Labshake search
Citations for Takara Bio :
101 - 150 of 808 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The cells were lysed by sonication then purified using immobilized metal affinity chromatography (IMAC) resin charged with cobalt (TALON resin; Takara). Proteins were eluted with 200 mM Imidazole in buffer containing 20 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
-
bioRxiv - Pathology 2023Quote: ... Affinity purification was performed at 4°C using Talon metal affinity resins (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
-
bioRxiv - Biophysics 2024Quote: ... The purification process involved an affinity chromatography step using cobalt affinity resin (TALON, Clontech), followed by a size exclusion chromatography (SEC ...
-
bioRxiv - Biophysics 2024Quote: ... MelBSt purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM ...
-
bioRxiv - Immunology 2022Quote: ... Talon cobalt affinity resin (Takara) was added to the treated supernatants and allowed to incubate for 15 min with gentle shaking ...
-
bioRxiv - Systems Biology 2020Quote: ... TALON cobalt affinity resin (Clontech) was used to purify the His-tagged protein using manufacturer’s protocols ...
-
bioRxiv - Neuroscience 2020Quote: ... Talon metal affinity resins (Clontech) beads were then added to purified protein mixtures as indicated ...
-
bioRxiv - Immunology 2020Quote: ... Talon cobalt affinity resin (Takara) was added to the treated supernatants and allowed to incubate for 15 minutes with gentle shaking ...
-
bioRxiv - Molecular Biology 2021Quote: ... Talon cobalt affinity resin (Takara) was added to the treated supernatants and allowed to incubate for 15 min with gentle shaking ...
-
bioRxiv - Biophysics 2019Quote: ... TALON Metal Affinity Resins (Clontech) equilibrated with normal buffer (25 mM sodium phosphate buffer (pH 7.6) ...
-
bioRxiv - Microbiology 2021Quote: ... Talon cobalt affinity resin (Takara) was added to the treated supernatants and allowed to incubate for 15 min with gentle shaking ...
-
bioRxiv - Biochemistry 2022Quote: ... TALON Metal Affinity Resin (Clontech); Mono Q 5/50 GL (GE Healthcare) ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2021Quote: ... The recombinant protein in the soluble fraction was then purified using TALON® Metal Affinity Resin (Clontech Laboratories, Mountain View, CA, USA) per the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... or TALON metal affinity resin (Takara), for GST-tagged and His-tagged constructs ...
-
bioRxiv - Cell Biology 2021Quote: ... and Talon metal affinity resin (Clontech), respectively ...
-
bioRxiv - Biochemistry 2021Quote: ... using TALON metal affinity resin (Takara) (Fridy et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... Cobalt-based affinity resins (Talon, Clontech) were used to capture target proteins with an N-terminal His-tag ...
-
bioRxiv - Plant Biology 2019Quote: ... and TALON® Metal Affinity Resin (Clontech), respectively ...
-
bioRxiv - Microbiology 2021Quote: ... Purification with TALON Metal Affinity Resin (Takara) was carried out from the insoluble fraction by using multiple rounds of sonication and washes in PBS containing 0.1% Tween (v/v) ...
-
bioRxiv - Biochemistry 2020Quote: ... Ni-NTA affinity resin was from Clontech. For anion exchange chromatography the HiTrapQ column was from GE Healthcare ...
-
bioRxiv - Biophysics 2021Quote: ... Talon metal affinity resin was from Takara Bio (Mountain View ...
-
bioRxiv - Microbiology 2023Quote: ... or TALON Metal Affinity Resin (Takara Bio) respectively ...
-
bioRxiv - Molecular Biology 2024Quote: TALON Metal Affinity Resin (TAKARA, Shiga, Japan) was used to purify N-terminal Hisx6-tagged TraR protein ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were purified under denaturing conditions by metal affinity chromatography using a column packed with 50% His60 Ni2+ Superflow Resin (Clontech, Mountain View, CA, USA). The purified effector proteins were dialysed (Spectra/Por®3 Dialysis Membrane ...
-
bioRxiv - Immunology 2023Quote: ... The 6-His tagged recombinant proteins were purified from the supernatant by gravity-fed through TALON® Metal Affinity Resin (Takara Bio, Shiga, Japan). Following a wash step with PBS (pH 8) ...
-
bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal (Clontech) and endogenous Bassoon was stained with 1:500 dilution of anti-Bassoon antibody ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting cell lysate was used for affinity purification and flowed over a TALON cobalt affinity resin (Takara Bio). The cobalt resin was washed with ~20 column volumes (CV ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
-
bioRxiv - Genetics 2019Quote: ... Lysis and Talon affinity resin purification (Takara Bio) and size exclusion chromatography was essentially as described [48] ...
-
bioRxiv - Biochemistry 2020Quote: ... and TALON® metal affinity resin from Clontech were used ...
-
bioRxiv - Cell Biology 2021Quote: ... TALON Metal Affinity Resin (Takara Bio, Catalog #635502) which binds His6-tagged proteins with high affinity ...
-
bioRxiv - Molecular Biology 2023Quote: 5 ml TALON metal affinity resin (TaKaRa Bio) was equilibrated with five column volumes of native lysis buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... The TALON Metal Affinity Resin (Takara, cat. 635501), Pierce Glutathione Agarose (Thermo ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
-
bioRxiv - Biochemistry 2024Quote: ... The recombinant SMC1A HD/RAD21C and SMC3 HD/RAD21N protein complexes were then purified by affinity chromatography using the 10xHis purification tag on RAD21 by incubating the cleared lysates with TALON Metal Affinity Resin (Takara Bio). The purification tag was then removed on the affinity beads by overnight 3C protease digestion at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were extracted from this crude lysate by affinity purification with TALON Affinity Resin (Takara Bio USA, Mountain View, CA, USA), washed with a solution containing 50 mM sodium phosphate ...
-
bioRxiv - Biochemistry 2019Quote: ... followed by affinity chromatography using Co Talon resin (Clontech). Further purification was accomplished by ion exchange and size-exclusion chromatography on an AKTA PURE system (GE Healthcare).
-
bioRxiv - Biochemistry 2019Quote: ... the supernatant was loaded on TALON affinity resin (Clontech), washed with buffer containing 10mM imidazole and eluted in buffer supplemented with 400mM imidazole ...
-
bioRxiv - Biophysics 2023Quote: ... Tandem affinity purification using TALON resin (Takara Bio Inc.) and Streptactin-XT resin (IBA Lifesciences ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
-
bioRxiv - Biophysics 2024Quote: ... TALON® Metal Affinity Resin was purchased from Takara Bio (Mountain View ...
-
bioRxiv - Plant Biology 2020Quote: The 5’ Digoxigenin labeled R-box and non-labeled oligonucleotides were synthesized (Takara, Dalian, China). The binding mixture contained nuclear extracts ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-dsRed rabbit polyclonal (Clontech Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit-anti-GFP (polyclonal, Takara) 1:5000 for denatured blots ...
-
bioRxiv - Molecular Biology 2021Quote: ... and loaded onto Co2+-charged TALON Metal Affinity Resin (Clontech) for 1.5 hours with agitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was incubated with Talon Metal Affinity resin (Clontech) for one hour or Glutathione S-sepharose 4B (GE Healthcare Life Sciences ...
-
bioRxiv - Plant Biology 2021Quote: ... and then applied to TALON metal affinity resin (Clontech, USA). The column was washed with buffer I (0.5 M NaCl ...