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Citations for Takara Bio :
501 - 550 of 747 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... with 5 µg of pTetOne NTF2 (pDL66) and 100 ng of linear hygromycin marker (#631625, Clontech). After 4 hours at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... the expression of GADD34Δ and K3L was induced by adding 1 µg/ml of doxycycline (Takara). After one additional day ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µg RNA was reverse transcribed into cDNA using a cDNA synthesis kit (Takara Bio) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed to cDNA using SMART MMLV reverse transcriptase (Takara) and Oligo(dT)20 as a primer ...
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bioRxiv - Pathology 2024Quote: ... cDNA was synthesized from 1 µg of RNA with a PrimeScript RT reagent Kit (Takara, RR037A). Pluripotency genes were amplified with gene-specific primers by PCR using Taq DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using the Primescript RT Master Mix (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Transgene expression was induced by treating the cells with 1.5 µg/mL doxycycline (Clontech, cat# 631311) for 24 hours.
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bioRxiv - Bioengineering 2024Quote: ... MSCs and chondrocytes were fluorescently labeled using mitochondrial or cytoplasm targeted lentiviruses driven by an EF1α promoter for co-cultures (Takara Bio USA, Inc.). Mitochondria were labeled with GFP or mCherry (0017VCT ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were generated in HEK 293T cells by co-transfection of the lentiviral vector pLVX-luc-IRES-puro (Clontech, Mountain View, CA) with the pCMV-dR8.9 and pCMV-VSV-G plasmids (both obtained from Addgene ...
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bioRxiv - Neuroscience 2024Quote: Library preparation and RNA sequencing were performed by Novogene Co using the SMART-Seq v4 PLUS Kit (Takara Bio, Kusatsu, Shiga, Japan, # R400752). Total RNA was reverse transcribed into the first-strand cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Two micrograms of RNA from each sample was used to synthesize cDNA using PrimeScript RT reagent kit (Takara Biotechnology Co. Ltd. Dalin, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Immunology 2022Quote: ... cells were transfected with pCMV-VSV-G (1.5 μg) and pRETROX TET3G (2.5 μg) (Clontech) using Lipofectamine 2000 (10 μL) ...
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bioRxiv - Immunology 2024Quote: ... and pMD2-VSV-G to HEK293T cells using the CalPhos Mammalian Transfection Kit (Clontech Laboratories). Viral supernatants were filtered through 0.45 μm low-protein-binding filters ...
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bioRxiv - Microbiology 2022Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: ... and DNA contamination was removed by treating it with RNase-free DNase I (Takara Bio, China). Approximately 1 μg of total RNA was used for the first-strand cDNA synthesis using RevertAid cDNA synthesis kit (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The trace amount of DNA was removed through incubation with RNase-free DNase I (TaKaRa, Japan) at 28 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 3.5 μL nuclease-free water was added to 4 μL of 5x PrimeScript buffer (Takara, USA), 1 μL RNAse OUT (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA was amplified by polymerase chain reaction (PCR) using KOD PLUS DNA polymerase (TOYOBO Co., Ltd., Osaka, Japan) or TaKaRa Ex Taq (Takara Bio Inc., Otsu, Japan). Restriction endonucleases ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with the appropriate chemicals at the following concentrations: 1 µg/ml doxycycline (Clontech, 631311), 2.5 µM MG132 (APExBIO ...
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bioRxiv - Cell Biology 2024Quote: The transfected cells were selected in the medium containing appropriate selection drugs (0.5 µg/mL puromycin (TAKARA) for the selection of PuroR expression ...
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bioRxiv - Immunology 2024Quote: ... non-tissue cultured treated 24-well plates were pre-coated overnight with 50 µg/mL Retronectin (Takara) and washed once with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Six-well plates were coated with retronectin (50 µg/2 mL/well, (Takara Bio Inc., Kusatsu, Japan)) for 16 h ...
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bioRxiv - Bioengineering 2024Quote: ... a new 24-well non-treated tissue culture plate was coated with 10 µg retronectin (Takara, T100A) in 1 ml PBS per well ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was isolated from 1 µg total RNA by polyA selection (PrepX polyA 48, Takara Bio 640098) and checked for rRNA contamination using the mRNA 2100 Bioanalyzer chip and protocol (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells (Clontech #11131D) with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection was carried out using Lenti-X Packaging Single Shot (VSV-G) system (Takara Bio, 631275) according to the following protocol ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells (Clontech #11131D) with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606) ...
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bioRxiv - Immunology 2024Quote: ... The substitution G>A into the ISRE consensus sequence was introduced by In-Fusion cloning (TaKaRa). The promoter region was amplified in two fragments ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA samples and DNA standards were then amplified using One Step PrimeScript™ RT-PCR Kit (TAKARA BIO INC, Kusatsu, Shiga, Japan) and the following primer set ((Forward ...
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bioRxiv - Microbiology 2021Quote: ... RNA-free cDNA (5μL) was used as a template for PCR with PrimeStar GXL Polymerase (Takara, Japan) and back-to-back PCR primers (LigSeq-F and LigSeq-R ...
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bioRxiv - Bioengineering 2021Quote: ... an endotoxin-free plasmid midiprep DNA purification took place using NucleoBond Xtra Midi EF kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HuEpi library was sequenced and contains all 5,309 sgRNAs included in the synthesis (GSE215430).
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Molecular Biology 2024Quote: ... Pierce Protease Inhibitors EDTA-free (PIA32955) and Pierce Phosphatase Inhibitors (PIA32957) and RNase inhibitor (TaKaRa, cat# 2313A)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Pierce Protease Inhibitors EDTA-free (PIA32955) and Pierce Phosphatase Inhibitors (PIA32957) and RNase inhibitor (TaKaRa, cat# 2313A)) for 10 minutes on ice ...
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bioRxiv - Biophysics 2024Quote: Cells were grown in DMEM culture media (Thermo-Fisher) supplemented with 10% tetracycline-free FBS (Clontech, 631106), 1% sodium pyruvate (NaPy ...
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bioRxiv - Molecular Biology 2020Quote: A549 cells were obtained from ATCC and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Takara Biomedical Technology Co., Ltd., China), 100 U/ml penicillin and 100 U/ml streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... using each of the following 4 restriction enzymes: HaeIII or Hha I or Rsa I or Alu I (10 units, Takara Bio Co. Ltd. Shiga, Japan) in buffer solution (10xLow salt buffer ...
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bioRxiv - Microbiology 2024Quote: ... Mycelia were separated from broths via 2 layers of lens paper (10×15 cm, NEWSTAR Industry, Hangzhou, China) and collected for DNA extraction by using DNAiso Reagent (TaKaRa Biotechnology Co. Ltd, Dalian, China) as described in the manual ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase, TAKARA) and QPCR was performed using TaqMan™ Gene Expression Master Mix (4369016 ...