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Citations for Takara Bio :
551 - 600 of 808 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Zoology 2023Quote: ... The PCR products were purified using Nucleospin Gel and PCR Clean-up (Takara Bio). Nucleotide sequence analysis was outsourced from a commercial sequencing service (Eurofins ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified by gel electrophoresis and cloned into a T vector (Takara), and individual bacterial colonies were picked for sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... and then extracted and purified using a Nucleobond xtra maxi plus EF (Takara bio). Plasmids were linearized and fragmented by incubation with BsmBI overnight at 55 °C ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a permanent C-terminal His-tag were purified using TALON (Clontech) resin followed by anion exchange using a Hitrap Q column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... Purified PCR products were cloned into pYAO::hSpCas9 plasmid via In-Fusion (Takara, 639650).
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and purified using the NucleoSpin® Gel and PCR Clean-Up kit (TaKaRa, 740609), according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio). For immunocytokines ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... floating coronal sections were rinsed in PBS and blocked for 1–2 hr at room temperature in a solution of 10% normal goat serum and 0.5% Triton X-100 dissolved in PBS and then incubated in blocking solution containing rabbit anti-DsRed polyclonal antibody (1:1000; Takara Bio, Mountain View, CA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Biophysics 2020Quote: ... The double Cys mutants for EPR measurements were first purified using a His60 column (Clontech) followed by labeling with MTSL (1-oxyl-2,2,5,5 tetramethyl-Δ3-pyrroline-3-methylmethanethiosulfonate) ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, cat no. 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2020Quote: ... Both soluble and insoluble (cell pellet) fractions were purified via His-IDA nickel column (Clontech Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... DNA from each ChIP sample and the corresponding input sample was purified (PCR Cleanup, Takara) and analyzed further using qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Supercoiled pHSG299 plasmid dsDNA purified by CsCl-ethidium bromide gradient centrifugation was purchased from Takara Bio Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... The fragment amplified from Isoform000014 was purified and cloned to pMD-19T vector (Takara, China) followed by Sanger sequencing.
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bioRxiv - Cell Biology 2019Quote: ... Total RNA or purified mRNA was reverse transcribed with PrimeScript™ RT Master Mix (Takara) using Oligo dT primers to obtain complementary DNA ...
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bioRxiv - Genetics 2021Quote: ... The purified RNA (total 30 μg) was reverse transcribed using PrimeScript RT Master Mix (Takara). On-target or off-target sites of genomic DNA or cDNA were amplified with KOD Multi & Epi PCR kits (Toyobo ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant protein was purified using the His60 Ni gravity column purification kit (Takara Bio) according to the manual instruction ...
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bioRxiv - Biophysics 2020Quote: ... The viral particles were then purified using the Adeno-X Maxi Purification Kit (Takara Bio) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Finally the sgRNA was purified with Guide-it IVT RNA Clean-Up Kit (TaKaRa, Japan) and stored at −80□ for injection step.
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bioRxiv - Plant Biology 2019Quote: ... Isolated RNAs were purified using an RNA Clean-up Kit (TaKaRa Bio Inc., Tokyo, Japan). All kits were used according to the respective manufacturers’ protocols ...
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bioRxiv - Microbiology 2020Quote: ... to remove cellular debris and the protein was column-purified using anti-histidine resin (ClonTech). The resin was washed ten times with 10x column volumes of wash buffer mixed with an increasing proportion of tris buffer (20 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Purified AAVs were titered using a qPCR based kit (Cat. No. 6233; Takara Bio, USA). The TA muscle of each mouse received a 5 x 1010 vg of either AAV-GFP or AAV-CAAHR using several small volume (~7-8 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... and the purified products were sequenced directly at both ends with amplification primers by TaKaRa Biotechnology (Dalian ...
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bioRxiv - Immunology 2022Quote: ... Purified RNAs were reverse transcribed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, JP) and the S gene was amplified using PrimeSTAR GXL DNA Polymerase (Takara ...
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bioRxiv - Immunology 2022Quote: ... Purified RNAs were reverse transcribed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, JP) and the S gene was amplified using PrimeSTAR GXL DNA Polymerase (Takara ...
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bioRxiv - Microbiology 2022Quote: Purified plasmids were transfected into HeLa 229 cells using the Xfect transfection reagent (Takara Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Microbiology 2022Quote: ... The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio). Sex and age at sampling for the cells were as follows ...
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bioRxiv - Immunology 2022Quote: ... The reactions were pooled and purified with the Nucleospin gel and PCR purification kit (Takara). The purified PCR product was analyzed 2% agarose gel and subjected to sequencing on a HiSeq4000 (Fulgent Genetics) ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted and purified RNA was then converted to cDNA using PrimeScript cDNA synthesis kit (TaKaRa) according to the manufacture’s instruction ...
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bioRxiv - Genomics 2023Quote: ... purified RNA were constructed with the SMARTer Stranded RNA-seq Kit (Takara, cat. no. #634839) according to the manufacturer’s instructions for first- strand cDNA synthesis ...
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bioRxiv - Biophysics 2023Quote: WT and R203M N175-245were purified using the TALON His-tag purification protocol (Clontech Laboratories). Cell pellets were lysed in high salt buffer (50 mM tris ...
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bioRxiv - Cancer Biology 2022Quote: ... The ligation reaction was purified by ethanol precipitation and transformed into Stellar Competent Cells (Clontech) to assess library diversity by Sanger sequencing of 10 clones ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNAs that had been purified and concentrated using NucleoSpin RNA Clean-up XS (Takara Bio) were reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
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bioRxiv - Bioengineering 2023Quote: ... Proteins were purified from the soluble cell lysate using His60 Ni Superflow Resin (Takara Bio) and concentration was measured using NanoDrop ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). The guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L) ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Biochemistry 2023Quote: ... The secreted dystroglycan protein DAG128–749R311A/R312A was purified using His60 Ni IMAC resin (Takara) and subjected to anion exchange using DEAE resin ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was purified from N2 using Nucleospin RNA®(Takara Bio Inc., Kusatsu, Japan) as described in manufacture’s procedure ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Immunology 2024Quote: ... The virus particles were then purified using the Aneno-X Maxi Purification Kit (Takara Bio), and the buffer was replaced with 1X Formulation Buffer containing 2.5% glycerol (w/v) ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was purified using a NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) and eluted with 30 μl of 5 mM Tris-HCl buffer (pH 8.5) ...
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bioRxiv - Neuroscience 2021Quote: ... and the AAV was purified using AAVpro Purification Kit Maxi (All Serotypes) (no. 6666, Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were purified by Nucleospin® Gel and PCR Clean-up (740609-250; TAKARA).