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Citations for Takara Bio :
251 - 300 of 791 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The qPCR was performed using SYBR Green qPCR Master Mix (Takara ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Immunology 2023Quote: ... followed by RNA reverse transcription by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). After enzymatic fragmentation of cDNA samples by KAPA Frag kit (KAPA Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... from which cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (TaKaRa, Kusatsu, Japan). Transcript levels of RvNaRL were examined using a real-time PCR system (LightCycler 96 system ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of RNA was carried out using the PrimeScript RT reagent Kit with gDNAEraser from TAKARA. The resulting cDNA from reverse transcription was then amplified by PCR and digested using PstI ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by SYBR Green PCR Master Mix (Takara). The primer sequences for qRT-PCR were listed in Table S15.
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bioRxiv - Biophysics 2020Quote: ... the lysate was pumped through a column containing 15 mL of Talon Cobalt resin (TAKARA). The resin was washed without using imidazole using a wash cycle consisting of low salt (20 mmol/L Tris Base ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 μL of ligated reactant (15 ng DNA) was amplified using TaKaRa Ex-Taq (TaKaRa) with the following conditions ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted solution was concentrated using an Amicon Ultra-15 centrifugal filter unit (Takara bio). The titre was measured via real-time PCR using an AAVpro Titration Kit for Real Time PCR Ver ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified by 15 cycles of PCR using the Advantage 2 PCR Kit (Clontech) with template switching custom oligo (TableS6) ...
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bioRxiv - Immunology 2021Quote: ... and quantitative reverse transcription-polymerase china reaction (RT-qPCR) was performed using SYBR Premix Ex Taq (TaKaRa, China). All procedures were performed following the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized by reverse transcription using PrimeScript RT reagent kits with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA was DNAse I-treated (ThermoFischer Scientific) and submitted to reverse transcription (Takara PrimeScript™ RT). Before qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Three μg of total RNA was subjected to reverse transcription using PrimeScript 1st strand cDNA Synthesis Kit (Takara).
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bioRxiv - Neuroscience 2020Quote: ... reverse transcription and amplification was achieved using the SMART-Seq v4 ultra low input RNA Kit (Takara, 634891). This kit improves synthesis of the full-length cDNA via a template switching mechanism for synthesis of the second strand cDNA ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was done using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, #RR047A). qPCR reaction was prepared by TB Green® Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized by reverse transcription of 1 μg RNA using PrimeScrip RT Master Mix(Takara). Quantitative real time-PCR was performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription reactions containing 1 μg of total RNA were performed using PrimeScript™ (Takara, Otsu, Shiga, Japan). Individual cDNAs were amplified with THUNDERBIRD™ SYBR qPCR Mix (TOYOBO CO ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse transcription was performed to generate cDNA using the PrimeScript RT Master Mix (RR036; Takara, Kusatsu, Japan). The RNase-Free DNase Set (79254 ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Immunology 2020Quote: ... The reverse transcription of RNA into cDNA was performed using a Prime Script TM RT Master Mix (Takara). The following was the reaction system ...
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bioRxiv - Microbiology 2021Quote: ... Real-time reverse transcription-PCR was performed using a Thermal Cycler Dice real-time system MRQ (Takara Bio) with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was first amplified by reverse transcription using the PrimeSCript RT reagent kit (Takara Bio, Inc, Otsu, Japan). Then the relative expressions were measured with TaqMan MicroRNA Assay kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using a PrimeScript™ RT Reagent Kit (Takara Bio, Mountain View, CA 94043 USA). qPCR was performed with gene specific primers and SYBR Green (iTaq Universal SYBR Green Supermix ...
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bioRxiv - Plant Biology 2021Quote: ... one microgram of DNase-treated RNA was used for reverse transcription using a PrimerScript RT reagent kit (Takara) and a mix of oligo poli-dT and random hexamers ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 ng of RNA was used for the reverse transcription reaction using SMART-seq HT (Takara, 634455). For the PDO xenograft ...
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bioRxiv - Pathology 2023Quote: ... Reverse RNA transcription was performed using the PrimeScript RT Reagent Kit with gDNA Eraser (RR047A; Takara Bio, Japan). A 10 μL reaction mix was formulated as follows ...
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bioRxiv - Cell Biology 2022Quote: ... and in-chip reverse transcription PCR were performed using a 3’ DE Chip and Reagent kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... after which first-strand cDNA was synthesized from total RNA using a cDNA reverse transcription kit (TaKaRa, Japan). Full-length CDS sequences of the three candidate UGT genes were obtained from third-generation transcriptome sequencing data (Chen et al ...
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bioRxiv - Immunology 2023Quote: Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). Barcoded Illumina-compatible libraries were generated from 5 to 10Lng of DNA of each sample ...
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bioRxiv - Physiology 2023Quote: ... Reverse transcription (RT)-PCR was performed using the Emerald Amp MAX PCR master mix (Takara Bio, Shiga, Japan). Quantitative RT-PCR was performed using THUNDERBIRD SYBR qPCR MIX (TOYOBO Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
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bioRxiv - Neuroscience 2021Quote: ... 72°C for 15 s performed using a Takara Thermal Dice Real-time system III (Takara) or a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA was reverse-transcribed to single-stranded cDNA using the AMV Reverse Transcription System (Takara, Dalian, Liaoning, China). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal volume RNAs were subjected to reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...
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bioRxiv - Neuroscience 2021Quote: Reverse transcription (RT) was performed using a PrimeScript® RT Reagent Kit with gDNA Eraser (RR047A, TaKaRa, Dalian, China) in a 20 μL reaction mixture containing 1 μg of total RNA from each individual sample ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was carried out with 1 μg RNA using TB Green Premix Ex Taq™ II (Takara, Japan). Targets of around 110 bp were amplified with primers qtaxB-F/R ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNAs were prepared following the instruction (Guide-it™ sgRNA In Vitro Transcription and Screening Systems, TaKaRa, Japan). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR) (Takara Cat no.RR820A) to reflect the replication efficiency of the virus ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was then initiated with the cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit; Takara) using the random hexamers option ...
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bioRxiv - Genetics 2022Quote: ... 1 µg of total RNA was used for reverse transcription using PrimeScript RT Reagent Kit (Cat#RR037A, TaKaRa, Japan). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Genomics 2021Quote: Total RNA extracted from cultured cells was used for reverse transcription using the PrimeScript RTase (Takara Bio, Shiga, Japan) with random hexamers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PCR products were cloned into the transcription vector pTB-207 [88] using the In-Fusion HD Cloning kit (Clontech) as described previously [89] ...