Labshake search
Citations for Takara Bio :
3801 - 3850 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... the synthesized cDNA products were subjected to real-time PCR in a reaction mixture (10 μl) containing Takara Premix Ex Taq (Probe qPCR) (TaKaRa, Kyoto, Japan), 200 nM hydrolysis probes and 200 nM PCR primers ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The amplified PCR products were digested with BamHI and HindIII and inserted into a EGFP-C1 vector (Clontech, Mountain View, CA, USA).
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... GFI1-BirA*-HA PCR products were cloned into the NotI and EcoRI sites of the pLVX-Tight-Puro vector (Clontech Laboratories, Inc). We used the rat GFI1 ortholog for our experiments ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were done on Roche LightCycler® 96 PCR system with the SYBR Premix Ex Taq™ Kit (TAKARA, RR420A). Gene expression levels were normalized to GAPDH and compared to gene expression levels in hESCs ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified using Dyne PCR Purification Kit (DyneBio, South Korea) and cloned into pPZP211 vectors using Takara T4 DNA Ligase (Takara Bio, Japan). Escherichia coli (DH5α ...
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bioRxiv - Microbiology 2022Quote: ... Mink ACE2 full length gene was amplified using long range PCR (LRPCR) amplification assay in two overlapping fragments using high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio USA) as previously described (34,35) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... served as template in a 20 μl qRT-PCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio Inc.) and the Roche LightCycler 96 system (Roche ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The RNA was eluted according to the protocol in 10 uL nuclease free water and equal amounts of RNAs from different samples were immediately subjected to amplification-based cDNA synthesis using the SMARTer PCR cDNA Synthesis Kit (Takara, 634925, 639207). We have standardized the amplification cycle to 15 for 2-10 ng of initial RNA template used for the reaction ...
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bioRxiv - Pathology 2022Quote: ... and expected amplicons of NS1 and VP1 genes were purified using Nucleospin® Gel and PCR clean-up kit (Takara Bio, Japan). Purified PCR amplicons were individually subjected to Barcode-Tagged sequencing (Celemics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Neuroscience 2019Quote: ... Real-time monitoring of the PCR amplification reaction was performed using an ABI 7300 fast real time PCR system (CA, USA) and SYBR®Premix Ex TaqTM (Tli RNaseH Plus) (TaKaRa, Japan). The primers used were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR mix was prepared using the plasmids obtained from TA cloning and CloneAmp HiFi PCR Master Mix according to manufactures recommendations (In-Fusion® HD Cloning Plus, Takara, 638910). This is followed by in-fusion reaction for site directed mutagenesis as we have done previously (Kocabas and Aslan ...
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bioRxiv - Cancer Biology 2019Quote: ... The open reading frame of AIRE was recovered by PCR using this plasmid as a template and PrimeSTAR GXL DNA Polymerase (Takara, Shiga, Japan). The PCR product was digested with EcoR1 and inserted into pcDNA6-3xFlagN (in-house plasmid made using pcDNA6/V5-HisA (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 3′ and 5′ rapid-amplification of cDNA ends Polymerase Chain Reaction (RACE-PCR) was carried out by using the SMART™ RACE cDNA Amplification Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: Each fragment was individually amplified in a 25-μL PCR reaction using 1 μL of PrimeSTAR GXL polymerase (Takara Bio Inc., #R050A), 1 μL of template DNA (10-100 ng μL−1 genomic DNA or plasmid DNA isolated from E ...
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bioRxiv - Zoology 2021Quote: ... and cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio Inc.). The amount of target RNA was normalized to an endogenous control ribosomal protein 49 gene (rp49) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelled amplicons were pooled and purified using NucleoSpin Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... The SLC7A1 cDNA from each sample was amplified by polymerase chain reaction (PCR) using PrimeSTAR GXL DNA polymerase (Takara Bio, Otsu, Japan). The amplification profile included 98 °C for 2 min followed by 30 cycles of 98 °C for 15 s ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time qPCR was performed in 48-well blocks with a StepOne Plus Real Time PCR System by using TaKaRa SYBR Premix Ex Taq II reagent kit (TaKaRa, Tokyo, Japan). All qRT-PCRs were performed in triplicate using total RNA samples extracted from three independent biological replicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The Rdl PCR-product was subcloned into the transcription vector pTB-207 38 using the In-Fusion® HD Cloning Kit (Clontech™) and cRNAs were synthesized with the T7 mMessage mMachine kit (Ambion™) ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... antisense PLANE and NCOR2 pre-mRNA were generated by PCR amplification from cDNAs using PrimerSTAR Max DNA Polymerase (TAKARA, #R045A; Dalian, China). Forward primers containing the T7 RNA polymerase promoter sequence and reverse primers without the promoter sequence were used for synthesizing PLANE ...
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bioRxiv - Biochemistry 2020Quote: ... Polymerase chain reaction (PCR) was used to amplify the intron 1 of HIF3A using EpiTaqTM HS (for bisulfite-treated DNA; Takara, Shiga, Japan). Levels of DNA methylation were quantified using pyrosequencing with the PyroMark Q24 Advanced kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and mNeonGreen were PCR-amplified and assembled into pPBbsr2 vector by using In-Fusion system (Takara Bio USA, Inc., Mountain View, CA). pCMV-mPBase (obtained from the Wellcome Trust Sanger Institute ...
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bioRxiv - Cell Biology 2021Quote: ... N’-Ter-CPE and the other truncated CPE constructs were constructed by amplifying CPE cDNAs by PCR and subcloning into pEGFP-N3 (Clontech, Oxon, UK) using XhoI and BamHI restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... and NOS3 were detected using quantitative real-time polymerase chain reaction (qRT-PCR) with TB Green™ Premix Ex Taq™ II (Takara) in the LightCycler96 system (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were cleaved using restriction enzymes and purified using electrophoresis and NucleoSpin Gel & PCR Clean-up kit (Takara bio, Shiga, Japan). The copy number of each standard DNA was calculated based on the concentration quantified with the Qubit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the RNAs of 150µl of the supernatant were extracted and quantified using Retro-X™ qRT-PCR Titration Kit (Takara, cat# 631453), according to the manufacturer instruction.
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bioRxiv - Microbiology 2022Quote: ... The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA). The EMSA binding reaction ...
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bioRxiv - Plant Biology 2022Quote: ... One base of 5’ dA overhang was added to the PCR amplicon of pMYB93 using Taq polymerase (Takara Bio Inc. Shiga, Japan), which was then cloned into pENTR5’-TOPO (Thermo Fischer Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragment containing the SHHc tag was combined with the digested backbone using In-Fusion HD cloning (Clontech, Mountain View, California) to make pK7-SHHc ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR assays were performed in final volumes of 25 μl containing 12.5 μl of 2 × PCR Taq Mastermix (MgCl, dNTP, Taq enzyme) (Takara Bio Inc., Japan); 0.5μl of each primer (10 mmol/L) ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Quantification of transcript was done by qRT-PCR with fluorescent-labelled nucleotide substrate (TB Green™ Premix Ex Taq™ II, Takara or PowerUp SYBR Green Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... and spectinomycin-resistant gene with lacI–spac promoter unit were amplified through polymerase chain reaction (PCR) using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan), along with appropriate primer sets (namely F1/R2 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in triplicates using TB Premix Ex Taq II (Takara Bio, #RR390A, Japan) in a QuantStudio 6 Flex Real-Time PCR System using QuantStudio Real-Time PCR Software (both Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
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bioRxiv - Cancer Biology 2024Quote: ... MYCN 5′ DEL and 3′ DEL deletion mutants were generated by restriction-free cloning using plasmid PCR amplification and overhang ligation using the In-Fusion Cloning Kit (Takara Bio 638910) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: The q-PCR standards were synthesized by cloning target genes into Escherichia coli DH5a using a PMD18-T vector Cloning Kit (TaKaRa Biotechnology, China). Vector concentrations were measured using NanoDrop ...
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bioRxiv - Synthetic Biology 2024Quote: ... To amplify the canvas and activity-encoding regions on the recorder plasmid construct,0 5µL of culture sample was directly used in a 10µL PCR reaction using PrimeStar Max master mix (Takara Bio, San Jose, CA) under conditions recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: The open reading frame (ORF) of Kaede was removed from pSPCiPC2Kaede by inverse PCR with PrimeStar GxL DNA polymerase (Takara-bio Inc). The ORFs of Gαq ...
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bioRxiv - Genomics 2024Quote: We applied three distinct cDNA library enrichment protocols for the RNA sample extracted from the same mice individual: i) standard PacBio Clontech SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.); ii ...
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bioRxiv - Bioengineering 2024Quote: ... the target scaffold sequence was first amplified in double-stranded DNA (dsDNA) form via PCR from M13mp18 using PrimeSTAR Max Premix (Takara Bio, R045A) and 0.8 µM primers (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... nine DNA fragments encoding the partial genome of SARS-CoV-2 (hCoV-19/Japan/TY-WK-521/2020) were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding the hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-time PCR system (TaKaRa Bio, Japan). The target genes included 27 ARGs and 5 MGEs (Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKara Bio, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were subcloned into EcoRV site of pBluescript SK- vector using In-Fusion® HD Cloning Kit (TaKaRa Bio, Japan) and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’ ...
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bioRxiv - Genomics 2023Quote: ... the human mtDNA control region (m.1-573 and m.16024-16569) was enriched using four overlapping PCR amplicons using high fidelity TaKaRa PrimeSTAR GXL DNA polymerase (TaKaRa; Table 2). PCR products were visually inspected by agarose gel ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated DNA was amplified by PCR using Hot Start TaKaRa LA Taq kit to yield a 10-Kb product (Takara Biotechnology, #RR042A). Primers utilized were the following ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The PCR products were used as templates to synthesize specific dsRNA using the in vitro Transcription T7 Kit (TaKaRa Biotechnology, Dalian, China) (dsRNA-Vitro ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 µl used as a source of genomic DNA in 20 µl PCR reactions with PrimeStar Hot Start DNA polymerase (Takara Bio, R010A). Amplified DNA was subcloned with StrataClone Blunt PCR cloning kit (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: The cDNA encoding human ZCCHC3 (NM_033089.7) was amplified from cDNA pool of HeLa cells by PCR and cloned into pEGFP-C3 (Takara Bio Inc., Shiga, Japan), pCMV-HA-N2 (Takara Bio Inc.,) ...