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Citations for Takara Bio :
451 - 500 of 1295 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was perfor med using a qPCRsoft (Analytik Jena AG, Germany) with a SYBR®Green PC R Master Mix Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR mix was prepared using the plasmids obtained from TA cloning and CloneAmp HiFi PCR Master Mix according to manufactures recommendations (In-Fusion® HD Cloning Plus, Takara, 638910). This is followed by in-fusion reaction for site directed mutagenesis as we have done previously (Kocabas and Aslan ...
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bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from organoids using Nucleo Spin (MACHEREY NAGEL) and cDNA was synthesized from 500 ng of total RNA using PrimeScript™ RT Master Mix (TAKARA BIO). We performed quantitative reverse-transcription PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of total RNA was used to produce cDNA using the TaKaRa PrimeScript RT master mix (TaKaRa Bio, Otsu, Japan). Two microliters of cDNA was then used for quantitative PCR ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKara Bio, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA libraries for each sample were constructed from 100 ng of RNA using the PrimeScript RT master mix (Takara Bio, Kutatsu, Japan) under manufacturer specifications ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then frozen and sent to Novogene for ultra-low-input bulk RNA sequencing or converted into cDNA with the PrimeScript RT Master Mix (Takara Bio, RR036A) for quantification of mRNA level using qPCR4 ...
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bioRxiv - Microbiology 2023Quote: Total RNA from intestinal tissue and organoids was extracted using TRIzol method according to the precise instructions and cDNA was synthesized using PrimeScript™ RT Master Mix (Takara, Japan). With cDNA as template ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... To amplify the canvas and activity-encoding regions on the recorder plasmid construct,0 5µL of culture sample was directly used in a 10µL PCR reaction using PrimeStar Max master mix (Takara Bio, San Jose, CA) under conditions recommended by the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed with GeneAce SYBR qPCR Mix α No ROX (Nippon Gene, Tokyo, Japan) in Thermal Cycler Dice Real Time System II (Takara). Each Actin1 genes (PtActin1 and TpActin1 ...
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bioRxiv - Plant Biology 2021Quote: ... Each PCR was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR amplification was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), gene specific primers (300 nM of each) ...
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bioRxiv - Cell Biology 2021Quote: ... YFP-FKBP and Tom20-CFP-FRB were inserted to the digested sites using In-Fusion Snap Assembly Master Mix (Takara Bio USA, 638947). To visualize Golgi ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (Takara Bio, Shiga, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Molecular Biology 2023Quote: ... with its native promotor and was subcloned into a modified pACYC vector using In-Fusion cloning strategy (In-Fusion® Snap Assembly Master Mix; TaKaRa Cat. # 638947). The DNA of P ...
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bioRxiv - Molecular Biology 2023Quote: ... The insert was cloned into the pAM_1C vector using the In-Fusion snap assembly master mix according to manufacturer’s instructions (Takara Bio, Kyoto, Shimogyō-ku, Japan). The purified plasmid was verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR assays after ChIP were performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO, Japan) and the Thermal Cycler Dice Real Time System II (TAKARA BIO, Japan) according to the manufacturer’s protocols with the primers below ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR polymerase capable of handling GC-rich amplicons was used (PrimeSTAR GXL Premix, Clontech). The resulting DNA amplicon ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Evolutionary Biology 2020Quote: PCR amplification was carried out using the Advantage GC 2 PCR Kit (Clontech Catalog #639119), and cloning was carried out using standard procedures (primer sequences are listed below).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Homology cloning was performed using In-Fusion® Snap Assembly Master Mix for homology cloning according to manufacturer protocols (TAKARA Bio Inc., San Jose, CA). All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... and WndC130S-KD::GFP were generated by site-directed mutagenesis using In-Fusion® Snap Assembly Master Mix (Takara Bio USA, Inc, San Jose, CA). UAS-Wnd-C130S::GFP and UAS-WndΔ101-200::GFP were generated by site-directed mutagenesis ...
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bioRxiv - Cell Biology 2021Quote: ... PrimeScript II (Takara) was used for the reverse transcription ...
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bioRxiv - Genomics 2022Quote: ... For PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), a 50 μl -reaction system was prepared as follows ...
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bioRxiv - Microbiology 2020Quote: ... Semi-quantitative PCR was conducted using LA taq with GC buffer I (Takara medical co. ltd) using the methods in manual.
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bioRxiv - Molecular Biology 2023Quote: ... or 100 ng of genomic DNA was amplified with Advantage GC 2 polymerase (Takara Bio 639114) using 1 M GC Melt and primers spanning the region from upstream of the splice donors to the 3’ end of the repeat ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out using TB Green® Premix Ex Taq™ GC (TaKaRa, RR071Q). Gene expression data were normalized to sigA and expressed as fold change using the 2−ΔΔCt method compared to wild-type strain or untreated control.
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bioRxiv - Genomics 2022Quote: ... 25 μl PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), 20 ng DNA and NF water to a total volume of 50 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Random Primer Mix (Takara) and 200 U SMARTScribe Reverse Transcriptase (Takara) ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR Green Mix (TaKaRa) was used for quantitative PCR ...
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bioRxiv - Microbiology 2023Quote: ... Mighty Mix (Takara Bio) was used for ligation ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II or TB Green Premix Ex Taq II (Takara Bio). The primer sequences used were shown in Table S5 (Tara et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... 3mM dNTP mix (Takara Bio)) ...
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bioRxiv - Cell Biology 2021Quote: ... Mighty Mix (Takara Bio, 6023) and transformed into One Shot TOP10 Chemically Competent E.coli (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-GGGRCCARKG GATAGACHGATGG-3’ (designed according to the C segment of mouse IgG antibody heavy chain sequence ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-5’ s-CTTCAGAGGA AGGGTGGAAACAGG-3’ (designed according to the C segment of mouse IgG antibody light chain sequence ...
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bioRxiv - Genomics 2022Quote: ... PCR Master Mixkit was purchased from TaKaRa Company ...
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bioRxiv - Microbiology 2021Quote: ... using FastStart Universal SYBR Green Master (Takara). The level of β-actin expression was used to normalize the data ...
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bioRxiv - Developmental Biology 2021Quote: DN-Brn2 expression vector was generated by PCR amplification of Brn2 DBD and addition of NES sequences using primers ATC ATC GAA TTC GAG AGT CAT GCT TCA ACT TCC TCC TCT TGA ACG CCT TAC CCT TGG AGG AGG AGG ACC GGG CCA CCC AGG CGC GCA C and GAT GAT GGA TCC CCA AGG GTA AGG CGT TCA AGA GGA GGA AGT TGA AGT CCT CCT CCT CCA CCC CCA TAC ACA TCC TCG GC and mBrn2 template plasmid (Sugitani et al. 2002) into mCherry N1 (Clontech). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara). PCR conditions were optimized for long fragments as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... SYBR Premix Ex Taq II (TaKaRa) was used to perform quantitative RT-PCR in Agilent Mx3005P ...
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bioRxiv - Microbiology 2021Quote: ... SYBR Premix Ex Taq II (Takara) was used for quantitative real time PCR for quantification of target gene expression ...
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bioRxiv - Microbiology 2019Quote: ... The pCold™ II (Takara Bio) and pET50b-Amp plasmids for protein expression in E ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with SYBR Premix ExTaq II (Takara). Pre-designed primer sets for mouse TNF-α (primer set MA097070) ...