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Citations for Takara Bio :
551 - 600 of 964 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the cDNA samples were synthesized from 500 to 1000 ng total RNA using the PrimeScriptTM RT Master Mix cDNA (Takara, Kyoto, JAP) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from organoids using Nucleo Spin (MACHEREY NAGEL) and cDNA was synthesized from 500 ng of total RNA using PrimeScript™ RT Master Mix (TAKARA BIO). We performed quantitative reverse-transcription PCR (qRT-PCR ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed with 200 ng total RNA using the Prime Script RT reagent Kit with gDNA Eraser (Takara, Dalian, P.R.China) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The Apollo 324 NGS Library Prep System was used with the PrepX RNA-seq library preparation kit (Takara Bio, San Jose, CA). Sequencing was performed on Illumina NovaSeq 6000 platform (SP 100nt Lane ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using the directional PrepX RNA-seq library preparation protocol on the automated Apollo 324TM NGS Library Prep System (Takara Bio, CA), with a cDNA insert average size of ∼200bp.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and resultant RNAs (10 ng per sample) were subjected to cDNA library construction (SMARTer Mouse TCR a/b profiling kit, Takara Bio, Japan). The next generation sequencing was performed with MiSeq (Illumina ...
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bioRxiv - Immunology 2023Quote: ... 110 ng of RNA were retrotranscribed in a final volume of 10 μL using the PrimeScript RT reagent Kit (Takara, Kusatsu, Japan) with a combination of oligo-d(T ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was synthesized from 400 ng of DNase-treated total RNA by the Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Immunology 2019Quote: ... Non-tissue culture treated 24-well plates were coated with 0.5 mL per well of 10 μg/mL RetroNectin (Takara) on day 1 and stored overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: About 130 ml of clarified cell suspension was combined with 3 mL of TALON® resin (Takara Bio 635503) previously equilibrated in lysis buffer and rocked at 4°C for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Retroviral supernatant (5 ml) was added to non-treated retronectin-coated (10 µg/ml) 6-well plates (Takara Bio) and incubated for 4 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... thlaspeos tissue was resuspended in 9 ml protoplasting buffer supplemented with 10 mg/ml Yatalase (Takara Bio, Kusatsu, Japan) and 20 mg/ml Glucanex (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was re-suspended in four millilitres of filtered-sterilized (0.22 µm filter) lysing buffer (75 mg/mL BSA; 10 mg/mL Yatalase (Takara); 5 mg/mL Lysing Enzymes (L1412 Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... A thousand ng of total RNAs were used for cDNA synthesis using the SMART MMLV Reverse Transcriptase (Takara Bio, Mountain View, CA, USA). For RT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... total RNAs (100–300 ng) extracted from sex determined adults or pharate larvae (108 hpo) were reverse-transcribed using PrimeScript™ II Reverse Transcriptase (TaKaRa Bio Inc.) at 30°C for 10 min ...
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bioRxiv - Genetics 2019Quote: ... and performed the 2nd round of PCR to attach to the libraries adaptor and index sequences for the NGS analysis for 8 cycles again with Tks Gflex™ DNA Polymerase (Takara, Cat#R060A). The DNA sequences of the adaptor/index primers are listed in Table S6 ...
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bioRxiv - Genetics 2019Quote: ... then converted to Illumina sequencing library using the PrepX RNA-seq library preparation protocol on the automated Apollo 324™ NGS Library Prep System (Takara Bio, CA). Basically ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was reverse transcribed from 500 ng of total RNA with hexamers using a PrimeScript RT reagent kit (Takara Bio, Inc., Kusatsu, Japan). qPCR was then performed in a 25 μl reaction using TB Green Premix Ex Taq II reagent (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Biochemistry 2023Quote: ... 20 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio USA. Inc), which converts poly(A ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal quantities (5 ng) of total RNA from each sample were used for cDNA synthesis using the PrimeScript®RT reagent kit (TaKaRa, Tokyo, Japan). The reverse transcriptions of miRNAs were performed using looped miRNA-specific RT primers for miRNAs ...
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bioRxiv - Molecular Biology 2021Quote: Slide embedded tissue sections were incubated in PBS containing 0.2 mg/ml saponin (Nacalai teques) and 10 μg/ml Proteinase K (Takara Bio) for 20 min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... transfected cells were treated with 200 µg/mL hygromycin B (Nacalai Tesque, Kyoto, Japan) or 1 µg/mL puromycin (Clontech) for the selection of stably transfected cells.
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bioRxiv - Immunology 2020Quote: ... for 24 h and transduced with lentiviral particles by spinfection (1000 x g for 90 min at 32°C) in the presence of Polybrene (5 μg/ml) on the plates coated with Retronectin (50 μg/ml) (Takara/Clontech) and anti-CD3 (1–2 μg/ml) ...
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bioRxiv - Molecular Biology 2022Quote: ... the NaCl concentration of the cleared lysate was adjusted to 500 mM and the cleared lysate was applied to 10 mL (=5 mL bed volume) equilibrated Talon SuperFlow Metal Affinity Resin (TaKaRa) per L culture on ice for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 4×106 B cells per well were plated in 2 mL on 6-well plates that had been coated with RetroNectin (25 μg/mL, 4°C, overnight; #T100B, Takara), blocked with 2% BSA in PBS (1h ...
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bioRxiv - Neuroscience 2020Quote: ... Transplanted hiOLs were identified using anti-human cytoplasm (STEM121; Takara, Y40410, IgG1, 1:100), anti-human nuclei (STEM101 ...
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bioRxiv - Microbiology 2019Quote: ... and then prehybridized in 5 mL ExpressHyb (ClonTech) for 1 hour at 65°C ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 2 µg/ml doxycycline (Clontech, 631311) for 3 days prior to electroporation and to induce Cas9 nickase expression ...
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bioRxiv - Synthetic Biology 2019Quote: ... and rapalog (0.6mg/mL, Takara Cat. No. 635055) were dissolved into vehicle solutions containing 16.7% propanediol ...
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bioRxiv - Cell Biology 2020Quote: ... and doxycycline (1 μg/ml, 631311; Clontech Laboratories) were used for drug treatments.
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bioRxiv - Developmental Biology 2020Quote: ... and once in 5 ml NDiff227 (Takara Y40002). mESCs were then pelleted by centrifugation for 5’ at 1000rpm and resuspended in 500µl of NDiff227 ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of Lenti-X concentrator (Takara Bio) was added to 12 ml of cell supernatant and the mixture incubated at 4°C with gentle agitation for 18 hours ...
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bioRxiv - Molecular Biology 2023Quote: 5 ml TALON metal affinity resin (TaKaRa Bio) was equilibrated with five column volumes of native lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The virus titer (ifu/ml) defined by Clontech’s Lenti-X qRT-PCR Titration Kit (Cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 250 μg/mL proteinase K (Takara, Dalian), incubated at 55 °C for 1h ...
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bioRxiv - Immunology 2023Quote: ... previously coated with Retronectin (25 µg/ml) (Takara) by combining viral supernatant and activated splenocytes and spinning for 1.5 h at 2000 RPM ...
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bioRxiv - Cell Biology 2023Quote: ... added with 0.8 ml pure TALON IMAC (Clontech) resin and 20 mM imidazole ...
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bioRxiv - Bioengineering 2022Quote: ... 800 μg/mL hygromycin (Clontech, San Jose, CA) was used for the selection ...
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bioRxiv - Systems Biology 2023Quote: ... 50 μL of 20 mg/mL BSA (TaKaRa), 500 μL of 20% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μg/ml doxycycline (Clontech, cat. no. 631311), 1X N2 supplement (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and 10 μg/ml RetroNectin (Takara, Cat# T100A). Then ...
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bioRxiv - Biochemistry 2020Quote: Inducible K562 cells were plated at 2.5×105 cells mL−1 in RPMI 1640 containing 0.25 μg/mL doxycycline (dox) (Takara Bio USA Inc.) in 10 cm plates and incubated at 95% humidity ...