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Citations for Takara Bio :
601 - 650 of 964 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... for 24 h and transduced with lentiviral particles by spinfection (1000 x g for 90 min at 32°C) in the presence of Polybrene (5 μg/ml) on the plates coated with Retronectin (50 μg/ml) (Takara/Clontech) and anti-CD3 (1–2 μg/ml) ...
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bioRxiv - Biochemistry 2021Quote: ... Add 25 mL 200 g/L glucose and 25 mL 20 g/L amino acid drop-out mix (Clontech. Inc., Japan) solution to prepare the medium] ...
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bioRxiv - Synthetic Biology 2023Quote: ... Activated T cells were transduced on day 1 after stimulation using combinations of PEG-precipitated retroviral concentrates encoding different constructs adsorbed onto non-tissue culture treated 6-well plates coated with anti-CD3/CD28 2 μg/mL each and retronectin 20 μg/mL (Takara, T100B) at 1-2×106 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... the V3-V4 regions of the 16S rRNA gene were amplified using a 16S (V3–V4) Metagenomic Library Construction Kit for NGS (Takara Bio Inc, Kusatsu, Japan). The following primers were used (16S rRNA gene-specific sequences are underlined) ...
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bioRxiv - Genomics 2022Quote: ... For the Rubicon Genomics ThruPLEX® DNA-seq Kit: High Performance Library Preparation for Illumina® NGS Platforms (currently available from Takara Bio USA), the manufacturer’s protocol was followed.
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bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
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bioRxiv - Neuroscience 2023Quote: ... 12 cycles of amplification at PCR2 (final RNA-seq library amplification) and clean-up of final library using NucleoMag NGS Clean-up and Size Select beads (Takara Bio Inc., Shiga, Japan). Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, cat no. 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 2 μL of lysis buffer (0.2% Triton X-100, 4U of RNase inhibitor, Takara) per well ...
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bioRxiv - Genomics 2021Quote: ... These cells were cultured using Cellartis DEF-CS 100 Culture System (DEF-CS medium; Takara) under feeder-free condition ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were mixed with 100 μl of the Talon metal affinity resin (Clontech, USA) in a 1.5-ml tube ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The decanted soluble extract was adjusted to a volume of 90 mL with lysis buffer and incubated with 10 mL TALON cobalt resin (Takara, Shiga, Japan) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: BON1/HA-Bcl-xL/TR-shCtBP2 and BON1/HA-Bcl-xL/TR-shRLuc #713 cells were treated with 1 µg/ml (before sorting) or 0.5 µg/ml (after sorting) doxycycline (Clontech Laboratories, Inc., 631311) for 96 hours ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was loaded onto a 25 mL free-flow gravity column (GeneFlow) packed with 3 ml TALON® Metal Affinity Resin (Takara Bio), washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μg/ml Doxycycline (Clontech; Cat. No. 631311). iTF-iPSCs were counted and seeded onto double coated plates (Poly-D-Lysine-precoated Bio plates (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 ug/mL doxycycline (Clontech, Cat. No. 631311). i3Neurons were then fed three times a week by half media changes ...
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bioRxiv - Physiology 2021Quote: ... 1 mL of Fruit-mate (Takara, catalog no.: 9192) was added ...
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bioRxiv - Microbiology 2022Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat# 635683) column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set ...
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bioRxiv - Neuroscience 2021Quote: ... were coated with 9ug/mL retronectin (Takara bio T110B) and incubated overnight at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A) at 37°C for 1 h in 10 µl of a reaction mixture containing 20 mM HEPES–KOH pH 7.5(Nacalai 15639-84) ...
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bioRxiv - Microbiology 2021Quote: ... followed by selection with 2 μg/ml puromycin (Clontech). (For the sequences of shSIRT6 ...
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bioRxiv - Microbiology 2021Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat # 635683) column was equilibrated with 10 column volumes of Equilibration Buffer (HisTALON™ Buffer Set ...
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bioRxiv - Immunology 2021Quote: ... culture plates were coated with RetroNectin (20μg/ml) (Takara) at 4o C ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of lung tissue RNA (Takara Bio, 636524) at a final concentration of 500 pg per μl was used ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 ml of Tractor Buffer (Cat# 63567, TAKARA), followed by 2.5U/ml of DNase I and 50 mg/ml of lysozyme were added and incubated for 10 min at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Neuroscience 2023Quote: ... with 2µg/mL doxycycline (Takara Bio, Cat. No. 631311) to induce expression of NGN2 ...
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bioRxiv - Developmental Biology 2024Quote: ... 3.3 mL of Lenti-X concentrator (Takara, cat.# 631231) were added to 10 mL of filtered SN ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Neuroscience 2024Quote: ... Protamine sulfate (Final concentration: 8 μg/ml, Takara Bio) was added ...
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bioRxiv - Genetics 2023Quote: ... mixed with 5 mL Lenti-X Concentrator (Takara, 631232) and rocked at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... with a total of 1 µg of good quality total RNA as input and enriched using the SMARTer Apollo NGS library prep system (Takara Bio USA, Mountain View, CA). Sequencing libraries were prepared using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 ng RNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Europe SAS). Libraries were prepared from the purified cDNA using the Nextera™ XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2 μL of PBS including 0.2% Triton X-100 and 4U of RNase inhibitor (Takara) per well ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3 μl of lysis buffer (0.13% Triton-X-100, 4 units of recombinant RNase Inhibitor, Takara) was added to 3.7 μl of cell-free CSF and plasma ...
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bioRxiv - Developmental Biology 2020Quote: ... dissected calvaria from 9SL or 11SL fish were labeled with antibodies against mCherry (Takara, 1/100), GFP (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were split in two and passed through two CHROMA SPIN+TE-100 columns (Takara 636072), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... and 500 ng total RNA was incorporated for first-strand cDNA synthesis using the M-MLV reverse transcriptase kit (TaKaRa Bio USA, Mountain View, CA, USA), and the library was used as a template for qRT-PCR analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... and cDNA was synthesized using 1500 ng of total RNA and random hexamers (20ng/μL) with the SMART® MMLV as per manufacturer’s instructions (Takara Bio USA, Mountain view, CA, USA). cDNAs were used in PCRs with PLRV specific primers (F-5’ATGAGTACGGTCGTGGTT-3’ and R-5‘CTATTTGGGGTTTTGCAAAGC-3’) ...
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bioRxiv - Evolutionary Biology 2023Quote: We prepared each sequencing library from 1 μg of total RNA using a PrepX PolyA mRNA Isolation Kit (Wafergen, 40047) on the Apollo 324 NGS Library Prep System (Takara Bio USA Holdings, Mountain View, CA). We added Illumina indices and amplified libraries with 12 cycles of PCR and cleaned them using PCRClean DX beads (Aline Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Clarified lysates were loaded onto a 1 mL TALON (Takara) column ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 U/mL DNase I (2212, Takara, Dalian, China) at 37 °C for 10 min with intermittent agitation every 5 min ...
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bioRxiv - Microbiology 2019Quote: ... cells were treated with 1 µg/mL of doxycycline (Clontech) for 3–5 days.
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was nutated with 1 mL TALON resin (Takara) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1% PS and doxycycline (1 μg/ml; Takara, Cat# 1311N). At 7 days posttransfection ...
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bioRxiv - Microbiology 2022Quote: ... 1% PS and doxycycline (1 μg/ml; Takara, Cat# 1311N). At six days posttransfection ...