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Citations for Takara Bio :
3001 - 3050 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and its contents were expelled into a PCR tube containing the lysis buffer (Takara, 634894). Metadata for all Patch-seq neurons including in this study are located in Supplementary Data Table 3.
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bioRxiv - Microbiology 2024Quote: Gaps or ambiguous nucleotide positions were corrected by (nested) PCR using TaKaRa EX Taq (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Quantitative PCR reactions were performed using the Takara TP-850 thermal cycler (Takara Bio, Japan) and THUNDERBIRD® SYBR™ qPCR Mix (Toyobo ...
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bioRxiv - Cell Biology 2024Quote: ... The guides were then PCR amplified for 26 cycles using the Ex Taq polymerase (Takara) protocol with the P5 forward primer mix (Takara ...
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bioRxiv - Genomics 2024Quote: ... Backbone preparation was run in 4x of 50 μL PCR using PrimeSTAR Max from Takara as described above ...
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bioRxiv - Genetics 2024Quote: ... Second strand synthesis and PCR amplification was done by adding Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... KODone PCR master mix and In-Fusion Snap Assembly Master Mix were purchased from Takara Bio Inc ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was purified using a NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) and eluted with 30 μl of 5 mM Tris-HCl buffer (pH 8.5) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR was performed using SYBR Premix Ex Taq II (Tli RNaseH Plus, TaKaRa) and monitored with the Thermal Cycler Dice Real Time System II (TaKaRa) ...
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bioRxiv - Cancer Biology 2021Quote: Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Genetics 2021Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TAKARA) and ViiA7 Real-Time PCR system ...
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bioRxiv - Developmental Biology 2021Quote: ... After PCR ssDNA was generated using the Guide-it Long ssDNA Production System (Takara Bio USA) using the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested PCR product was cloned into a TA-vector by Mighty TA-cloning Kit (Takara). After LacZ selection ...
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bioRxiv - Genomics 2022Quote: ... the cDNAs were amplified with an appropriate number of PCR cycles using LA Taq polymerase (Takara), and then the reactions were treated with 200 U/mL of Exonuclease I (NEB ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR reactions were performed using TB Green Premix Ex Taq reagent (TaKaRa, Cat#RR420A) on QuantStudio 7 Flex (Applied Biosystems) ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq™ II (Tli RNaseH Plus, TaKaRa). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Molecular Biology 2019Quote: ... GFI1B-BirA*:HA forms were expressed from PCR amplified sequences subcloned into pLVX-Tight-Puro (Clontech) with Not1/Mlu1 for doxycycline inducible expression with the Tet-On Advanced (Clontech ...
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bioRxiv - Genomics 2020Quote: ... Each sample was PCR amplified for 9 cycles using HS LA Taq (Takara, Mountain View, CA) and cleaned with 0.7X AMPure beads to remove small fragments and excess reagents (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... The contents of the pipette were expelled into a PCR tube containing lysis buffer (Takara, 634894). cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was conducted using SYBR Premix Ex Taq (Takara Bio Inc., Shiga, Japan) and a Thermal Cycler Dice Real Time System TP800 (Takara Bio Inc.) ...
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bioRxiv - Plant Biology 2020Quote: Real-time PCR was performed using TB Green Premix Ex Taq™ II (Takara, Dalian, China). An ABI 7500 real-time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... All modification for plasmids were performed using overlap PCR mutagenesis with InFusion cloning (Takara Biosciences, USA). Viral genome and support plasmids were maintained in chemically-competent Stbl2 E ...
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bioRxiv - Microbiology 2020Quote: ... Realtime PCR was performed using TB Green® Advantage® qPCR premix (Takara Bio USA, Inc.) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Semi-quantitative PCR was conducted using LA taq with GC buffer I (Takara medical co. ltd) using the methods in manual.
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bioRxiv - Microbiology 2020Quote: ... the extracted DNA was subjected to PCR using Takara Ex Taq Hot Start Version (Takara Bio) with the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... and assembled with PCR-amplified homology arms using In-Fusion® HD Cloning Kit (Takara 638920). Final products were verified by enzymatic digestion and sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The PCR-amplified elements were ligated in a single reaction step by In-Fusion cloning (Clontech) upstream of the HA3 tag in XmaI and AfeI sites of pLN-HA3-loxP ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplification were conducted using Ex Taq in accordance with the manufacturer’s instructions (TaKaRa Bio, Inc.).
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bioRxiv - Cell Biology 2021Quote: ... The real-time quantitative PCR analysis was performed using SYBR Premix Ex Taq II (TAKARA, China) on LightCycler 480 PCR System (Roche ...
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bioRxiv - Physiology 2022Quote: ... PCR reaction was conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primer pairs are listed in supplementary table S2.
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Microbiology 2022Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Microbiology 2022Quote: ... GISAID ID: EPI_ISL_408667)29 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... The 20 µL PCR reaction volume included 10 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... and then used to synthesize cDNA with TaKaRa RNA PCR™ Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Microbiology 2020Quote: ... were amplified by PCR using genome as template and cloned into T-Vector pMD19 (Simple, Takara). Primers containing the mutations were used to PCR the whole derived pMD19 plasmid constructed above ...
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bioRxiv - Immunology 2020Quote: ... real-time PCR analysis was carried out using Premix Ex Taq (probe qPCR) (Takara, Dalian, China). Real-time PCR was performed with WSSV-specific primers WSSV-RT1 (5’-TTGGTTTCATGCCCGAGA TT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... GISAID ID: EPI_ISL_408667)37 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
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bioRxiv - Synthetic Biology 2019Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq™ II (Tli RNaseH Plus, TaKaRa). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
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bioRxiv - Genetics 2019Quote: Regions orthologous to human placental igDMR were PCR-amplified with TaKaRa EX Taq HS (TaKaRa Bio) with primers specific for chimpanzee and rhesus macaque CGIs (Supplementary Table 4) ...
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bioRxiv - Genomics 2019Quote: ... and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA, RR820A). The sequences of the primers were listed in the Supplementary Table 4.
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bioRxiv - Physiology 2019Quote: ... PCRs for each sample were performed as triplicates with the SYBR Green dye (TaKaRa, Dalian, China) and StepOnePlus™ Real-Time PCR System according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
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bioRxiv - Cell Biology 2019Quote: ... qRT-PCR was carried out using TB Green™ Premix Ex Taq™ II (Takara, RR820A) and Roche Light Cycler 96 system (Roche) ...