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Citations for Addgene :
851 - 867 of 867 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... was cloned by generating PCR fragments from the pTetQCas-8+IS186 and the pTnsABC construct (gift from Sheng Yang (Addgene plasmid # 170636; & #1306331)) using the GeneArt™ Gibson Assembly HiFi Master Mix (InvitrogenTM) ...
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bioRxiv - Microbiology 2021Quote: ... the open reading frame was amplified by PCR from the pDONR223 SARS-CoV-2 NSP5 plasmid (Addgene, #141259, a gift from Fritz Roth [32]), including an ATG start codon in the forward primer and a TAA stop codon in the reverse primer (MPro_Fw ...
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bioRxiv - Biophysics 2020Quote: ... The pCNDA3.1-jRGECO1a plasmid was cloned by assembling PCR-amplified fragments from pGP-CMV-NES-jRGECO1a (Addgene # 61563, a gift from Dr. Douglas Kim57) and pCDNA3.1 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid pVpr.IN.mScarlet encoding a Vpr.IN.mScarlet fusion protein with a HIV-1 protease recognition site between Vpr and IN was constructed from pVpr.IN.eGFP (Albanese et al., 2008) by PCR amplification of the mScarlet gene from pmScarlet C1 (Addgene Cat#85042; (Bindels et al., 2017)) (primers used for PCR ...
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bioRxiv - Genetics 2022Quote: Human PUM1 full-length cDNA was amplified by PCR and subcloned in a pRK5 plasmid containing the Myc tag sequence (Addgene, pRK5-Myc-Parkin #17612) at the N-terminal by using SalI (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The coding sequence for the α-ALFA NB was amplified by PCR from the mEGFP-NbALFA plasmid (Addgene plasmid #159986; (Jin, et al. 2021)) using the primers P3 and P4 ...
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bioRxiv - Microbiology 2023Quote: ... The coding sequence for the Tn903.1 was amplified by PCR from the pET28 (T7pCONS/TIR2)-sfGFP plasmid (Addgene plasmid #154464;(Shilling, et al. 2020)) using the primers P7 and P8 ...
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bioRxiv - Molecular Biology 2024Quote: ... a 24-well dish was transfected with 250ng of px33050 containing the RBBP6-targeting guide and 250ng of PCR product containing the dTAG degron preceded by a blasticidin or puromycin selection marker (derived from Addgene plasmid 91792 and 9179324). Jetprime (Polyplus ...
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bioRxiv - Genetics 2024Quote: ... The denAsCas12a open reading frame was PCR amplified from pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) (Addgene plasmid # 107943, from 31). AsCas12a variants described were generated by using the denAsCas12a open reading frame as starting template and introducing the specific mutations encoded in overhangs on PCR primers that serve as junctions of Gibson assembly reactions ...
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bioRxiv - Bioengineering 2022Quote: ... the modules in these plasmids (split Cas9, MCP, sgRNA cassette) were PCR-amplified and recloned into 2 lentiviral plasmids (pLKO.1 neo, Addgene #13425; pLJM-EGFP, Addgene #19319) and ...
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bioRxiv - Microbiology 2020Quote: ... pTRIP-SFFV-tagBFP-2A-hACE2 and pTRIP-SFFV-TagRFP657-2A-TMPRSS2 constructs were obtained by PCR from pLenti6-hACE2-BSD (hACE2 sequence from Addgene #1786 subcloned into pLenti6-BSD) and pCSDest-TMPRSS2 (Addgene #53887 ...
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bioRxiv - Microbiology 2020Quote: ... A miniTn7 element containing dcas9 was constructed by PCR-amplifying the tetR-tetP-dcas9-rrnBT1-T7Te fragment from pdCas9-bacteria (Addgene #44249, gift of Stanley Qi) with primers containing SpeI and PstI sites ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplifications of three PCR fragments containing four gRNA sequences (Fig. S5) with the respective primers and pCFD6 as a template (Addgene plasmid # 73915; from Simon Bullock), followed by Gibson assembly with the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: The sequences encoding STIM1 and RspA-NFAST were amplified by PCR from respectively the human STIM1-YFP plasmid (Addgene #19754, a gift from A. Rao) and the pAG573 FRB-RspA(N)-IRES-mTurquoise214 plasmid ...
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bioRxiv - Cell Biology 2024Quote: To generate RRBP1 KO HEK293T cells expressing EGFP-FLAG-APEX2-ePTS1 single gRNA transcriptional cassette prepared by PCR and pCAS9-mCherry empty (Addgene, 80975 (Schmid- Burgk et al., 2016)) were co-transfected into HEK293T cells expressing EGFP-FLAG-APEX2- ePTS1 ...
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bioRxiv - Microbiology 2024Quote: ... Roughly 1,000 base pairs upstream and downstream of the desired deletion location were amplified via PCR and assembled into allelic exchange vector pIMAY (Addgene plasmid # 68939, gifted by Tim Foster) (69 ...
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bioRxiv - Microbiology 2021Quote: ... The coding sequence from amino acid 747E to 1061K was amplified by PCR from the pDONR207 SARS-CoV-2 NSP3 plasmid (Addgene, #141257, a gift from Fritz Roth[32]), including a Kozak sequence and ATG start codon in the forward primer and a TAA stop codon in the reverse primer (PLPcd_Fw ...