Labshake search
Citations for Takara Bio :
451 - 500 of 2175 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... siRNA SMARTpool was mixed with pEGFP-N1 plasmid obtained from Clontech (Mountain View, CA, USA) at a final concentration of 50nM ...
-
bioRxiv - Plant Biology 2024Quote: ... which was performed using a SMARTer RACE 5’/3’ Kit (Clontech Laboratories, Mountain View, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Titer was measured using the Adeno-X Rapid Titer Kit (Takara Bio, Mountain View, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
-
bioRxiv - Neuroscience 2022Quote: ... we carry out all steps at 4°C and in the presence of RNase inhibitor (Takara). We use the isolated nuclei for the droplet-based 10x scRNA-seq assay ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.3 μl of lysis buffer (0.13% Triton-X-100, 4 units of recombinant RNase Inhibitor, Takara) was added to 3.7 μl of cell-free CSF and plasma ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3.5 μL nuclease-free water was added to 4 μL of 5x PrimeScript buffer (Takara, USA), 1 μL RNAse OUT (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... and the supernatant was incubated overnight at 4 °C with Co2+-charged affinity resin (Talon, Clontech) and 30 mM imidazole.
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µM Shield-1 (Takara # 632189) for 4 hours ...
-
bioRxiv - Genetics 2022Quote: ... 1 μgml−1 doxcycline (TAKARA Bio) and 10−6 M dexamethasone (Sigma Aldrich).
-
bioRxiv - Biochemistry 2020Quote: ... Lentivirus particles were generated using the Lenti-X Single Shot system (ClonTech, Mountain View, CA, US) in 293T-Lenti-X cells ...
-
bioRxiv - Neuroscience 2021Quote: ... Neuronal morphology of 14 DIV neurons transfected with the plasmid peGFP-N1 (Clontech, Mountain View, CA) was examined using high-resolution confocal digital images obtained from an Opera-Phenix microscope (Perkin-Elmer) ...
-
bioRxiv - Genomics 2020Quote: ... 250 nL volume ICELL8 chip (SMARTer™ ICELL8® 250v Chip, Takara Bio USA, CA, USA) using a Multi Sample Nano Dispenser (MSND ...
-
bioRxiv - Genomics 2020Quote: ... Each sample was PCR amplified for 9 cycles using HS LA Taq (Takara, Mountain View, CA) and cleaned with 0.7X AMPure beads to remove small fragments and excess reagents (Beckman Coulter ...
-
bioRxiv - Neuroscience 2020Quote: ... and then concentrated using the lenti-x concentrator (Cat. No. 631231, Clontech Laboratories, Mountain View, CA).
-
bioRxiv - Cell Biology 2021Quote: ... which was sub-cloned to a lentiviral pLVX-IRES-Puro vector (Takara Bio Inc, CA, USA) containing a puromycin resistance gene ...
-
bioRxiv - Cell Biology 2021Quote: ... ds-cDNA was made using Clontech SMARTer® Ultra Low RNA Kit (Clontech, Mountain View, CA) following manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2020Quote: ... The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio, CA, USA) on Bio-Rad CFX 96 C1000 with following conditions ...
-
bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq kit (TaKaRa Bio USA, San Jose, CA) and the CFX Connect real-time PCR machine (Bio-Rad ...
-
bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the Smart cDNA Library Construction kit from Clontech (Palo Alto, CA, USA) and normalized using the Trimmer cDNA Normalization kit from Evrogen (Moscow ...
-
bioRxiv - Molecular Biology 2021Quote: ... The experiment was conducted following the manufacturer’s instructions (Cat. No. 630489; Clontech, Mountain View, CA, USA).
-
bioRxiv - Neuroscience 2020Quote: ... PCR amplified and inserted into the DsRed-Express2 plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-eGFP with Halo tag (GenScript USA Inc ...
-
bioRxiv - Cell Biology 2021Quote: ... OCIAD1 was initially cloned from human cDNA into a pAcGFP-N1 vector (Clontech, Mountain View, CA). The GFP1-10 vectors were cloned by Gibson assembly into a FUGW lentiviral backbone (Addgene ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lentiviral particles were concentrated using the Clontech Lenti-X Concentrator (Takara Bio USA, Mountain View, CA). The concentrated lentivirus was used to transduce the cell lines ...
-
bioRxiv - Microbiology 2020Quote: ... Libraries were prepared with the SMARTer PrepX DNA Library Kit (Takara Bio, Mountain View, CA, USA), using the Apollo NGS library prep system (Takara Bio ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA XS kit (Clontech, Moutain View, CA). The Smarter Ultra low RNA input kit (Clontech ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: PCR reactions were performed using PrimeSTAR® Max DNA Polymerase (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... the cDNA encoding human Wt-syn was introduced into a vector from Clontech (Mountain View, CA) containing the mRFP sequence ...
-
Early life lipid overload in Native American myopathy is phenocopied by stac3 knock out in zebrafishbioRxiv - Genetics 2023Quote: cDNA (500-1000 ng) was synthesized using the Takara PrimeScript Kit (Takara, Mountain View, CA, USA). Gene expression was quantified using the PowerUp SYBR Green Master Mix and a ViiA™7 Dx qPCR Instrument (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were prepared using the SMART-Seq HT Kit (Takara Bio, Mountain View, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio, CA, USA) on Bio-Rad CFX 96 C1000 with following conditions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio, San Francisco, CA). cDNAs were then prepared by reverse transcriptase (Takara Bio ...
-
bioRxiv - Microbiology 2023Quote: ... The Rluc sequence was then replaced by the YFP sequence of pEYFP-N1 (Clontech, Mountainview, CA) by insertion into the NcoI-NotI sites ...
-
bioRxiv - Biophysics 2022Quote: ... The DNA shuffling reaction was carried out using PrimeSTAR HS DNA polymerase (Takara, Mountain View, CA) and pDS170 plasmids carrying the parent templates enNTS1ΔM10 (containing no methionine residues ...
-
bioRxiv - Zoology 2023Quote: ... including stranded library prep and treatment with Ribogone™ (Takara Bio USA Inc., Mountain View, CA) was performed by the Emory National Primate Research Center Genomics Core on the Illumina HiSeq 3000 system (single-end strand specific reads ...
-
bioRxiv - Microbiology 2022Quote: Protein-protein interactions were assayed with the Matchmaker yeast two-hybrid system (Clontech, Mountain View, CA). ORFs of AoHse was amplified from first-strand cDNA of A ...
-
bioRxiv - Biochemistry 2022Quote: ... Scaffolding protein was purified on a TALON Superflow metal affinity column (Takara Bio, San Jose, CA). Fractions containing scaffolding protein were pooled and concentrated in 12-14kDa MWCO dialysis tubing (Spectrum Chemical ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... cDNA was synthesized using a SMARTer RACE cDNA Amplification Kit (Clontech Laboratories, Mountain View, CA, USA). Gene-specific primers were designed using Primer3 Plus (Untergasser et al. ...
-
Gain-of-function study reveals the pleiotropic roles of serine protease HtrA in Borrelia burgdorferibioRxiv - Microbiology 2024Quote: ... 5′-RACE was carried out using SMARTer RACE 5’ Kit (Takara Bio USA, Mountain View, CA) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: RNA was converted into cDNA using the SMART-seq UltraLow v4 kit (Clontech, Mountain View, CA). Illumina cDNA sequencing libraries were prepared using the Nextera XT kit (Illumina ...
-
bioRxiv - Plant Biology 2024Quote: ... The Y1H assay was conducted using a Y1H Library Screening Kit (Clontech, Mountain View, CA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2024Quote: ... The Lenti-X p24 Rapid Titer kit was obtained from Clontech (Cat # 632200, Mountain View, CA). Antibodies for PKCδ ...
-
bioRxiv - Biochemistry 2024Quote: ... Plasmid DNA was purified using NucleoBond PC 2000 plasmid megaprep kits (Takara, San Jose, CA, USA). FreeStyle 293-F and Expi293 human embryonic kidney cells (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... Library was prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara bio USA, CA) with 7-cycle amplification.
-
bioRxiv - Immunology 2020Quote: ... Activated NK cells were transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
-
bioRxiv - Microbiology 2021Quote: ... and 20 ng of firefly luciferase (pGL) by using 4 µl of TransIT 293 (Takara, Shiga, Japan) and 140 µl of OPTI- MEM (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... RNA was then amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently ...
-
bioRxiv - Bioengineering 2020Quote: ... were mixed at a 1:1:1 ratio and bound to retronectin (Clontech)-coated plates according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100, Takara). The live singlet gated CD3+ T cells were further gated as per the gating strategy shown in additional file 1 ...
-
bioRxiv - Biochemistry 2020Quote: ... reverse P4 or P5 primers and PrimeStar GXL DNA polymerase (Takara Bio Inc., Mountain View, CA, USA). The resulted PCR product was cloned into TetR-DOZI plasmid49 by sequence- and ligation-independent cloning (SLIC ...