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Citations for GenScript :
4051 - 4100 of 6194 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and synthesized by Genscript (Nanjing, CN). Plasmids were transformed into BL21 E ...
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bioRxiv - Cell Biology 2021Quote: ... Generation of pIRESneo2-EFGP-TOPBP1-W1145R is described elsewhere14 pIRESneo2-EGFP-TOPBP1-Δ813-892 was generated by cloning a fragment of TOPBP1 lacking aa 813 to aa 892 that was generated synthetically by Genscript (genscript.com) into pIRESneo2-EGFP-TOPBP1-WT by using SbfI and StuI restriction enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected with individual shRNA in pLKO.1 (Broad Institute, Cambridge, MA) mixed with packaging plasmid pCMV-dR8.91 (G193486, GenScript, Piscataway, NJ) and envelope plasmid pVSV-G (G178153 ...
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bioRxiv - Cell Biology 2021Quote: ... 25 μg protein from each sample was mixed with LDS Sample Buffer (M00676, GenScript) and loaded in each well of a 4-20% SurePAGE™ Bis-Tris gel (M00656 ...
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bioRxiv - Cell Biology 2021Quote: The genetically encoded pH indicator RpHiMEL was constructed and validated by GenScript. Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded in each well of a 4-20% SurePAGE™ Bis-Tris gel (M00656, GenScript). Proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (1620145 ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 (A00487, Genscript). All secondary antibodies were purchased from Invitrogen and used at 1:700 and were Alexa Fluor-488 Donkey anti-rabbit (A21206) ...
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bioRxiv - Cell Biology 2021Quote: KLC1D/E synthetic peptides used for CD or NMR measurements were purchased from Genscript (>98% purity). Sequences were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... this allowed the synthesis of a series of DNAs (Genscript) that could then be inserted between the newly created NheI site and the existing C-terminal EcoRI site ...
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bioRxiv - Cell Biology 2021Quote: ... was purchased from GenScript (ref. n° OHu13506). The Myc-mKCNJ2-T2A-IRES-tdTomato.lti (#978 ...
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bioRxiv - Immunology 2021Quote: ... and beta (1-244) chains were synthesized and cloned into the bacterial expression vector pET30a via NdeI and XhoI (Genscript, USA). Proteins were expressed as inclusion bodies in BL21 (DE3 ...
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bioRxiv - Immunology 2021Quote: ... Peptides were synthesized at >95% purity (Genscript, USA).
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bioRxiv - Immunology 2021Quote: ... Two TAP1 gRNAs (Genscript, USA) were introduced by lentiviral transduction ...
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bioRxiv - Cell Biology 2021Quote: ... and envelope plasmid pVSV-G (G178153, GenScript) at a 10:10:1 ratio using Lipofectamine™ 2000 according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: PopZ and derived mutant constructs for expression in human cells were generated through custom synthesis and subcloning into the pcDNA3.1+N-eGFP backbone by Genscript (Piscataway, USA). The mCherry-G3BP1 plasmid was a kind gift of Dr ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant GST-fusion proteins immobilized on glutathione-sepharose beads (GenScript) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-agarose or -sepharose (Sigma; GenScript) and Talon metal affinity resin (Clontech) ...
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bioRxiv - Cell Biology 2021Quote: ... cysteine terminated IKVAV and AG73 peptides (full sequences for IKVAV and AG73: CSRARKQAASIKVAVSADR, and CGGRKRLQVQLSIRT respectively, GenScript Biotech, Piscataway, NJ) were dissolved in 1X PBS pH 3.25 ...
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bioRxiv - Cell Biology 2021Quote: ... Chlamydomonas genes were chemically synthesized by GenScript (Nanjing,China) and inserted individually into vector plasmid pcDNA3.1(+)-N-HA or pcDNA3.1(+)-N-FLAG.
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bioRxiv - Cell Biology 2021Quote: ... which was synthesized to contain 7 modified TetO elements flanked by two minimal CMV promoter sequences based on pTet-T2 sequences (GenScript) (49) ...
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bioRxiv - Cell Biology 2021Quote: ... respectively (Genscript Biotech Corporation, NJ). The BamHI-NCH1-SbfI construct was sub-cloned by restriction cloning into an ECGFP donor vector containing eGFP-FLAG-HA (Gumber et al ...
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bioRxiv - Cell Biology 2021Quote: ... BamHI-mCherry-FLAG-HA-MKAKU41-BamH1 was synthesized by Genscript and cloned in pUC18 at the BamHI restriction site ...
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bioRxiv - Cell Biology 2021Quote: A synthetic and codon optimised PfAtg18 gene was purchased from GenScript as a pUC57-PfAtg18syn plasmid ...
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bioRxiv - Microbiology 2021Quote: ... the genes were commercially synthesized by Genscript and cloned into the pMMB67EH derivative ...
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bioRxiv - Cell Biology 2021Quote: ... and sequence verified by GenScript (Piscataway, NJ).
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bioRxiv - Cell Biology 2021Quote: ... CPD cDNA was from GenScript clone ID OHu10876 ...
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bioRxiv - Cell Biology 2021Quote: pGFP-Vps501YKAA was made commercially using site-directed mutagenesis (GenScript, Piscataway, NJ), introducing alanine mutations into following positions Y160A and K170A of pGFP-GPDVps501 ...
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bioRxiv - Cell Biology 2021Quote: Variants of the XFP11 peptide were chemically synthesized with >75% purity (Genscript, Piscataway, NJ). The amino acid sequences of the GFP11 peptides were ...
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bioRxiv - Cell Biology 2021Quote: WIPI2d10-364Δ263-295: ATG16L1 (207-230) complex was formed overnight with 5X molar excess peptide (GenScript). Crystals of the complex were grown using hanging drop vapor diffusion method at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... using a synthetic GLWamide neuropeptide (produced by Genscript; Lechable et al., 2020).
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bioRxiv - Neuroscience 2021Quote: ... monomers and LPS were detected using endotoxin detection kit following the manufacturer’s protocol (GenScript ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit) ...
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bioRxiv - Neuroscience 2021Quote: ... a designed convergent U6 and H1 promoters driven apposing piezo2-shRNA (or SC) expression cassette was synthesized (Genscript, Piscataway, NJ) and cloned into Mlul site of an expression plasmid in which EGFP was transcribed by a CMV promoter ...
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bioRxiv - Plant Biology 2021Quote: ... using a highly efficient wet protein transfer system (eBlot L1; GenScript, Nanjing, China). The membranes were blocked for 2 h at room temperature in TBST solution (2 mM This-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... The flg22 and pep1 peptides (GenScript; in mQ water) were administered at final concentrations of 100 nM and 200 nM ...
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bioRxiv - Plant Biology 2021Quote: The LeGPPS-RNAi (LeGPPSi) construct was created by synthesizing (Genscript, Piscataway, NJ) spliced fragments of the LeGPPS coding region corresponding to nucleotides 165-727 and 165-519 ...
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bioRxiv - Cell Biology 2021Quote: ... pcDNA-Gα13-Nluc and pcDNA-Gα15-Nluc were synthesized by GenScript. The subunits were then fused to cpVenus173 or Nluc at the indicated positions (Fig ...
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bioRxiv - Plant Biology 2021Quote: ... and custom synthesized (Genscript, Piscataway, NJ) (Supplementary Table S1) ...
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bioRxiv - Immunology 2021Quote: ... The synthetic transgene was codon optimized and engineered into the baculovirus vector (BV2373) for expression in Spodoptera frugiperda (Sf9) insect cells (GenScript, Piscataway, NJ, USA). NVX-CoV2373 spike trimers were detergent extracted from the plasma membrane with phosphate buffer containing TERGITOL NP-9 ...
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bioRxiv - Immunology 2021Quote: ... A blank consisting of the blocking buffer and a standard curve ranging from 5000 pg/mL to 78.25 pg/mL of S1 antigen (GenScript, Cat# Z03501) in blocking buffer were also added in duplicates on the plate followed by incubation at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: A monoclonal anti-SARS-CoV-2 RBD capture antibody (GenScript, Cat# 5B7D7) was coated on Nunc Maxisorp ELISA plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... biotinylated detection antibody (GenScript, Cat# 5E10G8-Biotin) was added at 1 µg/mL final concentration in blocking buffer and plate was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... An anti-SARS-CoV-2 Spike monoclonal neutralizing antibody (GenScript, Cat# 6D11F2) was used as a positive control ...
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bioRxiv - Immunology 2021Quote: ... One million of freshly isolated splenocytes were seeded into the precoated plates and stimulated with S1 and S2 peptides pools (GenScript) with a final concentration of 1 μg/ml of each peptide diluted in RPMI-1640 supplemented with 10% FBS and incubated for 48 hours at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid containing the full-length Arabidopsis thaliana Atm3 (AtAtm3) gene with a C-terminal 6x-His tag was purchased from Genscript (Genscript, NJ). Mutagenesis reactions generating the N-terminal 60 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant fraction was then incubated with 5 μg of the indicated antibodies and protein A-agarose beads (GenScript L00210) at 4°C on a nutator for 5 h ...
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bioRxiv - Microbiology 2021Quote: ... CSP-1 (GenScript, New Jersey, USA), as previously described (36) ...
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bioRxiv - Microbiology 2021Quote: ... ECD or those with desired ACE2 mutations were fused to the codon-optimized synthetic IgG1 Fc (GenScript) in which GASDALIE or LALA mutations were incorporated ...
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bioRxiv - Microbiology 2021Quote: ... a gene block was synthesized by GenScript (S12 Appendix), then assembled (as above ...
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bioRxiv - Microbiology 2021Quote: ... and JPS-G3 VHHs [20] separated by 15-amino acid flexible glycine-serine linkers ((GGGGS)3) was synthesized (GenScript Biotech, Piscataway, NJ) and ligated into pET32b(+ ...
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bioRxiv - Microbiology 2021Quote: ... N-terminus biotinylated peptides were synthesized by Genscript. N-terminal GSGS linker sequence was added to all peptide sequences ...