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Citations for Takara Bio :
4451 - 4500 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... the total RNA of three samples in each group was extracted with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), and linear RNA was then removed with Rnase R (GENESEED ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... of MMP9 or HSF2 were inserted into the linear vector upstream of the luciferase reporter gene using the In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-7 and ErbB2-overexpressing MCF-7 cells were routinely tested using the CycleavePCR Mycoplasma Detection Kit (Takara bio, CY-232). None of the cell lines were contaminated with mycoplasma.
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for the preparation of libraries using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the amplicons were then ligated into a pUAST-attB vector digested by EcoRI/NotI using In-Fusion HD Cloning Kit (Takara, 639649). The landing site for each construct was {3xP3-RFP.attP}ZH-86Fb ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) following the instructions of PrimeScript RT reagent kit (Takara, Beijing, China). qRT-PCR was performed using the AceQ Universal SYBR qPCR Master Mix (Vazyme) ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng supercoiled plasmid pBR322 obtained from the FastPure Plasmid Mini Kit (Vazyme, DC201-01) or relaxed plasmid pBR322 (derived from supercoiled pBR322 digested by Topoisomerase I, Takara, 2240A), along with 6 μl 5× buffer (170 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... Library preparation of RNA samples was performed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara 634485). Two independent pooled libraries (samples from mice with spontaneous disease and samples from mice with pristane-induced disease ...
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bioRxiv - Immunology 2024Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara, 634888) and 200 pg of cDNA was used to generate sequencing libraries with the NexteraXT kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual genomic and plasmidic DNA was removed using DNA-free kit (InvitrogenTM) and 500 ng of RNA were retro-transcribed with PrimeScript RT Master Mix (Takara Bio) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning reactions were performed at 50 °C for 1 h using the In-Fusion® HD Cloning Kit (Takara Bio). The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Resulting amplicons were mixed with the digested backbone and joined in a single reaction by In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... plasmid and cloned within exon 4 (between base 43-44) and exon 5 (between base 71-72) of the circ-HDGFRP3 sequence by In-Fusion Cloning Kit (Takara Bio) to obtain the final constructs p-circ-Ex4 and p-circ-Ex5.
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bioRxiv - Biochemistry 2024Quote: ... We introduced a T44C mutation and 108C mutation into the GB1-loop by site-directed mutagenesis using a PrimeSTAR mutagenesis basal kit (Takara Bio).
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bioRxiv - Cell Biology 2024Quote: ... GS linker human IgG1 FC region from pcDNA3-sACE2v2-Fc (IgG1) and PGK promoter puromycin resistance region cloned into lenti CAG-FLAG-dCas9-VPR using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Pathology 2024Quote: Expression for IFN-related genes was conducted by reverse transcription using the PrimeScript RT Reagent Kit (Perfect RealTime, Takara Bio Inc.). qPCR reaction was performed in duplicate using Takyon ROX SYBR MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Genomics 2024Quote: ... 152 ∗106 GPRTG cells were seeded into a 2-STACK culture chamber (Cornig) and transfected 24 h later with 238 µg transfer plasmid using the CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Then the RNA-seq library was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit (TaKaRa Bio, USA). This was performed in Shpokayte et ...
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bioRxiv - Synthetic Biology 2024Quote: ... the rRNA-depleted and fragmented total RNA fraction was converted to sequencing libraries using the SMARTer® smRNA-Seq Kit for Illumina (Catalog number #635030) from Takara Bio USA ...
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bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Immunology 2024Quote: ... The damaged lung areas of the infected mice and the corresponding lung areas of the uninfected control mice were excised to isolate total RNAs using TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, 9767), which were reverse transcribed into cDNAs using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments were joined through SOE-PCR and subsequently ligated into the pOPINK vector using the In-Fusion HD Cloning Kit (Takara Clonetech). A pOPINE vector containing C-terminally Flag-tagged human USP30 (1-517 ...
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bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
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bioRxiv - Systems Biology 2024Quote: ... and pooled lentiviral stocks harvested at 48 hr and 72 hr post-transfection were concentrated 20x with the Lenti-X Concentrator kit (Takara #631232). K562 cells (ATCC ...
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bioRxiv - Microbiology 2024Quote: ... Smc6 cDNA was amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (TaKaRa, Cat#R026B) using specific primers (Supplementary Table 3) ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the viral stock was determined using p24 ELISA (Lenti-X™ p24 Rapid Titer Kit #631476 from Takara). And the doses used in all experiments were chosen by infecting primary CD4 T cells to reach appropriate levels of infection.
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from leaves of 3-week-old Arabidopsis plants or 40-day-old rice plants using the Minibest plant RNA extraction kit (Takara, 9769) and three independent biological replicates were performed ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from leaves of 3-week-old Arabidopsis plants or 1-week-old rice seedlings with Minibest plant RNA extraction kit (Takara, 9769) and three independent biological replicates were performed ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration was assessed by Nanodrop and RNA-seq libraries were prepared from 10 ng of total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... by following manufacturer’s instruction and was reverse-transcribed into first-strand cDNA using PrimeScript™ 1st strand cDNA Synthesis Kit (Takara Bio) in accordance with manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysis was quantified by measuring the lactate dehydrogenase (LDH) amount in the cell supernatant using the LDH cytotoxicity kit (Takara, Clontech) according to the manufacturer’s instructions and expressed as a percentage of total LDH release ...
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bioRxiv - Immunology 2024Quote: ... Cells were then cloned by limiting dilution and individual clones were screened for V203A mutation by the Guide-it Knock-in Screening Kit (Takara Bio). Homozygous clones were sequenced by Sanger sequencing to confirm the presence of a homozygous mutation without any off-target effects.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA templates were generated by reverse transcription using a double-primed RNA to cDNA EcoDry™ Premix kit (TaKaRa, Clontech, https://www.takarabio.com/about/our-brands/clontech) ...
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA templates were generated by reverse transcription using a double-primed RNA to cDNA EcoDry™ Premix kit (TaKaRa, Clontech ...
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bioRxiv - Microbiology 2024Quote: ... The PPI4 backbone was digested with NotI and PstI and In-Fusion cloning was carried out using In-Fusion Snap Assembly kit (Takara Bio) according to the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... PCR experiments were performed in a total volume of 10 μl by using an SYBR Premix Ex Taq kit (Takara Bio). The reaction mixtures were incubated for 2 min at 95℃ ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was used to synthesize cDNA using the SMART-Seq v4 full-length transcriptome analysis kit from Takara (product # 634888) using protocol B specified in the manual on page 12 ...
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bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc, USA). After shearing of cDNA by Covaris ultrasound water bath sonicator and purification by Ampure beads (Beckman Coulter ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Neuroscience 2024Quote: ... The 3×FLAG-tagged hnRNPA1 cDNA was subsequently amplified from the p3xFLAG vector by PCR and cloned with the use of an In-Fusion Cloning Kit (Takara Bio) into the pEN_TTGmiRc2 vector (Addgene) ...
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bioRxiv - Neuroscience 2024Quote: ... The pGL4.12-UNC13A-ΔR vector for the deletion mutant lacking an intact REST binding site was constructed with a PrimeSTAR Mutagenesis Basal Kit (Takara Bio). Luciferase activities of cell lysates were measured with a Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... coli genomic DNA extracted from BW25113 wild-type strain and inserted into the pUC18 vector with the seamless cloning method (In-Fusion HD Cloning Kit, TaKaRa Bio). For the expression of recombinant RNase I wild type and mutants ...
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bioRxiv - Molecular Biology 2024Quote: ... using 50 ng of captured RNA as input for the Takara SMARTer Stranded Total RNA-Seq Kit v3-Pico Input Mammalian (Takara 634451), and sequenced using the NOVA-Seq platform ...