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Citations for Takara Bio :
351 - 400 of 1295 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and first-strand cDNA was synthesized using PrimeScript RT Master Mix (Perfect Real Time) (RR036Q; TaKaRa, Ohtsu, Japan) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Reverse transcription (RT)-PCR was performed using the Emerald Amp MAX PCR master mix (Takara Bio, Shiga, Japan). Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed on Thermo Piko Real 96 (Thermo) using SYBR Green PCR Master Mix (Takara #RR820A). The mRNA expression level was calculated by the 2-ΔΔCt method and the results were plotted by using tubulin as the reference gene ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNAs was synthesized from total RNA using the PrimeScript™ RT Master Mix (Perfect Real Time, Takara). Quantitative real-time PCR was performed in triplicate using PowerUp SYBR Green (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKaRa Bio), nuclease-free PCR-grade water ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were subcloned into pGEMHE (22) using the In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) with gene-specific primers and 15-bp sequences complementary to the ends of the linearized pGEMHE (Table S4 ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification was performed using Advantage GC 2 PCR kit (Clontech) and PCR products were cloned and sequenced.
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bioRxiv - Neuroscience 2023Quote: ... and the SYBR Premix Ex Taq GC (Takara Bio, Japan). Primers for qPCR (Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara Bio Europe, France). Quantitative real-time PCR was performed at 60°C using Takyon ROX SYBR MasterMix (Eurogentec ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed into cDNA using Takara PrimeScript RT master mix (RR036A; Takara Biotechnology Co., Ltd., Dalian China). RT-qPCR was performed using an ABI StepOne Plus Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA (1 μg) was applied to generate cDNA by means of PrimeScript™RT Master Mix (Takara, RR036A). The real-time quantitative PCR was performed by using SYBR Green Master Mix (Takara ...
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bioRxiv - Biophysics 2021Quote: ... using KOD One PCR Master Mix (TOYOBO) was subcloned into the AgeI- and NotI-digested EGFP-N1 vector (Clontech), and subsequently full-length EGFR fragment was subcloned into the NheI- and HindIII-digested the LgBiT-inserted EGFP-N1 vector ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA was assayed with ReverTra Ace qPCR Master Mix (TOYOBO, FSQ-201) in a Thermal Cycler Dice (TaKaRa) with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 μg of RNA using PrimeScriptTM RT Master Mix (catalog no. RR036A, Takara). qPCR was performed using TB Green Premix Ex TaqTM (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA synthesis from at least three biological replicates was performed using the PrimeScript™ RT Master Mix (TaKaRa, #RR036A) and real-time PCR was performed using SYBR Green I Master (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of the reaction mixture was prepared with 5 μl of SapphireAmp Fast PCR Master Mix (Takara #RR350B), 1 μl of each of forward and reverse primers (final concentration 0.2 μM) ...
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bioRxiv - Microbiology 2022Quote: ... Then 50 nl of primer pair solution mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were loaded onto the SmartChip ...
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bioRxiv - Microbiology 2022Quote: ... 50 nl each of the DNA samples mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were first loaded onto the SmartChip MyDesign Chip ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR was used to determine gene expression using the SYBR Green Realtime Master Mix (Takara, DaLian, China). Table 1 contains a list of all primers used in this study ...
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bioRxiv - Plant Biology 2023Quote: ... T-DNA insertions were then analysed using specific primers (Table S1) in PCR reactions with Emerald Master Mix (Takara). PCR conditions were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of total RNAs from each sample were reversely transcribed with PrimeScript RT Master Mix (TaKaRa, RR036A). Diluted cDNAs were mixed with primers and SsoAdvanced Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 300 ng was used as template for the reverse transcription using the PrimeScriptTM RT Master mix (#RR036A, Takara, France). The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272 ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... and then reverse-transcribed to cDNA using the PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of each RNA sample was reverse-transcribed with PrimeScript RT Master Mix (Perfect Real Time) (Takara Bio). qPCR was performed using the QuantiTect SYBR Green PCR Kit (QIAGEN) ...
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bioRxiv - Bioengineering 2024Quote: Primers oAS344 and oAS345 were then both used to amplify the cDNA using CloneAmp PCR Master Mix (Takara 639298) according to manufacturing protocol ...
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bioRxiv - Plant Biology 2023Quote: ... PrimeSTAR Max Premix (2x) (TaKaRa, Japan) was used for PCR with the degenerate primers listed in Supplemental Table S10 ...
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bioRxiv - Genomics 2022Quote: ... We used LA Taq with GC buffers (Cat# RR02AG, Takara Bio) or Multiplex PCR Plus Kit with Q-solution (Cat# 206152 ...
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bioRxiv - Plant Biology 2021Quote: ... with the SYBR Green Premix EX Taq GC™ (Takara, Japan) according to the manufacture’s instruction with the primers shown in Supplementary Table S2-2 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from cells or tissues with TRIzol Reagent (Life) and then used for reverse transcription by PrimeScriptTM RT Master Mix (TaKaRa). Synthesized cDNA was used for qPCR by 2× ChamQTM Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was pre-amplified by adding 2 uL of cDNA from each sample to 8 uL of preamp master mix [5 uL TaKaRa premix Taq polymerase (Clontech), 2.5 uL 0.2X Taqman pooled probe ...
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bioRxiv - Molecular Biology 2021Quote: ... First-strand cDNA was synthesized using about 1µg of total RNA and RT Master Mix with gDNA Remover (Takara Bio), which contains M-MLV reverse transcriptase ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of which was subject to PCR with primer pair 5′- AATGATACGGCGACCACCGAGATCT-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ and 5′-CAAGCAGAAGACGGCATACGAGAT-CTGATC-TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-GCTGCGCTCGATGCAAAATA-3′ using PrimeSTAR Max PCR master mix (R045A, Takara) to add sequencing adapter sequences to CASB barcode.
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bioRxiv - Immunology 2021Quote: ... and equal amounts of RNA for each sample were then reversed-transcribed using the PrimeScript™ RT Master Mix (Takara), following the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR amplification was run in an ABI 7500 HT (Life technology) with SYBR Green I Master Mix (TaKaRa), and the reaction mixture and program was carried out as described in our previous work (Wu et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR was carried out in triplicate with primers by using SYBR Green Realtime PCR Master Mix-Plus (Toyobo) in the Thermal Cycler Dice Real-Time PCR system (TaKaRa). PCR primers are listed in Supplemental Tables S2 and S3.
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV3 and harvested at DIV10 for qRT-PCR using SYBR green qPCR master mix (Takara). Total RNA was extracted from rat cortical neurons using the TRIzol reagent (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV4 and harvested at DIV11 for qRT-PCR using SYBR green qPCR master mix (TaKaRa). Total RNA was extracted from mouse cortical neurons using TRIzol reagent (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (same as used for generating the sequencing libraries) were reverse transcribed to cDNA using PrimeScript RT Master Mix (TaKaRa). cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Semi-quantitative PCRs (sqPCRs) were performed on 3 μl of cDNA template using Emerald Amp Max PCR Master Mix (Takara). The sequences of the primers used for PCR amplifications are included in Supplemental Table 4.
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bioRxiv - Developmental Biology 2022Quote: ... 1µl diluted cDNA was used as the template for each reaction with Emerald Amp GT PCR Master Mix (Takara, RR310). After appropriate amplification cycles ...
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bioRxiv - Bioengineering 2022Quote: ... were reverse transcribed into cDNA in a volume of 10 µl using the PrimeScript RT Master Mix (Perfect Real Time, TaKaRa) (37°C ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated as described in the RNA-seq section and 500 ng was reverse transcribed using the PrimeScript RT Master Mix (Takara). A volume of cDNA corresponding to 5 ng of the input RNA was subjected to quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... magnanima was assessed by PCR amplifying a cifB-like gene (Extended Data Table 3) in the WOwHm-t76 region with the Emerald Amp Max Master mix (TaKaRa) at 94°C for 3 min ...
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bioRxiv - Genomics 2019Quote: ... PCR amplifications were performed on 1 μl of 1:20 dilution of the cDNA template in a final volume of 50 μl using the EmeraldAmp PCR Master Mix (Clontech). Appropriate annealing temperatures and cycle conditions were adjusted to individual primer pairs (see supplemental methods).
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was reverse transcribed to complementary DNA (cDNA) using a Prime Script™ RT Master Mix Kit (TaKaRa, China) and the following procedure ...
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bioRxiv - Cell Biology 2022Quote: ... The purified fragments were integrated into digested vectors by using In-fusion Snap Assembly Master mix (Takara Bio, Japan, 638947). Our lab stock plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP) gene using the In-Fusion Snap Assembly Master Mix (TaKaRa). Forty-eight hours after transfection ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from retinas with TRIzol Reagent (Life) and then used for reverse transcription by PrimeScript RT Master Mix (TaKaRa). Synthesized cDNA was performed with 2× ChamQ Universal SYBR qPCR Master Mix (Vazyme ...