology 2022Quote: ... PCR amplified HGS or VPS37A from U937 cDNA and PCR amplified mScarlet from pmScarlet_C1 (Addgene #85042) into pTRIP-SFFV-EGFP-NLS (Addgene #86677) ...
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bioRxiv - Cancer Biology 2023Quote: ... wild type TP53 cDNA was amplified from p53-GFP plasmid (Cat #11770) (Addgene, Cambridge, Massachusetts, USA) and cloned into pFN21A (Promega Corporation ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNAs were amplified from the corresponding pJet1.2 vectors and pPD95.81 (a gift from Andrew Fire (Addgene plasmid # 1497 ...
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bioRxiv - Cell Biology 2024Quote: cDNA for CENP-T full-length sequence was cloned into pAID1.2-CMV-NmScarlet-mAID (Addgene; #140618). To express the OsTIR1-T2A-BSR and mScarlet-mAID-CENP-T under control of the CMV promoter from the PGK locus ...
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bioRxiv - Cell Biology 2024Quote: H2B-tagBFP was cloned by inserting the H2B cDNA (amplified from pLenti6-H2B-mCherry, Addgene 89766) into the tagBFP-N1 vector (Evrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The EP300 cDNA plasmid in pcDNA3.1-p300 was a gift from Warner Greene (Addgene plasmid # 23252) 84 ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA contained an extra 20-30 bp overlap regions with pAAV-CAG-tdTomato (Addgene #59462). The tdTomato was removed from pAAV-CAG-tdTomato by digestion with KpnI and HindIII ...
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bioRxiv - Cancer Biology 2024Quote: ... SNAI2 cDNA was amplified from U87 cells and directionally inserted into a pCW backbone (Addgene, 50661) rtTA-advanced tetracycline-ON vector system using NheI and BamHI sites ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA for 3xFLAG::CDR2 and 3xFLAG::CDR2L was inserted into pLenti-CMV-GFP-Hygro (Addgene 17446). To generate CDR2/CDR2L single and double KO cells by CRISPR/Cas9 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA for human β1-integrin was obtained by PCR using a template plasmid from Addgene (#69804), the sequence was modified to introduce BamHI and NotI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: ... POLQ-wildtype and POLQ polymerase mutant cDNAs were cloned into pCMMP-MCS-IRES-mRFP (Addgene #36972) by Fast Digest BamHI (#FD0054) ...
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bioRxiv - Cell Biology 2022Quote: ... and mCherry-Snx9 (#27678) (all mouse) were from Addgene.
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bioRxiv - Neuroscience 2020Quote: ... Postsynaptic mouse neuroligin-1 was PCR amplified from Addgene #15260 ...
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bioRxiv - Genetics 2024Quote: Mouse CRISPR Brie lentiviral pooled library (Addgene Plasmid # 170511) consisting of 79,637 gRNAs was co-transfected with packaging plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Biophysics 2023Quote: ... mouse Opa1 sequence was cloned into pR26-CMVconst (Addgene plasmid #127373 ...
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bioRxiv - Neuroscience 2021Quote: ... and SAD-B19 helper proteins (N, 52.11 mg, Addgene 59924; P, 30.15 mg, Addgene 59925; L, 23.70 mg, Addgene 59922; G, 20.26 mg, Addgene 59921). Cells were maintained with DMEM with GlutaMAX supplement ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids coding for pHRIG-Akt1 (Akt-CA) and pHRIG-AktDN (Akt-N) were gifts from Heng Zhao (Addgene plasmids #53583 and 53597 respectively). MAP2-GFP was previously described (Liot et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... with the only exception of ΔR2 and ΔF_ALL_Inv that were generated by using a pair of sgRNA. The guide sequences (listed in Supp. Table 1) were then cloned in px459 CRISPR/Cas9 vector (Addgene Cat. N. 62988), previously digested with Bbs1 ...
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bioRxiv - Systems Biology 2023Quote: ... mCherry-LC3 and mNeon tagged N-terminally with the lipidation sequence of Lck (LckLip-mNeon, the original plasmid was a gift from Dorus Gadella (Addgene plasmid # 98821, (40)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The original bicone construct (A2C-∆ N) was prepared by deleting gvpN from the full GV gene cluster (available on Addgene as plasmid #106473) via KLD mutagenesis using enzymes from New England Biolabs and primers from IDT ...
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bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled clones were expanded before being transfected with Not1-linearized pWZL-hygro H-Ras V12 plasmid (Addgene plasmid # 18749) and selected with hygromycin for 2-4 weeks to generate stable clone ...
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bioRxiv - Cancer Biology 2020Quote: Knockout clones were generated via CRISPR/Cas9 gene targeting by transient transfection with a plasmid encoding hSpCas9 (Addgene #42230) and a plasmid encoding specific gRNA (see below for detail) ...
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bioRxiv - Cell Biology 2022Quote: SIMC1 knockout clones were generated via CRISPR/Cas9 gene targeting by transient transfection of a hSpCas9 encoding plasmid (Addgene) and a pcDNA-H1 plasmid encoding specific sgRNA ...
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bioRxiv - Genetics 2020Quote: ... Gibson assembly was used to clone the Vasa-β-globin-II fragment from the Vasa-Cre plasmid (Addgene; 15885) (Gallardo et al ...
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bioRxiv - Cell Biology 2020Quote: ... designed with the online design tool from the Broad Institute (https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design) were clone into BSbI digested px330 (Addgene #42230), a SpCas9 expressing plasmid (sgRNA ...
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bioRxiv - Neuroscience 2023Quote: ... with CRMP1 then being subcloned into pENTR1A no ccDB (20) (Dr. Eric Campeau, AddGene clone 17398, Watertown, MA, USA). Vectors encoding NPAS3 (13 ...
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bioRxiv - Cell Biology 2023Quote: Previously reported and validated sgRNA targeting exon-10 of G6PD was used to clone into the pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE vector (Addgene #85452). This particular gRNA was transfected using lipofectamine 3000 (Invitrogen #L3000001 ...
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bioRxiv - Genetics 2023Quote: ... a pDestTol2CG2-eye-bfp with the independent marker beta-crystaline:BFP a kdrl P-5’entry clone (Addgene, Santoro Lab), an EGFP-CAAX p-middle entry (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... In-Fusion cloning (TakaraBio) was used to separately clone the different pegRNAs into the piggyBac plasmid vector (Addgene #173222), which contains the prime editor (PE2) ...
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bioRxiv - Cancer Biology 2021Quote: Galectin-3 cDNA was subcloned into the pMIG-GFP retroviral expression vector (plasmid #9044, AddGene, Cambridge, MA). Stably transduced hGalectin-3 expressing OP9 cells were generated by infecting cells with retroviral particles and FACS sorting of GFP expressing (positively transduced ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Neuroscience 2021Quote: ... The OtopLAp cDNA was cloned between the XhoI and XbaI sites of the lexAop vector (Addgene 26224) (29) ...
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bioRxiv - Cancer Biology 2022Quote: pBabe plasmids with EGFR ex19del mutation encoding cDNAs (EGFR E746_A750, EGFR E746_S752>V, EGFR L747_A750>P) and EGFR WT were purchased from Addgene. The empty pBABE-puro retroviral vector or pBABE-EGFR mutants were transfected ...
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bioRxiv - Cell Biology 2022Quote: ... DsRed cDNA under P3 promoter flanked by loxP sites was derived from pHD-DsRed (Addgene plasmid #51434) and placed into the flanking intron that preceded the downstream homology ...
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... full-length hevin cDNA was cloned into the pZac2.1 gfaABC1D-Cyto-GCaMP3 viral vector (Addgene plasmid #44331), between the NheI and NotI restriction sites ...
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bioRxiv - Systems Biology 2020Quote: ... we cloned cDNA for genes of interest into pMXs-gw (Addgene 18656; a gift from Shinya Yamanaka) using BP and LR Clonase II (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: Lentiviral cDNA expression vectors expressing FOXO3.A3 were generated using Gateway cloning in the pINDUCER20 (Addgene #44012) doxycycline inducible expression system (45) ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs for Decorin and E-cadherin (Gene ID: 999) were cloned into modified pHA-N1 (#86049, Addgene) and pcDNA3-neo-Strep-flag-Cterm (#102645 ...
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bioRxiv - Neuroscience 2021Quote: ... we co-transfected neurofilament medium chain (NFM) cDNA-containing plasmid pmNFM (a gift from Anthony Brown, Addgene plasmid #83126 ...