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Citations for GenScript :
751 - 800 of 915 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... derived from a peptide scan [15-mers with 11 amino acid overlap] through Spike glycoprotein of SARS-CoV-2) (JPT, Cat: PM-WCPV-5 or GenScript, Cat: RP30020). Phorbol Myristate Acetate (PMA ...
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bioRxiv - Biochemistry 2022Quote: ... or by adding 200 ng/well of monovalent or bivalent cAbCMY-2 (254) recognized by 1/2000 diluted rabbit anti-HCAbs antibody conjugated to HRP (Genscript, United States). TMB was used as substrate while reaction was stopped by 1 M H3PO4 ...
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bioRxiv - Immunology 2022Quote: ... and 1-3 x 105 cells were stimulated for 24-48 hours with 11 SARS-CoV-2 Spike peptide pools (17- or 18-mers with 11 amino acid overlap) (Genscript, Piscataway, NJ) at a concentration of 1μg/mL per peptide ...
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bioRxiv - Microbiology 2022Quote: ... Fifteen-mer overlapping peptides from SARS-CoV-2 spike glycoprotein (Cat# PM-WCPV-S-1, JPT peptides, Berlin, Germany) and MeV-nucleoprotein (Genscript, NJ, USA) were used to stimulate splenocytes at 5 µg/mL ...
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bioRxiv - Biophysics 2023Quote: ... WT mGluR 2 and mGluR3 peptides encompassing the first 23 residues of each CTD (mGluR2:PQKNVVSHRAPTSRFGSAAPRAS, mGluR3:PQKNVVTHRLHLNRFSVSGTGTT) were purchased from GenScript (Piscataway, NJ) (>= 95% purity) ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of cell lysate was saved as an input control and the remaining cell lysate was incubated with Glutathione MagBeads (GenScript, L00327) overnight at 4°C ...
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bioRxiv - Pathology 2022Quote: ... bat sera were screened at a 1:10 dilution using a competitive enzyme linked immunosorbent assay (SARS-CoV-2 sVNT, GenScript, Piscataway, New Jersey) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 nsp5 was cloned into the pGEX-6P-1 vector using BamHI and XhoI and then synthesized commercially (GenScript, Piscataway, NJ, USA). A native N-terminus is attained during expression through an nsp5 autoprocessing site corresponding to the cleavage between nsp4 and nsp5 in the viral polyprotein ...
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bioRxiv - Immunology 2023Quote: ... Sera were screened at a 1:10 dilution using a competitive enzyme linked immunosorbent assay with the S-RBD horseradish peroxidase (HRP) for Omicron BA.1 (SARS-CoV-2 sVNT L00847-A and S-RBD HRP Z03730, GenScript, Rijswijk, The Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... PLA2G7 and SIAE from Chinese hamster ovary (CHO) cells were expressed in CHO-3E7 cells and purified (>90% purity) by GenScript (Piscataway, NJ).
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently transfected with a custom plasmid (Genscript) expressing hsTRPA1 and dTom fluorescent reporter as for screening experiments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with mouse anti-HA antibody (Genscript) diluted 1:500 in PBS supplemented with 0.5% goat serum and 0.01% Tween-20 (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... cells and purified using the His6–affinity resin (GenScript). These proteins were labeled at the single Cys site (C459 ...
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bioRxiv - Cancer Biology 2020Quote: MC38 cells were transfected with the idAdpgkG plasmid (Genscript) using the piggyBAC vector/transposase system with Lipofectamine 2000 (Thermo ...
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bioRxiv - Immunology 2022Quote: ... cells were given 100 ng/ml SIITFEKL peptide (Genscript) for 3 hours.
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bioRxiv - Cancer Biology 2021Quote: ... the melanoma cells were spiked with gp100+ antigens (GenScript) just before addition of T-cells ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with pcDNA3.1-ELKS-Flag (Genscript), pEF1-K6a (no tag) ...
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bioRxiv - Microbiology 2022Quote: ... Mice from WT and CST-KO groups were injected intraperitoneally once daily with CST (2 μg/g body weight; Genscript Biotech Corporation, Piscataway, NJ) for 15 days based on previous experimentation 15 ...
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bioRxiv - Neuroscience 2023Quote: ... in the middle of exon 2 was conjugated to KLH to improve antigenicity of the peptide sequence followed by polyclonal antibody production in rabbits (Genscript, Inc. Piscataway, NJ, USA). Anti-Bdwf antibody was affinity purified against the antigenic peptide and specificity was confirmed by immunohistochemistry analyses.
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transduced with codon-optimized human ACE2 (Genscript) cloned into pBABE-puro [54] (Addgene) ...
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bioRxiv - Immunology 2019Quote: ... 1 × 106 cells were incubated with influenza Gp33 peptide (Genscript) at a final concentration of 05μg/mL in the presence of fluorescence-conjugated antibodies against CD107a and CD107b together with Brefeldin A for 5h before additional flow cytometric staining.
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bioRxiv - Bioengineering 2019Quote: ... Cell adhesive peptides (GRGDSPC, or scrambled GRDGSPC; >95% purity; GenScript) were dissolved in PBS containing 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK 293T cells were transfected with pLentiCRISPR V2 vectors (GenScript) expressing S ...
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bioRxiv - Cancer Biology 2023Quote: Cell lysates were separated on an SDS-polyacrylamide gel (Genscript) and transferred to a nitrocellulose membrane using a wet transfer system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The pcDNA3-SARS-CoV-2-Spike plasmid contains a codon- optimized ORF for Spike from GenBank NC_045512 that was synthesized by GenScript (a kind gift from David Kelvin) then cloned between the KpnI and BamHI sites of pcDNA3.1(+) ...
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bioRxiv - Pathology 2021Quote: ... Samples were mixed by vortexing and tested using the GenScript cPass™ SARS-CoV-2 Neutralization Antibody Detection Kit (GenScript USA, Inc. Piscataway, NJ, USA) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... OCRL1-/-(OCRL KO) HK2 and HEK293T cells were prepared by GenScript Inc ...
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bioRxiv - Biochemistry 2021Quote: RBD inhibition assay: HEK-293T cells stably expressing hACE2 (GenScript M00770) were seeded into a 24-well plate at an initial density of 6 x 104 cells per well ...
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bioRxiv - Microbiology 2019Quote: ... cstK coding sequence was also optimized for mammalian cell expression (GenScript), amplified by PCR and cloned into pDXA-3C (46 ...
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bioRxiv - Biochemistry 2019Quote: ... Surface expression was measured by incubating cells with 200uL TMB (Genscript) per well and reaction was stopped by transferring 100uL of developed colored solution to a 96-well plate already containing 100uL of 1M H2SO4 ...
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bioRxiv - Biochemistry 2020Quote: ... 1+/c-(k) - dyk expression vector for mammalian cells by GenScript Corporation (Piscataway ...
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bioRxiv - Genetics 2020Quote: ... cells were stimulated with increasing concentrations of agonist GIP (RP10795, GenScript) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... were reacted with equimolar cell-degradable peptide containing sequence KCGPQGIWGQCK (GenScript), 3.0 mM CRGDS basement membrane-mimicking peptide (GenScript) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with 2.5 μM TSKB20 (ANYKFTLV) peptide (Genscript Inc.) or 50 ng/mL PMA plus 1 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were codon-optimized for human cell expression (GenScript Codon Optimization Tool). Gene fragments were each cloned into a pαH eukaryotic expression vector with a C-terminal HRV 3C protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids used for mammalian cell over-expression were purchased from GenScript. NLuc-WT-CCNE1-3xFLAG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were pretreated with interleukin 4 (IL-4) (Cat. # Z02925-10, GenScript) at a concentration of 5 ng/ml for 24h ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:200 rabbit anti-S tag (Genscript A00625) overnight and 1:1000 mouse anti GFP (Thermo Fisher A-11120 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T stable transfected with ACE2 (GenScript M00770, 8000 cells per assay), Caco2 cells (40,000 cells per assay) ...
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bioRxiv - Cancer Biology 2021Quote: Peptides for spectra validation and T cell stimulation were synthesized by GenScript with >98% purity and TFA removal.
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bioRxiv - Immunology 2020Quote: ... cells were cultured in the presence of LCMV GP33-41 peptide (GenScript) and Protein Transport Inhibitor Cocktail (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... variable genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (gWiz or pcDNA3.4 ...
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bioRxiv - Microbiology 2020Quote: ... variable genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (gWiz or pcDNA3.4 ...
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bioRxiv - Systems Biology 2023Quote: ... B16 cells were pulsed for 0.5h with 5μg/ml OVA257-264 (Genscript), and then washed twice in RPMI ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain BL21 (DE3) cells and purified by Ni-NTA chromatography (GenScript).
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bioRxiv - Biophysics 2023Quote: ... Sf9 cell pellets with XPD expression were purchased from GenScript (NJ, USA). To purify XPD ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were separated by 4-20% SDS-PAGE (GenScript M00657) and transferred to a nitrocellulose membrane (0.45 µm ...
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Mammalian-unique eIF4E2 maintains GSK3β proline kinase activity to resist senescence against hypoxiabioRxiv - Cell Biology 2020Quote: ... HCT116 cells were cotransfected with plasmid pX330 and cas9 Nuclease (GenScript, Z03386-50) containing sgRNAs targeting exon 7 of eIF4E2-201 ...
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bioRxiv - Immunology 2022Quote: ... The cDNA sequence synthesized and cloned into pcDNA3.4 for mammalian cell expression (GenScript).
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bioRxiv - Genomics 2020Quote: Genomic DNA from the HEK293 cell line was purchased from GenScript (https://www.genscript.com).xs