Labshake search
Citations for Takara Bio :
4451 - 4500 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed with 200 ng total RNA using the Prime Script RT reagent Kit with gDNA Eraser (Takara, Dalian, P.R.China) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... RNA was isolated with Phenol-Chlorophorm and library preparation was carried out with the SMARTer®Stranded RNAseq kit (Takara Bio 634839), according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 3′ and 5′ rapid-amplification of cDNA ends Polymerase Chain Reaction (RACE-PCR) was carried out by using the SMART™ RACE cDNA Amplification Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Low cell RNA-seq libraries (unstranded) wer generated withSMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara/ Clonetech; Cat#634890). All sequencing libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Genomics 2019Quote: ... The Agilent 2100 Bioanalyzer was used to assess RNA quality and only high-quality RNA (RIN > 8) was further processed for cDNA synthesis with SMART-Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized and amplified using SMART-seq® V4 Ultra® Low Input RNA kit for Sequencing (Takara Bio USA, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Libraries from RPFs isolated from HEK293T cells were prepared using the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. No. 635029) and sequenced with 50 cycles single-read on an Illumina NovaSeq 6000 sequencer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelled amplicons were pooled and purified using NucleoSpin Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... 5-AAC TTC GCG CTT CCT AAG TCC CCG AAG GA-3 using Prime STAR mutagenesis kit (Takara Bio Inc. Shiga, Japan). GFP-Rab27a was purchased from RikenBRC(Tsukuba ...
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bioRxiv - Microbiology 2021Quote: ... RNA-Seq library preparation was performed using the SMARTer® Stranded Total RNA-Seq kit – Pico Input Mammalian (Takara Bio USA, Inc) with the RNA input ranging from 7 to 10 ng of starting material.
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bioRxiv - Evolutionary Biology 2020Quote: ... we changed the CaMAL2 promoter to the ScGAL1 promoter using an In-Fusion kit (Takara Bio USA Inc., Mountain View, CA, USA) to generate pSFS4A plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR analysis was performed using the TB Green® Premix Ex Taq™ Tli RNaseH Plus kit (TAKARA, Mountain View, CA). The gene-specific primer sequences were as follows ...
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bioRxiv - Microbiology 2019Quote: ... A pair of primers that match the sr8384 sequence were separately added into the total RNA to synthesize the first-strand cDNA by using the PrimeScript RT Reagent Kit (TaKaRa, cat. #RR047A). Next ...
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bioRxiv - Microbiology 2019Quote: Rapid amplification of cDNA ends (RACE) was performed using reagents from an In-Fusion® SMARTer® Directional cDNA Library Construction Kit (TaKaRa) and custom primers ...
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bioRxiv - Physiology 2019Quote: cDNA library was prepared using SMART-Seq v4 3’ DE kit according to the manufacturer’s instructions (Clontech Laboratories Inc. Cat. No. 635040). For each clone ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time qPCR was performed in 48-well blocks with a StepOne Plus Real Time PCR System by using TaKaRa SYBR Premix Ex Taq II reagent kit (TaKaRa, Tokyo, Japan). All qRT-PCRs were performed in triplicate using total RNA samples extracted from three independent biological replicates ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA samples were diluted to 1 μg/μL and pooled to perform cDNA synthesis by utilizing PrimerScript first strand cDNA synthesis kit (Cat. no. 6110A, Takara Bio, Japan), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... To determine the 5’ and 3’ UTR of the sds3 gene 5’ and 3’ Rapid amplification of cDNA ends (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara Bio) on RNA isolated using Trizol (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
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bioRxiv - Genetics 2020Quote: ... The 5’ and 3’ end of the viral genome was amplified by rapid amplification of cDNA ends by using the 5’ and 3’ Smarter RACE kit (TaKaRa, Dalian, China).
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bioRxiv - Cell Biology 2020Quote: ... Two micrograms of RNA were reverse transcribed into cDNA using a PrimeScript™ First-Strand cDNA Synthesis Kit (TaKaRa, Shiga, Japan; 6110B). Real-time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: Retroviral SARS-CoV-2 Spike pseudovirus were generated in 293T cells by co-transfecting expression plasmids containing SARS-CoV-2 Spike and MLV gag/pol and luciferase vectors using Calphos transfection kit (Takara Bio, USA) as described [20] ...
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bioRxiv - Zoology 2021Quote: ... 1.0 mg RNA was reverse-transcribed into first-strand cDNA using the PrimeScriptTMRT reagent Kit with the gDNA Eraser following the protocol of manufacturer (Takara, Dalian, China). The reverse transcriptional procedure included the following ...
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bioRxiv - Molecular Biology 2019Quote: The 5’ flanking sequence of the insertion sequence was obtained by the Genome Walking Kit according to the manufacturer instructions (TaKaRa, Dalian, China). The random primers were provided by the Genome Walking Kit and the specific primers designed based on theoretical insertion sequences (first round zsp1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The C-terminus of Rtl5 was fused with mCherry by means of a cloning enzyme (In-Fusion® HD Cloning Kit, Takara Bio) and it was inserted into a pGEM T Easy vector (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The Rdl PCR-product was subcloned into the transcription vector pTB-207 38 using the In-Fusion® HD Cloning Kit (Clontech™) and cRNAs were synthesized with the T7 mMessage mMachine kit (Ambion™) ...
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bioRxiv - Pathology 2021Quote: ... The number of SARS-COV-2 copies were quantified using Direct One-Step RT-qPCR Mix for SARS-CoV-2 kit (Takara Bio Inc.).
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bioRxiv - Pharmacology and Toxicology 2022Quote: Site-directed mutagenesis in GC-A and GC-B and construction of chimeric receptors were performed through use of the In-Fusion HD plus cloning kit (Takara Bio Inc.). The primers used are listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Hmx1 putative enhancer elements were amplified by PCR from total genomic DNA using the LA Taq PCR kit v2.1 (TaKaRa, Mountain View, CA) and the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified RNA samples were reverse-transcribed into cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (RR047A, TaKaRa, Tokyo, Japan).
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bioRxiv - Cancer Biology 2021Quote: ... according to instructions provided by the manufacturer and 2μg aliquots were reversed transcribed cDNAs using PrimeScript™ RT-PCR Kit (Takara, Dalian, China). After ...
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bioRxiv - Immunology 2020Quote: ... Viral particle counts were determined by sodium dodecyl sulfate disruption and spectrophotometry at 260 and 280 nm and viral titers were determined using the Adeno-X™ Rapid Titer Kit (Takara Bio). The constructs created included:
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bioRxiv - Microbiology 2020Quote: ... the target genes with or without glycosite mutation were cloned into the pTOR::FLAG vector using the In-fusion HD Cloning kit (Clontech, Beijing, China) (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... and the first strand of cDNA synthesis was performed using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara, Dalian, China). All quantitative PCR were performed in triplicate on an ABI 7500 Fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... the reverse transcription (RT) reaction was performed in a two-step method following the PrimeScript™ II Reverse Transcriptase kit protocol (Takara, Japan). For the first step ...
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bioRxiv - Immunology 2021Quote: ... TM cells were directly sorted into 1X lysis buffer and cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Genomics 2021Quote: Paired-end RNA-seq libraries were constructed from >747 pg of RNA using the SMART-Seq v4 ULTRA Low Input RNA Kit for Sequencing (Takara, cat. 634891) and NexteraXT kits (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... encoding murine preproSST transgene fused with GFP via T2A self-cleavage spacer sequence was prepared by AAVpro purification kit (TaKaRa Bio, Japan) and bilaterally injected into PFC (AP +1.9 mm ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA was synthesized using a mix of random hexamers – oligo d(T) primers and PrimerScript reverse transcriptase enzyme (Takara bio inc. Kit), again following the manufacturer’s instructions ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Total RNA was subjected to reverse transcription according to the protocol of Prime Script 1st Strand cDNA Synthesis kit (Takara Bio USA). Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of tissue specific (ovary) RNA was used for First strand cDNA synthesis using PrimeScriptTM 1st strand cDNA Synthesis Kit (Takara, Cat# 6110A) using the following program ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were cleaved using restriction enzymes and purified using electrophoresis and NucleoSpin Gel & PCR Clean-up kit (Takara bio, Shiga, Japan). The copy number of each standard DNA was calculated based on the concentration quantified with the Qubit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the RNAs of 150µl of the supernatant were extracted and quantified using Retro-X™ qRT-PCR Titration Kit (Takara, cat# 631453), according to the manufacturer instruction.
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bioRxiv - Cancer Biology 2022Quote: ... For reverse transcription 2 µg RNA were translated into cDNA using the “RNA to cDNA EcoDry” Kit (Takara Bio USA, Kusatsu, Japan) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All the fragments were ligated sequentially and inserted into XhoI site on pABpaR2pX by In-Fusion HD Cloning Kit (Clontech Laboratories, Inc.) To generate the transfer vector for NA9-Bac ...
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bioRxiv - Cell Biology 2022Quote: ... 5 ng of total RNA was amplified and converted to cDNA using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech Ref. 634889). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA were amplified from the embryo RNA using PrimeScript™ II High Fidelity One Step RT-polymerase chain reaction (PCR) Kit (Takara, R026A) using the following primers ...
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bioRxiv - Immunology 2022Quote: ... TCR α- and β-chain genes were reverse transcribed and amplified from the RNA using the SMARTer RACE Kit (Clontech Laboratories, Inc). The amplified TCR genes were sub-cloned into the pEF- 1α/pENTR vector (Addgene ...
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bioRxiv - Genomics 2022Quote: We prepared stranded RNA-Seq libraries for each plasma fraction using Clontech SMARTer stranded total RNA-seq kit v2-pico input mammalian (Takara Bio, 634414) according to the manufacturer’s instructions ...