Labshake search
Citations for Takara Bio :
401 - 450 of 747 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... VSV-G-pseudotyped retrovirus was produced in GP2–293 packaging cells (Clontech) and VSV-G-pseudotyped lentivirus ...
-
bioRxiv - Developmental Biology 2021Quote: ... The resulting plasmids were co-transformed into Saccharomyces cerevisiae strain AH109 according to the protocols in the Matchmaker GAL4 Two-Hybrid System 3 (Clontech). The yeast cells were cultured on SD/-Leu-Trp (-LW ...
-
bioRxiv - Genomics 2021Quote: The NucBlue and DAPI co-stained single-nuclei suspension (60□cells/µl) was distributed to eight wells of a 384-well source plate (Takara) and then dispensed into a barcoded SMARTer ICELL8 3’ DE Chip (Takara ...
-
bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
-
bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
-
bioRxiv - Pathology 2022Quote: ... The bait and prey vectors were co-transformed into yeast strain Y2H Gold according to the Yeast Protocols Handbook (Clontech), diluted on SD/-Leu-Trp/AbA for selection of positive colonies ...
-
bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
-
bioRxiv - Microbiology 2022Quote: ... The purified ds cDNA and SmaI linearized pGADT7-Rec were co-transformed into Y187 yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on to the SD/-Leu agar plates and incubated at 30°C for 3–4 days ...
-
bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
-
bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitated (RGA-GFP and RGAm2-GFP) and co-immunoprecipitated (IDD2-RFP) proteins were detected by western-blot with anti-GFP (JL8; Clontech) and anti- RFP (6G6 ...
-
bioRxiv - Genetics 2020Quote: ... The target vector and pEF1a-pac vector were co-transfected (5:1 ratio) to E14 mESCs using Xfect according to the manufacture’s instruction (TaKaRa, Inc). 48 hours after transfection ...
-
bioRxiv - Developmental Biology 2021Quote: ... the distal region of blastemas was co-injected and electroporated with a reporter plasmid (pEGFP-N2 or pRFP-N2; Clontech) together with the Tig1 ...
-
bioRxiv - Plant Biology 2020Quote: ... bait plasmids and prey plasmids were co-transformed into yeast strain AH109 according to the manufacturer’s protocol of GAL4-based yeast two-hybrid system (Clontech, USA). Then ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
-
bioRxiv - Molecular Biology 2023Quote: ... and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). Transfected cells were cultured in selective media with 100 μg/ml of hygromycin B for 2 weeks ...
-
bioRxiv - Biochemistry 2023Quote: ... Constructs used to test protein-protein interactions were co-transformed into the yeast Saccharomyces cerevisiae strain AH109 using the Matchmaker Gold yeast two-hybrid system (Clontech). Empty pDEST-GBKT7 and pDEST-GADKT7 vectors were used as negative control ...
-
bioRxiv - Immunology 2022Quote: ... HEK 293T cells were seeded in a 6-well plate at a density of 6 × 105 cells/well and co-transfected with pSIN-siU6 – shSLFN11/ shSLFN12/ shSc (2 μg) along with plasmids pGP (1 μg, Takara) and pPE ampho (1 μg ...
-
bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
-
bioRxiv - Molecular Biology 2024Quote: ... The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340), overexpressed in E ...
-
bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
-
bioRxiv - Plant Biology 2023Quote: ... Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech), followed by a selection of transformants on 2% SD (-Leu/-Trp ...
-
bioRxiv - Microbiology 2022Quote: ... HeLa wild-type or HeLa-HSF1i cell monolayers were mock-transfected or co-transfected with HCoV genomic RNA (1 μg/ml) and pCMV-GFP vector (Clontech) using TransIT-mRNA Transfection Kit (Mirus Bio ...
-
bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
-
bioRxiv - Developmental Biology 2024Quote: ... MS2-orf147 hybrid RNA expressing in pPRP1 vector and identified PPRs expressing in prey vector (pGADT7) All three components were then co-transformed into yeast host (Saccharomyces cerevisiae strain Y187 (Takara) at equimolar concentrations in DDO/X/A [SD/–Leu/–Trp/X-a-Gal/AbA] media (containing kanamycin to prevent bacterial contamination ...
-
bioRxiv - Cell Biology 2024Quote: ... The ERH expression vector with C-terminally tagged mTurquoise2 used in co-IP-MS/MS was cloned in the pEGFP-C1 backbone (Clontech).
-
bioRxiv - Genomics 2020Quote: ... and DNA contaminants were removed with RNase-free DNase I (TaKaRa, Beijing, China); the purified RNA quantity and quality were checked using Nanodrop 2000 spectrophotometer (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50) for Split-QF2 lines and eluted in ultrapure water prior to injection ...
-
bioRxiv - Cell Biology 2021Quote: ... and pH7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The cells in the suspended solution were sonicated using the Q125 sonicator (Qsonica ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was first treated by 1 Unit DNase I (RNase free, TaKaRa, China) at 37.0 °C for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... Extracted RNA (20µg) was treated with recombinant DNase I (RNAse free) (Takara, Japan) and then cleaned-up using RNeasy spin column (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: RNA extract used for sequencing above was treated with RNase-Free DNase (Takara) to remove contaminated DNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe) under standard conditions ...
-
bioRxiv - Plant Biology 2021Quote: ... and c-DNA was prepared using 2 µg total RNA samples (Takara Bio, UK). The c-DNA samples were diluted ten times and an aliquot of 2 µl of each sample per reaction was used for qRT-PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µg of RNA per sample were incubated with 5U MNase (Takara Bio, 2910A) supplemented with 5 mM CaCl2 in 200 µl final volume ...
-
bioRxiv - Developmental Biology 2024Quote: ... For beta-catenin overexpression the CHIR99021 was substituted with 1 µg/ml doxycycline (Clontech).
-
bioRxiv - Cancer Biology 2022Quote: ... 2 µg of plasmids were diluted in 200 µL Xfect transfection reagent (631317, Takara). The mixture was incubated at room temperature for 15 min ...
-
bioRxiv - Neuroscience 2024Quote: ... 1.5 µg RNA was added to cDNA conversion (Clontech RNA to cDNA Random Hexamers) tubes and placed in a thermal cycler and heated at 42⁰C for 75 minutes and at 80⁰C for 15 minutes ...
-
bioRxiv - Bioengineering 2021Quote: Plasmids were constructed by standard molecular biology methods such as polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific amino acids were generated by overlap-extension PCR ...
-
bioRxiv - Bioengineering 2024Quote: ... All the other plasmids used in this study are cloned by following the standard protocol of Infusion HD (Takara).
-
bioRxiv - Neuroscience 2024Quote: ... Site-directed mutations were introduced into KCNQ2 and KCNQ3 by standard PCR techniques using PrimeSTAR Max DNA Polymerase (Takara). For whole-cell patch clamp recording of KCNQ3 channels in HEK293T cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
-
bioRxiv - Plant Biology 2020Quote: ... We generated a plasmid encoding Pikh-HMA in pGBKT7 and co-transformed it into chemically competent Y2HGold cells (Takara Bio, USA) with the individual AVR-Pik variants in pGADT7 as described previously [20 ...
-
bioRxiv - Systems Biology 2022Quote: ... Plasmids containing mKO2-hCdt1 (30-120) and mAG-hGeminin (1-110) were co-transfected with envelope and packaging plasmids into LentiX-293T cells (Takara bio) to generate lentiviral particles ...
-
bioRxiv - Microbiology 2024Quote: ... After co-cultivation, the organoids were collected and resuspended in 4% paraformaldehyde (PFA, Servicebio, China) at 4 [or RNAiso Plus (Takara, Japan) at −80□ for further analysis ...
-
bioRxiv - Developmental Biology 2020Quote: All cloning and mutagenesis was performed using In-Fusion® restriction-free cloning (Takara). Linear PCR products and/or vectors (as detailed below ...
-
bioRxiv - Molecular Biology 2020Quote: ... and DNA templates were digested by RNase-free DNase I (Takara Bio, Shiga, Japan).
-
bioRxiv - Cell Biology 2020Quote: ... nuclease-free water and Titanium Taq DNA Polymerase (Clontech Laboratories Inc.; Takara Bio Group). Telomerase activity standard curves were generated using four 10-fold serial dilutions (0.02 ...
-
bioRxiv - Cell Biology 2020Quote: ... nuclease-free water and Titanium Taq DNA Polymerase (Clontech Laboratories Inc.; Takara Bio Group). Telomerase activity standard curves were generated using four 10-fold serial dilutions (0.02 ...
-
bioRxiv - Cell Biology 2023Quote: ... Greenberg) were cultured in DMEM supplemented with 10% tetracycline-free fetal bovine serum (Takara) and antibiotics ...
-
bioRxiv - Immunology 2024Quote: ... and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio). For immunocytokines ...