Labshake search
Citations for Takara Bio :
401 - 450 of 808 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was directly ligated with pMD18-T vector (Takara) for TA cloning according to the pMD18-T kit protocol ...
-
bioRxiv - Systems Biology 2022Quote: ... viruses were purified using the Retro-X Concentrator kit (Clontech) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... purified using NucleoSpin Gel and PCR Clean-Up Kit (Takara), cloned into pRACE vector (provided in the kit ...
-
bioRxiv - Cancer Biology 2023Quote: ... Viral preparations were then purified by LentiX concentrator (Takara Bio). GSCs were infected with lentivirus and after 24 hours of incubation at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... protein complexes were purified from by Ni2+-NTA (Takara Bio) affinity chromatography (Dsl1:Qb:Qc and His7-Tip20:Sec20 ...
-
bioRxiv - Microbiology 2023Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
-
bioRxiv - Bioengineering 2024Quote: ... AAV was purified with the AAVpro Purification Kit (Takara, 6666) according to manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2022Quote: ... or a rabbit anti-DsRed polyclonal antibody (1:1000, Cat # 632496, RRID:AB_10013483, Takara Bio U.S.A., Inc., CA, U.S.A.). In addition ...
-
bioRxiv - Biochemistry 2022Quote: ... MBL) and HRP-labeled IgG detector (1/5,000 dilution from the product, Western BLoT Rapid Detect v2.0, Takara Bio) were used as primary and secondary antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
-
bioRxiv - Microbiology 2022Quote: ... Aer01 tagged with dTomato were labeled by incubating larvae in blocking solution containing Rabbit anti-dsRed (Takara Bio #6324960) at 1:500 dilution ...
-
bioRxiv - Genomics 2020Quote: ... and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson, Clontech [now Takara Bio USA], Mountain View, CA, USA). Sections were washed and stained with the secondary antibodies Streptavidin:Alexa Fluor 488 (Biolegend ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
-
bioRxiv - Microbiology 2020Quote: ... The amplicons were purified by DNA Amplification Clean Up Kit (Clontech). Amplicons were pooled in equimolar ratios prior to library preparation ...
-
bioRxiv - Microbiology 2020Quote: ... HIV viral particles were purified using the Lenti-X concentrator (Takara) and were resuspended in fresh DMEM medium supplemented with 5% of FBS ...
-
bioRxiv - Microbiology 2019Quote: ... the viral RNA was purified by NucleoSpin® RNA Kit (TAKARA). Recombinant INHis proteins (300 nM ...
-
bioRxiv - Biochemistry 2020Quote: ... CST was purified from the clarified lysate using Talon resin (Clontech) followed by protease cleavage of the GFP-His10 tag and finished with size exclusion chromatography on a Superdex 200 column equilibrated in Buffer A (25 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... coli BL21 and purified on 5 mL Talon column (Clontech®) loaded with Cobalt ...
-
bioRxiv - Immunology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Takara). Viral DNA was isolated from viral stocks by digestion with proteinase K (Roche ...
-
bioRxiv - Biophysics 2020Quote: ... The complex was then purified by binding on Talon resin (Clontech) and eluted in 150 mM imidazole ...
-
bioRxiv - Genomics 2019Quote: ... Purified PCR products were cloned into pMD18-T vector (TaKaRa, Japan) for transformation to DH5-α competence cells of Escherichia coli and sequencing.
-
bioRxiv - Developmental Biology 2019Quote: ... The BACs were purified with the nucleobond BAC 100 kit (Clontech). We co-injected 1 nl of 40 ng/μl Tol2 mRNA and 50–250 ng/μl of the cdh1:cdh1-sfGFP or cdh1:cdh1-TagRFP BAC transgene DNA into the lifting cell of the zygote of 0 to 20-minute old embryos ...
-
bioRxiv - Microbiology 2020Quote: ... Modified BACs were purified using a Nucleobond BAC 100 kit (Clontech). BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs) ...
-
bioRxiv - Cell Biology 2021Quote: ... purified nucleic acids were treated with DNA enzyme I (Takara, Japan). RNA was reverse transcribed using RevertAid reverse transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNAs were reverse-transcribed using PrimeScript reverse transcriptase (Takara) and primer 3 and then PCR-amplified using primer 3 and 4 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with I-Sce I into one-cell-stage zebrafish embryos ...
-
bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with 50 ng/μl Tol2 RNA into one-cell-stage zebrafish embryos ...
-
bioRxiv - Genetics 2022Quote: ... and then purified with RNase-Free DNase I (Takara Company, China). 1% agarose gel was used to evaluate the RNA contamination and degradation ...
-
bioRxiv - Neuroscience 2023Quote: ... were purified with the NucleoBond Xtra Midi EF kit (Takara 740420). Il6ra knockdown efficiency was assessed by transient transfection of sgRNA and dCas9-KRAB-MeCP2 into the mHypoA hypothalamic neural cell line (Cedarlane Labs ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting amplicons were PCR-purified (NucleoSpin® Extract II, Clontech). To clone the pool of oligonucleotides in the lentiCRISPRv2 vector ...
-
bioRxiv - Cell Biology 2023Quote: ... gel-purified (NucleoSpin Gel and PCR Clean-up kit from Takara) and the purified fragment was stored at -20°C ...
-
bioRxiv - Microbiology 2023Quote: ... HA trimers were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
-
bioRxiv - Plant Biology 2023Quote: ... and purified with Capturem™ His-Tagged Purification Maxiprep Kit (Takara). The binding reaction was performed in 20 μL binding buffer (10 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... We purified PCR products by agarose gel extraction (Takara Bio, 740609) and used BP clonase (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... was purified using the NucleoSpin RNA Clean-up kit (Takara Bio). Subsequently ...
-
bioRxiv - Genomics 2024Quote: ... Final libraries were purified with Nucleospin PCR Clean up kit (Takara) and quantified by Qubit HS (Thermo Scientific) ...
-
bioRxiv - Microbiology 2019Quote: ... and N-glycolylneuraminic acid (NeuGc) released were labeled with 1,2-diamino-4,5-methylenedioxybenzene (DMB) using a commercial kit (Takara, Shiga, Japan). The DMB-labeled sialic acids were analyzed by HPLC equipped with a TSK-ODS80Ts column (Tosoh ...
-
bioRxiv - Microbiology 2020Quote: ... The double-stranded oligonucleotides were end-labeled with [α-32P]-dCTP with the Random Primer Labeling Kit (Takara, Dalian, China). The binding reaction (28 μl ...
-
bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
-
bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
-
bioRxiv - Neuroscience 2019Quote: ... the slices were incubated overnight at 4°C with a rabbit polyclonal antibody against DsRed (1:1000, Takara Bio USA) diluted in a blocking solution of 0.3% Triton X-100 in PBS-DEPC ...
-
bioRxiv - Cell Biology 2022Quote: ... The transformants were screened by microscopically observing the GFP signals and further confirmed by a western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
-
bioRxiv - Cell Biology 2022Quote: ... and the presence of the kinA-GFP fusion was confirmed by western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
-
bioRxiv - Immunology 2021Quote: ... The following primary antibodies were used in this study: Living colors DsRed Polyclonal antibody (Rabbit, 1:300, Takara Bio #632496); FITC Anti-mouse Ki67 (1:100 ...
-
LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... membranes were incubated with epitope-specific rabbit polyclonal antisera or with an anti poly-histidine horseradish peroxidase-conjugated monoclonal antibody (TaKaRa). Immunodetection was revealed by using a Clarity Western ECL blotting substrate (BioRad ...
-
bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled primers were designed and the DNA fragment amplified from genomic DNA of HEK293T cells using Gflex DNA polymerase (Takara-bio). Amplicons were purified using NucleoSpin Gel and a PCR Clean-up kit (Takara-bio ...
-
bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked to membrane and hybridized with [γ- 32P] 5’-end labeled RFP-spacer specific probe (RFP_crRNA_probe, Supplementary Table 2) in ExpressHyb solution (Clontech Laboratories, Inc) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: Purified RNA was transcribed into cDNA using the PrimeScript Reagent Kit (Takara). The volume equivalent of 0.2 µg of RNA was reverse transcribed in a 40 µL reaction volume ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were purified using Capturem™ His-Tagged Purification kit (Takara Bio), then dialyzed by PBS buffer overnight ...