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Citations for Addgene :
1001 - 1050 of 1569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... PV-Cre mice (N = 5) were injected at the same coordinates with 250 nL of AAV-ef1a-Flex-iChloC-2A-dsRed (Addgene plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011 ...
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bioRxiv - Neuroscience 2024Quote: ... Either AAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (n = 7, 200 nL, 3 × 1012 GC/mL, Serotype: 9; Addgene, MA, USA) to express channelrhodopsin (ChR2 ...
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bioRxiv - Neuroscience 2024Quote: ... to express channelrhodopsin (ChR2) or control virus (n = 4, AAV-Ef1a-fDIO-mCherry, 200 mL, 3 × 1012 GC/mL; Serotype: 9; Addgene) was unilaterally injected over 10 minutes into the MePD using a 2-µL Hamilton microsyringe (Esslab ...
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bioRxiv - Neuroscience 2024Quote: ... 7- to 9-week-old male Avpr1b-cre mice and WT mice (n= 24) were injected with 150 nL of the Cre-dependent AAV2-hSyn-DREADD-Gi-mCherry (#44362-AAV2, Addgene) at 5 x 10%+ transducing units per ml at the same coordinates.
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SnoopTag-AffiHER2-SpyTag (N-terminal His6–SnoopTag–anti-HER2 Affibody–SpyTag) (GenBank accession no. KU296975) (Addgene deposition in progress) (Brune et al. ...
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bioRxiv - Neuroscience 2023Quote: Th-cre rats were randomly assigned to a viral group and infused bilaterally with a cre-dependent AAV encoding either ArchT (N = 5; 4 males; AAV5-CAG-FLEX-ArchT-tdTomato, Addgene) or a tdTomato fluorescent protein control (tdTomato ...
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bioRxiv - Neuroscience 2023Quote: Th-cre rats were randomly assigned to a viral group and infused bilaterally with a cre-dependent AAV encoding either ArchT (N = 7; 4 males; AAV5-CAG-FLEX-ArchT-tdTomato, Addgene) or a tdTomato fluorescent protein control (tdTomato ...
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bioRxiv - Neuroscience 2023Quote: Th-cre rats were randomly assigned to a viral group and infused bilaterally with a cre-dependent AAV encoding either the inhibitory opsin archaerhodopsin T (ArchT; N = 11; 6 males; AAV5-CAG-FLEX-ArchT-tdTomato, Addgene) or a tdTomato fluorescent protein control (tdTomato ...
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bioRxiv - Neuroscience 2023Quote: ... University of North Carolina Vector Core) or an enhanced yellow fluorescent protein control (eYFP; N = 13, 6 males; pAAV5-Ef1a-DIO-eYFP, Addgene). Virus (0.2 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Sequences encoding N-KSR or C-KSR were subcloned into SfiI sites of pSBbi-pur-H-2Kb (# 111623, Addgene, USA) replacing an existing H-2kb fragment ...
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bioRxiv - Cell Biology 2022Quote: ... BirA-ACLY constructs were generated by inserting human ACLY into pcDNA3.1 mycBioID vector (N-terminal MYC-BirA, Addgene, plasmid 35700) with a 16 or 46 amino acid linkers between ACLY and BirA ...
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bioRxiv - Developmental Biology 2022Quote: ... or synthesized (NLS and N1ICD the fragment of [ENSMUST00000028288.5] encoding the C-terminal 789 AA of the Notch1 protein) and cloned with sequence encoding an N- or C-terminal eGFP into a modified version of pLKO.3G (Addgene, Cat #14748 ...
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bioRxiv - Molecular Biology 2022Quote: ... a plasmid expressing human Ago2 fused to GFP in the N-terminal region of Ago2 was obtained from Addgene (11590). For recombinant protein purification ...
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bioRxiv - Neuroscience 2023Quote: Ntsr1-Cre mice (n = 3) were stereotaxically injected with an inhibitory opsin (AAV1-hSyn-SIO- stGtACR2-FusionRed, 5 x 1011 vg/ml, Addgene) see “Virus injection and optical fiber implantation” 6 weeks prior to the recordings ...
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bioRxiv - Cell Biology 2024Quote: The monoclonal SK-N-DZ SCLYKO cell line was transduced with Toronto KnockOut (TKO) CRISPR Library - Version 3 (Addgene, 90294) at MOI of 0.3 ...
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bioRxiv - Molecular Biology 2024Quote: The human codon optimized SsdAtox domain was synthetized (Integrated DNA Technologies) and cloned into either the N-terminus or C-terminus of a modified pCMV_BE4max vector (Addgene #112093) with the UGI domain deleted ...
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bioRxiv - Biochemistry 2024Quote: ... were cloned on the N-terminus of monomeric EGFP in the mEGFP-N1 vector (mEGFP-N1 was a gift from Michael Davidson - Addgene plasmid # 54767 ...
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bioRxiv - Cancer Biology 2024Quote: ... GST-SH2 SHC1 (#46486)13 and GST-SH2 (encompassing both the N- and C-terminus) SHP2 (#67575)14 were purchased from Addgene. Phospho-Y222 antibody was developed using peptide CDQRGQSI-pY222-STSFPQP ...
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bioRxiv - Biophysics 2024Quote: ... of STEP (PTPN5) with an N-terminal 6xHis & TEV cleavage site was obtained via Addgene from Nicola Burgess-Brown (Addgene plasmid #39166 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556) ...
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bioRxiv - Cancer Biology 2021Quote: ... The MYC cDNA was cloned into the pCW57.1 vector (gift from David Root (Addgene plasmid # 41393 ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs of the human proteins were cloned into pTT5 based expression vectors (Addgene #52355). The constructs were tagged with Twin-Strep-tag (SII ...
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bioRxiv - Cancer Biology 2020Quote: ... the ZEB1 (NM1174096) cDNA was cloned into the pENTR1A-no ccdb vector (#17398, Addgene) to generate the pENTR1A-ZEB1 vector ...
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bioRxiv - Neuroscience 2020Quote: ... a transfer plasmid containing rat Synapsin promoter and cDNA encoding GCaMP6s (Addgene plasmid #40753) was assembled and transfected with helper-free DJ plasmids (Cell Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA of SKAP2WT and SKAP2W336K (see Table 2) was transferred into pLEX_307 (Addgene #41392). For analysis of subcellular localization ...
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bioRxiv - Neuroscience 2021Quote: ... Lentivirus encoding GFP or TTL cDNA (packaging vectors, pWPT-based vector, Addgene, Cambridge, MA) and shTTL1 ...
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bioRxiv - Biophysics 2022Quote: The gene with codon-optimized human dysferlin cDNA was obtained from Addgene (Plasmid 67878) [47] ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA from RPE1 cells was cloned into pLenti CMVie-IRES-BlastR (Addgene plasmid #119863). PAXIP1 W75R ...
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bioRxiv - Physiology 2023Quote: ... HyPer7.24 The cDNA of pCS2+MLS-HyPer7 was purchased from Addgene (Plasmid ID: 136470) and was transfected in HAECs using the NeonTM Transfection System (Invitrogen ...
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bioRxiv - Pathology 2023Quote: cDNAs encoding WT and W70X-prestin were cloned into a pSBtet-pur vector (Addgene) using SfiI sites with or without a C-terminal mTurquoise (mTq2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The mutant cDNAs were transferred by Gateway reaction into pLENTI PGK Neo DEST (Addgene #19067 ...
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bioRxiv - Molecular Biology 2023Quote: ... All cDNA was cloned in frame into the pCS2+8CmCherry vector (Addgene Plasmid #34935) using AscI (NEB #R0558 ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing human BRCA1 cDNA were a gift from Junjie Chen (Addgene, Plasmid #99394). We cloned the BRCT and RING variant library by designing primers (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... PCR amplified ScTop2 and HsTOP2α cDNAs were inserted into the 12URA-B (Addgene #48304) yeast expression vector while HsTOP2β was inserted into the 12URA-C (Addgene #48305 ...
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bioRxiv - Cell Biology 2023Quote: ... elegans cDNA library and cloned into UC Berkeley Macrolab vector 2-CT (Addgene #29706), which encodes a TEV protease-cleavable His6-MBP (maltose binding protein ...
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bioRxiv - Genetics 2023Quote: ... HCASMC were transduced with 2nd generation lentivirus with cDNAs cloned into pWPI (Addgene, 12254) using NEBuilder HiFi cloning (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral JAK2 expression vector was obtained by cloning JAK2 cDNA (Addgene plasmid 23915) into a modified pLX303 vector that co-expressed myc-tagged mCherry through a P2A site ...
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bioRxiv - Cell Biology 2024Quote: ... we inserted human LC3/GBRP cDNA in a pGEX-4T1 vector (RRID:Addgene_223726; RRID:Addgene_216836; RRID:Addgene_223727 ...
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bioRxiv - Cancer Biology 2024Quote: ... STAT1 Y701F or STAT1 S727A cDNA were obtained from Addgene (plasmid #12301,12302 and 12304)52 ...
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bioRxiv - Cancer Biology 2024Quote: ... wild-type DAXX cDNA was cloned into the pInducer20 expression vector (Addgene Plasmid #44012), lentivirus was generated using the psPAX and pMD2.G packaging vectors (Addgene Plasmid #12260 and #12259 ...
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bioRxiv - Cancer Biology 2022Quote: All SUM149PT clones and parental line were infected with high titer pHIV-Luc-ZsGreen (Addgene #39196) virus so as to generate ZsGreen and Luciferase expressing tumor cells for metastasis tracking in mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... and shINTS7 single clones were established by lentiviral infection with Tet-pLKO-puro vector (Addgene, #21915) containing the respective shRNA sequences (shINTS3 ...
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bioRxiv - Neuroscience 2021Quote: ... Entry clones were then recombined with pDEST-527 (a gift from Dominic Esposito (Addgene plasmid # 11518)) in LR reactions generating expression clones ...
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bioRxiv - Molecular Biology 2023Quote: ... Three clones with the correct insertion were transiently transfected with either 5 μg pFlpO (Addgene #13793) to remove the neomycin selection cassette or both 5 μg pFlpO (Addgene #13792 ...
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bioRxiv - Molecular Biology 2024Quote: ... Bacterial clones expressing double-stranded RNA (dsRNA) included the control (empty vector pL4440) sourced from Addgene, Cambridge ...
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bioRxiv - Synthetic Biology 2023Quote: ... This plasmid was used as a template to clone pCOLA-Gent-yibD-Erv1p (Addgene Cat#202483) with gibson assembly ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... All clones for lentiviral vectors expressing the proteins described in this study are available from Addgene.
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bioRxiv - Biochemistry 2024Quote: ... entry clones were cloned into pAG415GPD-ccdB (Addgene plasmid #14146; http://n2t.net/addgene:14146; RRID:Addgene_14146, [50]) using Gateway cloning (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... target cells were derived by transfection with plasmids designed to express the SARS-CoV-2 D614 Spike protein with a c-terminus flag tag (kindly provided by Dr. Farzan, Addgene plasmid no. 156420 (Zhang et al., 2020)) ...