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Citations for Takara Bio :
451 - 500 of 747 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: AAV vectors (serotype DJ) were generated using the pHelper (AAVpro Helper-free system, Takara), pAAV-DJ (Cell Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa-KRAS(G12V) cells were maintained in 10% tetracycline-free FBS (Takara Bio, 631101) and KRAS(G12V ...
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bioRxiv - Cell Biology 2022Quote: ... and the samples were homogenized using an RNase-free tube and plunger (Takara, 9791A) and then vortexed for 5 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were grown in HEK293T medium except 10% tetracycline-free FBS (Takara Bio) was used ...
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bioRxiv - Immunology 2024Quote: ... and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio). For immunocytokines ...
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bioRxiv - Cell Biology 2023Quote: ... Greenberg) were cultured in DMEM supplemented with 10% tetracycline-free fetal bovine serum (Takara) and antibiotics ...
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bioRxiv - Biophysics 2024Quote: ... and pH 7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The suspended solution was put on ice and homogenized using a sonicator (Qsonica ...
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bioRxiv - Immunology 2024Quote: ... the RNA samples were treated with RNase-free DNase I (Takara Bio, Shiga, Japan) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed in triplicate using 1 µL of cDNA in a standard SYBR premix Ex Taq (Takara, Shiga, Japan) on the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... The plasmid was linearized using AgeI and SalI restriction enzymes and inserts were cloned to the linearized plasmid in 17 reactions using the standard InFusion cloning (Clontech) protocol ...
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bioRxiv - Microbiology 2020Quote: ... All elements of the DiCre plasmid were amplified by PCR using standard PCR conditions (using the CloneAmp HiFi PCR premix) and sequentially ligated (In-Fusion HD Cloning Kit, Clontech) into an acceptor plasmid containing a TgDHFR/TS cassette flanked by two LoxP sites ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was set for prepared cDNA and serially diluted standard (copy number was calculated and diluted from 108 to 103) using SYBR Green mastermix (Takara) and HCV specific PCR primers in LightCycler 480 instrument (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Microbiology 2024Quote: ... was performed via standard and overlapping PCRs with CloneAmp™ HiFi PCR Premix (Takara Bio, cat # 639298, San Jose, CA), with subsequent insertion into the construct at unique restriction sites by In-Fusion ligation- independent cloning (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR test was performed in a Roche 480 Lightcycler with a 2x SYBR-green master mix using standard protocol instructions (Takara). Total Wolbachia density was analyzed by relative quantification of the Wolbachia surface protein against the mosquito homothorax gene ...
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bioRxiv - Immunology 2024Quote: ... Lentiviral particles were packaged in HEK293T cells following standard protocols and concentrated 10x using the Lenti-X Concentrator reagent (Takara). Viral particles were added to T cells at low multiplicity of infection ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (1 µg) was reverse-transcribed with PrimerScript™ RT Master Mix (Takara, #RR047A) after DNase I treatment (Takara ...
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bioRxiv - Neuroscience 2022Quote: 50 µg of human cerebral cortex lysate (Protein Medley, Takara, Kusatsu, Japan, Cat. No. 635323) were diluted and prepared according to manufacturer’s instructions (protocol PT1602-1) ...
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bioRxiv - Genetics 2024Quote: ... wherein 2.5 µg of pooled plasmid was diluted with Xfect reaction buffer (Takara Bio, 631318) to a total volume of 50 μL ...
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bioRxiv - Biochemistry 2024Quote: ... DNaseI-treated total RNA (4 µg) was reverse transcribed using Primescript reverse transcriptase (Takara Bio) and random hexamers (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of total RNA was converted to cDNA using M-MLV reverse transcriptase (TAKARA) under standard conditions with oligo(dT ...
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bioRxiv - Cell Biology 2023Quote: ... Contaminant DNA in 50 µg of extracted RNA was digested with DNaseI (cat. #2270A, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 2 µg of total RNA using PrimeScript RT Master Mix (Takara), and expression of the indicated genes was analyzed by quantitative RT-PCR using the CFX Opus 96 Real-Time PCR System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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bioRxiv - Developmental Biology 2021Quote: ... Then, they were cloned into pmirGLO vector (Wuhan GeneCreate Biological Engineering Co., Ltd., Wuhan, China) between NheI and XhoI (TaKaRa, Nojihigashi, Japan), and the empty plasmid was transfected as the control group ...
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bioRxiv - Neuroscience 2021Quote: Total mRNA was extracted from the hypothalamus using a TRIzol reagent according to the manufacturer’s instructions (Takara Biotechnology Co. Ltd, Dalian, China). RNA was reverse transcribed into cDNA using a cDNA reverse transcription kit (Catalog no ...
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Plant Biology 2020Quote: ... while specific AD and BD recombinant plasmid pairs were used to co-transform yeast strain AH109 cells according to the Yeast Protocols Handbook (Takara, Dalian, China).
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bioRxiv - Plant Biology 2022Quote: ... The DNA encoding the Piks-HMAs in pGBKT7 was co-transformed with either the individual AvrPik variants in pGADT7 into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). The single colonies grown on selection plates were inoculated in SD-Leu-Trp plate and grown at 30 °C for overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... either AML cells were co-cultured with lentiviral particles at a seeding density of 1×106 cells per mL in a Retronectin dish (Takara Bio; T110A). HCT116 cells were seeded at a density of 0.2×106 cells per mL in a six well plate ...
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bioRxiv - Plant Biology 2024Quote: ... The relevant vectors were co-expressed in Y1H yeast cells and screened with varying concentrations of Aureobasidin A (AbA, TaKaRa, Beijing, China).
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bioRxiv - Microbiology 2024Quote: ... the cells were co-transfected with 500 ng of pCAGGS-mNeonGreen-X using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) in the Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cells and lentiviral particles were co-cultured at a seeding density of 1×106 cells per mL in a Retronectin dish (Takara Bio; T110A). Following 72 hours of infection ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentiviral supernatants were produced using Lenti-X Packaging Single Shots (VSV-G) (Clontech #631275) mixed with pLKO.1 puro-based lentiviral constructs transfected to Lenti-X 293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... and a vesicular stomatitis virus glycoprotein (VSV-G)-expressing plasmid (Clontech, Palo Alto, CA), pVSV-G [43] ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Genomics 2021Quote: ... U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech), 100U/ml penicillin and 100µg/ml streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Immunology 2024Quote: ... the isolated RNAs underwent treatment with RNase-free DNase I (Takara Bio, Otsu, Shiga, Japan) to remove any contaminating DNA ...
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bioRxiv - Immunology 2023Quote: ... The RNA samples were dissolved in RNase-free water (TAKARA HOLDINGS Inc., Kyoto, Japan, 9012) and measured with a spectrophotometer (NanoDrop ...
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bioRxiv - Plant Biology 2021Quote: ... we used the nuclear system Y2H mating standard operating procedure method as described in the manufacturer’s instructions (Clontech Laboratories, Inc., USA) to screen the protein that interacts with TiAP1 and further verified the obtained interaction protein ...
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bioRxiv - Cell Biology 2022Quote: All plasmid fusions assembled in-house were generated through standard ligation-based methods using the In-Fusion cloning kit according to manufacturer protocols (Takara, 638920). MBNL1 RNA-interaction and chelation mutant sequences were designed based on plasmids generated by Andrew Berglund (Purcell et al. ...
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bioRxiv - Microbiology 2022Quote: ... into which all possible combinations of 36 assays and 144 samples (127 woodchip samples, 16 standards, and one no-template control) were loaded by the SmartChip MultiSample NanoDispenser (Takara Bio). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... The copy number was calculated using the standard curves for cloned Tert and Dloop in a pMD19-T Easy vector (TaKaRa, 3271), respectively ...
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bioRxiv - Neuroscience 2021Quote: 8 DIV (days in vitro) neurons were transfected with 1.5 µg of pEGFP-N1 vector (Clontech Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... hLAG3 expression was then induced by addition of 1 µg/ml of Doxycycline (DOX; Clontech #631311) 3 weeks (4 week DOX on conditions ...