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Citations for Takara Bio :
351 - 400 of 1429 citations for BFF 122 CAS 1152314 49 2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... FcγRIIA cDNA was cloned into a pEGFP-N vector (Takara Bio USA, CA). FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma ...
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bioRxiv - Evolutionary Biology 2019Quote: ... These PCRs were performed with the Advantage2 Taq Polymerase (Clontech, Mountain View, CA) for high fidelity ...
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bioRxiv - Biochemistry 2020Quote: ... Insertions and deletions were introduced using In-Fusion cloning (Takara, Mountain View, CA).
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bioRxiv - Immunology 2020Quote: ... AP-1-Luc or NF-IL6-Luc plasmid (Clontech, Pal Alto, CA, USA) using Lipofectamine 2000 for 24 h (Stratagene ...
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bioRxiv - Cell Biology 2020Quote: ... except the GFP expression vector pEGFP_C2 (BD Biosciences Clontech, San Jose, CA, USA), were derived from vector pAdRSV-Sp (Giudicelli et al ...
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bioRxiv - Physiology 2021Quote: ... primers included oligonucleotide overhangs for In-Fusion Cloning (Clontech, Mountain View, CA, USA) into a pCR2.1-TOPO vector (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... pGBKT7-Lam and pGBKT7-p53 were purchased from Clontech (Mountain View, CA, USA). Yeast strain AH109 was co-transformed with the combination of the pGADT7 and the pGBKT7 plasmids ...
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bioRxiv - Neuroscience 2020Quote: Cytotoxicity was measured using lactate dehydrogenase (LDH) kit (Clontech; Mountain View, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: Human NIC4 was sub-cloned into pEGFP-N3 (BD Clontech, Mountain View, CA) between EcoRI and BamHI restriction sites to obtain NIC4-GFP using the following primers:
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180; Mountain View, CA). These cells were maintained in DMEM (no ...
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bioRxiv - Microbiology 2021Quote: ... then ligating both PCR products by In-Fusion (Takara Bio, Mountain View, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using the Apollo NGS library prep system (Takara Bio, Mountain View, CA, USA). Libraries were evaluated for quality using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X human embryonic kidney cell line (Clontech, Mountain View, CA, USA) was used for LV or IDLV production by transient transfection as previously described22,58 ...
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bioRxiv - Immunology 2020Quote: ... Supernatant was then incubated overnight with Lenti-X concentrator (Clontech, Mountain View, CA) at a ratio of 1:3 ...
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bioRxiv - Microbiology 2020Quote: ... Following PCR amplification with TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA), the PCR product and pRRK plasmid were digested with the XBaI restriction endonuclease (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: We performed PCR with CloneAmp HiFi PCR Mix (Takara Biosciences, Mountain View, CA) and added DMSO to a final concentration of 5-10% (v/v ...
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bioRxiv - Immunology 2024Quote: ... cDNA was prepared using the Ultra Low kit from Clontech (Mountain View, CA). Libraries were prepared at the according to manufacturer’s instructions for the NEBNext Ultra II Direction RNA kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... concentrated 10x using the Lenti-X lentivirus concentrator (Clontech Laboratories, Inc. Mountainview, CA), resuspended in dPBS+/+ ...
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bioRxiv - Cell Biology 2023Quote: ... which was cloned into the pEGFP-C3 vector (Clontech, Mountain View, CA, USA) using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Neuroscience 2024Quote: ... genes of interest were amplified using a PrimeSTAR GXL kit (Clontech, CA, USA). The amount of cDNA was normalized according to β-actin2 amplification in qRT-PCR and RT-PCR experiments ...
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bioRxiv - Genomics 2024Quote: ... Lentiviral particles were produced in HEK293T Lenti-X cells (Clontech, Mountain View, CA). Cells (5×106 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were supplemented with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA) and 1 mM neuraminic acid (Neu5Ac ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were induced with 0.5 μg·ml−1 anhydrotetracycline (aTc) (Clontech, Mountain View, CA). When experimental conditions required it ...
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bioRxiv - Plant Biology 2024Quote: ... from plasmids pFny209 (see below) or pGFP-C1 (Clontech laboratories, Mountain View, CA), into the BpiI-digested modified pUC18 ...
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bioRxiv - Genomics 2019Quote: The 90-μL preparation volume for PCR contains 1X Advantage 2 PCR Buffer [not short amplicon (SA)](10X, Clontech, 639206, Advantage® 2 PCR Kit), 0.4-mM dNTP Mix (50X/10 mM ...
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bioRxiv - Plant Biology 2020Quote: ... The transformants were grown on SD -Trp plates (Clontech, 2% agar) for selection ...
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bioRxiv - Developmental Biology 2020Quote: ... Laminin-511 E8 fragment (Takara Bio, #T303, 0.05 – 2 µg/ml) and Laminin-521 (Biolaminin ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was applied to 2 ml Talon Superflow resin (Clontech) pre-equilibrated with buffer A (20 mM Tris/HCl pH 7.5 ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... PCR amplification was done using Advantage HF 2 DNA polymerase (Takara) for 30 cycles according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: The GAL4-based Matchmaker yeast 2-hybrid system (Clontech Laboratories Inc.) was used for yeast-two-hybrid analysis ...
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bioRxiv - Cancer Biology 2022Quote: U-2 OS Tet-On cells were purchased from Clontech (#630919). MDA-MB-453 cells were purchased from the American Type Culture Collection (ATCC ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Microbiology 2020Quote: ... HSV-1 was pretreated with 2 μg/ml DNase (Takara, Japan), and then diluted to MOI=20 ...
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bioRxiv - Plant Biology 2021Quote: ... Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA) was used for the process of yeast transformation ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was nutated with 2 mL of TALON resin (Takara) for 45 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Colony PCR reaction contained 10 μl 2×La Taq Mix (Takara), 1 μl colony ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl 2×SYBR®Premix Ex Taq™ II (TaKaRa), 0.75 μM primers and nuclease-free water to 20 μl ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of 5× PrimeSTAR GXL Buffer (Takara Bio, Kusatsu, Japan), 1.0 μL of PrimeSTAR GXL DNA Polymerase (1.25 U/μL) ...
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bioRxiv - Plant Biology 2024Quote: ... equilibrated with 2 μg of anti-GFP antibody (Takara Bio, 632381). Beads were washed for 2 x 10 mins in low salt wash buffer ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 1x Titanium Taq buffer and 2 μL Titanium Taq polymerase (Takara) were mixed in 50 µL total volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... which were carried out by Advantage® 2 Polymerase (Takara Bio). Final products were outsourced for Sanger sequencing (HyLabs ...
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 2 ug Retronectin (TaKaRa Cat. no. T110A), 1 ug anti-mouse CD11a (LFA1) ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in Mesenchymal Stem Cell Growth Medium 2 (TaKaRa Bio) supplemented with penicillin/streptomycin/amphotericin B (Wako) ...
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bioRxiv - Genetics 2021Quote: ... The clarified lysate was subjected to Talon Metal Affinity column (Takara, Mountain View, CA) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the Takara LA HS Taq long range polymerase (Takara, Mountain View, CA, USA). PCR conditions used were 94°C for 3 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... transfected at 5-7 DIV with the plasmid peGFP-N1 (Clontech, Mountain View, CA) using lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara Bio, Mountain View, CA, USA). CasRx-GFP lentiviral particles were produced by co-transfecting plasmids encoding CasRx-GFP (PXR001 ...
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bioRxiv - Molecular Biology 2021Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara Bio, Mountain View, CA, USA). To obtain cells with stable CasRx-GFP expression ...
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bioRxiv - Molecular Biology 2020Quote: ... The pHISi-1 vector was provided by the Matchmaker one-hybrid kit (Clontech, CA). The 40 bp promoter sequence was originally cloned from the maize C1 gene promoter (22) ...