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Citations for Takara Bio :
2551 - 2600 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... we conducted additional long PCR using LA Taq polymerase (TaKaRa Bio Inc.) to detect occupied attB and confirm the functionality of primers used for detection of empty attB ...
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bioRxiv - Plant Biology 2022Quote: ... and qRT-PCR was performed on the Thermal Cycler Dice® (TaKaRa) using SYBR® Premix EX Taq™ and gene-specific primers designed in this study (Table 2 ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 10 μl reactions by TaKaRa Ex Taq ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 50 μl reactions by TaKaRa LA Taq ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were performed using TB Green Premix Ex Taq (Takara, USA). Gene expression was normalized against the average of two loading controls (Btf3 and Ppia).
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bioRxiv - Genomics 2023Quote: ... and extracted using the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) or Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... PCR conditions consisted in 0.05 U of PrimeSTAR GXL polymerase (Takara Bioscience), 250 nM of each primer ...
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bioRxiv - Developmental Biology 2024Quote: ... were used to PCR amplify the homology repair template (Takara PrimeStar Max). After purification (Promega Wizard SV Gel and PCR clean-up system) ...
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bioRxiv - Immunology 2024Quote: ... The two PCR products were cloned via In-Fusion HD Cloning (Takara) into the pCAGGS-ARM/GPC plasmid digested with XbaI and KpnI replacing a segment of the wild-type GPC sequence ...
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bioRxiv - Microbiology 2024Quote: ... 10 μl of 2x EmeraldAmp® GT PCR Master Mix (Takara Bio), 6 μl of ddH2O ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were performed with PrimSTAR GXL DNA polymerase (TaKaRa Bio USA) with 1 μl of genomic DNA and 0.2 μM primers ...
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bioRxiv - Bioengineering 2023Quote: ... 741-bp PCR product amplified from pCMV-mCherry-C1 (Clontech Laboratories, Inc.) using primers 5’-ATTACCGGTGTTAACATGGTGAGCAAGGGCGAGGA and 5’-TAAACCGGTCTTAAGTTACTTGTACAGCTCGTCCATG was cloned into AgeI-digested pAAV-JeT-DIO-MCS.
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL of EmeraldAmp® GT PCR Master Mix (Takara Bio, USA), 10 μM of each forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... Phusion High-Fidelity DNA Polymerase (Thermo) or CloneAmp HiFi PCR Premix (Takara) was used to PCR amplify all the above sequences with primers listed in Table S2 ...
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bioRxiv - Biochemistry 2022Quote: ... PCR was performed using a 0.25 U PrimeSTAR HS DNA polymerase (TaKaRa) with 0.2 mM dNTP mixture ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were amplified using PrimeSTAR GXL DNA polymerase (Takara Bio) under the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from RNA using SMARTer PCR cDNA synthesis kit (Clontech) per manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: PCR was performed using 1.25 U PrimeSTAR GXL DNA Polymerase (Takara, Japan) with 0.2 µM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2023Quote: Standard PCR reactions were performed using the PrimeSTAR Max DNA Polymerase (TaKaRa) or KOD One PCR Master Mix -Blue-(Toyobo) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were routinely tested for mycoplasma contamination by PCR (6601, Takara Bio). To convert primed hESCs to RSeT or PXGL culture conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was prepared with SYBR qPCR Premix (Takara, Japan, RR420L), and then performed with a real-time PCR detection instrument (Bio–Rad CFX96 ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-amplified donor sequences were independently cloned by In-fusion reaction (Takara) into the backbone of the Addgene plasmid 58409 which contains the homology arms (HA ...
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bioRxiv - Genetics 2023Quote: ... cDNA was amplified and purified using an Advantage 2 PCR Kit (Clontech). The cDNA library was sequenced using an Illumina sequencing platform (NovaSeq6000) ...
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bioRxiv - Genomics 2023Quote: ... a preliminary Terra PCR Direct Polymerase mix amplification (Takara Bio, Kusatsu, Japan) using 1µL of crude sample was used for direct genotyping without DNA purification ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... mtDNA was then amplified by PCR using PrimeSTAR® GXL polymerase (Takara). PCR was performed using two sets of slightly overlapping primers to reduce primer bias ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed with SeqAmp DNA Polymerase (Takara Bio; cat. no. 63850), and PCR products purified and concentrated with the QIAquick PCR Purification kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... the respective sequence was amplified by PCR from pEGFP C1 (Clontech, Takara Bio Europe ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was amplified using Emerald Amp GT PCR mix (Takara Bio, Japan) on Verti 96 well thermocycler (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and 25 μl PrimeSTAR Max PCR Readymix (Takara, Mountain View, CA, USA). PCR reactions were carried out by 30 cycles of denaturation at 98°C for 10 seconds ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification was first performed with PrimeSTAR GXL Premix (Takara Bio, R051A) to attach Nextera-style adapter sequences ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes and T4 DNA ligase from NEB and PCRs were done using the PrimeSTAR Max DNA polymerase from Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were performed using PrimeSTAR Max DNA Polymerase (Takara, Kusatsu) with primer pairs (Hokkaido System Science ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative PCR was performed with the SYBR Premix Ex TaqTM II (Takara) with the CFX96 Real-Time PCR System (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... The first-round PCR was performed using LA Taq DNA polymerase (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... then DNA was purified using a PCR cleanup spin column (Takara #74609). The sequencing library preparation was performed using NEBNext Ultra II DNA kit (#E7645S) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNAs were PCR-amplified using LA Taq DNA polymerase (TaKaRa), a common PCR primer used for all library amplifications ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Takara, Cat.# RR820) on a StepOnePlus system (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... on a Thermal Cycler Dice Real-Time PCR System (Takara Bio, Japan). Details regarding the primers for mRNA expressions of EPAS1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified PCR fragment was ligated into pMD20-T vector (Takara Bio). The Cas9 plasmid was used as a template for in vitro transcription using mMESSAGE mMACHINE T7 Ultra Kit ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed by TB Green Premix Ex Taq II (TAKARA) using gene specific primers and Thermal Cycler Dice Real Time System (TAKARA) ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR was performed using TB Green Premix Ex Taq II (TaKaRa) and the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were then ligated in then In-Fusion® reaction (Takara). Positive colonies were confirmed by Sanger sequencing (Source Bioscience ...
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bioRxiv - Bioengineering 2024Quote: ... Linear DNA fragments were produced by PCR using HiFi polymerase (Takara, 639298), followed by treatment with DpnI to remove template DNA ...
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bioRxiv - Bioengineering 2024Quote: ... qRT-PCR was performed using Takara SYBR Premix Ex Taq (Takara Bio) on Biorad CFX connect real-time system (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using TB Green PCR Master Mix (RR820, Takara Bio) with the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using the patS pRL fw and patS pRL rv infusion primers and cloned into the BamHI restriction site of the pRL1272-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pRL fw and hetL pRL rv infusion primers and cloned into the BamHI restriction site of the pRL25T-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Cancer Biology 2022Quote: ... The converted cDNA was amplified using gene-specific primers and TB Green® Premix Ex Taq™ II (Cat# RR820A; DSS Takara Bio India Private Ltd) to quantify the expression of the genes using a real-time PCR system (Applied Biosystem Quant Studio 7 Flex ...