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Citations for Takara Bio :
501 - 550 of 964 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... a 659-bp DNA fragment of DsRed2 was amplified from 1 ng of pDsRed2-N1 plasmid (Clontech, Mountain View, CA, USA) with primers (5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′ and 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′) ...
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bioRxiv - Systems Biology 2020Quote: ... cDNA libraries were synthesized with 4 ng of total RNA input to the Clontech SMARTer smRNA-seq kit (Takara Bio 635034) using the manufacturer’s suggested protocol ...
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bioRxiv - Biochemistry 2021Quote: The proS gene was amplified from 50 ng population genomic DNA in a 25 μL PCR reaction using the high-fidelity polymerase PrimeSTAR Max DNA Polymerase (Takara Bio) and primers 134 and 138 (Supplementary Table 7) ...
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bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and reverse transcription and cDNA amplification were performed using 10 ng of RNA using the SMARTer Ultra Low RNA kit (Clontech Laboratories) like previously reported 13 ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 10 ng DNA from the ε2/ε2 and ε3/ε3 cell lines with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
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bioRxiv - Genomics 2022Quote: ... The ligation reactions were heat-inactivated at 65°C for 10 minutes and cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio) before electroporation into Endura Electrocompetent E ...
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bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... Ten ng total RNA was used as template for SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian (Takara Bio.) following the manufactureŕs recommendation.
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bioRxiv - Microbiology 2022Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... total RNA (1 ng) was injected into the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Palo Alto, CA, USA) for sequencing ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... RNA (1 ng) was used for cDNA synthesis and amplification using the SMART-Seq HT RNA-Seq library amplification kit (Takara Bio). According to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... Up to 30 ng of post-Immunoprecipitation dsDNA as measured by Invitrogen™ Qubit™ highsensitivity spectrofluorometric measurement was made into Illumina-compatible NGS libraries using the ThruPLEX® DNA-Seq kit from Takara Bio Inc ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from these retained samples was reverse-transcribed into cDNA using 250 ng as a template and the PrimeScriptTM RT Master mix (#RR036A, Takara, France). To provide a comparison point for the allele-specific expression in F1 larvae ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP 61,62 (400ng) using TransIT-LT1 (Takara, Cat# MIR2306). On day 3 (24 hours post transfection) ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% NGS: rabbit anti-ZsGreen (1/1000, Clontech, 632474, RRID:AB_2491179) or rabbit anti- DCX (1/2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the total RNA was used for cDNA synthesis using the Prime Script 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A). Random 6-mer primers (0.3 µL ...
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bioRxiv - Cell Biology 2023Quote: ... A quantity of RNA in the range 200 – 500 ng was used for cDNA synthesis using the PrimeScript RT-PCR Kit (Takara-Bio). Quantitative PCR was then performed using Taqman Fast Advanced MasterMix with Universal Probe Library Probes (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries are prepared by incorporating 1-10 ng of cfDNA with the ThruPLEX® Plasma-seq Kit by Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara ...
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bioRxiv - Neuroscience 2024Quote: Total RNA (500 ng) was reverse transcribed to cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, RR047A). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix kit and detected by QuantStudio3 system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Genomics 2019Quote: ... 10 U/ml DNase I (TaKaRa 2270A) was added to the reaction after 3-min digestion and gently pipetting was repeated every 5-6 min digestion ...
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bioRxiv - Neuroscience 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1ug/ml Doxycycline (Dox) (TAKARA Bio), and 100ug/ml penicillin-streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Genomics 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Neuroscience 2023Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2U/ml Recombinant ribonuclease inhibitor (Takara, #2313B). A second centrifugation (same conditions ...
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bioRxiv - Neuroscience 2023Quote: ... 40U/mL of RNase inhibitor (Takara: 2313B)] ...
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bioRxiv - Cell Biology 2023Quote: ... doxycycline (0.03 µg/mL, 24 hours; TAKARA) and Biotin (500 µM ...
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bioRxiv - Immunology 2024Quote: ... Retronectin-coated (overnight, 12 µg/mL, Takara) Non Tissue Culture flat bottom plates (24 well ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Plates are fixed in 4%PFA ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930 ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Plant Biology 2019Quote: ... and 0.6 μl of 100 μM random primer (N)6 (TaKaRa, Kusatsu, Japan), with RNase-free water added up to 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... The BAC16 ORF20 mutant was purified using the NucleoBond BAC 100 kit (Clontech). iSLK cell lines latently infected with the KSHV ORF20STOP virus were then established by coculture ...
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bioRxiv - Cell Biology 2022Quote: ... FKBP-PKR was activated with 100 nM of the homodimerization ligand AP20187 (Takara), as previously described(Zappa et al ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... and the radioactive probes were purified using CHROMA SPIN+TE-100 columns (Clontech). Membranes were prehybridized in Rapid-hyb Buffer (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto 100 μL TALON metal affinity resin (Takara Bio) pre-equilibrated with TBS pH 8.0 containing 10 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Complementary DNA (cDNA) was generated from previously extracted total RNA (900 ng) using the SMARTER RACE 5’/3’ kit (Takara Bio USA) according to the manufacturer’s instructions ...