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Citations for Takara Bio :
3751 - 3800 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
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bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Biochemistry 2024Quote: ... PCR amplifications for synthetic genes and a linear-pET-22b (+) vector were performed with using PrimeSTAR® Max DNA polymerase (TaKaRa) and the designed primers (Supplementary Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
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bioRxiv - Biochemistry 2022Quote: ... KM881710.2) were PCR amplified from viral cDNA templates (31–33) and cloned into pSUMO expression plasmid by IN-FUSION (Takara Bio). The final constructs were confirmed by sanger sequencing performed by Genewiz.
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bioRxiv - Molecular Biology 2023Quote: ... and mmpL3 were amplified from Mtb H37Rv genomic DNA by PCR and inserted into pGW1-6C (gift of Tom Alber, University of California, Berkeley) by InFusion (Takara Bio) for wild-type genes or by ligation for recoded gene variants using restriction sites SpeI and NdhI ...
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bioRxiv - Microbiology 2023Quote: ... These identical sequences were generated via PCR with primers that contain a 5’ end identical to an adjacent segment and a 3’ end that anneals to the gene-of-interest sequence using a 2x hot-start PCR master mix (Takara, #R405A). The primers for PCR reactions were listed in Table 2.
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bioRxiv - Plant Biology 2022Quote: ... the full-length coding sequence of PLP with His6 at the N terminus was amplified by PCR and inserted into SalI-digested pBYR2HS vector using the In-Fusion Snap Assembly Master Mix (Takara Bio). The leaves of four-week-old N ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... A bead ratio of 1x was used (50 µL of AMPure XP beads to 50 µL cDNA PCR product with 1 µL of 10x lysis buffer added, as per Clontech instructions), and purified cDNA was eluted in 17 µL elution buffer provided by Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... A full-length Sfrp1 cDNA fragment was obtained by PCR amplifying mouse kidney cDNA using PrimeStar HS DNA polymerase (Takara Bio) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Genomics 2023Quote: ... a vector containing homology arms was generated by PCR-amplification of HAP1 gDNA and cloning of products into a linearized pUC19 backbone (InFusion, Takara Bioscience). Primers for these PCRs were designed such that homology arms would be between 200 bp and 1300 bp ...
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bioRxiv - Cell Biology 2023Quote: ... and of human FAM104B isoform 3 (NM_001166700.2) were PCR- amplified from a yeast two-hybrid human testis cDNA library (Clontech, cat. no. 638810) and cloned via appropriate restriction sites into pGAD-C1 (James et al ...
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bioRxiv - Genetics 2023Quote: ... Molecular cloning was performed using PCR to generate fragments with 15 bp overlapping ends and then assembling the fragments using In-Fusion Enzyme (Takara Bio) prior to transformation into chemically competent E ...
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bioRxiv - Cancer Biology 2023Quote: ... MYCN coding region was PCR amplified from HA-MYCN construct and cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: RACE-ready (Rapid Amplification of cDNA 5’ Ends) cDNA was generated using SMARTer PCR cDNA Synthesis Kit (#634925, Clontech Laboratories, Inc.) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was then subjected to the real time PCR for specific gene target by TB Green Premix Ex Taq (TaKaRa, RR420B) according to manufacturer’s instructions using Real Time PCR system (SIS-PCR005 ...
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR was performed using THUNDERBIRD Next SYRB qPCR Mix (TOYOBO) on a real-time PCR system (Thermal Cycler Dice® Real Time System, Takara).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the synthesized cDNA was used to check the expression of the GFP transcript by semiquantitative PCR using GTPCR master mix (TAKARA BIO) for the GFP-specific primer ...
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bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... containing VSW-3 RNA polymerase promoter for positive-sense and negative-sense strand ssRNA were amplified by a three-step PCR using PrimeSTAR Max DNA Polymerase (Takara, Japan) (primers are listed in Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was cloned between the 3’ and 5’ arms of the Rosa26 targeting vector using In-Fusion cloning (Takara Bio). Plasmid sequence was checked by sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR reactions were implemented in an Applied Biosystems 7500 Real-Time PCR system with the SYBR® Premix Ex TaqTM II (TAKARA). ACTIN was used as the reference gene for qPCR analyses ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
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bioRxiv - Neuroscience 2024Quote: The genomic region (813 bp) containing the Tbr2-crRNA target sequences was PCR amplified using PrimeSTAR Max (Takara Bio, Shiga, Japan), and a pair of primers ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were amplified from neuronal tissue cDNA libraries (see above) using TaKaRa LA Taq® DNA Polymerase (Takara Cat # RR002A) PCR products were purified using the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA fragments of approximately 1.5 kb upstream and downstream of LBDG_21500 required for homologous recombination were amplified via polymerase chain reaction (PCR; PrimeSTAR Max DNA polymerase, Takara, Shiga, Japan) using L ...
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bioRxiv - Microbiology 2024Quote: ... cDNA transcribed from 0.025 μg total RNA was used per PCR reaction in a final volume of 20 μl with TB Green Premix Ex Taq II (#RR820A, Takara Bio). PCR was performed using a Biorad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested chromatin was allowed to diffuse out of cells at 37 °C for 20 min and DNA was purified by Nucleospin gel and PCR clean-up spin column (Takara bio). Sequencing libraries were prepared using the KAPA HTP Library Preparation Kit (KAPA Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... the transcript levels of the corresponding genes were measured using quantitative real-time PCR by using TB Green™ Premix Ex Taq™ II (TaKaRa). The primers used are listed in Table S1.
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bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 ul from each samples were directly used for cDNA amplification (15 cycles of LD PCR, using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio, #634898) and the SeqAmp DNA Polymerase (Takara Bio #638509) ...
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bioRxiv - Developmental Biology 2020Quote: ... templates were constructed by cloning the 2 kb 5’ and 3’ homology arms into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). We used the CRISPR design tool (http://crispr.mit.edu ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR was alternatively performed for the cDNA using the SYBR® Premix Ex Taq™ (Tli RNase H Plus) (TAKARA-Clontech) using a QuantStudio5 system (Applied Biosystems) ...
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bioRxiv - Systems Biology 2019Quote: ... The relative 2 standard curves real-time PCR was carried out on the cDNA samples using TaqMan master mix (Takara, Otsu, Japan) and a Q-6000 machine (Qiagen ...
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bioRxiv - Microbiology 2019Quote: Genes encoding tail and baseplate proteins (ORF95–ORF102) in spontaneous mutant phages were amplified by plaque PCR using DirectAmpPCR (TAKARA, Shiga, Japan) and analyzed by Sanger sequencing ...
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bioRxiv - Developmental Biology 2019Quote: ... The SOX2 PCR product was purified and inserted into CMV-t2a-GFP using the In-Fusion HD cloning kit (Takara Bio USA) and transformed into Stellar Competent Cells (Takara Bio USA) ...
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bioRxiv - Immunology 2019Quote: ... by generating cDNA from 2 ng RNA and 9 PCR cycles per replicate with the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (cat# 634891, Takara) and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096 ...
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bioRxiv - Biophysics 2019Quote: ... Site-directed mutagenesis was achieved by polymerase chain reaction (PCR) of the full-length plasmid containing the Nav gene using PrimeSTAR® MAX DNA Polymerase (Takara Bio.). All clones were confirmed by DNA sequencing.
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bioRxiv - Molecular Biology 2019Quote: ... The resulting cDNA was subjected to real-time PCR analysis with the use of a Thermal Cycler Dice Real Time System (TAKARA BIO INC.). The PCR primers for medaka kitlga ...