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Citations for Takara Bio :
4301 - 4350 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... This clone was digested with NsiI and BglII to remove the CMV promoter sequence and was combined with the pSV010(-) SV40 promoter fragment using the Infusion® HD Cloning kit (Clontech) such that the pSV010(- ...
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bioRxiv - Cell Biology 2023Quote: ... and constructs were isolated from 50 ml bacterial cultures using a plasmid DNA extraction kit (Takara Bio USA, Inc. NucleoSpin Plasmid). DNA concentrations were measured by a NanoDrop spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... Two micrograms of the RNA were subjected to reverse transcription with random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). For semi-quantitative PCR ...
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bioRxiv - Neuroscience 2023Quote: ... total RNAs were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara, #RR037A) according to manufacturer’s instructions in the presence of a synthetic external non-homologous poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the total RNA was used for cDNA synthesis using the Prime Script 1st Strand cDNA Synthesis Kit (TaKaRa, 6110A). Random 6-mer primers (0.3 µL ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was prepared from the extracted RNAs with oligo (dT) and random primers using the PrimeScript RT reagent kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
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bioRxiv - Genetics 2023Quote: First-strand cDNA was synthesized from 5 μg of total RNA using the PrimeScriptTMII cDNA Synthesis kit from Takara (https://takara.com/). The full-length cDNAs of SUH1 and BC10 were amplified using the Taq LA DNA polymerase PCR kit from Takara (https://takara.com/ ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from all the MBS and soil samples using the NucleoSpin Soil DNA extraction kit (Takara Bio, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The library inserts from last step were ligated to the linearized STARR-seq vectors using In-Fusion HD Cloning Kit (Clontech 639650) following the manufactory’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Three RNA lysis aliquots of the LC6 sample were processed and library preparation completed on the 3 aliquots with SMART-Seq® HT Kit (Takara) and Illumina Nextera reagents for tagmentation ...
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bioRxiv - Cell Biology 2023Quote: Plasmids NLAD8 HIV-1 AD8 Macrophage-Tropic R5 (NIH AIDS reagents #11346) or HIV Gag-imCherry V3 loop (29) were transfected in HEK 293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) in DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... complementary DNA (cDNA) was synthesized from the total RNA samples using the TaKaRa PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, 6110A).
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bioRxiv - Cancer Biology 2023Quote: Reverse Transcriptase PCR and Real-Time PCR Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A quantity of RNA in the range 200 – 500 ng was used for cDNA synthesis using the PrimeScript RT-PCR Kit (Takara-Bio). Quantitative PCR was then performed using Taqman Fast Advanced MasterMix with Universal Probe Library Probes (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: Broccoli RNA template sequences were ordered as dsDNA gBlock as above and inserted into a linearized pRZ plasmid using infusion cloning kit (Takara Bio) and transformed into Stellar cells following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared from these samples using the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara) kit according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... the total RNA of three samples in each group was extracted with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), and linear RNA was then removed with Rnase R (GENESEED ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... the rRNA-depleted and fragmented total RNA fraction was converted to sequencing libraries using the SMARTer® smRNA-Seq Kit for Illumina (Catalog number #635030) from Takara Bio USA ...
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bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was used to synthesize cDNA using the SMART-Seq v4 full-length transcriptome analysis kit from Takara (product # 634888) using protocol B specified in the manual on page 12 ...
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bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc, USA). After shearing of cDNA by Covaris ultrasound water bath sonicator and purification by Ampure beads (Beckman Coulter ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Cell Biology 2024Quote: ... of MMP9 or HSF2 were inserted into the linear vector upstream of the luciferase reporter gene using the In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-7 and ErbB2-overexpressing MCF-7 cells were routinely tested using the CycleavePCR Mycoplasma Detection Kit (Takara bio, CY-232). None of the cell lines were contaminated with mycoplasma.
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for the preparation of libraries using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the amplicons were then ligated into a pUAST-attB vector digested by EcoRI/NotI using In-Fusion HD Cloning Kit (Takara, 639649). The landing site for each construct was {3xP3-RFP.attP}ZH-86Fb ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) following the instructions of PrimeScript RT reagent kit (Takara, Beijing, China). qRT-PCR was performed using the AceQ Universal SYBR qPCR Master Mix (Vazyme) ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng supercoiled plasmid pBR322 obtained from the FastPure Plasmid Mini Kit (Vazyme, DC201-01) or relaxed plasmid pBR322 (derived from supercoiled pBR322 digested by Topoisomerase I, Takara, 2240A), along with 6 μl 5× buffer (170 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... Library preparation of RNA samples was performed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara 634485). Two independent pooled libraries (samples from mice with spontaneous disease and samples from mice with pristane-induced disease ...
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bioRxiv - Immunology 2024Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara, 634888) and 200 pg of cDNA was used to generate sequencing libraries with the NexteraXT kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual genomic and plasmidic DNA was removed using DNA-free kit (InvitrogenTM) and 500 ng of RNA were retro-transcribed with PrimeScript RT Master Mix (Takara Bio) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
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bioRxiv - Molecular Biology 2024Quote: ... Apoptotic cells were quantified via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using the In situ Apoptosis Detection kit (Takara Bio). Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... and cloning reactions were performed at 50 °C for 1 h using the In-Fusion® HD Cloning Kit (Takara Bio). The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Resulting amplicons were mixed with the digested backbone and joined in a single reaction by In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... plasmid and cloned within exon 4 (between base 43-44) and exon 5 (between base 71-72) of the circ-HDGFRP3 sequence by In-Fusion Cloning Kit (Takara Bio) to obtain the final constructs p-circ-Ex4 and p-circ-Ex5.