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Citations for Addgene :
751 - 800 of 1569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and the triple HA tag (gray highlighted text) replaced the mEGFP-cmak2a HDR cassette in the backbone of the pAAV-HDR-mEGFP-camk2a (Addgene #104589). This construct ...
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bioRxiv - Neuroscience 2020Quote: ... with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Previously described pMIG-Aalpha WT retroviral vector with a Flag-tag at the NH2 terminal (32) obtained from Addgene (plasmid #10884) was generously provided by William Hahn ...
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bioRxiv - Biochemistry 2022Quote: ... plasmid to produce a construct carrying a N-terminal hexahistidine and hexahistidine-SUMO tag followed by a TEV cleavage site and the ShTniQ was cloned into the 1C (Addgene 29659) vector generating a construct carrying a hexahistidine-maltose binding protein (6xHis-MBP ...
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bioRxiv - Cell Biology 2022Quote: ... The mammalian expression plasmid for Ism1 with C-terminal myc-6xhis tag plasmid for recombinant Ism1 protein production was from Addgene (#173046).
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bioRxiv - Molecular Biology 2020Quote: ... Full-length BcRF1 was PCR amplified from pcDNA4/TO-BcRF1-3xFLAG (19) and cloned into the BamHI and XhoI sites of pcDNA4/TO-2xStrep (C-terminal tag) using InFusion cloning (Addgene #138436). Mutations of the RLLLG motif in UL87 ...
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bioRxiv - Molecular Biology 2020Quote: ... Full-length mu24 was PCR amplified from MHV68-infected 3T3 cell cDNA with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag) using T4 DNA ligase (Addgene #138435). Full-length BcRF1 was PCR amplified from pcDNA4/TO-BcRF1-3xFLAG (19 ...
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bioRxiv - Genetics 2020Quote: Human codon-optimized high fidelity Cas9 nuclease construct with GFP tag (pSpCas9(BB)-2A-GFP (PX458))80 was obtained from Addgene (#48138). Two sgRNAs were designed using CRISPR-Cas9 guide RNA design checker from Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... A gBlock containing sequence for SNAP-V5 tags was inserted into pLenti CMVTRE3G eGFP Blast (w818-1) (gift of Eric Campenau, Addgene #27568) at the AgeI restriction site using Gibson Assembly to make pLTRE3G-SNAP-V5-eGFP ...
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bioRxiv - Cell Biology 2020Quote: hCIB2 was subcloned into pET21a (histidine tag on the C-terminus; hCIB2-6xHis) and GST-Rheb in pGEX-4T-2 (Addgene #15889), transformed in Bl2 (DE3 ...
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bioRxiv - Genomics 2024Quote: ... and the top two scoring sgRNAs for each target gene based on depletion or enrichment phenotypes were cloned into dual sgRNA lentivirus expression vectors with direct capture tags (Addgene 187241)40 using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621L ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA fragment of tight TRE promoter with ATG start codon and 3XFLAG tag was amplified from pCW-Cas9 (Addgene #50661). The DNA fragment of hPGK-PuroR-rTetR was amplified from pCW-Cas9 ...
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bioRxiv - Biochemistry 2023Quote: ... a recombinant fragment comprising part of the CM1 motif of human CDK5RAP251-100 (also referred to as γ-TuNA [32]) fused to a cleavable GFP tag (His6-SUMO-TEV-GFP-PreScission-γ-TuNA, a gift from Tarun Kapoor (Addgene plasmid # 178066 ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Rab7A and SNAP-Rab7A Q67L were made by cloning Rab7A sequence from EGFP-Rab7A and EGFP-Rab7A Q67L plasmids into pSNAP-tag (m) Vector (Addgene #101135). Soluble BFP (mTagBFP2 ...
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bioRxiv - Biochemistry 2023Quote: ... The codon optimized AOX with a C-terminal HA tag synthesized by IDT was cloned into pLv-EF1a-IRES-puro (Addgene 85132), pAAV.TBG.PI.eGFP.WPRE.bGH (Addgene 105535) ...
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bioRxiv - Molecular Biology 2023Quote: A fragment containing an inducible TRE-tight promoter and a LAP tag (a FLAG-tag followed by an EmGFP-tag) was inserted at the XhoI site of the plasmid pBSKDB-CAG-rtTA2sM2-IRES-tSkid-IRES-Neo (Addgene #62346), thereby generating the vector pGEH_ind_LAP-C ...
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bioRxiv - Microbiology 2023Quote: HPV16 PsVs were produced by co-transfecting 293TT cells with wild-type p16SheLL-3XFLAG tag [14] together with pCAG-HcRed (Addgene #11152) or pCINeo-GFP [obtained from C ...
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bioRxiv - Biophysics 2023Quote: ... UblBilA was cloned into a vector encoding a C-terminal GFP tag (UC Berkeley Macrolab vector H6-msfGFP, Addgene ID 29725) and purified as above ...
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bioRxiv - Neuroscience 2024Quote: LexAop-Shv RNAi and LexAop-Shv fly lines were generated by subcloning Shv-RNAi hairpin sequence based on VDRC Shv-RNAi design or the coding regions of Shv which contains a V5 tag into the pJFRC19-13XLexAop2-IVS-myr::GFP vector (Addgene, 26224). Both lines were inserted into the X chromosome specific position by microinjecting the plasmid into a P{CaryP}attP18 fly line (BDSC ...
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bioRxiv - Biochemistry 2024Quote: Mutations of phosphorylation sites within the SR region were generated via site-directed mutagenesis based on either the tag-free SARS-CoV-2 Nucleocapsid protein plasmid (Addgene, #177937) or the Strep-tagged SARS-CoV-2 Nucleocapsid protein plasmid (Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... in which the TURBO-ID region was substituted by a 3HA-tag and the murine form of Sox11 (obtained from Addgene: #120387) was cloned after the tag (between restriction sites for EcoRI and BamHI) ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding the cytosolic domain of LjNFR5 fused to a Myc tag was cloned into plasmid pMAL-c2X (Addgene, 75286) between BamHI and HindIII and the NopM coding sequence was cloned into plasmid pACYCDuet (19 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with a plasmid expressing ACTN2 (NM_001103.4) with a eGFP tag.19 The plasmid was a gift from Johannes Hell (Addgene plasmid ref #52669). For immunoprecipitation of RBM20 ...
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bioRxiv - Molecular Biology 2024Quote: ... pL2M-CMV-MPH-P2A-Puro was cut using NheI-HF and BamHI-HF and the resulting vector backbone was used for Gibson Assembly with gBlock 5169 (see above) and a fragment containing p300Core-HA tag that was PCR amplified from pcDNA-dCas9-p300 (Addgene # 61357) using primers 5372/5373 ...
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bioRxiv - Genetics 2024Quote: ... with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-3xFLAG-empty-pgk-hph, Addgene, #48754) to create pPB-CAG-3xFLAG-HA-PATZ1-pgk-hph plasmid via Gibson assembly (NEB ...
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bioRxiv - Biochemistry 2024Quote: A pET30a plasmid containing the coding sequence for Pseudomonas aeruginosa succinyl-CoA:itaconate CoA transferase (PaIct) with a hexahistidine tag was purchased from Addgene (plasmid #111293)2 and transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... BubA was cloned into a vector encoding a C-terminal GFP tag (UC Berkeley Macrolab vector H6-msfGFP, Addgene ID 29725) and purified as above ...
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bioRxiv - Cell Biology 2022Quote: ... the cDNA sequences from LifeAct-GFP and LifeAct-RFP (Addgene) were inserted into our previously modified Drosophila expression vector driven by Slbo promoter ...
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bioRxiv - Immunology 2022Quote: ... The human TdT cDNA was cloned into CTV (Addgene #15912) construct in which the TdT expression was driven by CAG promotor and followed by a EGFP expression that mediated by an internal ribosome entry site (IRES ...
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bioRxiv - Immunology 2022Quote: ... The human TdT cDNA was cloned into CTV (Addgene #15912) construct in which the TdT expression was driven by CAG promotor and followed by a EGFP expression that mediated by an internal ribosome entry site (IRES ...
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bioRxiv - Neuroscience 2020Quote: ... was fused within the cDNA of (i) GluA1 (Addgene #24000) and of (ii ...
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bioRxiv - Immunology 2021Quote: The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene #102598) was used in combination with packaging vector pCL-Ampho (Novus ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid with YY1 cDNA (pcDNA3.1 HA-YY1, Addgene # 104395) was a gift from Richard Young47 ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA for hACE2 ectodomain was obtained from AddGene (#141185). The sequence was amplified using PCR with oligonucleotides hACE2ecto-Fw (5’-aaaatgatcaTCCACCATTGAGGAACAGGCC-3’ ...
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bioRxiv - Immunology 2020Quote: ... PD-L1 cDNA was cloned into pRRLSIN.cPPT.PGK vector (Addgene, 12252) with ClonExpress II One Step Cloning Kit (Vazyme ...
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bioRxiv - Neuroscience 2022Quote: Human DAT cDNA was amplified from YFP-hDAT (Addgene, 90228) and pHluorin was amplified from vGlut1-pHluorin (a gift from Timothy A ...
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bioRxiv - Molecular Biology 2022Quote: cDNA encoding RNaseH1-GFP was amplified from RED (Addgene # 139835) vector using the primers below ...
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bioRxiv - Cell Biology 2024Quote: Deaf1 cDNA was cloned into pAAV-CAG-GFP (Addgene, #37825), and Deaf1 shRNAs were cloned into pAAV-Ptet-RFP-shR-rtTA (Addgene ...
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bioRxiv - Biochemistry 2023Quote: The cDNA of MCU was cloned into pLYS5 (Addgene, #50054) with a FLAG tag at the C-terminus (6) ...
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bioRxiv - Biochemistry 2024Quote: ... ASS1 and SLC7A5 cDNA in the vectors plX304 (Addgene #25890) and plX307 (#41392) ...
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bioRxiv - Cancer Biology 2024Quote: The cDNA encoding human MED4 was obtained from Addgene (15425). The cDNA was sub-cloned in FLAG-HA-pcDNA3.1- (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA coding for human Dynamin1-K44A-GFP (Addgene #34681) was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: PACT cDNA was obtained from Addgene (pEN-TR4_PRKRA; plasmid # 106110). pLenti-PACT-HA was generated by inserting PACT cDNA into a modified pLenti vector containing a HA-tag in the N-terminal using the Gateway Cloning System (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... IDH2 cDNAs were cloned into retroviral backbone pMIG (Addgene #9044) as described (51) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting chimera genes for the light chain and the His-tagged heavy chain of the Fabs were separately cloned into the pCAGEN vector (a gift from Connie Cepko (Addgene plasmid #11160)) 34 ...
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bioRxiv - Biochemistry 2020Quote: ... coli RP hk339-GFP (monomeric His-tagged Kif5B kinesin motor domain) expression plasmid was a gift from Ron Vale (Addgene plasmid #24431).
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bioRxiv - Molecular Biology 2024Quote: The HTLV-1 CA (Gag amino acids 132-349) was cloned into the 6×His SUMO bacterial expression plasmid (pHYRSF53, Addgene, Watertown, MA), with mutants created by using the Gibson assembly procedure ...
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bioRxiv - Neuroscience 2023Quote: ... 2.3 x 1013 vg/mL) or 200 nL/side AAV5-CMV-HI-eGFP-Cre-WPRE-SV4 (Addgene; ≥ 1 x 1013 vg/mL) was injected at a rate of 100 nL/min into the insula of Pdynlox/lox and Oprk1lox/lox mice ...
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bioRxiv - Biochemistry 2024Quote: ... was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for bacterial expression of full-length human His-3C-HOIL-1 (pOPINB-HOIL-1 full-length) is available from Addgene (Plasmid #193858) (29) ...