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Citations for Takara Bio :
2751 - 2800 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The 50 µl PCR reaction contains 1.25 U PrimeSTAR GXL DNA Polymerase (Takara, R050), 1X PrimeSTAR GXL Buffer ...
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bioRxiv - Microbiology 2021Quote: ... and subjected to qRT-PCR analysis using Premix Ex Taq reagents (Takara, Dalian, China). Primers were referenced from previous reports (35 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... insert and vector fragments were generated by PCR using PrimeSTAR Max DNA Polymerase (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... bHLH100 and bHLH101 gene were PCR amplified and cloned into pGADT7-Rec vector (Takara) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified using NucleoSpin Gel and a PCR Clean-up kit (Takara-bio) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was subjected to PCR using ExTaq DNA polymerase (Takara Co., Otsu, Japan) or PrimeSTAR GXL DNA polymerase kit (Takara Co.) ...
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bioRxiv - Genomics 2020Quote: ... and 72°C for 5 s) on the PCR Thermal Cycler Dice (Takara Bio) using PrimeSTAR MAX DNA polymerase (Takara Bio) ...
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bioRxiv - Biochemistry 2019Quote: ... Quantitative real-time PCR was performed with SYBR Premix Ex Taq (TaKaRa, Shiga, Japan) using Thermal Cycler Dice® Real Time System Single (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... PCR amplification was performed using PrimeSTAR HS DNA polymerase (TAKARA Bio Inc., Kusatsu, Japan) with/without GC buffer for accurate amplification of GC rich targets ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were inserted using the In-Fusion reaction according to manufacturer’s protocol (Clontech).
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bioRxiv - Genetics 2019Quote: For high fidelity PCR reactions we followed manufacturer’s instructions using either Extaq (TaKaRa # RR001C), KOD hotstart (Merck Millipore # 71086 ...
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bioRxiv - Immunology 2020Quote: Mouse tail DNA was amplified using KAPA Taq PCR Kit (Takara Bio, cat. R040A) and primers Rosa26 WT F ...
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bioRxiv - Microbiology 2019Quote: Preparative DNA for plasmid cloning and PCR fusion constructs was amplified with CloneAmp (Takara) using the following cycle conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR product was cloned into the vector pIRESpuro2 using those restriction enzymes (Clontech).
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bioRxiv - Evolutionary Biology 2021Quote: ... We amplified NSP exon 1 from gDNA with EmeraldAmp MAX PCR Master Mix (TaKaRa), using primers specific for exon 1 (Table S3) ...
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bioRxiv - Neuroscience 2020Quote: ... The amplicon was purified using NucleoSpin Gel and PCR Clean-up (Takara Bio, 740609), reacted with one of the PCR primers and the BigDye terminator v3.1 cycle sequence kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The qRT-PCR was conducted with cDNA and TB Green premix (Takara, Japan, RR420A) or SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: The minigenes were generated by PCR-amplifying the desired sequences from genomic DNA (Clontech). Primers were designed for each minigene ...
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bioRxiv - Zoology 2021Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: All PCR reactions were performed in 50 μL reactions using ExTaq Polymerase (Takara Bio) with the following program:
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR reactions were subjected using TaKaRa SYBR Premix Ex Taq II (TaKaRa, Japan), and PCR processing was carried out in an iCycler (Bio-Rad ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The PCR product was ligated to pEGFP-C1 vector (Clontech Laboratories, Mountain View, CA), resulting in plasmid pEGFP-hUFL1 ...
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bioRxiv - Cell Biology 2021Quote: ... was cloned into pGIPZ-scarlet via PCR amplification and the InFusion cloning system (Takara).
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bioRxiv - Cancer Biology 2022Quote: ... Transcript expressions were determined by qRT-PCR using SYBR Premix Ex Taq II (TaKaRa) and the QuantStudioTM real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR polymerase capable of handling GC-rich amplicons was used (PrimeSTAR GXL Premix, Clontech). The resulting DNA amplicon ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was amplified by long distance PCR amplification using Advantage polymerase mix (TaKaRa). The amplified ds cDNA was purified using CHROMA SPIN TE-400 column which retains and traps the DNA molecules of less than 200 bp size ...
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bioRxiv - Immunology 2022Quote: ... the PCR product was assembled into an EcoRI + XhoI digested pMSCV-neo (Takara Clontech) vector by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was amplified by qRT-PCR with SYBR Premix Ex Taq II (RR820A, Takara) in ABI 7300 thermal cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: sgRNA sequences were amplified from gDNA by PCR using Ex Taq DNA polymerase (TaKaRa). PCR mixes were prepared with 10 µL 10X reaction buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (TliRNaseH Plus) (TaKaRa). The level of GmSALT3 transcript was normalised using the control gene GmUKN1 (Hu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Site-directed mutagenesis was performed by inverse PCR using PrimeSTAR GXL polymerase (Takara, Japan) and the pGEMR-T-vector as a template ...
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bioRxiv - Microbiology 2020Quote: ... a diagnostic PCR analysis was performed using LA Taq® DNA polymerase (Takara, Japan), the primers listed in table S2 and gDNA obtained from the Pfvps60 KO strain and compared with the WT 3D7 strain ...
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bioRxiv - Neuroscience 2021Quote: ... a series of degenerate PCR was performed with TaKaRa Ex Taq® (Takara Clontech) and primers ...
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bioRxiv - Neuroscience 2021Quote: ... a series of degenerate PCR was performed with TaKaRa Ex Taq® (Takara Clontech) and primers ...
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bioRxiv - Immunology 2020Quote: The real-time quantitative PCR was conducted with the Premix Ex Taq (Takara, Japan) to quantify the mRNA level ...
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bioRxiv - Neuroscience 2020Quote: Coding sequence of CROCCP2 was amplified by PCR using Prime Star DNA polymerase (TAKARA) from GW9 human fetal cortex cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified PCR product was inserted into EcoRI-linearized pLVX TetOne-puro plasmid (Clontech) using the NEBuilder HiFi Assembly kit (New England BioLabs) ...
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bioRxiv - Biochemistry 2021Quote: ... mutations were introduced into the RSF-OxlT vector via PCR using PrimeSTARMax (Takara Bio). Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... we performed RACE PCR using a SMARTer® RACE 5’/3’ Kit (Takara Bio), according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: ... The product was cloned into pET28a plasmid using In-Fusion PCR Cloning Kit (Clontech), with an N-terminal hexahistidine (His6 ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified with NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 74061) for Sanger sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The titer was measured using a Lenti-X qRT-PCR titration kit (Clontech 631235).
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bioRxiv - Developmental Biology 2022Quote: ... The qRT-PCR was performed with the cDNA using the SYBR-green stain (Takara). Primers used for qPCR are listed below.
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bioRxiv - Developmental Biology 2022Quote: ... The titer of retrovirus was measure by Retro-X qRT-PCR Titration Kit (Clontech). For iPSC generation ...
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bioRxiv - Immunology 2022Quote: ... the PCR product was assembled into an EcoRI + XhoI digested pMSCV-neo (Takara Clontech) vector by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Immunology 2022Quote: ... PCR amplicons were purified from 1% agarose gels following electrophoresis by column purification (Takara) and submitted for sanger sequencing through Genewiz (Primer ...
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bioRxiv - Cell Biology 2022Quote: qRT-PCR were performed using the SYBR Premix Ex Taq polymerase (Takara Bio Inc) with the ViiA™7 Instrument (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... Human B55α was PCR amplified from HUVEC cDNA and cloned into pEGFP-C1 (Clontech) using HindIII and BamHI sites ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... and cDNA was amplified by PCR (20–30 cycles) with Ex-Taq (Takara Bio) and specific primers (Table S1) ...
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bioRxiv - Pathology 2019Quote: ... Quantitative PCR (qPCR) was performed using TB GreenTM Premix Ex TaqTM II (TaKaRa, RR820A) on QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...