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Citations for Takara Bio :
351 - 400 of 2175 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... After 4 h total RNA was extracted using RNAiso Plus (Takara Bio, Shiga, Japan) according to the manufacturer’s protocol and used for real-time quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was applied to 4-mL Talon Metal Affinity Resin (Clontech, cat# 635503). After washing with 30 mL wash buffer containing 20 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... the larvae were fixed in 4% PFA for imaging or RNAiso Plus (Takara, 9109) for RNA isolation.
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bioRxiv - Biophysics 2021Quote: ... The resultant supernatant was mixed with 4 mg of Talon Metal Affinity Resin (Takara) equilibrated with wash buffer [50 mM Na-Pi pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 and StNPR3/4 were inserted also into the pGADT7 (prey) vector (Clontech, USA), to produce proteins with an N-terminal Gal4 activation domain ...
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bioRxiv - Genomics 2024Quote: Approximately 4×106 HAP1 cells were transfected using Xfect Transfection Reagent (#631318, Takara Bio) for each pSpCas9(BB)-2A-Puro construct ...
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bioRxiv - Pathology 2023Quote: ... Affinity purification was performed at 4°C using Talon metal affinity resins (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Bioengineering 2024Quote: ... Cas9-EDVs were produced by seeding approximately 4 million Lenti-X cells (Takara Bio) into 10 cm tissue culture dishes (Corning ...
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bioRxiv - Genetics 2021Quote: ... The clarified lysate was subjected to Talon Metal Affinity column (Takara, Mountain View, CA) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the Takara LA HS Taq long range polymerase (Takara, Mountain View, CA, USA). PCR conditions used were 94°C for 3 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... transfected at 5-7 DIV with the plasmid peGFP-N1 (Clontech, Mountain View, CA) using lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara Bio, Mountain View, CA, USA). CasRx-GFP lentiviral particles were produced by co-transfecting plasmids encoding CasRx-GFP (PXR001 ...
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bioRxiv - Molecular Biology 2021Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara Bio, Mountain View, CA, USA). To obtain cells with stable CasRx-GFP expression ...
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bioRxiv - Neuroscience 2021Quote: ... according to the manufacturer’s user manual (Takara Bio USA, Inc. Mountain View, CA, USA). One hundred pg of amplified cDNA was tagmented and indexed for sequencing using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Chip wells were sealed using SmartChip Optical Imaging Film (Takara Bio USA, CA, USA) and centrifuged at 300 X g for 5 min at 22 °C ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... gel purified and cloned into a pEGFP-C1 vector (Clontech, Mountain View, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... 15 nucleotide overlaps were included in primers for infusion cloning (Clontech, Mountain View, CA). Where this failed ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cDNA amplification using the Clontech SMARTer kit (Takara Bio, Mountain View, CA).
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bioRxiv - Plant Biology 2021Quote: All yeast protocols were performed according to the Yeast Protocols Handbook (Clontech, Mountainview, CA) with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were tagged with Gfp (enhanced green fluorescent protein; Clontech, Mountain View, CA, USA) at their N-terminus unless noted otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were immunolabeled with antibodies against GFP (Clontech Laboratories Inc, Mountain View, CA) at 1:25 diluted in PBS/1% fish skin gelatin ...
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bioRxiv - Cell Biology 2020Quote: ... into the ICELL8® 350v Chip (640019, Takara Bio USA, Mountain View, CA, USA). We used the mixed buffer of PBS and fiducial mix (640202 ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The PCR product was ligated to pEGFP-C1 vector (Clontech Laboratories, Mountain View, CA), resulting in plasmid pEGFP-hUFL1 ...
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bioRxiv - Cell Biology 2021Quote: ... or the pZsGreen vector (cat. # 632446; Takara Bio USA, Inc., Mountain View, CA, USA). Transformed E ...
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bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequence of TgAP3β was cloned into pGADT7 (Clontech Laboratories, Mountain View, CA) and co-transformed into competent AH109 yeast cells with a plasmid encoding E ...
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bioRxiv - Plant Biology 2021Quote: ... followed by purification via His60 Ni Gravity Column Purification Kit (Clontech Laboratories, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Lentiviruses were packaged in Lenti-X 293T cells (cat#632180, Clontech, Mountain View, CA) using a three-plasmid expression system (pLKO.1-shRNAs-puro ...
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bioRxiv - Physiology 2021Quote: Plasmid DNA encoding enhanced green fluorescent (EGFP)-actin (Takara Bio USA, Mountain View, CA) facilitated exogenous gene expression in rodent kidneys ...
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bioRxiv - Immunology 2020Quote: All cloning was performed using In-Fusion cloning (Takara Biosciences, Mountain View, CA USA). Insert sequences were purchased from Sigma-Aldrich or Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2021Quote: ... we used the SMART-Seq v4 kit (Takara Bio USA, Mountain View, CA, USA) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification was achieved using TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA). This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega ...
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bioRxiv - Immunology 2020Quote: ... 20 μl of supernatant was titered using Lenti-X GoStix (Clontech, Mountain View, CA) per the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was incubated with equilibrated TALON Metal Affinity Resin (Takara Bio USA, Inc., CA) for 20 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... Cloning was conducted using an In-Fusion HD cloning kit (Takara Bio, CA, USA), and the resulting plasmids were named pTVT7_YG1_S ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR with the SeqAmp PCR kit (Takara Bio USA, San Diego, CA). Unlike TARGET-Seq ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45µm filter and concentrated with RetroX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45um filter and concentrated with LentiX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... CD34+ cells were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVO 10 media (Lonza ...
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bioRxiv - Pathology 2023Quote: Yeast transformation was performed according to the manufacturer’s instructions (Clontech, Mountain View, CA, USA). Yeast cells (strain Y2H Gold ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T cell line (Lenti-X™ 293T, Takara Bio, San Jose, CA, USA) was cultured in DMEM High Glucose (DMEM-HG cat# SH30243.02 ...
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bioRxiv - Immunology 2023Quote: HA-tagged TRIM34ΔSPRY constructs were cloned in pQCXIP (TaKaRa Bio, San Jose, CA, #631516) by SbfI (New England BioLabs #R3642S ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and were applied to the SMART RACE kit (Takara Bio USA, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: All yeast work was performed according to the yeast protocols handbook (Clontech, Mountainview, CA). For Y2H screening ...
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bioRxiv - Systems Biology 2024Quote: ... Mir-X miRNA qRT-PCR TB Green kit (Takara BIO, San Jose, CA, USA) was used for cDNA synthesis and miRNA expression analysis ...
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bioRxiv - Microbiology 2024Quote: Tissue samples were collected and total RNA was extracted using Trizol (Takara, CA, JPN) as previously described ...
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bioRxiv - Developmental Biology 2024Quote: ... Seventy-percent confluent Lenti-XTM 293T cells (Takara Bio USA, Inc., Mountain View, CA) were transfected with Hdac3 lentiviral plasmids (Hdac3 KO or empty vector control [EV])26 ...
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bioRxiv - Molecular Biology 2024Quote: ... and a vesicular stomatitis virus glycoprotein (VSV-G)-expressing plasmid (Clontech, Palo Alto, CA), pVSV-G [43] ...
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bioRxiv - Cell Biology 2024Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with pCMV-Tet3G plasmid (631335, Takara Bio USA, San Jose, CA) and either of TRE-Empty ...