Labshake search
Citations for Takara Bio :
4151 - 4200 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: The total RNA of the two groups (control (LV) and albino mutant (HUA)) was extracted using a Plant RNA Kit (TaKaRa, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... aculeatus ovary and liver cDNA was prepared from total RNA using the SMARTer cDNA synthesis kit (Clontech, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2020Quote: ... The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No. 6025, Takara Bio). The DNA Blunting Kit can smooth DNA with 3ʹ- or 5ʹ- protruding ends ...
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bioRxiv - Microbiology 2020Quote: ... The obtained linearized plasmid was excised from the agarose gel and purified using a MiniBEST Agarose Gel DNA Extraction Kit (Takara Bio). The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No ...
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bioRxiv - Microbiology 2020Quote: ... were used to transcribe poly (A)-tailed miRNAs to generate a cDNA library according to the PrimeScript™ miRNA RT-PCR Kit (Takara) instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR products to be used as DNA templates were cleaned using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio). In vitro transcription was performed using MAXIscript T7 Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: ... The first stand cDNA template was synthesized using the 5′ reagents of the Smarter™ RACE cDNA Amplification Kit (Takara USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were synthesized from 1 μg total RNA using the Prime Script™ RT reagent kit (Perfect Real Time; Takara). All qRT–PCR (quantitative reverse transcription–PCR ...
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bioRxiv - Immunology 2021Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and 500pg of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... approximately 500 sorted cells were processed directly to generate cDNA using the Clontech SMART-Seq v4 Ultra Low Input Kit (Clontech, Inc.) on three biologically-independent replicates ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and reverse transcription and cDNA amplification were performed using 10 ng of RNA using the SMARTer Ultra Low RNA kit (Clontech Laboratories) like previously reported 13 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were cotransfected with hSyn-DsRed and the indicated construct on DIV 7 or DIV 19 (for older neurons) with 1 μg total purified plasmid DNA via CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Genomics 2021Quote: ... and reversely transcribed into cDNA according to the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Japan) manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Double-strand cDNA was amplified by long-distance PCR (LD-PCR) using an Advantage 2 PCR kit (Clontech, cat. no. 639206).
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bioRxiv - Immunology 2021Quote: ... The coding sequences of 14-3-3 and βTRCP were amplified by using cDNAs derived from HeLa cell as templates and inserted into vectors above using In-Fusion HD Cloning Kit (Takara Bio). For Myc-IRAK1 expression vector ...
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bioRxiv - Neuroscience 2021Quote: ... Library preparation was performed by SFGF using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio Inc). The libraries were sequenced on the Illumina HiSeq 4000 platform (2 × 75 bp paired-end reads) ...
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bioRxiv - Neuroscience 2021Quote: mRNA was purified and the cDNA was synthesized with the mRNA fragments as templates using SMART-Seq V4 RNA kit (Clontech # 634889). The sequencing libraries were generated using Nextera XT DNA Library Preparation Kit (Illumina #FC-131-1024) ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were then resuspended in a total volume of 10 µL for library preparation using the SMARTer ThruPLEX DNA-seq library preparation kit (Takara Biosciences) per manufacturer’s protocol with an adjustment on the last step ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was synthesized according to the manufacturer’s instruction of the PrimeScriptTM RT reagent Kit with the genomic DNA Eraser (Takara, Kyoto, Japan). Then ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of total RNA for each sample was reversely transcribed into cDNA by a commercial kit (RR047A, Takara Bio, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... was digested using HinDIII and XhoI to remove the PSMD2 CDS and the amplified Zic3 gene was cloned using Infusion kit (Clontech, USA) as per manufacturer’s instruction. ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (Takara, Shiga, Japan). To amplify cDNA ...
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bioRxiv - Cell Biology 2022Quote: All plasmid fusions assembled in-house were generated through standard ligation-based methods using the In-Fusion cloning kit according to manufacturer protocols (Takara, 638920). MBNL1 RNA-interaction and chelation mutant sequences were designed based on plasmids generated by Andrew Berglund (Purcell et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...
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bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis to construct the variants was performed using the PrimeSTAR mutagenesis basal kit (Takara Bio Inc., Kusatsu, Shiga, Japan) with the pPICZαA vector containing the mature Forham1 gene as the template ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extraction and reverse transcription of RNA into cDNA were performed based on the method described as manufacturer’s protocol of Prime Script TMΠ 1stStrand cDNA Synthesis Kit andTB GreenTM Premix Ex TaqTM II (TaKaRa, Beijing, China). qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
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bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from total RNA using the Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (Takara, Japan), followed by size distribution analysis in the Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent) ...
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bioRxiv - Immunology 2022Quote: Illumina Compatible low input mRNA libraries were prepared using the Smart-Seq V4 Ultra Low Input RNA kit (Takara Bio, USA) and KAPA HyperPlus Library Preparation kit (Roche) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The low-yield gwj02 sample was prepared with the ThruPLEX DNA-Seq Kit (Takara Bio USA, Inc., Mountain View, CA, USA). Sequences (2×250 bp ...
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bioRxiv - Plant Biology 2022Quote: ... purified with Fast pure gel DNA extraction mini kit (DC301, Nanjing Vazyme Biotech, China) and then cloned into the pMD19-T vector (TaKaRa, Japan). The CRISPR-edited sequences were screened by Sanger sequencing of pMD19-T vector.(K ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan). qRT-PCR was performed with Roche LightCycler 480 Real Time PCR system (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The full-length TLN1 was inserted into a pEGFP-N2 vector using the In-Fusion HD cloning kit (Takara Bio USA). Silent mutations on TLN1 were generated with the QuikChange site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... selected RNAs from cDCs were used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). Amplified (1 ng ...
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bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa Bio Inc., Dalian, China) from 1 μg total RNA in a final volume of 20 μL according to the provided protocol ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmid DNAs were isolated using the NucleoBond® Xtra Midi EF plasmid mid-prep kit (Takara Bio USA Inc.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA removal and first-strand cDNA synthesis were carried out using a TAKARA first-strand cDNA synthesis kit (TaKaRa, Japan). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... The genomic fragments were assembled and subcloned into a modified version of pBluescript II KS (StrataGen, pBlueMLAPUCAP) using the InFusion® HD Cloning Plus kit (Clontech), yielding the construct pBS-TPS02r and pBS-TPS02g ...
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bioRxiv - Neuroscience 2022Quote: ... prepared freshly with 940 nL of Lysis Buffer 1 (1 μL of 10X reaction buffer is diluted in 2.75 μL of water) (SMART-Seq v4 Ultra Low input RNA kit; Takara Bio Inc.) per well ...
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bioRxiv - Plant Biology 2022Quote: ... The expression analysis of each target gene was performed quantitatively by real-time RT-PCR method after cDNA synthesis using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Plant Biology 2022Quote: ... and a Gateway® cassette amplified from vector pGWB14 (kindly provided by Pinghua Li, Shandong Agricultural University, Shandong, China) was ligated using the In-Fusion kit (Clontech, USA) for generation the binary destination vector pCSpeGH (Sequence deposited in NCBI Gene Bank with the accession number MW186668) ...
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bioRxiv - Physiology 2023Quote: ... The adenovirus titer in plaque-forming units (PFU) was determined using a Adeno-X Rapid Titer kit (Clontech, Palo Alto, CA).
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from plant tissue at the site of inoculation using a MiniBEST Plant RNA Extraction Kit (TaKaRa, China) as per kit instructions ...