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Citations for Addgene :
651 - 700 of 867 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... were cloned by PCR amplification of human LGals3 and LGals8 cDNAs from the pHAGE-mKeima-LGALS3 and pHAGE-FLAG-APEX2-LGALS8 plasmids (Addgene plasmids #175780 and #175758 ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting 4xU6:sgRNA sequence was PCR amplified and ligated into the backbone of pDestTol2pA2-U6:gRNA (Addgene plasmid 63157) after the vector was first digested with ClaI and KpnI to generate the pDestTol2pA2-4xU6:sgRNA plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... followed by digestion of amplified PCR product with AgeI and NotI and ligation C-terminal to Smo in Smo-mCherry (Addgene plasmid 55134 ...
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bioRxiv - Cell Biology 2024Quote: An expression vector for Akt-PH-Clover was constructed as follows: The DNA encoding Clover was PCR-amplified using pcDNA3 Clover (Lam et al., 2012)(Addgene #40259 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.Dlx.DIO.GFP.N2cG was constructed by Gibson assembly the PCR fragment GFP-P2A-N2cG from pAAV-VTKS2-TVA-eGFP-N2cG (Addgene #175439) into the backbone pAAV-VTKD2 (Addgene #170847)65 digested by EcoRI and HindIII.
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bioRxiv - Microbiology 2024Quote: ... The cDNA of interest was amplified by PCR using primers (Table S4) with overhangs that allow directional cloning into EcoRV-linearized pHIVdTomato (#21374, Addgene) using the 2X Gibson Assembly Kit (Cat # E2611S ...
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bioRxiv - Cancer Biology 2024Quote: ... A sequence containing the 2.4-kbp p21 promoter region was PCR amplified from the pGL2-p21 promoter- Luc (Addgene #33021). The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a- mCherry-3X-NLS (Addgene #125262) ...
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bioRxiv - Cell Biology 2020Quote: ... were annealed in annealing buffer (10mM Tris pH 8.0, 50mM NaCl, 1mM EDTA) and cloned in pENTR/pSUPER+ entry vector (Addgene#17338) which was recombined into pLenti X1 Puro DEST (Addgene#17297) ...
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bioRxiv - Plant Biology 2019Quote: ... K353E, and D450N), RBOHD/C (full-length, C1, C2, and C3) were amplified by PCR and cloned into pOPINK (Addgene, #41143) or pOPINM (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... A C-terminal Strep tag on ORF24-NTD was added by inverse PCR to generate p6H-SUMO3-ORF24-NTD-Strep (Addgene #138467).
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bioRxiv - Cell Biology 2020Quote: For derivation of HA-Smo and Smo-HA ESCs mouse Smo was amplified by PCR from the pGEN-mSmo (Addgene, #37673) and introduced in the PB-HA-IRES-Neo vector ...
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bioRxiv - Cell Biology 2020Quote: ... To generate PCP-NLS-2xmCherry, we PCR amplified PCP from phage UbiC NLS HA stdPCP st-dGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-ATACTCGAGCGCCAC-CATGGGCCCAAA and 5’-CTCATCGATGGTGGC-GACCGGTGGAC ...
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bioRxiv - Cell Biology 2020Quote: ... To generate MCP-NLS-2xGFP, we PCR amplified 2xEGFP from phage UbiC NLS HA stdPCP stdGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-TCTATCGATATGGTGAGCAAGGG-CGAG and 5’-ATAGCGGCCGCTTATTTGTACAAT-TCATCCATACCATGGG ...
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bioRxiv - Cell Biology 2020Quote: ... To generate the modified TREAT reporter constructs, we first PCR amplified humanized Renilla luciferase from the original TREAT plasmid (Horvathova et al., 2017) (Addgene #104096) using primer sequences 5’-ATACTCGAGCGACTCACTATAGGCTAGCCAC and 5’-ATAGGCCGGCCTTACTGCTCGTTCTTCAG-CAC ...
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bioRxiv - Cell Biology 2020Quote: ... We then excised the IRES-H2B-RFP cassette and cloned in place a 5’ copy of YFP lacking a stop codon, which we PCR amplified from IRES-H2B-YFP-DD (Han et al., 2014) (Addgene #96893) using primer sequenc-es 5’-AAGTTATCGATATGGTGAGCAAGGGCGAGG and 5’-GCATGGACGAGCTGTACAAGAGCGGC-CGCATGGT ...
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bioRxiv - Genetics 2021Quote: ... For the pPB_TRE3G::dCas9-5XGCN4_EF1a::TetOn-Hygro, the Streptococcus pyogenes dCas9GCN4 was PCR amplified from the PlatTET-gRNA2 plasmid (Morita et al., 2016) (Addgene #82559), and cloned together with a d2 destabilization domain under control of the TRE3G promoter in a PiggyBac backbone vector also containing the TET-ON3G transactivator and the Hygromycin resistance gene separated by an IRES sequence and under control of the CAG promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... To express TagRFP658 and mCardinal in neurons under CaMKII promoter the corresponding genes were PCR amplified and swapped with the Arch-GFP gene in FCK-Arch-GFP (Addgene #22217) using InFusion cloning ...
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified from the pBid-UAS-myr plasmid (Akbergenova et al., 2018) and fused with the GCaMP7s cDNA (Addgene # 104463) (Dana et al. ...
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bioRxiv - Neuroscience 2021Quote: ... The donor was made by amplifying homology arms from the genome by PCR and fusing them by Gibson assembly with a cDNA coding for 6xHis-mClover3 (Addgene #74252) (Bajar et al. ...
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bioRxiv - Molecular Biology 2021Quote: We in-house generated a lentiviral construct expressing dCas9-KRAB-MeCP2 by PCR amplification of the dCas9-KRAB-MeCP2 (contains a domain of MeCP2) from pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene 110824), and then insert it to the pLenti-EF1a-dCas9-VP64-2A-Blast backbone (Addgene 61425 ...
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bioRxiv - Cell Biology 2022Quote: CymR was constructed via PCR and Gibson Assembly from the following constructs: pCuo CA Rac1 CMV + cumate operon purchased from Addgene (#84643), human APC open reading frame purchased from Addgene (#16507) ...
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bioRxiv - Cell Biology 2022Quote: ... The mCherry2-NSP2 expressing plasmids were created by cloning a PCR-amplified NSP2 fragment into the mCherry2-C1 (Addgene plasmid 54563) and mCherry2-N1 vectors (Addgene plasmid 54517 ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP ORF was PCR-amplified and cloned between AgeI and EcoRI sited of lentiviral vector with synapsin I promoter (Addgene #20945). During this cloning AgeI site was destroyed and a new AgeI was introduced on a PCR primer downstream of GFP ...
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bioRxiv - Genomics 2020Quote: ... The INTS6 ORF was PCR amplified with 5’ Xho I and 3’ EcoRI restriction site overhangs and the coding sequence for a C-terminal V5 epitope tag was added in frame to the 3’ end of the INTS6 ORF (Key Resources Table. The INTS6 PCR product and MSCVpuro vector (Addgene 68469) were digested with XhoI (NEB R0146 ...
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bioRxiv - Molecular Biology 2019Quote: ... The linear donor was generated by PCR and gel purified from a plasmid harboring a synthetic AID-P2A-Hygromycin insert (pMGS54, Addgene #126583). We amplified the insertion using primers that contain 50 nucleotide homology tails ...
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bioRxiv - Genomics 2019Quote: ... the NLSSV40-dCas9-3X NLSSV40-24X GCN4-V4-NLSSV40-P2A-BFP fragment was amplified by PCR from plasmid pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (Addgene Plasmid #60910) and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-PuroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB) ...
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bioRxiv - Systems Biology 2019Quote: ... we mixed competent yeast with promoter and GFP or mCherry PCR products and 60ng of linearized expression vector BSP188 (Addgene #110917). This expression vector contains the unc-54 terminator and chromosome II MosSCI homology arms for integration at ttTi5605 ...
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bioRxiv - Molecular Biology 2019Quote: The pLenti-EF1-osTir1-9Myc-P2A-Bsd was constructed by PCR amplifying the Oryza Sativa Tir1 (osTir1) cDNA from the pBabe Puro osTIR1-9Myc (Addgene #80074) and inserted into the AgeI/BamHI site of the lentiCas9-Blast construct (Addgene #52962).
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bioRxiv - Cell Biology 2019Quote: ... BbsI digested fragment containing gRNA core and dU6:3 promoter was PCR amplified from pCFD4-U6:1_U6:3-tandemgRNAs (gift from Simon Bullock (Addgene plasmid # 49411) (Port et al. ...
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bioRxiv - Bioengineering 2021Quote: ... The nCas9(D10A) was generated by the PCR method using previously optimized Cas9 as a template (Level 1 hCas9 module, Addgene #49771). Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966 ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The UAS sites and K10 3’UTR were replaced via PCR with the squash promoter and squash 3’UTR from pBS-Squ-mCherry (Eric Wieschaus56, Addgene 20163). The RhoGEF2 ORF was obtained from the DGRC (SD04476) ...
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into Ac5-Stable2-neo (Rose Barrio and James Sutherland60, Addgene 32426).
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into pGEX6P1-N-HA (Andrew Jackson and Martin Reijns, Addgene 119756).
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bioRxiv - Neuroscience 2020Quote: ... pAAV-hsyn1-mGFP was generated by inserting PCR-amplified mGFP between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881). The plasmid pAAV-Ef1a-DIO-hChR2(E123T/T159C)-EYFP was obtained from Addgene (#35509).
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bioRxiv - Neuroscience 2020Quote: To generate pAAV-hsyn1-mGFP-T2A-Cre we amplified EGFP-T2A-Cre by PCR from pRetroX-GFP-T2A-Cre (Addgene #63704) and inserted this fragment between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881) ...
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bioRxiv - Biochemistry 2020Quote: ... BsaI sites were added to ends by PCR with complementary overhangs for golden gate cloning into pATT-Dest (Addgene plasmid #79770) using primers MBP-BsaI-GG-F and MBP-BsaI-GG-R (the sequences for all primers used for cloning can be found in Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... The expression vector for N-terminally FLAG-tagged mouse SYDE2 was generated by PCR amplification of the coding sequence from pNICE HA-mSYD1B (Addgene #59362) and insertion into pcDNA3-FLAG by Gibson assembly.
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bioRxiv - Cell Biology 2021Quote: ... Mito-mCh-1×FLAG and Mito-mCh-smFLAG were constructed by ligating 1×FLAG synthesized by overlapping PCR and smFLAG amplified from smFLAG-KDM5B-24×MS2 (Addgene # 81084) with previously built Mito-mCh-1×HA cut by BglII and BamHI through Gibson Assembly.
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bioRxiv - Neuroscience 2020Quote: ... Rnd2 and DNRhoA were cloned by PCR using pNeuroD1-Rnd2 (Heng et al., 2008) and pRK5-myc-RhoA-T19N (Addgene 12963) as templates respectively and then inserted into the SfiI/PmeI sites of the CAG-Neurog2-IRES-DsRed retroviral vector (Heinrich et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNA resistant ORC2 (ORC2gr) was amplified by PCR and cloned into NheI-digested mAID-mCherry2-NeoR plasmid (mAID-mCherry2-NeoR, Addgene 72830) in order to add mAID degron sequence to the N-terminus ...
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bioRxiv - Plant Biology 2021Quote: ... 3356 bp upstream of the RPT2 start codon were amplified by PCR with primers 13Rb-RPT2F and 13Rb-RPT2R and cloned into the AL13Rb plasmid (Addgene # 161006) (Alamos et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA encoding RNF43 without stop codon was amplified by the PCR and cloned into the pcDNA3.1 MCS-BirA(R118G)-HA (Addgene plasmid #36047) using HpaI (ER1031 ...
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bioRxiv - Microbiology 2022Quote: ... with a 15 base pair overlap with vector backbone were used in a polymerase chain reaction (PCR) to generate fast Timer protein timer fragment from plasmid pFast-FT-N1 (Addgene 31910). Five tandem repeats of Tax responsive element (TRE ...
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bioRxiv - Neuroscience 2022Quote: ... Human TAOK2α was PCR amplified from pCMV-Sp6-TAOK2 plasmid (Ultanir et al., 2014) and cloned into the sfGFP-C1 vector (Addgene #54579) using restriction sites HindIII and MfeI ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting PCR amplicon was assembled with a level 0 part encoding either an Arabidopsis U6-26 promoter (AtU6-26 Addgene#68261) or a N ...
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bioRxiv - Biophysics 2022Quote: Human kinesin-5 (Kif11/Eg5) 5-513 was PCR amplified from mCherry-Kinesin11-N-18 plasmid (gift from Michael Davidson, Addgene # 55067). This fragment was previously shown to form functional dimers [19] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We next PCR amplified the LexA promoter (LexApr) using oligos 1195 and 1240 from FRP1642 (Addgene #58442, Ottoz et al. 2014). We then cloned both the promoter and the wtf6 poison CDS fragment into pRS316 (Sikorski and Hieter ...
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bioRxiv - Developmental Biology 2020Quote: ... MCP-TagRFPT constructs were assembled by replacing the eGFP fragment of pNosPE_MCP-eGFP using NheI/BamHI with the TagRFPT coding sequence amplified by PCR (supplementary table 1) from TagRFP-T-Rabenosyn-5 (Addgene 37537). MCP-TagRFPT-NLS was generated by insertion of the TagRFPT-NLS coding sequence into pNosPE_MCP-eGFP with NEBuilder® HiFi DNA Assembly Master Mix (primers listed in supplementary table 1).
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified and cloned downstream of EGFP in the pAc5.1B-EGFP vector (a gift from Elisa Izaurralde; Addgene plasmid #21181) using the HindIII and EcoRI restriction sites to make pAcB5.1-EGFP-FMRP ...