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Citations for Takara Bio :
4351 - 4400 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... We introduced a T44C mutation and 108C mutation into the GB1-loop by site-directed mutagenesis using a PrimeSTAR mutagenesis basal kit (Takara Bio).
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bioRxiv - Cell Biology 2024Quote: ... GS linker human IgG1 FC region from pcDNA3-sACE2v2-Fc (IgG1) and PGK promoter puromycin resistance region cloned into lenti CAG-FLAG-dCas9-VPR using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Pathology 2024Quote: Expression for IFN-related genes was conducted by reverse transcription using the PrimeScript RT Reagent Kit (Perfect RealTime, Takara Bio Inc.). qPCR reaction was performed in duplicate using Takyon ROX SYBR MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Genomics 2024Quote: ... 152 ∗106 GPRTG cells were seeded into a 2-STACK culture chamber (Cornig) and transfected 24 h later with 238 µg transfer plasmid using the CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Then the RNA-seq library was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit (TaKaRa Bio, USA). This was performed in Shpokayte et ...
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bioRxiv - Immunology 2024Quote: ... The damaged lung areas of the infected mice and the corresponding lung areas of the uninfected control mice were excised to isolate total RNAs using TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, 9767), which were reverse transcribed into cDNAs using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
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bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μg DNA-free total RNA was used the template for synthesizing the first strand cDNA (PrimeScript II 1st Strand cDNA Synthesis Kit; Takara, Dalian, China). The primers of EcaICE1 and EcaHOS1 (listed as table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Library generation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (cat no. 634412, Takara Bio Inc), then submitted to the MIT BioMicro Center for quality control (fragment analysis and qPCR) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
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bioRxiv - Cell Biology 2020Quote: ... Point mutants were generated by site-directed mutagenesis using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). Sequence correctness was verified by sequencing (LGC Genomics ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... The above four fragments plus the fragment of the gentamycin resistance cassette flanked by loxp sites were connected and inserted into the linearized plasmid pUC19 using the In-Fusion Cloning Kit (TaKaRa, Daliang, China), generating the plasmid pUC-GSP ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was combined with fragment 2 and the gentamycin resistance cassette flanked by loxp sites and then inserted into the linearized plasmid pUC19 by an In-Fusion Cloning Kit (TaKaRa, Daliang, China) to generate the plasmid pUC19-Gent ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA isolated from each condition was converted into cDNA and processed through the SMARTer® 5’/3’ RACE Kit (Takara Bio) for 5’-RACE following manufacturer protocols ...
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bioRxiv - Microbiology 2019Quote: ... producing pET30a IN-6xHis (INHis) or pET30a 6xHis-CCD (CCDHis) by using In-Fusion® HD Cloning Kit (TAKARA Bio Inc., Japan). Flag-tagged IN (F-IN ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 ul from each samples were directly used for cDNA amplification (15 cycles of LD PCR, using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio, #634898) and the SeqAmp DNA Polymerase (Takara Bio #638509) ...
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bioRxiv - Developmental Biology 2021Quote: ... Pdum-Lox2 and Avi-Post2 the SMART™ (Switching Mechanism At 5’ end of the RNA Transcript) RACE method (SMART™ RACE cDNA amplification kit, Clontech) was used (Brena et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... templates were constructed by cloning the 2 kb 5’ and 3’ homology arms into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). We used the CRISPR design tool (http://crispr.mit.edu ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Genomics 2020Quote: ... total RNA of midgut samples in treatment groups and control groups were respectively extracted using AxyPrep™ Multisource Total RNA Miniprep Kit (TaKaRa, Japan); (2 ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR products were nested PCR-amplified with primers for IgG VH or IgG VL following the HS DNA polymerase kit protocol (Takara, TAK R010). The IgG VH and IgG VL nested PCR products were purified and next cloned into human IgG1 heavy chain and light chain expression vectors ...
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bioRxiv - Microbiology 2022Quote: ... A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 µL reaction ...
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bioRxiv - Neuroscience 2021Quote: ... 2 days post-treatment the vehicle control flasks had DNA from 1×108 cells per replicate isolated with Nucleobond CB 500 kit (Takara Bio 740509) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified using specific primers (forward: 5’-AAGGAGATATACATATGCTCTCCGAAATGGTGGAAGAAG-3’; reverse:5’-GTGCGGCCGCAAGCTTTTATTTCTTTTTGTTGGTGGTCTG-3’) and cloned into pET28a using an InFusion HD Cloning Kit (Takara Bio USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries for HEK293T samples were generated using the SMARTer Stranded RNA-Seq Kit which uses a template switching reverse transcriptase (Takara Bio 634837). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified viral RNA (vRNA) was reverse transcribed into cDNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan). The number of RV genome copy in the medium supernatant was determined using RT-qPCR with general or serotype-specific Taqman probe and primers (Additional file 2 ...
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bioRxiv - Genomics 2019Quote: The first-strand cDNA was synthetised from 1 μg of 5 tissues (root, stem, leaf, flower and peel) total RNA using Prime Script RT Reagent Kit (Takara, Dalian, China). The qRT-PCR reactions were performedin 96-well plates using the ABI 7500 fast Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The same samples used for RNA sequencing were used for reverse transcription and synthesis of cDNA with PrimeScript RT Reagent Kit with gDNA Eraser following the instructions of the manufacturer (TaKaRa, Dalian, China). For designing the primers for qRT-PCR ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... These four purified products were introduced to the HindIII and BamHI sites of PKNTG using In-Fusion-HD cloning kit (Takara Bio USA). The plasmid was then sequenced and introduced into the ΔFvsec4 strain for genetic complementation and localization study.
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bioRxiv - Developmental Biology 2019Quote: ... The SOX2 PCR product was purified and inserted into CMV-t2a-GFP using the In-Fusion HD cloning kit (Takara Bio USA) and transformed into Stellar Competent Cells (Takara Bio USA) ...
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bioRxiv - Immunology 2019Quote: ... by generating cDNA from 2 ng RNA and 9 PCR cycles per replicate with the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (cat# 634891, Takara) and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096 ...
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bioRxiv - Molecular Biology 2019Quote: ... LINC00662 (NR_027301) and ELK4 (NM_001973.4) were detected by One-Step SYBR PrimeScript RT-PCR Kit (Perfect Real Time; Takara Bio, Inc., Kusatsu, Japan). Relative expression values were calculated using the relative quantification (2−△△Ct ...
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bioRxiv - Plant Biology 2019Quote: ... 1μg purified total RNA was used to obtain the first-strand cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Japan). The Takara SYBR ® Premix Ex Taq™ II was used to perform the qRT-PCR assay using primers P13 and P14 ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified using Nucleo Spin® Gel and PCR Clean-up Kits (Code No. 740609.50; TAKARA Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA (1 µg) was reverse transcribed into First-strand complementary DNA using a PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions and stored at –20°C.
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... the PC1-CTT (mPC-4119) [6] insert was fused with mCherry and cloned into pcDNA3 using an In-Fusion HD Plus kit (Takara Bio, 638909). The expression plasmids for EGFP-SLK (pEGFP-C1+SKL ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Cell Biology 2021Quote: ... or mCherry cDNA were cloned at the position next to S252 of DFNA5 cDNA using an In-Fusion HD cloning kit (TaKaRa, Seoul, Korea). All the lentiviral vectors ...
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bioRxiv - Biochemistry 2020Quote: Complementary DNA (cDNA) was generated from previously extracted total RNA (900 ng) using the SMARTER RACE 5’/3’ kit (Takara Bio USA) according to the manufacturer’s instructions ...