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Citations for Takara Bio :
351 - 400 of 791 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... then we reversed transcription of these RNA to create cDNA by a PrimeScript 1st strand cDNA synthesis kit (Takara, Japan). Fmnl2-EGFP vector was generated by Wuhan GeneCreate Biological Engineering Co ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... The full-length cDNA of the human ZNRF3 was acquired from the human CRC DLD-1 cell line by RNA purification using NucleoSpin RNA II kit (Macherey Nagel) and reverse transcription RT-PCR using specific primers and Primescript RT reagent kit (TaKaRa). Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: We cloned a 1.6 kb genomic region upstream from start codon of mouse Lmna as the endogenous promoter (-1407 to +249 bp from transcription start site) using PrimeSTAR HS DNA Polymerase (Takara). Primers used in this study are listed in Table S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio). Next ...
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bioRxiv - Biochemistry 2020Quote: The full-length L1 fragments of 15 high-risk HPV (hrHPV) were cloned in the pMD plasmid (Takara) to prepare HPV plasmids ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology, Beijing, China), 0.2 µL bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were unroofed after 15 min incubation with 500 nM AP21967 (500 μM stock in ethanol) (Takara, 635055) or 0.1% w/v ethanol (control ...
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bioRxiv - Neuroscience 2020Quote: ... We coated 6-8 mg of 1.6 µm gold beads with 10-15 µg of EGFP-N1 (Clontech) or 20 μg pSuper-cofilin1-shRNA + 20 μg pSuper-ADF-shRNA (Bosch et al. ...
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bioRxiv - Neuroscience 2020Quote: ... and pHelper (Cellbiolabs VPK-401) (per 15 cm plate, 15.5 ug, 21.0 ug, and 33.4 ug respectively) using Xfect (Clontech 631318) transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio). Next ...
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... The cDNA samples were prepared from 1 μL total RNA per reaction using a reverse transcription kit (Takara Biotechnology, Tokyo, Japan) and stored at - 20°C for qRT-PCR assays ...
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bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcription (RT) was performed with 1.5 µg of total RNA and PrimeScript reverse transcriptase (Takara Bio Inc, Kusatsu, Japan). RT-quantitative (q)-PCR was performed on a CFX96 C1000 real-time PCR cycler (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa, Japan) with specific primers (Table S2) ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of total RNA per sample was used to generate first-strand cDNA using reverse transcription system (Takara, Dalian, China). Quantitative RT-PCR was performed using SYBR Premix Ex Taq (Takara ...
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bioRxiv - Biochemistry 2020Quote: ... The target 23-mer sequences for sgRNA used were 5’-TTTACCTTGATAGGTGGTAGTGG - 3’and 5’-ATAAAGAGAGGCTTCTCGGGAGG - 3’ and synthesized using Guide-it™ sgRNA In Vitro Transcription Kit (TaKaRa) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) on a Thermal Cycler Dice Real Time System TP800 (Takara Bio) ...
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bioRxiv - Neuroscience 2021Quote: ... The single strand cDNA for 5’RACE was prepared by in vitro reverse transcription with avian myeloblastosis virus reverse transcriptase XL (Takara Bio) using total RNA (0.5 µg ...
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bioRxiv - Plant Biology 2021Quote: ... followed by reverse transcription using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Shiga, Japan). Quantitative PCR was conducted using the KAPA SYBR FAST qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA were also extracted from in vitro samples of cultured GC and reverse transcription reactions were performed using the SMART PCR cDNA synthesis technology (Takara Bio.) according to the manufacturer’s procedure and as previously published 35 ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was then subjected to genomic DNA (gDNA) elimination and reverse-transcription according to PrimeScript™ RT reagent kit protocols (Takara). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... . cDNA was synthesized by the use of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by the use of PrimeScript™RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2022Quote: ... Total RNA from SARS-CoV-2 infected lung or trachea was subjected to one-step real-time reverse transcription PCR using One-step PrimeScript RT-PCR kit (Takara, RR064B). Multiplex PCR was performed to detect SARS-CoV-2 nucleocapsid gene and mouse Actb gene ...
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bioRxiv - Cell Biology 2019Quote: ... from each ample was used to synthesize first-strand cDNA by reverse transcription using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... the in vitro Cas9 cleavage was performed using a previously amplified target pyrG amplicon following the steps of the Guide-it™ sgRNA In Vitro Transcription and Screening Systems User Manual (Takara Bio USA ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and reverse transcription and cDNA amplification were performed using 10 ng of RNA using the SMARTer Ultra Low RNA kit (Clontech Laboratories) like previously reported 13 ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid vectors were linearized to generate templates for viral RNA in vitro transcription (T7 RNA Polymerase System, Takara Bio, Shiga, Japan). RNA1 to RNA3 were equally mixed to infect 3-week-old N ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription and cDNA library preparation were performed with a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, 634888). cDNA was fragmented with a Covaris S 220 instrument (Covaris) ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Cell Biology 2023Quote: was used to carry out reverse transcription (RT) reaction to obtain cDNA following the instruction of PrimeScript RT Reagent Kit (Takara, Japan). RT primers are specific to midRs ...
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bioRxiv - Neuroscience 2023Quote: ... Two micrograms of the RNA were subjected to reverse transcription with random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). For semi-quantitative PCR ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Genomics 2023Quote: ... The same amount of input RNA for each batch was used for reverse transcription (RT) reactions with SMARTScribe™ Reverse Transcriptase (Takara) and oligo dT following the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on 1 µg total mRNA per sample using PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA removal and reverse transcription were carried out using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio).
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bioRxiv - Microbiology 2020Quote: ... The transgenes were inserted between the P and M gene of pNDV LaSota (LS) wild type or the L289A (15, 22, 23) mutant (NDV_LS/L289A) antigenomic cDNA by in-Fusion cloning (Clontech). The recombination products were transformed into NEB® Stable Competent E ...
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bioRxiv - Microbiology 2020Quote: ... The HSP4 amplicon was purified and 75ng incubated with 50ng of EcoRV digested puc19_RV vector for 15 minutes at 50°C and transformed into Stellar competent cells (TAKARA). Positive clones were verified by Sanger sequencing ...