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Citations for Takara Bio :
151 - 200 of 2175 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... or pGADT7 (AD) vector (Clontech, Mountain View, CA), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry-C1 plasmid (Clontech, Mountain View, CA, USA) at 1 ng/μl ...
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bioRxiv - Microbiology 2024Quote: Escherichia coli Stellar cells (TaKaRa, Mountain View, CA) were used for routine cloning and isolation of plasmid DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... GP2-293 cells (Takara Bio, San Jose, CA) and 293FT cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... pEGFP-N1 (Takara Bio USA, Inc., CA, USA) was linearized with homologous arms by inverse PCR using primers 1 and 2 listed in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight primary antibody incubation was done at 4°C with one or more of the following antibodies in blocking buffer: rabbit anti-DsRed (Takara Bio, 632496, 1:500), goat anti-mCherry (Sicgen ...
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bioRxiv - Plant Biology 2021Quote: ... 4 U and 8 U of MNase (TaKaRa 2910A) in MNase digestion buffer containing 20 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of lung tissue RNA (Takara Bio, 636524) at a final concentration of 500 pg per μl was used ...
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bioRxiv - Immunology 2024Quote: ... and RNAse inhibitor (4 units) (Takara Bio, London, UK). After each plate was filled ...
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bioRxiv - Biochemistry 2024Quote: ... 4 ml TALON cobalt resin slurry (Takara Bio Inc.) was added (1 ml/tube ...
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bioRxiv - Neuroscience 2021Quote: ... floating coronal sections were rinsed in PBS and blocked for 1–2 hr at room temperature in a solution of 10% normal goat serum and 0.5% Triton X-100 dissolved in PBS and then incubated in blocking solution containing rabbit anti-DsRed polyclonal antibody (1:1000; Takara Bio, Mountain View, CA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Microbiology 2021Quote: ... The knockout construct was developed through In-Fusion using the purified amplicons based on the manufacturer’s instructions with the insert to vector ratio of 2:1 (Takara Bio, Mountain View, CA, USA) and transformed into E ...
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bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
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bioRxiv - Immunology 2020Quote: cDNAs encoding EqPD-1 and EqPD-L1 were amplified by PCR using gene-specific primers with restriction enzyme cleavage sites (Supplementary Table 1) and subcloned into the multicloning site of pEGFP-N2 (Clontech, Palo Alto, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: Human c-Jun sequence was amplificated using the cDNA of MCF7-BM02-1 and then transferred to pMXd3-PEF1-IRES-Puro vector using an In-Fusion Advantage PCR Cloning Kit (Clontech Laboratories Inc., CA, USA). The sequence encoding the transactivation domain of c-Jun in pMXs-Jun-IH was kindly provided by Dr ...
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bioRxiv - Cell Biology 2024Quote: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r ...
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bioRxiv - Bioengineering 2023Quote: ... Protease inhibitors and 10% glycerol were added to the supernatant before loading it onto 1 mL of Talon metal affinity resin (Clontech, Mountain View, CA, #635502). After a 20 mL buffer wash ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with pGAL4-BD-Nmono using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA ...
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP or pC1-tdTomato (Clontech; Mountain View, CA) were mixed with AMPAR-expressing plasmids at a base pair ratio of 1:7 ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with this construct using a standard lithium-acetate protocol ...
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bioRxiv - Microbiology 2021Quote: ... AH109 yeast cells (Clontech; Takara, Mountain View, CA, USA) were transformed with this construct using a standard lithium-acetate protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and 10% FBS (Clontech Laboratories, Mountain View, CA, USA). All cell lines (LN229 (Accession Nr ...
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bioRxiv - Cell Biology 2020Quote: ... the pRetro-X vector system (Clontech, Mountainview, CA, USA) was used ...
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bioRxiv - Microbiology 2020Quote: ... Using In-fusion HD cloning (Clontech, Mountain View, CA), the resulting fragment was cloned into pMSP3535 (50) ...
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bioRxiv - Microbiology 2020Quote: ... Using In-fusion HD cloning (Clontech, Mountain View, CA), the resulting fragment was cloned into pMGS100 (49) ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for Western blotting at a dilution of 1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... Talon cobalt resin was from Clontech (Mountain View, CA). All lipids were purchased from Avanti Polar lipids (Alabaster ...
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bioRxiv - Cell Biology 2021Quote: ... following manufacturer’s protocol (Takara Bio, Mountain View, CA, USA). Lentivirus vectors pLVX-Tet3G and pLVX-TRE3G (Cat ...
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bioRxiv - Biochemistry 2022Quote: ... His60 Ni Superflow Resin from Takara (Mountain View, CA); 5mL GSTrap Fast Flow column from Cytiva (Marlborough ...
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bioRxiv - Bioengineering 2022Quote: ... concentrated with Lenti-X concentrator (Clontech, Mountain View, CA), and stored at −80C until use.
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bioRxiv - Synthetic Biology 2022Quote: ... coli Stellar cells (Clontech Laboratories, Inc., Mountain View, CA) and PCR identified clones were confirmed by DNA sequencing.
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bioRxiv - Plant Biology 2023Quote: ... were amplified and cloned into pAbAi (Clontech, CA, USA) to generate the bait vectors (Pro-SAL1-AbAi and Pro-NHX1-AbAi) ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-Myc-N (635689; Clontech, Mountain View, CA, USA), and pEGFP-C3 (#6082-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The adherent cells HEK293T-LentiX (TAKARA, Mountain View, CA) were grown in DMEM (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Mighty Mix (Takara Bio USA, San Jose, CA, USA), and transformed into chemically competent TOP10 E.coli cells (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli Stellar (TaKaRa Bio USA, Mountain View, CA) was used for all cloning ...
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bioRxiv - Developmental Biology 2024Quote: ... and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The supernatant of the sonicated cells was incubated with equilibrated TALON Metal Affinity Resin (Takara Bio USA ...
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bioRxiv - Neuroscience 2020Quote: ... with a total of 1 µg of good quality total RNA as input and enriched using the SMARTer Apollo NGS library prep system (Takara Bio USA, Mountain View, CA). Sequencing libraries were prepared using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs that were used for RACE were synthesised from 1 µg of polyA+ RNA using a SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T or UFSP2 KO HEK293T cells (16) were transfected with the aforementioned pX330 vectors together with pEGFP-C1 (#6084-1, Clontech Laboratories, Mountain View, CA, USA) and cultured for 2 d ...
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bioRxiv - Physiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using RNA to cDNA EcoDry™ Premix (Double Primed) (Takara Bio USA Inc., San Jose, CA, USA) according to the manufacturer instruction ...
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bioRxiv - Physiology 2024Quote: Libraries were prepared by the Van Andel Genomics Core (Grand Rapids, MI) from 1 ng of total RNA using Takara SMART-Seq Stranded Kit (Takara Bio USA, Mountain View, CA) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNAs of these MOP variants were then PCR-amplified from the pJET2.1 plasmids and inserted into the BamHI site of the pTRE2hyg mammalian expression vector (Clontech, Palo Alto, CA, USA, #6255-1) using In-Fusion cloning (Takara Bio ...
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bioRxiv - Biochemistry 2024Quote: ... To the resulting solubilized membranes was added 1 mM imidazole (final conc.) and this was combined with 0.5 mL settled TALON resin (Takara Bio USA, Inc., San Jose, CA) that had been pre-washed with homogenization buffer ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated at 4 °C in Lenti-X Concentrator (Clontech) for 1 h ...
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bioRxiv - Genomics 2023Quote: ... 4 µl of Dr.GenTLE precipitation carrier (Takara, cat. no. 9094) and 4 µl sodium acetate ...
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bioRxiv - Microbiology 2021Quote: ... We PCR amplified each region from 1 μL of the converted gDNA using TaKaRa EpiTaq HS for bisulfite-treated DNA (Takara Bio USA; Mountain View, CA, USA) per manufacturer’s instructions with primers designed using MethPrimer ...
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bioRxiv - Genetics 2021Quote: ... Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries ...