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Citations for Takara Bio :
301 - 350 of 1295 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara). Quantitative real-time PCR was performed at 60°C using Takyon No ROX SYBR 2X MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Immunology 2023Quote: ... and then converted to cDNA using PrimeScript™ RT Master Mix (Perfect Real Time, Takara, RR036A). Quantitative real-time reverse transcription-polymerase chain reaction (qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ng of RNA was used for reverse transcription using PrimeScript RT Master Mix (Takara, USA). PCR reactions were performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Pathology 2024Quote: ... Real-time PCR was performed by using an SYBR Green PCR Master Mix (TaKaRa, Dalian, China) and the primers listed in Appendix B.
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bioRxiv - Microbiology 2021Quote: ... The Plus25neutral mutant was gener- ated via Gibson assembly using CloneAmpTM 2X HiFi PCR mix (Takara Bio USA, Mountain View, CA) for backbone amplification ...
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bioRxiv - Cell Biology 2022Quote: ... For gene analysis the cDNA was amplified using gene specific primers by qPCR with Takara 2X SYBR Green Mix (Takara RR420A) and analyzed in real time PCR machine (7500 Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR reactions were performed in triplicate with TaqMan Master Mix: Premix Ex Taq (TaKaRa, Otsu, Japan) in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Amplifications were typically performed in 20 μL reaction volumes containing 1× EmeraldAmp GT PCR Master Mix (Takara Bio Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with standard (DreamTaq - Thermo Fisher Scientific, or EmeraldAmp Max HS PCR Master Mix - TaKaRa Bio Inc) DNA polymerases ...
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bioRxiv - Pathology 2019Quote: ... An aliquot of the cDNA was mixed with 25 µL SYBR® Green PCR Master Mix (TaKaRa) and 10 pmol of each specific forward and reverse primer ...
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bioRxiv - Cancer Biology 2019Quote: ... then 500 ng of total RNA was reversely transcribed into cDNA using PrimeScriptTM RT Master Mix (TaKaRa) according to the manufacturer’s instructions in a total volume of 10 μL ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™, Takara) and a CFX96 thermal cycler (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs from 1 μg DNase-treated purified RNA were obtained using PrimeScriptTM RT Master Mix (#RR036A, TAKARA) following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... synthesized cDNA with the ReverTra Ace qPCR RT Master Mix (TOYOBO) and used TaKaRa Ex Taq (TaKaRa) for amplification of NSP and MA genes ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA (cDNA) was prepared using the 5X Prime Script RT Master Mix (Takara RR-036A-1) with 1 μg of total RNA ...
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bioRxiv - Plant Biology 2021Quote: ... The first strand of cDNA was synthesized using PrimeScript™RT Master Mix (Perfect Real Time) (TaKaRa). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: The cDNA synthesis kits and SYBR Green Master Mix were obtained from Clontech (Mountain View, CA, USA). Dexamethasone and Granzyme B inhibitor Z-AAD-CMKwere purchased from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR assays were performed on a qTOWER apparatus (Analytic Jena) using SYBR Green master mix (TaKaRa). Gene expression was normalized for the expression of 36B4 ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and cDNAs were prepared using PrimeScriptTM RT Master Mix kit (Takara, RR036A). Analysis of mRNA expression was performed with SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The 20-µL reaction volume comprised 10 µL 2× SYBR Green PCR Master Mix (Takara, Dalian, China), 0.2 µM each primer ...
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Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... qPCR reactions were run with SYBR® Premix Ex Taq (Tli RNAse H Plus) master mix (Clontech) on LightCycler® 96 Instrument and analysed using LightCycler® 96 Application (Roche Life Science) ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription with 1 μg of total RNA was conducted using PrimeScript™ RT Master Mix (Takara) following the manual ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA by Prime script RT master Mix (Takara, RR036A). RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (400 ng) was then reverse-transcribed using PrimeScript RT Master Mix (#RR036A; TaKaRa, Kusatsu, Japan). The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus ...
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bioRxiv - Physiology 2023Quote: ... The qRT-PCR was conducted in a 20μl reaction volume employing SYBER Green Master Mix (TaKaRa, Japan). The qRT-PCR was carried out using the Applied Biosystems QuantStudio3 real-time PCR machine (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... we used the following primer sets and In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) per the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by SYBR Green PCR Master Mix (Takara). The primer sequences for qRT-PCR were listed in Table S15.
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bioRxiv - Cancer Biology 2024Quote: ... The isolated RNA was reverse transcribed into cDNA using the PrimeScript™ RT Master Mix (Takara: RR036B) according to the the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... The linear PCR product was then re-circularized with In-Fusion Cloning Master Mix (Takara Bio, 638948) according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
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bioRxiv - Microbiology 2020Quote: ... or Premix ExTaq 2x (Takara) reagents and the StepOne Plus (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed using Takara PrimeScript™ RT Master Mix (Clontech Laboratories, USA). qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: The mRNA and miRNA cDNA libraries are reverse-transcribed by PrimeScript™ RT Master Mix (TaKaRa, Dalian, China) and miRNA 1st Strand cDNA Synthesis Kit (by stem-loop ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized by reverse transcription of 1 μg RNA using PrimeScrip RT Master Mix(Takara). Quantitative real time-PCR was performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg of total RNA was subsequently reverse transcribed to cDNA using PrimeScript RT Master Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse transcription was performed to generate cDNA using the PrimeScript RT Master Mix (RR036; Takara, Kusatsu, Japan). The RNase-Free DNase Set (79254 ...
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bioRxiv - Pathology 2022Quote: ... Single-stranded cDNA was reverse transcribed using Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc.), and used for subsequent real-time PCR reactions ...
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bioRxiv - Plant Biology 2021Quote: ... Synthesis of cDNA and quantitative PCR was conducted using the PrimeScript RT Master Mix (Takara Bio, Kusatsu, Japan) with Thermal Cycle Dice TP800 (Takara Bio) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of RNA was reverse transcribed in a 20 μL volume with RT PCR master mix (TaKaRa) as per the manual instruction ...
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bioRxiv - Neuroscience 2020Quote: ... each cDNA was subjected to PCR genotyping using Emerald AMP HS PCR Master Mix (Takara Bio USA #RR330B) and primers shown in Supplementary Table S5 ...
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bioRxiv - Microbiology 2021Quote: ... and target genes were amplified by conventional PCR (50 µL reactions) with EmeraldAmp GT PCR Master Mix (Takara Bio ...
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bioRxiv - Immunology 2020Quote: ... The reverse transcription of RNA into cDNA was performed using a Prime Script TM RT Master Mix (Takara). The following was the reaction system ...
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bioRxiv - Plant Biology 2020Quote: ... Partial genomic sequences of CpPT1 were PCR amplified in these preparations using SapphireAmp Fast PCR Master Mix (Takara) and the primer pair citron_Fw and citron_Rv (Supplementary Table 8) ...
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bioRxiv - Plant Biology 2021Quote: ... The transcript abundance was measured in 10 µL volume of the SYBR Green PCR Master Mix (Takara, Japan) containing cDNA corresponding to 5 ng of total RNA with gene-specific primers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The 2-μg RNA sample was reverse transcribed using the PrimeScript™ RT Master Mix (Takara, Shiga, Japan). Quantitative real-time PCR (qPCR ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng of total RNA was used in 10 μL PrimeScript™ RT Master Mix (TaKaRa, Tokyo, Japan) as described in the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 500 ng of total RNA was reverse transcribed into cDNA using PrimeScript™ RT Master Mix (Takara, RR036A). qPCR analyses were performed using SYBR Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2022Quote: ... The genes were then cloned into linearized pCMVR backbone using In-Fusion HD Cloning Kit Master Mix (Clontech). Antibodies were produced in Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was conducted on Bio-Rad CFX96 using the SYBR green PCR master mix (TaKaRa, Japan) with the primers listed in Appendix 1—key resources table ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 400 ng of RNA was used to prepare cDNA using Primescript RT Master Mix (Clontech) at 42 °C following manufacturer’s instructions ...