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Citations for Takara Bio :
451 - 500 of 964 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The sequence spanning the I-SceI cleavage site in the SceGFP gene was amplified from 150 ng of genomic DNA using Advantage 2 Polymerase (Clontech) with primers dr-for (5’-CCCGCCACCTGCCCCATCTGCTA ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized from 10 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We amplified a 1.4 kb genomic fragment with PCR primers AIP3F (5’- GGCGCTATACCCGCTCGTGTCC-3’) and AIP5R2 (5’-CTTCATATTTGAAGACGAGGGAGG-3’) using 10 ng genomic DNA and Advantage DNA polymerase (Clontech) with the following cycling conditions ...
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bioRxiv - Genomics 2023Quote: ... 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Afterwards an average of 10 fmol of amplified cDNA was used to prepare library following SQK-PBK004 kit (PCR Barcoding kit ...
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bioRxiv - Genetics 2023Quote: ... cDNA was generated from 2 ng of RNA using the Smart-Sq V4 Ultra Low Input RNA Kit (Takara, 634894). Next ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated from 5 ng of RNA with the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio (#634889 ...
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bioRxiv - Microbiology 2023Quote: ... siRNA Knockdown efficiency was tested as follows: 200 ng of RNA was reverse transcribed with Prime Script RT Master Mix (Takara) and quantified using QuantiFast SYBR Green RT-PCR Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1%Triton X) and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). For the Sera-Mag™ Select (29343052 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl/well of beads were washed twice with TE solution and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... The sequencing reads were aligned to the mm10 genome using the Cogent NGS Analysis Pipeline (CogentAP, Takara Bio, v.1.5.1). Gene-barcode count matrices were then analyzed using R (v.4.0.0 ...
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bioRxiv - Immunology 2023Quote: ... total RNA (0.5 ng) was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Immunology 2024Quote: ... 1 ng RNA was used as input to generate cDNA with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and 5 mg/mL Doxycycline (Clontech).
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bioRxiv - Cell Biology 2020Quote: ... 0.125 μg/mL aureobasidin A (Takara), 50 μM cerulenin (Cayman) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 µg/mL of puromycin (Clontech) was added to cells for 2 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... and doxycycline (2 mg/ml, Clontech). After 6 hours ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µg/mL doxycycline (Clontech # 631311), 40 nM Antimycin (Sigma-Aldrich # A8674) ...
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bioRxiv - Cell Biology 2021Quote: ... containing doxycycline (2 mg/ml, Clontech). We kept the cells in this medium for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL RNAiso Plus reagent (TakaRa) was added to each bacterial cell pellet and mixed vigorously by pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/ml of puromycin (Takara) was added to the medium only for two days.
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
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bioRxiv - Immunology 2024Quote: ... of 25 μg/mL Retronectin (Takara) in PBS and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... doxycycline (Dox) (Takara, 0.2 μg/ml), pan-caspase inhibitor Q-VD-Oph (QVD ...
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bioRxiv - Cell Biology 2019Quote: ... Unincorporated UTPs were removed using CHROMA SPIN-100-DEPC-H2O columns (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Up to 100 cells/subset were collected in lysis buffer (Takara Clontech) containing RNAse inhibitors ...
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bioRxiv - Immunology 2019Quote: ... Up to 100 cells/subset were collected in lysis buffer (Takara Clontech) containing RNAse inhibitors ...
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bioRxiv - Genomics 2019Quote: ... We concentrated the virus 100-fold with Lenti-X concentrator (Clontech, 631231) following the manufacturer’s recommendations before aliquoting and freezing at -80C.
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bioRxiv - Molecular Biology 2019Quote: ... The modified BACs were purified using a Nucleobond BAC 100 kit (Clontech). BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... The modified BACs were purified using the NucleoBond BAC 100 kit (Clontech).
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bioRxiv - Cell Biology 2019Quote: ... Lentiviral supernatants were concentrated 100-fold before use (Lenti-X concentrator, Clontech). Concentrated virus was used for transducing primary fibroblasts and hTERT-RPE1 cells.
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bioRxiv - Molecular Biology 2019Quote: ... 100 nL lysis mix was added (0.8 U RNase inhibitor (Clontech, 2313A), 0.07% Igepal ...
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bioRxiv - Genomics 2019Quote: ... 10-U/μL SMARTScribe™ Reverse Transcriptase (100 U/μL; Clontech, 634936), and 3.2 μL of Polaris RT Plus Reagent (Fluidigm ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM maleic acid buffer pH 5.5) containing 100 mg Yatalase (Takara) and 100 mg Lysing Enzymes from Trichoderma harzianum (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was bound to 3 mL (=1.5 mL bed volume) Talon SuperFlow Metal Affinity Resin (TaKaRa) per protein preparation ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 ng of RNA was used for cDNA synthesis (SMART-Seq v4 Ultra Low Input RNA kit – Takara Bio: #634898). Verification of library quality and sequencing were done as for the embryonic dataset (see above) ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Full-length RNA-seq libraries from CM and skin macrophages were prepared from ∼3 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) followed by Nextera XT protocol (Illumina) ...
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bioRxiv - Genomics 2020Quote: ... and the resulting DNase I-treated RNA (~2 ng) was processed for sequencing by using a SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Biophysics 2019Quote: ... NG-Scarlet and NG-Cherry were created by deleting mRuby3 from NG-Ruby3 by inverse PCR and insertion of either mScarlet-I or mCherry using In-Fusion cloning (Takara Bio). NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was generated from 10 ng of RNA using the SMART-Seq v4 UltraLow Input RNA Prep kit (Takara Bio Inc) per manufacturer’s suggestions ...
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bioRxiv - Physiology 2021Quote: ... 10 ng of obtained RNA was processed using the SMARTer Stranded RNA-seq kit for Illumina (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... PCR product was gel-purified and 400 ng of the purified product were used in a random priming reaction containing: 4 Units Klenow fragment (TAKARA, Japan), 5 μl 10x Klenow reaction buffer ...
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared from 10 ng total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of total RNA was used for the cDNA synthesis reaction using PrimeScript™ RT Master Mix kit (TaKaRa RR036A) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... 5 ng of total RNA was used with the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TAKARA, Japan), according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.5 μL Porcine Transmissible Gastroenteritis and Porcine Epidemic Diarrhea Vaccine (Strain WH-1R + Strain AJ1102-R, Wuhan Keqian Biological. Company, Ltd.) or 1 ng λDNA (3010, Takara, Japan) were suspended and mixed together using 1.5 mL of sample preservation medium (R503 ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of RNA samples (200 ng) were used to generate complementary DNA using a PrimeScript RT reagent kit (TaKaRa Bio) with oligo-dT and random primers ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Molecular Biology 2021Quote: ... One ng of DNA was used for real-time qPCR analysis using Premix Ex Taq DNA polymerase (Takara, cat. no. RR039W) on ABI Fast 7500 (Applied Biosystems® ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was reverse-transcribed from 800 ng of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Gene-specific primers with appropriate amplification efficiency (0.95–1.05 ...