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Citations for Takara Bio :
351 - 400 of 747 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... CydDC was purified via His6-tag affinity chromatography using a Talon (Co-IMAC) affinity matrix (Takara Bio Inc.). For column conditioning and washing step I (10 column volumes [CV] ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with the LentiX VSV-G transfection mix (Clontech) and different SARS-CoV-2 ORFs (cloned into pLX vector) ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and pCMV-G using Calphos transfection reagent (Clontech, Takara, Jusatsu, Japan). Medium was changed 16 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... and pCMV-G using Calphos transfection reagent (Clontech, Takara, Jusatsu, Japan). Medium was changed 16 hours after transfection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with standard (DreamTaq - Thermo Fisher Scientific, or EmeraldAmp Max HS PCR Master Mix - TaKaRa Bio Inc) DNA polymerases ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1, Takara, Otsu, Japan). For the transformation ...
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bioRxiv - Biochemistry 2022Quote: ... aIF1A was purified as described (24) except that a standard affinity chromatography step on Talon resin (Clontech) was added to the original protocol ...
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bioRxiv - Microbiology 2020Quote: ... Western blotting was performed using standard procedures with the following primary antibodies: JL-8 monoclonal antibody (Takara) for GFP variants ...
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bioRxiv - Molecular Biology 2020Quote: DNA ligation was performed using standard ligation techniques (Takara DNA Ligation kit ver. 2.1; Takara, Otsu, Japan). For transformation ...
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bioRxiv - Bioengineering 2020Quote: Expression constructs were cloned using standard PCR methods and In-Fusion® HD Cloning (TaKaRa Bio Europe). Primers were ordered from Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcriptase-containing pseudoviral particles and recombinant reverse transcriptase standard of known concentrations (TAKARA, Cat. No. RR047A) were 10-timed diluted with nuclease-free water (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Standard immunoblotting procedures were performed using a primary antibody against GFP (mouse monoclonal JL-8 antibody, Takara) and a secondary antibody goat anti-mouse IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... The amount of p24 was determined by comparison to serial dilution to a p24 standard (Takara Biosciences). The number of p24 per EDV was approximated to be 2500 CA molecules per particle (47).
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bioRxiv - Neuroscience 2020Quote: ... and 0.3% Triton X-100 in PBS and then stained with a primary antibody for 48 hours at 4°C with agitation in blocking buffer: DsRed (anti-rabbit, 1:5000, cat. number NC9580775, Takara), GFP (anti-chicken ...
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bioRxiv - Immunology 2021Quote: ... The extracted RNAs were treated with RNase-free DNase I (TaKaRa, Japan) to remove contaminating genomic DNAs ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating sections were immunostained for mCherry (Cat. #632543, TaKaRa Bio Europe) using the Avidin-Biotin Complex Peroxidase (ABC-P ...
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bioRxiv - Plant Biology 2020Quote: ... RNA samples were treated with DNase I (RNase-free) (Takara-cat # 2270B) to remove residual genomic DNA ...
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bioRxiv - Pathology 2023Quote: ... and subsequently treated with RNase-free DNase I (Takara Bio, Dalian, China) as instructed by the manufacturers ...
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bioRxiv - Microbiology 2023Quote: ... Free DNA was removed via DNase treatment (Recombinant DNase I; Takara, Japan). Viral DNA was extracted using the DNeasy Blood & Tissue Kits (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... and media was exchanged for DMEM with 10% Tet-free FBS (Clontech). Supernatants were collected and 0.45 μm filtered at 48 and 72 hours post-transfection and immediately used for infection of MeWo-rtTA target cells in the presence of 8 μg/ml of polybrene (Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... The sorted cells were returned to DMEM with TET-free FBS (Takara). MDA-MB-231-mARGAna was sorted twice ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FBS, TaKaRa Cat. #631368), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: Cloning and mutagenesis were performed using In-Fusion restriction-free cloning (Takara). Reporter gene constructs were cloned by inserting PCR amplified promoter elements into the pPD95.75 vector (Fire Vector Kit) ...
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bioRxiv - Molecular Biology 2021Quote: ... was co-transformed with GAL4 DNA-BD-fused IPMK and a human brain cDNA activation domain (AD) library (Clontech). Two different reporter genes (HIS3 and ADE2 ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed into cDNA using Takara PrimeScript RT master mix (RR036A; Takara Biotechnology Co., Ltd., Dalian China). RT-qPCR was performed using an ABI StepOne Plus Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: RPE1 cells co-expressing 53BP1-eGFP and Lifeact-RFP were generated using two individual pIRES-Neo3 vector systems (Clontech) harboring either the coding sequence of 53BP1 (representing amino acid 1220-1711 ...
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bioRxiv - Plant Biology 2021Quote: ... The two types of plasmids were co-transformed into yeast strain EGY48 according to the Yeast Protocols Handbook (Clontech) as described previously(Lin et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Each individual effector-HMA combination was then co-transformed into competent Saccharomyces cerevisiae Y2H Gold cells (Takara Bio USA) and selected on SD-Leu-Trp (synthetic dropout ...
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bioRxiv - Bioengineering 2022Quote: ... A lentiviral transfer plasmid co-encoding zsGreen with Oatp1b1 was cloned using the In-Fusion HD Cloning kit (Takara Bio USA Inc ...
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bioRxiv - Plant Biology 2021Quote: ... Bait (pGBKT7) and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 according to manufactural instruction (Clontech). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were co-transfected with the aforementioned lentiviral vector plasmid and Lentiviral High Titer Packaging Mix (Takara Bio).
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bioRxiv - Molecular Biology 2022Quote: ... pSiCBL4::LacZ or pSiCBL7::LacZ were co-transformed into yeast strain EGY48 according to the Yeast Protocols Handbook (Clontech). Transformed yeast (Saccharomyces cerevisiae ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Physiology 2024Quote: ... Using a Bio-rad Mx3000P qPCR system and SYBR Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa Biotech. Co.), qRT-PCR analyses were carried out ...
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bioRxiv - Plant Biology 2024Quote: ... The respective combinations of GAD-fusion and GBD-fusion plasmids were co-transformed into yeast strain Y2H-Gold (Clontech) and colonies grown on synthetic defined (SD ...
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bioRxiv - Cell Biology 2021Quote: ... 200 MOI retrovirus and 5 µg/cm2 RetroNectin reagent (Takara, Cat. No. T100A) were used in the transduction following the manufactory protocol ...
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bioRxiv - Neuroscience 2021Quote: ... hLAG3 expression was induced by 1 µg/ml of Doxycycline (DOX; Clontech #631311).
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bioRxiv - Cell Biology 2020Quote: Plasmids were constructed using standard molecular biology methods including polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific residues were introduced by overlap-extension PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories). For two-hybrid experiments in meiotic conditions ...
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bioRxiv - Microbiology 2022Quote: All plasmid inserts were amplified by PCR using standard PCR conditions and the CloneAmp HiFi PCR premix (Takara). Following a PCR purification step (QIAquick PCR purification kit) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid constructions were performed with standard restriction enzyme cloning or In-fusion HD Cloning kit (Takara Bio USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories).
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bioRxiv - Plant Biology 2021Quote: ... transferred to PVDF membranes by Western blotting and visualized with anti-GFP (JL-8 Takara Bio Clontech, Lot #A8034133, 1:5000), anti-α-tubulin (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... transferred to PVDF membranes by Western blotting and visualized with anti-GFP (JL-8 Takara Bio Clontech, Lot #A8034133, 1:5000), anti-α-tubulin (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was spun at 5000 rpm for 10 min to remove cell debris and concentrated via Lenti-X as per the instructions (Takara Bio). HIV-Gag-iGFP_JRFL (the following reagent was obtained through the NIH HIV Reagent Program ...
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bioRxiv - Immunology 2021Quote: ... pMD2.G and the CAR-expression plasmid by calcium phosphate transfection (Clontech). At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... using Takara’s Lenti-X (VSV-G) Packing Single Shots (Takara Bio/ Clontech Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmids using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmid with TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM (Thermo Fisher Scientific ...