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Citations for Addgene :
701 - 750 of 1569 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... (WT and variants) with a mTurquoise (mTq2) tag on the C-terminus were cloned into a pSBtet-pur vector (Addgene) using SfiI sites ...
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bioRxiv - Biochemistry 2023Quote: Restriction-free cloning 30 was used to generate the constructs of MglA-link-MglB with C-terminal hexahistidine tag in pHis17-KanR (mglA-link-mglB-H6, refer Addgene plasmid #78201 for vector backbone ...
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bioRxiv - Neuroscience 2023Quote: ... A virus lacking the hM4Di DREADD gene and instead containing the fluorescent tag eGFP (AAV8-CaMKIIa-EGFP, packaged by Addgene) was infused into ACC in 4 animals as a null virus control ...
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bioRxiv - Biochemistry 2023Quote: Phosphorylated tauC3 was expressed in Sf9 insect cells from a 438B pFastBac vector containing an engineered 6xHis tag and a TEV protease cleavage site (Addgene). Following two rounds of virus amplification ...
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bioRxiv - Microbiology 2023Quote: ... encoding the Spike glycoprotein of Wuhan SARS-CoV-2 fused to a C-terminal C9 tag (SW) was a kind gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Biophysics 2023Quote: ... human SLC26A9 (WT and missense mutants) with a C-terminally attached mTurquoise (mTq2) tag were cloned into a pSBtet-pur vector (Addgene) using SfiI sites ...
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bioRxiv - Cell Biology 2024Quote: ... The sequences were subsequently cloned in the destination vector MAC-tag-C, a gift from Markku Varjosalo (Liu et al., 2018) (Addgene plasmid # 108077 ...
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bioRxiv - Neuroscience 2024Quote: All cDNAs for human wild-type and mutant forms of CHCHD10 were synthesized and inserted in the pcDNA3 vector containing FLAG-tag by Genescripts and mCherryLC3B (#40827) plasmid was obtained from Addgene.
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bioRxiv - Developmental Biology 2024Quote: ... with a C-terminal TwinStrep-tag (IBA LifeScience) joined by a 3xGlyGlyGlySer linker was also inserted into the pHLsec vector (Addgene_99845) between AgeI and KpnI restriction sites ...
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bioRxiv - Developmental Biology 2024Quote: ... with a C-terminal TwinStrep-tag (IBA LifeScience) joined by a 3xGlyGlyGlySer linker was inserted into the pHLsec vector (Addgene_99845) between AgeI and KpnI restriction sites ...
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bioRxiv - Neuroscience 2024Quote: ... where a 6-residue histidine tag was inserted at the 587 aa position in the pAAV2-N587X plasmid (Addgene, #130877).
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bioRxiv - Cancer Biology 2024Quote: The wild-type and MARylation site mutated α-tubulin cDNA was amplified from pCDNA3 clones as previously described [12] using primers encoding an N-terminal FLAG epitope tag listed below and cloned into the pINDUCER20 lentiviral doxycycline (Dox)-inducible expression vector (Addgene, 44012; RRID: Addgene_44012).
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bioRxiv - Systems Biology 2022Quote: ... Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390 ...
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bioRxiv - Neuroscience 2020Quote: ... fused after to the GluA2 sequence (Addgene clone #24001, GluA2-pH) by replacing the pHluorin sequence ...
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bioRxiv - Cancer Biology 2021Quote: ... the two clones were recombined with pLx304 V5-DEST (Addgene #25890) using standard Clonase II LR mix (Thermofisher ...
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bioRxiv - Systems Biology 2023Quote: ... Entry clones were shuttled into LuTHy (Addgene #113446, #113447, #113448, #113449) and N2H (Addgene #125547 ...
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bioRxiv - Molecular Biology 2024Quote: ... we note that this clone (like the parental version at Addgene) lacks three amino acids residues (Phe Val Lys ...
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bioRxiv - Molecular Biology 2024Quote: ... BFP dest clone was a gift from Jacob Corn (Addgene #71825). Cells were then single-cell sorted into a 96-well plate for the top 2.5% fluorescent cells ...
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bioRxiv - Genetics 2021Quote: ... Putative enhancers were attached to a pes-10 minimal promoter::HIS-24::mCherry::let-858 3’UTR fragment amplified from POPTOP plasmid (71) (Addgene #34848) by using PCR stitching to create an enhancer reporter which was sequence verified and purified with a PureLink PCR purification kit (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... DNA encoding peptides derived from the C-terminal last 10 residues of the norovirus and cellular proteins were cloned into the pET-His-GST vector (Addgene:29655) with an N-terminal GST tag ...
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bioRxiv - Genetics 2022Quote: ... Enhancer reporters were produced by fusing via PCR stitching these constructs to a pes-10 minimal promoter::HIS-24::mCherry::let-858 3’UTR fragment amplified from the POPTOP plasmid [53] (Addgene #34848). The enhancer reporters were purified with a PureLink PCR purification kit (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... and 1x penicillin/streptomycin (pen/strep; PAA) and distributed in 6-well plates with HEK293 cells transfected with Neo1.a-AP-His (Addgene #71963) or pcDNA3.1-CNTN4-FLAG ...
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bioRxiv - Biophysics 2024Quote: The expression plasmid for HIS-tagged AavLEA1 was obtained from the Addgene plasmid repository [pET15b-AavLEA1 was a gift from Claude Férec (Addgene plasmid # 53093)]52 The expression plasmid for HIS-tagged RvLEAMshort was made by inserting a truncated cDNA from pEThT-RvLEAM49 [pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033)] ...
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bioRxiv - Molecular Biology 2024Quote: ... The lentiviral vectors pLU-CMV-His-ITCH and pLU-CMV-Flag-ITCH were generated by replacing TRAF6 coding sequences in pLU-CMV-TRAF6 (Addgene#66923) with a NheI/SalI-flanked sequence which was overlap PCR amplified from a cDNA library (HeLa ...
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bioRxiv - Cell Biology 2020Quote: P4M-GFP and mApple-Talin-N-10 were obtained from Addgene (51469 and 54951 respectively). FAPP-GFP was a gift from Tamas Balla ...
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bioRxiv - Cell Biology 2020Quote: ... mEmerald-N-Wasp-C-18 and mEmerald-Coronin1B from Michael Davidson (Addgene plasmids #54199, 54050), pmCherry-C1-WIP from Anna Huttenlocher ...
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bioRxiv - Cancer Biology 2020Quote: ... HPV16 E6 (p6661 MSCV-IP N-HA only 16E6 – Addgene plasmid # 42603 Dr. Peter Howley), HPV16 E7 (p6640 MSCV-P C-FlagHA 16E7-Kozak - Addgene plasmid # 35018 – Dr ...
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bioRxiv - Biochemistry 2021Quote: ... pRSF-1-NMT for expression of N-myristoyl transferase in bacteria was purchased from Addgene.
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bioRxiv - Neuroscience 2020Quote: ... AAV5-hsyn-DIO-rM3D(Gs)-mCherry (excitatory; titer: 1.3×1013 vg/mL; N=10 [Addgene, #50485 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 5 μg of mEmerald-Kinesin11-N-18 plasmid (Addgene number: 54137) 24 hours prior to imaging ...
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bioRxiv - Cancer Biology 2022Quote: SK-N-DZ cells were transduced with lentiviral particles encoding LRP8 gRNAs (lentiCRISPRv2 blast, Addgene plasmid #98293 was a gift from Brett Stringer) ...
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bioRxiv - Cell Biology 2022Quote: ... mEmerald Hc-SRC-N-18 (Src-eGFP here) was a gift from Michael Davidson (Addgene plasmid # 54118 ...
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bioRxiv - Microbiology 2021Quote: ... by flow cytometry on HEK293-FT cells transiently expressing SARS-CoV-2 N (Addgene # 141391), S (BEI # NR-52310 ...
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bioRxiv - Immunology 2022Quote: ... We first cloned the N- and C-termini of NFAT5 isoform A synthetized by Addgene into a pMSGV retroviral vector ...
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bioRxiv - Developmental Biology 2024Quote: ... The pDEST-CMV-N-Tandem-mCherry-EGFP plasmid was a gift from Robin Ketteler (Addgene plasmid #123216 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-EF1a-N-CretrcintG and AAV1-EF1a-C-CreintG were gifts from Connie Cepko (Addgene viral prep # 69570-AAV1 and 69571-AAV1 ...
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bioRxiv - Biophysics 2023Quote: ... or to the N-terminus of a glycosylphosphatidylinositol (GPI)-anchor (GPI-mEGFP, Addgene plasmid #182866) and were previously described [42 ...
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bioRxiv - Neuroscience 2024Quote: ... each 15 cm dish was transfected with 27.1 μg of SAD B19-N (Addgene #32630), 13.9 μg of SAD B19-P (Addgene #32631) ...
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bioRxiv - Bioengineering 2024Quote: ... Npu intein-split ABE constructs were cloned into N-and C-terminal AAV plasmids (Addgene plasmids 137177 and 137178 ...
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bioRxiv - Bioengineering 2024Quote: ... Npu intein-split ABE constructs were cloned into N- and C-terminal AAV plasmids (Addgene plasmids 137177 and 137178 ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse TRIM21 (Addgene #105516) and CRY2Clust (Park et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mouse Gqα15 (Addgene #40753) (Offermanns and Simon ...
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bioRxiv - Evolutionary Biology 2024Quote: ... mouse RIPK1 (Addgene #115341), and mouse RIPK3 (Addgene #78805 ...
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bioRxiv - Cancer Biology 2024Quote: ... pMJ179 (mouse U6; RRID:Addgene_85996), pMJ117 (human U6 ...
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bioRxiv - Molecular Biology 2020Quote: ... A C-terminal Strep tag on ORF24-NTD was added by inverse PCR to generate p6H-SUMO3-ORF24-NTD-Strep (Addgene #138467).
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bioRxiv - Molecular Biology 2020Quote: ... Full-length UL87 was PCR amplified from HCMV Towne BAC DNA with primers to introduced EcoRI and XhoI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag) using T4 DNA ligase (Addgene #138434). Full-length mu24 was PCR amplified from MHV68-infected 3T3 cell cDNA with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag ...
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bioRxiv - Molecular Biology 2020Quote: ... We built plasmids by Gibson assembly for constitutive expression of histones and K27M mutant histones tagged at their C-termini with 2XFLAG epitope tags using the Copia promoter from the plasmid pCoPURO (Addgene 17533), and Drosophila H3 and H3.3 histones from previously published constructs (Ahmad & Henikoff ...
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bioRxiv - Molecular Biology 2021Quote: ... The mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8xHis tag was obtained from Addgene (#145145). The mammalian expression vector for soluble ACE2 pcDNA3-sACE2 (WT)-sfGFP (#145171 ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the target proteins were dialyzed against PBS (pH 7.4) and the His6-SUMO-tag was removed by enzymatic cleavage using human SENP1 protease at 4°C overnight (Addgene #16356) (51) ...
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bioRxiv - Cell Biology 2021Quote: ... codon optimized coding sequences with a C-terminal HA epitope tag were synthesized by IDT and cloned into a pCW57.1 (Addgene plasmid #41393) derived lentiviral vector with a blasticidin resistance gene (replacing the original puromycin resistance gene) ...