Labshake search
Citations for Takara Bio :
551 - 600 of 2175 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and hSTEAP2 was purified from the supernatant using cobalt-based Talon affinity resin (Clontech, Mountain View, CA). The C-terminal His6-tag was cleaved with tobacco etch virus (TEV ...
-
bioRxiv - Neuroscience 2023Quote: Fhod3 cDNA was amplified with PrimeSTAR® GXL DNA Polymerase (R050B; Takara Bio, San Jose, CA, USA) using a pair of primers (5’-CACCATGGCCACGCTGGCTTGTCGCGTGCAG3’ for the forward and 5’-GAGCATGCTCACAGTTGCAGTTCAGATGTG −3’ for the reverse primer) ...
-
bioRxiv - Molecular Biology 2024Quote: ... while amplicons over 5 kb were produced using PrimeStar GXL polymerase (Takara Bio, San Jose, CA, #R050A) in a two-step amplification reaction ...
-
bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
-
bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The selected yeast strains were seeded in SD medium with Dropout (DO) supplement-His (Clontech, CA, US) on a sterile black clear-bottom 384-well plate (Corning ...
-
bioRxiv - Biophysics 2024Quote: ... Cells were harvested and purified on Talon His-Tag Purification Resin (Takara Bio, Mountain View, CA, USA). The hexahistidine tag was removed by incubating overnight at 4°C with TEV protease ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4°C with an anti-GFP monoclonal primary (JL-8; Clontech 632381) at 1:5,000 dilution ...
-
bioRxiv - Plant Biology 2022Quote: ... and His (-HTLA) but containing 5-bromo-4-chloro-3-indolyl α-D-galactopyranoside (Clontech, Madison, WI, USA). As a control ...
-
bioRxiv - Microbiology 2021Quote: ... and 10 ng of Renilla luciferase (pTK r.luc) by using 4 µl of TransIT 293 (Takara, Shiga, Japan) and 100 µl of OPTI- MEM (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... secreted protein was purified from the culture supernatant 4 days post transfection using TALON metal affinity resin (Clontech) and Amicon Ultra 10K filter device (Millipore) ...
-
bioRxiv - Genomics 2020Quote: Lysis plates were prepared by dispensing 0.3μL lysis buffer (4 U Recombinant RNase Inhibitor (RRI) (Takara Bio, 2313B), 0.12% Triton™ X-100 (Sigma ...
-
bioRxiv - Plant Biology 2022Quote: ... and histidine (H) and containing 5-Bromo-4-Chloro-3-Indolyl α-D-galactopyranoside (X-α-gal) (Clontech) to detect interactions ...
-
bioRxiv - Plant Biology 2023Quote: ... Ade and His but containing 5-Bromo-4-Chloro-3-indolyl a-D-galactopyranoside (X-a-gal) (Clontech). To detect interactions ...
-
bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio ...
-
bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (1:200-1:500, Clontech), mouse anti-1D4 anti-Fasciclin II (1:10 ...
-
bioRxiv - Molecular Biology 2024Quote: ... doxycycline (DOX, 1 µg ml−1, Takara Bio) and 2% penicillin–streptomycin (Gibco) ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 AtCBLs prey vectors were co-transformed into the yeast strain AH109 following manufacturer’s instructions (Clontech, CA, USA). The empty vectors pTOOL27 and pTOOL28 were also co-transformed into yeast with each prey or bait plasmid respectively to confirm that bait does not autonomously activate the reporter genes in the absence of the prey protein.
-
bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
-
bioRxiv - Genomics 2020Quote: ... Images (TIFF format) were analyzed using automated microscopy image analysis software Cell Select (Takara Bio USA, CA, USA). The chip was stored in a chip holder at −80 °C overnight ...
-
bioRxiv - Genomics 2021Quote: ... according to the technical manual for PrimeScriptTM First Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript ...
-
bioRxiv - Biochemistry 2021Quote: ... was used to remove two SapI restriction sites from vector pEGFP-C1 (Takara Bio USA, Mountain View, CA). A Woodchuck hepatitis virus (WHP ...
-
bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
-
bioRxiv - Systems Biology 2021Quote: ... Ribosomal RNA was depleted using the RiboGone – Mammalian Kit protocol (Cat #634847, Takara Bio USA, Mountain View, CA). The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938 ...
-
bioRxiv - Cell Biology 2020Quote: ... Counted cells were dispensed into microwells using the ICELL8 MSND (640000, Takara Bio USA, Mountain View, CA, USA) at the concentration of 25 cells/μl in 0.5× PBS and 1× Second Diluent (640202 ...
-
bioRxiv - Cell Biology 2020Quote: ... We used the mixed buffer of PBS and fiducial mix (640202, Takara Bio USA, Mountain View, CA, USA) as the negative control wells ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA isolation and reverse transcription were performed as described in the STAR METHODS section “Ddi2 expression analysis in mouse embryos using qRT-PCR.” Constructs encoding the DDI2WT and DDI2PD proteins were cloned into p905 (gift from Pavlína Řezáčová, IOCB CAS, Prague) and pTreTight (Clontech) expression vectors.
-
Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... HSV-tkSR39-T2A-sIL2 construct was cloned from HSV-tkSR39-GFP using In-Fusion cloning (Takara Bio, CA) after adding six C-terminal amino acids (EMGEAN ...
-
bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
-
bioRxiv - Plant Biology 2021Quote: Plasmids for Citrine or mNeon-Green endogenous tagging were assembled using In-Fusion (Clontech, Mountain View, CA, USA), in which Citrine or mNeonGreen genes ...
-
bioRxiv - Neuroscience 2021Quote: ... filtered through Whatman 0.45 µm CA filter (GE #10462100) and concentrated using Lenti-X™ Concentrator (Takara #631232) according to producer datasheet (overnight incubation) ...
-
X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... showing a viral titer of >106 IFU/ml as measured by Lenti-X GoStix (Takara, Mountain View, CA). To develop stably expressing OptoXR-EYFP cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
-
bioRxiv - Cell Biology 2022Quote: ... Titer of the lentiviruses was measured with a lentivirus titration kit (Takara Bio USA, San Jose, CA, USA). The same multiplicity of infection (MOI ...
-
bioRxiv - Cell Biology 2022Quote: ... which was used to screen a Mate & Plate™ Library-Universal Mouse (Normalized) (Clontech, Mountainview, CA; Cat#: 630482) using the stringent protocol according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cDNA sequence of ERK5 isoforms were cloned into pLVX-Puro plasmid (Clontech Laboratories, Mountain View, CA, USA). Plasmids were sequenced to confirm the presence of correct cDNA sequences at the 3’-end of the CMV promoter (data not shown) ...
-
bioRxiv - Genomics 2021Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountain View CA).
-
bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using a PrimeScript™ RT Reagent Kit (Takara Bio, Mountain View, CA 94043 USA). qPCR was performed with gene specific primers and SYBR Green (iTaq Universal SYBR Green Supermix ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Both missing ends could be recovered by RACE PCR (SMARTer RACE kit, Takara Bio, Mountain View, CA, USA).
-
bioRxiv - Neuroscience 2021Quote: ... The transfection efficiency of HEK293 cells was evaluated by transfecting cells with EGFP-N1 (Clontech; Mountain View, CA) in parallel reactions ...
-
bioRxiv - Plant Biology 2022Quote: ... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
-
bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs were synthesized using the SMARTer RACE cDNA Amplification Kit (Cat. No. 634858; Takara, Mountain View, CA, USA). In silico predicted pou-iv gene sequence was retrieved from the Joint Genome Institute genome database (Nematostella vectensis v1.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products then were inserted into a Tet-on inducible lentiviral vector pLVX (Takara, Mountain View, CA) through EcoRI and BamHI restriction sites ...
-
bioRxiv - Immunology 2021Quote: ... Full-length cDNA was generated using the Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) prior to library generation with the Nextera XT DNA Library Prep kit (Illumina ...
-
bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
-
A bacterial derived plant- mimicking cytokinin hormone regulates social behaviour in a rice pathogenbioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA was reverse transcribed into cDNA using EcoDryTM Premix (Clontech, Mountain View, CA, USA) according to the manufacturer’s instructions using random hexamer primers ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were prepared using SMARTer kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera protocols (Illumina ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cloning was carried out using the ligation-independent In-Fusion® HD cloning kit (Takara Bio USA, CA). Lentivirus production and transduction were done as described 33 ...