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Citations for Takara Bio :
401 - 450 of 1295 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Full-length and truncated Cntn1 was PCR amplified from rat contactin-myc 34 and ligated together using an In-Fusion Snap Assembly Master Mix (Takara). All DNA constructs were verified by sequencing (Genewiz and plasmidsaurus).
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the CPEC cloning assembly reaction was performed with the purified MotA-ASR insert and linearized vector using In-Fusion Snap Assembly master mix (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... siRNA Knockdown efficiency was tested as follows: 200 ng of RNA was reverse transcribed with Prime Script RT Master Mix (Takara) and quantified using QuantiFast SYBR Green RT-PCR Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... as per manufacturer’s protocol.The expression profile of target gene was evaluated using specific primers by using SYBR green RT-PCR master mix (Takara) in BioRad Real time PCR instrument ...
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bioRxiv - Immunology 2023Quote: ... The components of the master mix for the PCR and the PCR programs were set up according to the CloneAmp HIFI PCR protocol (Takara). All generated plasmids were sequenced before use (Eurofins Genomics).
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bioRxiv - Microbiology 2023Quote: ... The expression profile of target gene was evaluated using specific primers (Table-3) by using SYBR green RT-PCR master mix (Takara) in BioRad Real time PCR instrument ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was generated from each sample employing the Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc., Japan). Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc. ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR (qPCR) was performed on the CFX Connect Real-Time PCR Detection System using SYBR Green PCR Master Mix (TaKaRa). Primer pairs used were listed in Supplementary (Table S2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947) and following recommended manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and IV from M13cp following PCR amplification and ligation using the In-Fusion Snap Assembly Master Mix (Takara Bio, 638944). The resulting helper plasmids were transformed into DH5α competent cells (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was employed using the SYBR Green PCR Master Mix (Takara, Dalian, China) in a 48-well plate and analysed using a StepOne Real-Time PCR system (PE Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... The deletion of each Stx2a phage in the CmR transformants was confirmed by colony PCR using EmeraldAmp MAX PCR Master Mix (TaKaRa) and specific primers ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) is the final laboratory step of the scRT-qPCR workflow with the SYBR Green Master Mix (TaKaRa).
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bioRxiv - Molecular Biology 2020Quote: ... 2x Titanium Taq DNA Polymerase (Takara Bio.); 100 ng BSA per ul ...
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bioRxiv - Plant Biology 2020Quote: The polymerase chain reaction (PCR) was performed on genomic DNA using Emerald Amp MAX PCR Master Mix (Takara Bio, CA, USA) using the primers given in Table S2 ...
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bioRxiv - Evolutionary Biology 2021Quote: First amplified with primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) using EmeraldAmp GT PCR Master Mix (TAKARA BIO) to minimize the amplification of non-bacterial taxa under the following cycling conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
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bioRxiv - Biochemistry 2019Quote: ... RT was performed using Oligo(dT) random 6-mer primers from a Prime Script RT Master Mix kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... Gene specific primers listed in supplementary information were used in quantitative RT-PCR reactions containing SYBR green master mix (Takara #RR820). The Ct values were used to calculate Fold Change against spike-in control lncRNA (see results for details ...
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bioRxiv - Biochemistry 2019Quote: ... the URAT1/SLC22A12 gene promoter region was amplified using KOD One PCR Master Mix (TOYOBO) or PrimeSTAR® Max DNA Polymerase (Takara) with the following primers ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted using a Plant RNA Kit (R6827, Omega) and then reverse transcribed using PrimeScript™ RT Master Mix (RR036Q, Takara) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... was used to generate templates to synthesize first chain cDNA using the PrimeScript™ RT Master Mix kit (TAKARA, Dalian, China), with three biological replicates sample ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of total RNA was used for the cDNA synthesis reaction using PrimeScript™ RT Master Mix kit (TaKaRa RR036A) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR was performed as described (Pujol et al., 2008b) by using SYBR™ Green PCR Master Mix (TaKaRa). Values were normalized to those of act-1 and were analyzed by the cycling threshold method using the appropriate qRT-PCR primers (Supplementary Table S2) ...
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bioRxiv - Zoology 2020Quote: ... Diluted cDNA was amplified using gene-specific primers (Table 1) and the TB Green real-time PCR master mix (TaKaRa, Japan). RT–PCR was used to verify the accuracy of the RNA-Seq data and detect the mRNA expression of the MYL2 gene in tissues and C2C12 cells at least in triplicate with specific paired primers.
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed for gene expression using 2uL of 1:5 diluted cDNA with SYBR Green Realtime PCR Master Mix and Permix Ex Taq (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was reverse transcribed into 10 μL of complementary DNA (cDNA) with PrimeScriptTM RT Master Mix (Takara Biomedical Technology, China) at 37°C for 15 min ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
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bioRxiv - Cancer Biology 2022Quote: ... The expression of mRNA was examined by real-time quantitative-PCR (qRT-PCR) using SYBR Green PCR Master Mix (Takara, Japan) and a LightCycler480 Detection System (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... the full-length coding sequence of PLP with His6 at the N terminus was amplified by PCR and inserted into SalI-digested pBYR2HS vector using the In-Fusion Snap Assembly Master Mix (Takara Bio). The leaves of four-week-old N ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of the cDNA was used as DNA template in 15-µL amplification volumes with 400 nM of each primer and 7.5 µL of SYBR green master mix (Takara, Beijing, China) using the following cycling parameters ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned it (in frame) into the linearized LV-GFP plasmid using In-Fusion® Snap Assembly Master Mix (Takara #638947). High titer viruses were generated as previously described 56 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized from purified total RNA using PrimeScript RT Master Mix (Perfect Real Time; Takara Bio, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sample preparation for RT-qPCR from primary cultured neurons was performed using the RNeasy Mini Kit and PrimeScript RT Master Mix (Takara Bio). RT-qPCR was performed using KOD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from these retained samples was reverse-transcribed into cDNA using 250 ng as a template and the PrimeScriptTM RT Master mix (#RR036A, Takara, France). To provide a comparison point for the allele-specific expression in F1 larvae ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the synthesized cDNA was used to check the expression of the GFP transcript by semiquantitative PCR using GTPCR master mix (TAKARA BIO) for the GFP-specific primer ...
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bioRxiv - Genomics 2024Quote: ... The polymerase chain reaction (PCR) was performed on genomic DNA to amplify 16s rRNA using Emerald Amp MAX PCR Master Mix (Takara Bio) using the primers presented in Table S1.
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bioRxiv - Microbiology 2024Quote: ... and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was conducted with TaKaRa LA Taq® with GC buffer (TaKaRa) as follows ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
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bioRxiv - Systems Biology 2019Quote: ... The relative 2 standard curves real-time PCR was carried out on the cDNA samples using TaqMan master mix (Takara, Otsu, Japan) and a Q-6000 machine (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... the cDNA samples were synthesized from 500 to 1000 ng total RNA using the PrimeScriptTM RT Master Mix cDNA (Takara, Kyoto, JAP) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The levels of L6 transcripts observed in semi-Q PCR were further verified through qRT-PCR using SYBR master mix (Takara Bio, USA). Rice act1 was used as a house-keeping gene and the cDNA synthesized from NC was used to normalize the expression pattern by ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Developmental Biology 2021Quote: ... which then subjected to PCR using Taq Master Mix (Vazyme, p112-01) for RT-PCR or TB Green Premix EX Taq Ⅱ (Takara, RR820A) for quanti tative PCR ...