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Citations for Addgene :
1101 - 1150 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus production was obtained by co-transfecting 293FT cell line with psPAX2 (Addgene, plasmid #12260), pCMV-VSVG (Addgene ...
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bioRxiv - Neuroscience 2020Quote: HeLa cells were plated on glass coverslips and transfected with PSD95-tRFP (Plasmid #52671, Addgene) and/or EGFP-tagged SynGAP C-terminal constructs (EGFP-CCα1 or EGFP-CCPBM plasmids (made in house) ...
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bioRxiv - Microbiology 2021Quote: ... by flow cytometry on HEK293-FT cells transiently expressing SARS-CoV-2 N (Addgene # 141391), S (BEI # NR-52310 ...
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bioRxiv - Immunology 2021Quote: ... were transfected in HEK293T cells with packaging vectors pMD2G and psPAX (Addgene 12260 and 12259) to generate viral particles ...
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bioRxiv - Genetics 2020Quote: ... The plasmids were packaged into lentivirus by co-transfecting HEK293T cells with pPAX2 (Addgene, #35002) and pMD2.G (Addgene ...
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bioRxiv - Immunology 2022Quote: MSP2N2-His6 protein (45) was expressed by transforming BL21 DE2 cells with pET28-MSP2N2 (Addgene). Cells were grown to a OD600 of ~0.8 in the presence of 50 μg/mL of kanamycin and induced for 3 hours with 0.5 mM isopropy β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Molecular Biology 2022Quote: ... CALU6 cells were infected with a genome-wide CRISPR library (Addgene, (Wang et al., 2015)) or CALU6 and H1975 cells were infected with a metabolism focused CRISPR library (Addgene,(Birsoy et al. ...
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bioRxiv - Cancer Biology 2022Quote: MB-004 cells were transduced with the enti-dCas9-KRAB-T2A-GFP virus (Addgene # 71237). sgRNAs targeting SOX11/HNRNPH1-binding motifs in the distal enhancers were designed using the CHOPCHOP program (https://chopchop.cbu.uib.no) ...
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bioRxiv - Cancer Biology 2022Quote: ... CHAC1 and SLC25A39 knockout cells were generated using the pLenti-CRISPR-V2 plasmid (Addgene, 52961) encoding validated sgRNA sequences towards CHAC1 or SLC25A3974 ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were plated 1 day before transfection with psPax2 (viral packaging plasmid; Addgene; 12260), pCMV-VSV-G (viral envelope plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... then co-transfected into HEK293T cells with lentiviral packaging plasmids psPAX2 and pMD2.G (Addgene, 12260 and 12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... Knockout HIC1 HC cell lines (HIC1 KO) were generated using the lentiCRISPRv2 vector (Addgene, #52961); HIC1-specific single guide RNA (sgRNA ...
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bioRxiv - Microbiology 2022Quote: ... Pseudovirions were generated by co-transfection of HEK293T cells with MLV-gag/pol (Addgene #14887) and MLV-Luciferase (Addgene #170575 ...
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bioRxiv - Neuroscience 2022Quote: ... Transiently transfected cells were induced to express by co-transfecting 300ng of pAC-GAL4 (Addgene) and analyzed at 48hr post-transfection.
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bioRxiv - Pathology 2024Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
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bioRxiv - Cancer Biology 2024Quote: ... tIMEC-A-H1 cell line was generated by stable nucleofection of ppyCAG_RNaseH1_WT plasmid (Addgene, 111906)16 using the P1 Primary Cell 4D-Nucleofector X kit (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: The GBP1-GFP HeLa cell line was constructed using the LentiCRISPR V2-Blast (Addgene #83480) vector containing Cas9 sequence and a gRNA targeting exon 11 of the GBP1 gene that encodes the stop codon (See Table S1 for gRNA sequence) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells were transfected with the DNA of lentiviral packaging plasmids vSVG (Addgene, USA, #8454), RSV (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were transfected with the DNA of lentiviral packaging plasmids vSVG (Addgene, USA, #8454), RSV (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with the pCAG-eSpCas9-2A-GFP (generous gift from Jizhong Zou, Addgene plasmid #79145 ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8 ...
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bioRxiv - Cancer Biology 2024Quote: Stable cell lines were generated by retroviral infection with pBABE-puro (1764, Addgene, Cambridge, MA), pBABE-zeo (1766 ...
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bioRxiv - Cell Biology 2024Quote: ... B16-F1 cells were transiently transfected with LifeAct-TagRed and PA-GFP-Actin (Addgene #57121) as described above ...
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bioRxiv - Cell Biology 2024Quote: ... AICSDP-42:AAVS1-mTagRFPT-CAAX was a gift from Allen Institute for Cell Science (Addgene plasmid # 107580 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were co-transfected with 2 μg lentivirus packaging plasmids (pMD2.G (Addgene #12259) and 10 μg psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2024Quote: 1099 or pB3 cell line derivatives expressing pCDH-EF1-Luc2-P2A-tdTomato (Plasmid #72486, Addgene) were resuspended at a concentration of 1×106/mL in culture medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Therefore, we used lipofection (Lipofectamine 3000, #L3000001) of HEK293T cells to package psPAX2 (Addgene, #12260), pMD2.G (Addgene ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells expressing transiently-transfected actin fused to monomeric azure-fluorescent protein (mAzure-Actin; Addgene) were grown on coverslips in 12-well plates at 2×105 cells/well and infected with Bp340 ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were co-transfected with 3 plasmids: pAd-DELTA F6 (plasmid No. 112867; Addgene), serotype plasmid AAV PHP.eB ...
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bioRxiv - Cell Biology 2023Quote: ... Pitx2-GFP expressing cells were cloned into pLenti PGK Neo DEST (Addgene plasmid # 19067 ref68), EGFP was cloned at the C terminus of the coding sequence of Pitx2 isoform 1 (Origene ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were transduced with a luciferase lentiviral vector (pHIV-Luciferase, Addgene #21375). Following puromycin drug selection ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 x 105 cells from the previous transfection were transfected with pCMV-PEmax (Addgene: 174820)11 (800 ng ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were generated using the lentivirus Trono group second generation packaging system (Addgene) and selected using puromycin resistance (2 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were co-transfected with transfer plasmids (pCMV-VSV-G and delta8.9, Addgene #8454) and standard packaging vectors using the TransIT-LT1 Transfection Reagent (Mirus ...
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bioRxiv - Cancer Biology 2023Quote: SUDHL-4 cell line was infected using pLKO.1-puro-GFP U6 sgRNA (Addgene #50920) containing BAX RNA guides or non-targeting RNA guide:
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bioRxiv - Cancer Biology 2023Quote: ... or Lifr were generated by transducing cells with the plasmid lenticrispr V2 puro (Addgene #98290) cloned with a specific guide ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with plasmids encoding hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP (Addgene plasmid #40640) or pcDNA3.1(- ...
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bioRxiv - Immunology 2023Quote: ... Platinum-E retroviral packaging cells were transfected transiently with modified pMIG retroviral plasmids (Addgene, #9044) or a second-generation anti-hCD19 CAR construct (MSCV-myc-CAR2A-Thy1.1 ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviruses were produced by transfecting Lenti-X 293T cells with pMD2.VSVG (Addgene plasmid # 12259) and psPAX2 (Addgene plasmid # 12260).
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bioRxiv - Microbiology 2023Quote: ... cells were co-transfected with 900 ng of the I-SceI plasmid (pCBASceI, Addgene #26477) and 900 ng of pcDNA4/TO-HHV-6B IE1 (+I-SceI ...
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bioRxiv - Biophysics 2023Quote: TIA1 proteins were expressed in the BL21DE3 bacterial cell line from the pET28b plasmid (Addgene) containing the Mus musculus (mouse ...
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bioRxiv - Cancer Biology 2023Quote: AW13516 cell lines stably expressing either non-targeting scrambled shRNA (#1864) (Addgene, Cambridge, Massachusetts, USA) or shRNAs targeting C1QBP or TRIM23 were employed to study the effect of knockdown on the tumorigenic potential of cancer cells ...
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bioRxiv - Cancer Biology 2023Quote: ... These constructs were packed into lentivirus by transient transfection in 293T cells with psPAX2 (Addgene) and the ecotropic MLV envelope pCAG-Eco using calcium phosphate as described (57) ...
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bioRxiv - Systems Biology 2022Quote: ... 240 million cells were transduced with lentivirus from the Human Genome-wide CRISPRi-v2 (Addgene #83969 ...
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bioRxiv - Systems Biology 2023Quote: ... All pEN_TT donor vectors were LR recombined with either pSLIK zeo (B2B1 cells; Addgene #25736) or pSLIK neo (TM15c6 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... ANBL6 cells were infected with lentiviruses carrying pCMV-T7-SpCas9-P2A-EGFP (Addgene, plasmid # 139987) construct and GFP+ cells were selected using FACS.
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bioRxiv - Cancer Biology 2023Quote: ... CHMp and CHMm cells were transfected with a firefly luciferase gene-carrying plasmid (Addgene #18964) using Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells were co-transfected with Cas9-mCherry (pU6-CBh-Cas9-T2A-mCherry: Addgene 64324) and gRNA-BFP (pKLV2.2-h7SKgRNA-hU6gRNA-PGKpuroBFP ...
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bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells were co-transfected with Cas9-BFP (pU6-CBh-Cas9-T2A-BFP: Addgene 64323) and gRNA (pSPgRNA ...
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bioRxiv - Neuroscience 2023Quote: ... lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 49 (Addgene #96918 ...